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Pakistan Journal of Pharmaceutical Sciences. 2014; 27 (3): 487-490
in English | IMEMR | ID: emr-142163

ABSTRACT

A method was proposed to determine kaempferol and quercetin in Hippophae rhamnoides L medicinal preparation xindakang tablet by beta-cyclodextrin modified micellar electrokinetic capillary chromatography. Under the optimized conditions: buffer solution of 20 mmol/L Na[2]B[4]O[7]-KH[2]PO[4] [pH 9.0]-20mmol/L SDS-6mmol/L beta-CD-5%[v/v] MeCN, applied voltage of 16 kV and injection time of 8s, the two analytes were separated well within 10 minutes. The calibration was linear in the 0.02-0.80 and 0.02-0.70 mg/mL range for kaempferol and quercetin, respectively. The reproducibility based on migration time and peak height were 0.47% and1.87% for kaempferol, 0.55% and 2.02% for quercetin. The detection limits based on three times noise were 0.010 mg/mL and 0.008 mg/mL for kaempferol and quercetin, respectively. The developed method was utilized to analyze real samples and running recovery experiments with satisfactory results.


Subject(s)
Quercetin , beta-Cyclodextrins , Chromatography, Micellar Electrokinetic Capillary , Tablets
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