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1.
Article in Chinese | WPRIM | ID: wpr-922034

ABSTRACT

OBJECTIVE@#To explore the genetic basis for a Chinese pedigree affected with mental retardation.@*METHODS@#G-banded karyotyping analysis and single nucleotide polymorphism microarray (SNP array) were used to detect the genetic variants within the family, and the origin of the variants was analyzed using UPDtool Statistics software.@*RESULTS@#The patient, a 26-year-old female, was found to have a chromosomal karyotype of 46,XX,dup(4)(q28.2q31.3),and SNP array revealed a 25.71 Mb duplication at 4q28.2-q31.3. The duplication was inherited from her father, and her fetus was found to carry the same duplication.@*CONCLUSION@#The duplication of the patient probably underlay the mental retardation. The gender of the carrier and parental origin of the duplication might have led to the variation in their clinical phenotype.


Subject(s)
Adult , Chromosome Banding , Female , Genetic Testing , Humans , Karyotyping , Male , Pedigree , Trisomy/genetics
2.
Article in Chinese | WPRIM | ID: wpr-921665

ABSTRACT

Evodiamine, a bioactive indole alkaloid from Evodia rutaecarpa, E. rutaecarpa var. officinalis, or E. rutaecarpa var. bodinieri, has been extensively investigated due to its pharmacological activities in recent years. At present, evodiamine is proved to significantly suppress the proliferation of a variety of cancer cells and mediate cell processes such as cell cycle arrest and cell migration. In addition, evodiamine displays significant pharmacological activities against cardiovascular diseases(hyperlipidemia, etc.), and tinea manus and pedis. Recently, evodiamine has been found to have potential toxic effects, such as hepatotoxicity, nephrotoxicity, and cardiotoxicity. However, the pharmacological and toxicological mechanism of evodiamine is not clear, and its toxicity in vitro and in vivo has been rarely reported. Therefore, this study reviewed the pharmacological and toxicological articles of evodiamine in recent years, aiming at providing new ideas and references for future research.


Subject(s)
Evodia , Hand Dermatoses , Humans , Plant Extracts , Quinazolines/toxicity , Tinea
3.
Acta Pharmaceutica Sinica ; (12): 92-101, 2021.
Article in Chinese | WPRIM | ID: wpr-872627

ABSTRACT

The incidence of malignant melanoma, a highly fatal skin tumor, is on the rise worldwide. Melanomas are highly aggressive and have strong metastatic capability that leads to lethality. Recurrence occurs in patients with distant metastases, even with the latest treatments, and median survival is only a few months. At present, the prevention and treatment of melanoma includes surgical resection, chemotherapy, immunotherapy and targeted therapy. However, these strategies can lead to drug resistance and adverse effects. In recent years, an increasing number of studies have found that natural products have effective anti-melanoma activities, including inhibition of tumor growth, induction of cell apoptosis, inhibition of angiogenesis and metastasis and toxicity to tumor stem cells. In addition, several studies have reported that the combination of natural products and traditional anti-melanoma drugs can enhance the therapeutic efficacy. In this review we summarize the prevention and treatment of melanoma with natural products.

4.
Acta Pharmaceutica Sinica ; (12): 2241-2247, 2021.
Article in Chinese | WPRIM | ID: wpr-887050

ABSTRACT

This study investigated the intervention effect and possible mechanism of ophiopogonin D (OPD) in protecting cardiomyocytes against ophiopogonin D' (OPD')-induced injury, and provided relevant experimental data for the clinical use of Ophiopogon japonicas. Cell counting kit-8 (CCK-8) assay was used to evaluate the effect of OPD and OPD' on H9c2 cell viability. The content of reaction oxygen species (ROS) in cells were detected by flow cytometry. The contents of Fe2+ in cells were detected by FerroOrange's fluorescence imaging. The content of glutathione (GSH) and glutathione peroxidase (GSH-Px) were detected by kits. The expression of transferrin receptor 1 (TFR1), cyclooxygenase 2 (COX2), NADPH oxidase 1 (NOX1), long-chain acyl-CoA synthetase 4 (ACSL4), cationic amino acid transporter 11 (SLC7A11), glutathione peroxidase 4 (GPX4), and ferritin heavy chain 1 (FTH1) was detected by Western blot. Results showed that OPD' (1 μmol·L-1) significantly induced the expression of ferroptosis-related proteins, the contents of Fe2+, ROS, and GSH-Px were increased, and the content of GSH were decreased. In addition, different concentrations of OPD (0.5, 1, and 2 μmol·L-1) could partially reverse the myocardial cell injury caused by OPD', and the best effect was obtained when the dose range was 1-2 μmol·L-1. The experimental results show that OPD can interfere with the ferroptosis caused by OPD', and then have a protective effect on H9c2 cells.

5.
Article in Chinese | WPRIM | ID: wpr-885820

ABSTRACT

Objective:To evaluate the effect of vacuum disk(VD) for non-invasive treatment of recurrent and acquired pectus excavatum(PE).Methods:From June 2017 to June 2019, 29 patients recruited from our outpatient clinic were included in this retrospective study and followed-up every 3 month according to the schedule. The patients were distributed into three groups(group 1 treated ≤6 months; group 2 treated from 6 months to 12 months; group 3 treated >12 months). The device should be applied regularly for more than 2 hours daily. The deformity chest wall was scanned by three-dimensional(3D)scanner at clinic, and the 3D-depth(3D-DE) and 3D-Haller index(3D-HI) of PE were calculated through Geomagic software.Results:In this cohort, 29 patients were eligible, 18 symmetrical PE and 11 asymmetric PE. The application time ranged from 3 months to 15 months(average 7.6 months). 4 paitents was lifted to a normal level, 23 patients were differently improved. However, 2 paitents had no improvement. The average of the depth and 3D-HI of all patients were improved from 17.7 mm to 11.6 mm and 1.739 to 1.598, respectively. It’s no statistically significant difference for the elevation of 3D-DE and 3D-HI between symmetrical and asymmetric PE( t=-2.821, P=0.558; t=0.074, P=0.068). When comparing the improvement of 3D-DE or 3D-HI of PE to the patient's treatment time, a statistically significant difference was proved between the group 2 and group 1( t=-2.261, P=0.014; t=-0.436, P=0.043), but not between the group 3 and group 2( t=-1.240, P=0.139; t=0.622, P=0.568). The main side effects include moderate subcutaneous hematoma(84%), petechial bleeding(27%), thoracalgia(32%) and chest tightness(17%), no other side effect appear till now. Conclusion:VD for treatment of recurrent and acquired PE is convenient, safe and noninvasive, which can be an alternative treatment for recurrent and acquired PE, However, long term of efficacy evaluation is still needed.

6.
Article in Chinese | WPRIM | ID: wpr-884504

ABSTRACT

Radiation-induced skin injury refers to the acute and chronic skin damage caused when skin is exposed to radiation. Radiation-induced skin damage may be created by nuclear disaster, radiation accident, radiation therapy, occupational exposure and so on. Approximately 95% of patients receiving radiotherapy will eventually develop into radiation-induced dermatitis during or after the treatment. As a consequence, how to appropriately prevent and remedy radiation-induced skin injury is of great practical significance. According to traditional Chinese medicine, radiation-induced skin injury belongs to fire, heat and toxin, blocking Qi and blood, injuring the muscle surface, affecting the distribution of Qi, blood and body fluid in the body, and damaging the function of viscera. This paper summarizes the cognition and development of traditional Chinese medicine theory of radiation-induced skin injury, as well as the research progress of internal and external treatment of traditional Chinese medicine, so as to provide a basis for the research and treatment of radiation-induced skin injury with traditional Chinese medicine.

7.
Acta Pharmaceutica Sinica B ; (6): 727-737, 2021.
Article in English | WPRIM | ID: wpr-881165

ABSTRACT

The constitutive androstane receptor (CAR, NR3I1) belongs to nuclear receptor superfamily. It was reported that CAR agonist TCPOBOP induces hepatomegaly but the underlying mechanism remains largely unknown. Yes-associated protein (YAP) is a potent regulator of organ size. The aim of this study is to explore the role of YAP in CAR activation-induced hepatomegaly and liver regeneration. TCPOBOP-induced CAR activation on hepatomegaly and liver regeneration was evaluated in wild-type (WT) mice, liver-specific YAP-deficient mice, and partial hepatectomy (PHx) mice. The results demonstrate that TCPOBOP can increase the liver-to-body weight ratio in wild-type mice and PHx mice. Hepatocytes enlargement around central vein (CV) area was observed, meanwhile hepatocytes proliferation was promoted as evidenced by the increased number of KI67

8.
Article in English | WPRIM | ID: wpr-881064

ABSTRACT

Enterovirus 71 (EV71) infection is more likely to cause hand, foot and mouth disease (HFMD) in children, which can lead to neurogenic complications and higher mortality. As a commonly used clinical medicine, Reduning injection (RDN) helps to shorten the symptoms of patients with HFMD and facilitate the early recovery of children. However, the regulatory mechanism of RDN on the HFMD immune system disorder caused by EV71 remains to be discussed. This study collected detailed treatment data of 56 children with HFMD who entered the affiliated Children's Hospital of Nanjing Medical University during 2019. Retrospective analysis of clinical data showed that the symptoms of the RDN treatment group were improved compared with the untreated group. To explore its mechanism, the relevant detection indicators were detected by flow cytometry, enzyme-linked immunosorbent assay and real-time quantitative PCR. It was found that the number and function of innate immune (ILCs) and adaptive immunity (Th1, Th2 and secreted cytokines) were reduced, suggesting that RDN plays a role by regulating cellular immunity. The in vitro differentiation inhibition test further confirmed that RDN affected Th1 differentiation by inhibiting the expression of transcription factors on the basis of Th1 cell differentiation in vitro.

9.
Article in English | WPRIM | ID: wpr-880511

ABSTRACT

OBJECTIVE@#To observe the effect of Modified Xijiao Dihuang Decoction (, MXDD) on rats with radiation enteritis, and explore its action mechanism.@*METHODS@#Thirty female Sprague Dawley rats were divided into the control, model, dexamethasone (DXM), golden bifid (GB) and MXDD groups using random number table, 6 rats in each group. Except the control group, the other rats were developed into radiation enteritis model by exposing to a single @*RESULTS@#On day 1 to 3 after radiation, compared with the control group, the body weight in model group was decreased (P<0.05 or P<0.01). Compared with the model group, MXDD could alleviate weight loss and diarrhea caused by irradiation. At the phylum level, MXDD cause a significant increase in Firmicutes, and a decrease in Proteobacteria (P<0.05 or P<0.01). At the genus level, MXDD reduced the proportion of Escherichia Shigella (P<0.01). In addition, IL-17 and FoxP3 mRNA and protein expression levels were down-regulated and ROR-γt was up-regulated by MXDD treatment (P<0.05). Besides, Firmicutes and Lactobacillus were positively correlated with FoxP3 (r=0.73, 0.79, respectively; P<0.01), negatively correlated with IL-17 (r=0.66, 0.64, respectively; P<0.01 or P<0.05) and ROR-γt (r0.73, 0.81, respectively; P<0.01). Proteobacteria and Escherichia Shigella both had positive correlation with IL-17 (r 0.77, 0.57, respectively; P<0.01 or P<0.05 ) and ROR-γt (r=0.94, 0.79, respectively; P<0.01) and negative correlation with FoxP3 (r0.74, 0.65; P<0.01).@*CONCLUSION@#MXDD could improve the survival status of irradiated rats by regulating the richness, diversity and composition of intestinal flora, and restoring the balance of Th17/Treg.

10.
Article in Chinese | WPRIM | ID: wpr-888020

ABSTRACT

To explore the effect of ophiopogonin D on main fatty acid metabolic enzymes in human cardiomyocyte AC-16,so as to provide reference for cardiovascular protection mechanism and safe clinical application of Ophiopogon japonicus.CCK-8 (cell counting kit-8) was used to detect the effect of different concentrations of ophiopogonin D on the viability of cardiomyocytes.Meanwhile,the effect of different concentrations of ophiopogonin D on the morphology and quantity of cardiomyocytes was observed under microscope.The effect of ophiopogonin D on the mRNA expression of CYP2J2,CYP4F3,CYP4A11,CYP4A22 and CYP4F2 in cardiomyocytes was detected by RT-PCR.Western blot was used to detect the protein expression of CYP4F3 in different concentrations of ophiopogonin D.Compared with the control group,low-concentration ophiopogonin D had no effect on the viability of cardiomyocytes.However,ophiopogonin D with a concentration of higher than 20μmol·L~(-1)could promote the viability.Under the microscope,ophiopogonin D with a concentration of below 100μmol·L~(-1)had no significant effect on the morphology and number of cardiomyocytes.RT-PCR results showed that compared with the control group,5μmol·L~(-1)ophiopogonin D could slightly up-regulate mRNA expressions of CYP2J2 and CYP4F3,while high-concentration ophiopogonin D (10 and 20μmol·L~(-1)) could significantly induce mRNA expressions of CYP2J2and CYP4F3 in a dose-dependent manner (P<0.05).The same concentration of ophiopogonin D had a little effect on the mRNA expressions of CYP4A11,CYP4A22 and CYP4F2.Western blot results showed that 20μmol·L~(-1)ophiopogonin D could significantly induce the protein expression of CYP4F3 in a dose-dependent manner (P<0.05).Based on the above results,ophiopogonin D (less than100μmol·L~(-1)) has no effect on the viability of AC-16 cardiomyocytes.Ophiopogonin D (less than 100μmol·L~(-1)) can selectively induce the expressions of CYP2J2 and CYP4F3,regulate the metabolic pathway of fatty acid signaling molecules,and thus protecting the cardiovascular system.


Subject(s)
Fatty Acids , Humans , Myocytes, Cardiac , Saponins/pharmacology , Spirostans/pharmacology
11.
Article in Chinese | WPRIM | ID: wpr-906463

ABSTRACT

Objective:To observe the clinical efficacy of Jiechang Qingre pills for dampness-heat syndrome of large intestine at active stage of ulcerative colitis (UC) and investigate its effect on inflammatory factors. Method:One hundred and eight patients with active UC were divided into observation group and control group. Both groups were treated with Mesalazine enteric-coated tablets, 2 g/times, 2 times/day, for 2 weeks. If symptoms were poorly controlled, prednisone acetate tablets would be used instead, 0.75 mg·kg<sup>-1</sup>·d<sup>-1 </sup>in 3 times by oral administration. Patients in the observation group took Jiechang Qingre pills, 10 g/time, 3 times/day before meals. Patients in the control group took Jiechang Qingre pills simulated drug, 10 g/time, 3 times/day before meals. The course of treatment was 12 weeks in both groups and the patients were followed up for 3 months. The modified Mayo score was used to evaluate disease activity. Before and after treatment, large intestine dampness-heat syndrome score, inflammatory bowel disease questionnaire (IBDQ), mucosal histology assessment and scores of major symptoms and intestinal mucosal lesion severity were graded. The incidence of non-reactivity, hormone failure, hormone dependence, and early recurrence were recorded 2 weeks after treatment. Tumor necrosis factor-<italic>α </italic>(TNF-<italic>α</italic>), interleukin-6(IL-6) and IL-17 levels were measured before and after treatment. Result:The clinical effective rate in the observation group was 94.00% (47/50), higher than 77.55% (38/49) in the control group (<italic>χ</italic><sup>2</sup>=5.514,<italic>P</italic><0.05). The clinical remission rate was 82.00%(41/50) in the observation group, higher than 61.22% (30/49) in the control group (<italic>χ</italic><sup>2</sup>=5.266,<italic>P</italic><0.05). The endoscopic response rate was 96.00% (48/50) in the observation group, higher than 79.59% (39/49) in the control group (<italic>χ</italic><sup>2</sup>=6.251,<italic>P</italic><0.05). The rate of mucosal healing in the observation group was 90.00% (45/50), higher than 79.59% (35/49) in the control group (<italic>χ</italic><sup>2</sup>=5.503,<italic>P</italic><0.05). The scores of diarrhea, purulent stool, abdominal pain, tenesmus, hyperemia, edema, erosion and ulcer in the observation group were lower than those in the control group (<italic>P</italic><0.01). The rate of non-reactivity in the observation group was 16.00% (8/50), lower than 34.69% (17/49) in the control group(<italic>χ</italic><sup>2</sup>=4.581,<italic>P</italic><0.05). The hormone failure rate in the observation group was 37.50%(3/8), lower than 64.71%(11/17)in the control group,but the difference was not statistically significant(tested by the exact probaility method). The hormone dependence rate in the observation group was 12.50%(1/8), lower than 23.53% (4/17) in the control group,but the difference was not statistically significant(tested by the exact probaility method). The early recurrence rate in the observation group was 14.00% (7/50), lower than 32.65%(16/49) in the control group(<italic>χ</italic><sup>2</sup>=4.827,<italic>P</italic><0.05). The scores of Mayo, dampness and heat syndrome and Geboes index in the observation group were lower than those in the control group (<italic>P</italic><0.01), and the IBDQ scores were significantly higher than those in the control group (<italic>P</italic><0.01). The TNF-<italic>α, </italic>IL-6 and IL-17 levels of the patients in the observation group were lower than those in the control group (<italic>P</italic><0.01). Conclusion:Based on the routine treatment of western medicine, Jiechang Qingre pills treatment for the patients with active UC can effectively induce clinical remission, alleviate inflammatory reaction, promote intestinal mucosal healing, improve clinical symptoms, quality of life and the response of treatment. Its clinical efficacy and enteroscopy efficacy are better than western medicine treatment alone, so it is worthy of clinical use.

12.
Article in English | WPRIM | ID: wpr-903958

ABSTRACT

Bladder cancer is one of the most common types of cancer. Most gene mutations related to bladder cancer are dominantly acquired gene mutations and are not inherited. Previous comparative transcriptome analysis of urinary bladder cancer and control samples has revealed a set of genes that may play a role in tumor progression. Here we set out to investigate further the expression of two candidate genes, centromere protein U (CENPU) and mitochondrial ribosomal protein s28 (MRPS28) to better understand their role in bladder cancer pathogenesis. Our results confirmed that CENPU is up-regulated in human bladder cancer tissues at mRNA and protein levels. Gain-of-function and loss-of-function studies in T24 human urinary bladder cancer cell line revealed a hierarchical relationship between CENPU and MRPS28 in the regulation of cell viability, migration and invasion activity. CENPU expression was also up-regulated in in vivo nude mice xenograft model of bladder cancer and mice overexpressing CENPU had significantly higher tumor volume. In summary, our findings identify CENPU and MRPS28 in the molecular pathogenesis of bladder cancer and suggest that CENPU enhances the progression of bladder cancer by promoting MRPS28 expression.

13.
Article in English | WPRIM | ID: wpr-896254

ABSTRACT

Bladder cancer is one of the most common types of cancer. Most gene mutations related to bladder cancer are dominantly acquired gene mutations and are not inherited. Previous comparative transcriptome analysis of urinary bladder cancer and control samples has revealed a set of genes that may play a role in tumor progression. Here we set out to investigate further the expression of two candidate genes, centromere protein U (CENPU) and mitochondrial ribosomal protein s28 (MRPS28) to better understand their role in bladder cancer pathogenesis. Our results confirmed that CENPU is up-regulated in human bladder cancer tissues at mRNA and protein levels. Gain-of-function and loss-of-function studies in T24 human urinary bladder cancer cell line revealed a hierarchical relationship between CENPU and MRPS28 in the regulation of cell viability, migration and invasion activity. CENPU expression was also up-regulated in in vivo nude mice xenograft model of bladder cancer and mice overexpressing CENPU had significantly higher tumor volume. In summary, our findings identify CENPU and MRPS28 in the molecular pathogenesis of bladder cancer and suggest that CENPU enhances the progression of bladder cancer by promoting MRPS28 expression.

14.
Article in Chinese | WPRIM | ID: wpr-846465

ABSTRACT

Objective: To develop an accurate and effective method to identify the original animals of Chinese medicine Pheretima (Dilong in Chinese) and its adulterants by using mitochondrial genes, cytochrome coxidase I (CO I) and 16 S rRNA as DNA barcoding sequences to certify and improve morphological analysis. Methods: A total of 66 samples were initially identified according to their morphological characteristics, and then their CO I and 16 S rRNA sequences were simultaneously amplified for DNA barcoding. The two gene sequences were amplified with improved primers. One-step double PCR was used and the experimental conditions were optimized. The genetic distance among and within species of Pheretima and its hybrids was calculated by MEGA 5.1. The N-J tree was constructed based on K2P model. Results: Combined with morphological identification, CO I and 16S rRNA double DNA barcoding identification can accurately identify Pheretima and its adulterants. Conclusion: Morphological identification is the basis of molecular identification. Molecular identification can be a powerful supplement to the morphological identification. The combination of molecular identification and morphological identification can increase the accuracy of the identification of the original animals of Pheretima and its adulterants. Double DNA barcoding can also provide a solution for the identification of the medicinal materials of Pheretima and other animal medicinal materials.

15.
Article in Chinese | WPRIM | ID: wpr-829953

ABSTRACT

Objective To design, synthesize and measure antifungal activities of galloyl piperazine derivatives. Methods Trimethoxyl gallic acid was used as starting material, reacted with piperazine in the presence of PyBOP/DIEA to afford the intermediates. The target compounds were obtained through the reaction with corresponding acids after deprotection gave. The antifungal activities of the target compounds were evaluated by FLC-resistant Candida albican isolated according to the CLSI recommended method. Results 11 target compounds were synthesized and six of them showed more potent antifungal activities than gallic acid. Conclusion Galloyl piperazine derivatives could enhance antifungal activities. Galloyl moiety was an important pharmacophore, which could improve antifungal activities with the introduction of cinnamic acid and 2,3-dichlorobenzoic acid.

16.
Article in Chinese | WPRIM | ID: wpr-873159

ABSTRACT

Objective:To investigate the effect and mechanism of ginsenoside Rg1(G-Rg1)in ameliorating lipid uptake and oxidation in HepG2 cells induced by free fatty acids (FFA). Method:HepG2 cells were divided into normal group, model group,low-dose ginsenoside Rg1 group (25 μmol·L-1) and high-dose G-Rg1 group (50 μmol·L-1). HepG2 cells were treated with 1 mmol·L-1 free fatty acid for 24 h to construct the NAFLD cell model, and then treated with 25,50 μmol·L-1 G-Rg1 for 24 h. The effect of G-Rg1 on HepG2 cell activity was determined by cell counting kit-8(CCK-8) assay. The level of triglyceride (TG) was detected by micro method. The accumulation of lipid droplets was observed by oil red O staining. Quantitative real-time fluorescence polymerase chain reaction (Real-time PCR) and Western blot were used to detect the alterations of key genes and proteins relating to lipid uptake and metabolism. Result:Compared with the normal group, the intracellular TG level and the absorbance of the oil red O staining in the model group were significantly increased (P<0.01). Compared with the model group, G-Rg1 reduced TG and lipid deposition were significantly reduced (P<0.01).Results of Real-time PCR and Western blot showed that compared with normal group, model group peroxisome proliferators-activated receptors gamma(PPARγ),fatty acid binding protein 1(FABP1),fatty acid transport protein 2/5(FATP2/5)and fatty acid translocase(CD36)expressions increased(P<0.05),whereas peroxisome proliferators-activated receptors α(PPARα),carnitine palmitoyltransferase 1(CPT1)and peroxisomal acyl-coenzyme A oxidase 1(ACOX1)expressions decreased(P<0.05). Compared with the model group, the expressions of PPARγ, FABP1, FATP2, FATP5 and CD36 in the G-Rg1 group were decreased (P<0.05,P<0.01), while the expressions of PPARα, CPT1 and ACOX1 were increased (P<0.05,P<0.01). Conclusion:G-Rg1 can ameliorate lipid deposition in NAFLD cell model by reducing lipid uptake and increasing lipid oxidation.

17.
Article in Chinese | WPRIM | ID: wpr-872100

ABSTRACT

Objective:In response to the outbreak of severe infectious diseases such as new coronavirus pneumonia, an artificial intelligence based image diagnosis system is established to improve the efficiency of disease diagnosis, reduce the burden of front-line doctors, and improve the medical resource allocation.Methods:Using the deep convolution neural network and regional prevention and control auxiliary information system, the image data and other information of the confirmed patients were analyzed and processed comprehensively.Results:A set of AI based medical image auxiliary data processing system is proposed. Combined with multimodal medical data collaborative diagnosis, it can effectively and accurately segment the diseased areas in patients' lung CT images, and generate standardized reports. Relying on the multi-center collaborative diagnosis and treatment platform, the system can introduce multi-expert remote consultation mechanism to improve the diagnosis quality of severe patients.Conclusions:By segmenting pathological regions, generating standardized reports and introducing multicenter mechanism, the system can help to optimize the medical resources allocation and improve the utilization of these resources.

18.
Article in Chinese | WPRIM | ID: wpr-870725

ABSTRACT

The effects and practicability of in situ simulation and standardized patient (SP) in residency training of general practice was evaluated in Hangzhou First People′s Hospital. From September 2017 to December 2019, typical cases were collected in accordance with the teaching objectives, the teaching plans of in situ simulation were established and simulation scenarios were set up; the SPs were recruited from nursing staff and retirees in the community. Forty general practice residents were randomly divided into two groups with 20 in each group. The traditional teaching group was given theoretical teaching, while the combined teaching group was taught by in-situ simulation combined with SP teaching. The satisfaction of residents, the scores of theory and skill were observed and compared between two groups.Results showed that the satisfaction of teaching method( t=-2.11, P<0.05), teaching effect( t=-4.25, P<0.01) and scores of history collection( t=-3.21, P<0.01) in the combination teaching group were significantly higher than those in the traditional teaching group. It is suggested that in situ simulation combined with SP teaching method can improve the satisfaction and teaching effect of the general practice residents.

19.
Article in Chinese | WPRIM | ID: wpr-870615

ABSTRACT

The mentor system has been applied in the standardized residency training, currently the "one to one" ortwo mentors′ system are more common applied. However, the clinical training of general practitioners needs joint effort of mentors from the general practice department and specialists in hospital, as well as of mentors from primary health institutes. Thus, we proposed a "three-in-one" tutorial system, which consisted of one GP mentor, one specialists in the hospital and one primary physician for residency training of general practice. The implementation of the "three-in-one" system has motivated the enthusiasm of specialists and community instructors, and enhanced their responsibility. The mentors from different department can learn from each other and complement each other, so the overall teaching level can be raised and the quality of standardized training of general practitioners can be improved.

20.
Article in Chinese | WPRIM | ID: wpr-870001

ABSTRACT

Pheochromocytoma/paraganglioma(PPGL) was a kind of neuroendocrine tumor that derived from chromaffin tissue, which seems to be an important etiology of secondary hypertension. With the development of molecular detection technology, at least 17 kinds of pathogenic genes of PPGL has been discovered, which is related to 35%-40% PPGL, and about 40% malignant PPGL is associated with SDHB gene mutation. In this study, we reported a case with a novel splicing mutation of SDHB gene induced paraganglioma.

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