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1.
Rev. bras. med. esporte ; 28(2): 123-125, Mar-Apr. 2022. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1365690

ABSTRACT

ABSTRACT Introduction: Proper physical exercise can improve the body's breathing and blood circulation, improve immune function, and help relaxation. As a result, negative emotions are effectively guided. Objective: To explore the impact of exercise on subhealth states. Method: This paper analyzes the exercise ability of college students with chronic fatigue syndrome, records the relevant data, and compares them with healthy students. In the comparative analysis, we found that there were significant differences in physiological indexes and satisfaction scores between the experimental group and the control group. Results: Through the analysis of the significant difference data, it can be concluded that the muscle endurance and cardiopulmonary function of college students with chronic fatigue syndrome are significantly lower than the normal value. Conclusion: Proper and regular exercise can treat chronic fatigue syndrome. We found that exercise therapy can achieve ideal results in our long-term tracking of subjects' data. Level of evidence II; Therapeutic studies - investigation of treatment results.


RESUMO Introdução: Atividade física adequada pode aprimorar a respiração e a circulação sanguínea do corpo, melhorar a função imune e ajudar no relaxamento. Assim, é possível direcionar emoções negativas efetivamente. Objetivos: Explorar o impacto da atividade física em estados de saúde não ideais. Método: Esse trabalho analisa o preparo físico de estudantes universitários com síndrome da fadiga crônica, registrando os dados relevantes e comparando-os com estudantes saudáveis. Através dessa comparação, descobrimos que há diferenças significativas em índices fisiológicos e scores de satisfação entre o grupo experimental e o grupo controle. Resultados: Através da análise das diferenças significativas dos dados, pôde-se concluir que a resistência muscular e a função cardiopulmonar de estudantes colegiais com síndrome da fadiga crônica são significativamente inferiores aos valores normais. Conclusão: A atividade física adequada e regular pode ser um tratamento para a síndrome da fadiga crônica. Descobrimos que a terapia por meio da atividade física pode atingir resultados ideais através de um acompanhamento de longo prazo dos dados registrados dos participantes. Nível de evidência II; Estudos terapêuticos - investigação do resultado de tratamentos.


RESUMEN Introducción: Actividad física adecuada puede mejorar la respiración y la circulación sanguínea del cuerpo, optimar la función inmune y ayudar en la relajación. Así, es posible dirigir emociones negativas efectivamente. Objetivos: Explorar el impacto de la actividad física en estados de salud no ideales. Método: Este trabajo analiza la preparación física de estudiantes universitarios con síndrome de fatiga crónica, registrando los datos relevantes y comparándolos con estudiantes saludables. A través de esta comparación, descubrimos que hay diferencias significativas en índices fisiológicos y puntuaciones de satisfacción entre el grupo experimental y el grupo control. Resultados: A través del análisis de las diferencias significativas de los datos, se puede concluir que la resistencia muscular y la función cardiopulmonar de estudiantes colegiales con síndrome de fatiga crónica son significativamente inferiores a los valores normales. Conclusión: La actividad física adecuada y regular puede ser un tratamiento para el síndrome de la fatiga crónica. Descubrimos que la terapia por medio de la actividad física puede alcanzar resultados ideales a través de un acompañamiento a largo plazo de los datos registrados de los participantes. Nivel de evidencia II; Estudios terapéuticos - investigación del resultado de tratamientos.

2.
Article in Chinese | WPRIM | ID: wpr-913067

ABSTRACT

Objective To analyze the epidemiological characteristics of reported imported malaria cases in Zhengzhou City from 2016 to 2020, so as to provide insights into the management of imported malaria in the city. Methods All data pertaining to cases with definitive diagnosis of malaria in Zhengzhou City from 2016 to 2020 were captured from the National Notifiable Disease Report System and the Information Management System for Parasitic Disease Control in China, including individual demographic data, and malaria onset, initial diagnosis and definitive diagnosis data. All data were descriptively analyzed. The duration from malaria onset to initial diagnosis, from initial diagnosis to definitive diagnosis and from onset to definitive diagnosis was compared among cases. In addition, the diagnoses of imported malaria cases in which definitive diagnosis was made were compared with the reexaminations by Zhengzhou Municipal Malaria Diagnosis Reference Laboratory. Results A total of 302 cases with definitive diagnosis of malaria were reported in Zhengzhou City from 2016 to 2020, and all were imported cases, with Plasmodium falciparum malaria as the predominant type (230 cases, 76.2%). There were 293 malaria cases imported from Africa (293 cases, 97.0%), which mainly included Nigeria (48 cases, 15.9%), Angola (40 cases, 13.2%), and the Democratic Republic of the Congo (29 cases, 9.6%). There was no obvious seasonality found in the date of malaria onset and time of reporting malaria. The ratio of male to female malaria cases was 49.3:1, and there were 103 cases (34.1%) with the current residency address in Zhengzhou City, 193 cases (63.9%) with the current residency address in other cities of Henan Province and 6 cases (2.0%) in other provinces of China. There were 271 cases (89.7%) seeking initial diagnosis in medical institutions, and the diagnostic accuracy of malaria was 56.6% (171/302) at initial diagnosis institutions. A total of 122 cases (40.4%) sought medical care on the day of malaria onset, and 252 cases (86.4%) within 3 days; however, only 22 cases (7.3%) were definitively diagnosed on the day of onset, and 162 cases (53.6%) diagnosed within 3 days. There were no significant differences between malaria cases seeking initial diagnosis at medical institutions and disease control and prevention institutions in terms of the duration from malaria onset to initial diagnosis (Z = −1.663, P > 0.05), from initial diagnosis to definitive diagnosis (Z = −0.413, P > 0.05) or from malaria onset to definitive diagnosis (Z = −0.838, P > 0.05). The median duration (interquartile range) from initial diagnosis to definitive diagnosis of malaria was 3.00 (2.00), 3.00 (6.00), 2.00 (4.00) d and 1.00 (1.00) d among cases seeking medical care at township-level and lower, county-, city- and province-level medical institutions, and the median duration from initial diagnosis to definitive diagnosis of malaria was significantly longer among cases seeking medical care at township-level and lower medical institutions than at city (Z = −3.286, P < 0.008 33) and province-level medical institutions (Z = −9.119, P < 0.008 33), while the median duration from initial diagnosis to definitive diagnosis [1.00 (3.00) d vs. 2.00 (4.00) d; Z = −4.099, P < 0.016] and from malaria onset to definitive diagnosis [3.00 (4.00) d vs. 4.00 (5.00) d; Z = −2.868, P < 0.016] among malaria cases with the current residency address in Zhengzhou City was both shorter than in other cities of Henan Province. The diagnostic accuracy was 89.1% (269/302) among malaria cases in which definitive diagnosis was made, and the accuracy of malaria reexaminations was 94.0% (284/302) in Zhengzhou Municipal Malaria Diagnosis Reference Laboratory. Conclusions P. falciparum malaria was predominant among reported imported malaria cases in Zhengzhou City from 2016 to 2020, and these imported malaria cases were predominantly diagnosed at medical institutions; however, the diagnostic capability of malaria is poor in township-level and lower medical institutions. Strengthening the collaboration between medical institutions and disease control and prevention institutions and improving the diagnostic capability building at medical institutions are recommended to consolidate malaria elimination achivements.

3.
Article in Chinese | WPRIM | ID: wpr-888215

ABSTRACT

Long non-coding RNA (lncRNA) Dnm3os plays a critical role in peritendinous fibrosis and pulmonary fibrosis, but its role in the process of cardiac fibrosis is still unclear. Therefore, we carried out study by using the myocardial fibrotic tissues obtained by thoracic aortic constriction (TAC) in an early study of our group, and the


Subject(s)
Fibroblasts , Fibrosis , Humans , Myocardium/pathology , RNA, Long Noncoding , Signal Transduction , Transforming Growth Factor beta1
4.
Article in Chinese | WPRIM | ID: wpr-828147

ABSTRACT

Calnexin is a lectin-like molecular chaperone protein on the endoplasmic reticulum, mediating unfolded protein responses, the endoplasmic reticulum Ca homeostasis, and Ca signals conduction. In recent years, studies have found that calnexin plays a key role in the heart diseases. This study aims to explore the role of calnexin in the activation of cardiac fibroblasts. A transverse aortic constriction (TAC) mouse model was established to observe the activation of cardiac fibroblasts , and the cardiac fibroblasts activation model was established by transforming growth factor β1 (TGFβ1) stimulation. The adenovirus was respectively used to gene overexpression and silencing calnexin in cardiac fibroblasts to elucidate the relationship between calnexin and cardiac fibroblasts activation, as well as the possible underlying mechanism. We confirmed the establishment of TAC model by echocardiography, hematoxylin-eosin, Masson, and Sirius red staining, and detecting the expression of cardiac fibrosis markers in cardiac tissues. After TGFβ1 stimulation, markers of the activation of cardiac fibroblast, and proliferation and migration of cardiac fibroblast were detected by quantitative PCR, Western blot, EdU assay, and wound healing assay respectively. The results showed that the calnexin expression was reduced in both the TAC mice model and the activated cardiac fibroblasts. The overexpression of calnexin relieved cardiac fibroblasts activation, in contrast, the silencing of calnexin promoted cardiac fibroblasts activation. Furthermore, we found that the endoplasmic reticulum stress was activated during cardiac fibroblasts activation, and endoplasmic reticulum stress was relieved after overexpression of calnexin. Conversely, after the silencing of calnexin, endoplasmic reticulum stress was further aggravated, accompanying with the activation of cardiac fibroblasts. Our data suggest that the overexpression of calnexin may prevent cardiac fibroblasts against activation by alleviating endoplasmic reticulum stress.

5.
Article in Chinese | WPRIM | ID: wpr-743596

ABSTRACT

Objective To evaluate the effect of PAD bundle in mechanically ventilated patients for preventing delirium. Methods Two hundred and forty three patients with mechanical ventilation who were admitted in ICU from January 2015 to February 2018 were divided into two groups according to the method of random number table. The patients in the control group(120 cases) received routine nursing care. The patients in the experimental group(123 cases) received the PAD bundle on the basis of routine nursing care. The scores of Richards Campbell Sleep Questionnaire(RCSQ), the doses of sedatives and analgesics, incidence of delirium, duration of delirium, ventilator time, ICU length of stay and the 28-day ICU mortality were compared between the two groups. Results The incidence of delirium, duration of delirium, ventilator time, ICU length of stay was 23.58%(29/123), (2.83±1.20) d, (153.07±55.61) d, (7.88± 2.89) d in the experimental group, 40.00%(48/120), (4.02 ± 1.99) d, (170.90 ± 63.51) d, (10.13 ± 3.41) d in the control group, and there was significant difference between the two groups (χ2=8.298, Z=2.840, 2.276, 5.325, P <0.01 or 0.05). The total score of RCSQ was (55.23 ± 17.89) points in the experimental group, (51.86±16.45) points in the control group, and there was significant difference between the two groups(Z=5.654, P<0.05). There was no significant difference in 28-day ICU mortality between the two groups (P>0.05). Conclusions The PAD bundle can decrease the incidence of delirium, the duration of delirium and improve prognosis of the patients with mechanical ventilation.

6.
Article in Chinese | WPRIM | ID: wpr-733427

ABSTRACT

Objective To study the correlation between preoperative frailty and short-term prognosis after abdominal surgery, and to explore its predictive value. Methods A total of 156 elderly patients undergoing abdominal surgery from July 2017 to December 2017 in Tianjin Third Center Hospital were prospectively observed and classified into non-frail group, pre-frail group and frail group according to the Fried's frailty phenotype before surgery. The postoperative hospital stay and incidence of adverse outcomes were compared among the three groups, and the effect of frailty on the risk prediction model of the American Society of Anesthesiology (ASA) was analyzed. Results The postoperative hospital stay and the incidence of adverse outcomes in the three groups were statistically significant (P<0.05). Negative binomial regression analysis found that preoperative frailty was associated with prolonged postoperative hospital stay (pre-frail incidence rate ratio was 1.428 (95% CI : 1.250-1.974);frail incidence rate ratio was 3.692 (95% CI : 1.282-4.230) .Logistic regression analysis found that preoperative frailty was associated with an increased risk of postoperative adverse outcomes (pre-frail: OR=2.303(95% CI : 1.810-4.127); frail: OR=3.512 (95% CI : 2.054-5.689)). Frailty increased the diagnostic value of the ASA risk prediction model (Z=3.718, P<0.01). Conclusions Frailty of elderly patients is an important predictor of short-term prognosis of abdominal surgery, and it increases the diagnostic value of ASA risk model. The preoperative frailty assessment of elderly patients undergoing abdominal surgery is helpful for preoperative clinical decision-making and prognosis evaluation.

7.
Article in Chinese | WPRIM | ID: wpr-233766

ABSTRACT

<p><b>OBJECTIVE</b>To assess the value of magnetic resonance (MR) myocardial perfusion imaging (MRMPI) in evaluating the myocardial viability in patients with myocardial infarction.</p><p><b>METHODS</b>MRMPI was performed in 51 patients with myocardial infarction using a 1.5 T MR scanner. All the patients were examined using IR-turbo FLASH sequence during the first-pass and delayed phase 5-30 min after injection of 0.1 mmol/kg Gd-DTPA at the rate of 4 ml/s. The short axis images were acquired during the first-pass, and both the short axis and long axis images were obtained during the delayed phase. The left ventricular wall on the short-axis slice was divided into 8 segments. A correlative study of the results of the rest and stress (99m)Tc single photon emission computed tomography (SPECT) was carried out in 21 patients.</p><p><b>RESULTS</b>In the 51 patients with myocardial infarction, 42(82.3%) showed hypoperfusion during the first-pass imaging and 50(98%) had delayed hyperenhancement. In the 21 patients receiving SPECT, 48 nonviable segments was detected among the 168 segments scanned by (99m)TcSPECT, and MRMPI showed delayed hyperenhancement in all the infracted areas. Of the 120 viable segments detected by rest and stress (99m)Tc SPECT, 97 segments (80.8%) were found to be free of delayed hyperenhancement by MRMPI. With the rest and stress (99m)Tc SPECT as the reference, the sensitivity and the specificity of MRMPI were 100.0% and 80.8%, respectively.</p><p><b>CONCLUSION</b>MRMPI allows effective identification of the myocardial viability and nonviability as well as the severity and extent of the myocardial infraction.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Coronary Angiography , Female , Gadolinium DTPA , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Myocardial Infarction , Diagnosis , Diagnostic Imaging , Myocardial Perfusion Imaging , Methods , Sensitivity and Specificity , Tomography, Emission-Computed, Single-Photon
8.
Article in Chinese | WPRIM | ID: wpr-340776

ABSTRACT

<p><b>OBJECTIVE</b>To optimize scan delay time of multi-slice spiral CT portal venography (MSCTP) using test bolus injection.</p><p><b>METHODS</b>Sixty individuals with no hepatic diseases were randomly divided into 4 groups (A, B, C and D). The time-density curves (TDC) of the portal vein (PV) were acquired by test bolus (15 ml, 5 ml/s) at the level of liver hilus. In the 4 groups, the MSCTP were delayed for 4, 6, 8 and 10 s according to the peak time of TDC, respectively. The maximum CT value of the portal vein and liver parenchyma were recorded. The maximum intensity projection (MIP) and volume rendering (VR) were employed for three-dimensional reconstruction and the image quality of the 4 groups was estimated.</p><p><b>RESULTS</b>The average peak time of healthy individuals ranged between 24 and 32 s (95% confidence interval) by means of the test bolus (15 ml). Group C (delay time of 8 s) had a much better image quality of the portal vain than the other groups, and the small branches of the portal vein (6th and 7th orders) were clearly visualized; the major portal vein branches (1st to 4th orders) were also enhanced with sharp edges. Although the hepatic vein was also observed in the portal venous phase in group D, the details of the portal vein on the hepatic edge were distinct and well defined.</p><p><b>CONCLUSION</b>At the injection rate of 5 ml/s, the optimum scan time delayed is 8 to 10 s in normal individuals according to the peak time of the test bolus.</p>


Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Phlebography , Methods , Portal Vein , Diagnostic Imaging , Reproducibility of Results , Time Factors , Tomography, Spiral Computed , Methods , Young Adult
9.
Article in Chinese | WPRIM | ID: wpr-340754

ABSTRACT

<p><b>OBJECTIVE</b>To establish New Zealand rabbit hepatoma models with VX2 cell line, and modify the hepatic artery catheterization technique.</p><p><b>METHODS</b>Forty New Zealand rabbit models bearing hepatoma were established by implanting VX2 cells into the left and right liver lobes. Two weeks after the tumor cell implantation, 26 rabbits in the experimental group underwent modified hepatic artery catheterization procedures using microsurgical technique, and 10 rabbits in the control group were catheterized with 3F micro-catheter using Seldinger technique. The VX2 hepatomas were observed before and after the catheterization with multi-slice spiral CT scan and digital subtractive angiography (DSA).</p><p><b>RESULTS</b>Tumor growth after the tumor cell implantation was confirmed in 36 rabbits by CT scans and open operations. The success rate of catheterization was 88% (23/26) in the experimental group, and 40% (4/10) in the control group. VX2 hepatomas appeared as hypointense or isointense nodules on multi-slice spiral CT, and hepatic artery angiography showed that VX2 hepatomas had homogeneous or nodular tumor staining.</p><p><b>CONCLUSION</b>The modified hepatic artery catheterization using microsurgical technique has higher success rate than catheterization with 3F micro-catheter by Seldinger technique, and significantly decreases X-ray exposure for the staff undertaking the operations.</p>


Subject(s)
Animals , Carcinoma, Hepatocellular , Pathology , Catheterization , Methods , Cell Line, Tumor , Disease Models, Animal , Female , Hepatic Artery , Liver Neoplasms, Experimental , Pathology , Male , Rabbits , Radiography, Interventional , Tomography, Spiral Computed
10.
Chinese Journal of Surgery ; (12): 27-29, 2008.
Article in Chinese | WPRIM | ID: wpr-237839

ABSTRACT

<p><b>OBJECTIVE</b>To study the surgery plan and simulation effect of the three dimensional (3D) hepatic virtual operation based on the data of 64-slice helical CT scanning and to probe the feasibility of the virtual operation based on the FreeForm Modeling System.</p><p><b>METHODS</b>The volunteer liver was scanned to collect two dimensional (2D) DICOM data of 64-slice helical CT scanning and the 3D hepatic and intrahepatic vessels model were reconstructed by MIMICS software. The reconstructed liver, the intrahepatic vessels model and the artificial tumor models were output into the FreeForm Modeling System in the STL format. The device PHANTOM with the characterization of dynamo-feedback was applied to make the operation on the 3D hepatic.</p><p><b>RESULTS</b>The spatial relationship between the tumour and the intrahepatic vessels were clearly observed by rotation and enlargement of the target. According to the operation principle, the left lobe of liver resection was simulated by manipulating the device PHANToM. Through the liver transparence surface, the intrahepatic vessels were easily distinguished. The operation procedure was accord with the clinic hepatic surgery. Meanwhile, during the operation, by adjusting the incision objective intensity, the dynamo-feedback intensity was definitely touched.</p><p><b>CONCLUSIONS</b>By using the FreeForm Modeling System,the hepatic operation procedure can be simulated ahead of time. The operation complication in the practical surgery can be anticipated and the individualization operation schema can be reasonable instituted.</p>


Subject(s)
Adult , Feasibility Studies , Female , Hepatectomy , Methods , Humans , Imaging, Three-Dimensional , Methods , Liver , Diagnostic Imaging , Liver Neoplasms , General Surgery , Tomography, Spiral Computed , Methods , User-Computer Interface
11.
Article in Chinese | WPRIM | ID: wpr-293381

ABSTRACT

<p><b>OBJECTIVE</b>To study the segmentation methods of the liver CT images and the value of 3-dimensional (3D) reconstruction of the liver in the planning of hepatic surgery.</p><p><b>METHODS</b>The 2D Digital Imaging and Communications in Medicine (DICOM) format data of the liver obtained from healthy volunteers were transformed into bmp format image, and the liver image segmentation was performed using Photoshop software. The 3D model was reconstructed using MIMICS software.</p><p><b>RESULTS</b>The DICOM format data of the liver obtained by 64 slice spiral CT included totally 658 slice images. The segmented liver image showed clear profiles and complete intrahepatic duct data were reserved. The segmented liver images were free of discontinuation during continuous observation. The liver surface and internal ductal system, including the hepatic arteries and veins, and the hepatic portal system and their branches, were represented clearly. The reconstructed liver allowed clear identification of the anatomic landmark and matched the actual liver volume. The reconstructed ductal structure were distinct and continuous with natural texture. The reconstructed liver and the hepatic internal duct system were simultaneously displayed by adjusting the transparency of the liver, and the blood vessels were also represented.</p><p><b>CONCLUSION</b>Segmentation of the liver images in different phases using Photoshop can be feasible for liver reconstruction. The reconstructed liver and the intrahepatic ductal structure allow vivid 3D observation of the spatial relationship among the major tracts and accurate estimation of the liver volume.</p>


Subject(s)
Adult , Female , Humans , Image Interpretation, Computer-Assisted , Methods , Image Processing, Computer-Assisted , Methods , Imaging, Three-Dimensional , Methods , Liver , Diagnostic Imaging , Tomography, Spiral Computed , Methods
12.
Chinese Journal of Surgery ; (12): 909-912, 2007.
Article in Chinese | WPRIM | ID: wpr-340891

ABSTRACT

<p><b>OBJECTIVE</b>To study the value of three-dimensional 64-slice helical computer tomography (64-MSCT) in diagnosis and surgical treatment on diseases of digestive system.</p><p><b>METHODS</b>From November 2005 to August 2006, 64-MSCT was performed on 102 patients suspected of digestive diseases to display foci of infection, hepatic conduit, biliary-pancreatic duct and portal venous system, followed by three-dimensional reconstruction of those targets. The reconstructed images as well as the data obtained from the reconstruction were compared with the surgical results.</p><p><b>RESULTS</b>Twenty-five of 27 patients with portal hypertension (PHT) showed blur-free imaging of main portal vein (MPV) while the other 2 cases showed congenital cavernous transformation or thrombogenesis of MPV. Compensatory circulation of portal venous system was displayed satisfactorily in 27 cases. The three-dimensionally reconstructed images of 34 cases of hepatocellular carcinoma (HCC) demonstrated no invasion of tumors into the tissues and blood vessels of 23 cases, visible invasion into portal vein and hepatic veins of 9 cases, tumor embolus in portal veins of 1 case, and arterio-venous fistula in only one case. The 3D imaging of biliary passages displayed total concretion, tumor and constrictive or dilated biliary-pancreatic duct and bile duct around amphi-obstruction, which complied with the operations research of calculus of bile duct in 25 cases, cholangiocarcinoma in 5 cases, and cancer of pancreas in 5 cases. Operations research in accordance with the 3D reconstructed imaging accorded with imaging diagnosis in respect of PHT, HCC and diseases of biliary-pancreatic system at the rates of 100%, 97% and 100%, respectively.</p><p><b>CONCLUSIONS</b>Three-dimensional 64-MSCT displays foci of infection and constructions of biliary-pancreatic duct and hepatic conduit stereoscopically.</p>


Subject(s)
Adolescent , Adult , Aged , Digestive System , Diagnostic Imaging , Pathology , Digestive System Diseases , Diagnosis , Female , Humans , Imaging, Three-Dimensional , Male , Middle Aged , Sensitivity and Specificity , Tomography, Spiral Computed , Methods
13.
Article in Chinese | WPRIM | ID: wpr-255318

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the feasibility of dynamic magnetic resonance diffusion tensor imaging (DTI) in rabbit models with implanted VX2 hepatic tumor grafts.</p><p><b>METHODS</b>MRI and DTI images were obtained from 16 rabbit models with implanted VX2 hepatic tumor grafts (14, 18, 22, and 26 days after tumor implantation, respectively) and 4 normal rabbits. The average diffusion coefficient (ADC) and exponential ADC (eADC) were estimated and compared against pathological findings.</p><p><b>RESULTS</b>The ADC values increased after tumor implantation but then decreased in the rabbit models, whereas eADC exhibited a pattern of reverse changes. These changes significantly differed from those in the control group. Coagulation necrosis and fibrous hyperplasia showed obvious increase as found by pathological examination.</p><p><b>CONCLUSION</b>Dynamic MR DTI quantitative analysis of rabbit models of implanted VX2 hepatic tumor can partially describe the growth behaviors of implanted liver cancer.</p>


Subject(s)
Animals , Carcinoma, Hepatocellular , Pathology , Diffusion Magnetic Resonance Imaging , Female , Image Enhancement , Methods , Liver Neoplasms , Pathology , Male , Neoplasm Transplantation , Rabbits , Random Allocation
14.
Article in Chinese | WPRIM | ID: wpr-280709

ABSTRACT

The aim of this study was to compare and analyze protein staining in order to select the optimal staining method for proteomic research. Proteins from acute promyelocytic leukemia cell line NB4 and protein molecular weight marker were separated respectively by 2-D or 1-D electrophoresis and detected respectively by the typical Coomassie brilliant blue, the colloidal Coomassie brilliant blue, the modified Coomassie brilliant blue and the silver staining protocols. The protein detection sensitivity, compatibility with mass spectrometry (MS) and facility of the four staining protocols were compared. The results indicated that the silver staining exhibited the highest sensitivity and MS showed the lowest compatibility 10% of protein identification rate. The detection sensitivity of the modified Coomassie brilliant blue staining was superior to that of other two Coomassie brilliant blue stainings, close to but lower than the silver staining, however the compatibility with MS was better (protein identification rate about 55%). It is concluded that the protein detection sensitivity of the modified Coomassie brilliant blue staining is high, and its compatibility with MS is better, this modified Coomassie brilliant blue staining is an optimal staining method for proteomic research.


Subject(s)
Coloring Agents , Electrophoresis, Gel, Two-Dimensional , Methods , Humans , Leukemia, Promyelocytic, Acute , Metabolism , Mass Spectrometry , Methods , Neoplasm Proteins , Rosaniline Dyes , Silver Staining , Staining and Labeling
15.
Article in Chinese | WPRIM | ID: wpr-282964

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the possibility of dynamic magnetic resonance (MR) perfusion-weighted imaging (PWI) in rabbit models of implanted hepatic VX2 tumor.</p><p><b>METHODS</b>MRI and PWI images were obtained from 16 rabbit models of implanted hepatic VX2 tumor and 4 normal rabbits at 14, 18, 22, and 26 days after tumor implantation, respectively, and the mean time to enhancement (MTE) and maximum slope of increase (MSI) were estimated and compared with pathological changes.</p><p><b>RESULTS</b>In the rabbit models of implanted hepatic tumor the MTE tended to increase and MSI to descend 14, 18, 22, and 26 days after tumor implantation, showing significant differences from the control group. Coagulation necrosis and fibrous hyperplasia in the tumors appeared to progress with time as found pathologically.</p><p><b>CONCLUSION</b>Dynamic MR PWI quantitative analysis of rabbit models of implanted hepatic VX2 tumor provides valuable means for observing the growth behaviors of VX2 tumor and may be clinically applicable in the evaluation of hepatic diseases.</p>


Subject(s)
Animals , Contrast Media , Female , Image Interpretation, Computer-Assisted , Methods , Liver Neoplasms, Experimental , Diagnosis , Magnetic Resonance Angiography , Methods , Male , Rabbits , Random Allocation
16.
Article in Chinese | WPRIM | ID: wpr-282927

ABSTRACT

<p><b>OBJECTIVE</b>To improve the method for preparing rabbit VX2 liver tumor model and observe the magnetic resonance imaging (MRI) features of the implanted tumors.</p><p><b>METHODS</b>Sixteen adult New Zealand white rabbits were assigned randomly into 4 equal groups, and VX2 tumor tissues were implanted into the right and left liver lobes with spiral CT guidance. Plain and contrast-enhanced MR scan and pathological analysis were performed in different stages (14, 18, 22 and 26 days) after tumor implantation.</p><p><b>RESULTS</b>Tumor implantation was successful in all the rabbits, and 18 to 22 days after tumor implantation, the diameters of the tumor ranged from 1 to 2 cm, which allowed observation and study. In plain MR scans, lower or equivalent tumor signal in comparison with hepatic parenchyma was observed, and contrast-enhanced scans produced obvious enhancement of the tumor edges. At 22 days after tumor implantation, obvious necrosis was observed in the center of the tumor.</p><p><b>CONCLUSION</b>This method of preparing rabbit VX2 liver tumor model with spiral CT guidance is simple and convenient, and the tumors can be observed effectively with dynamic plain and contrast-enhanced MR scans.</p>


Subject(s)
Animals , Disease Models, Animal , Female , Liver Neoplasms, Experimental , Diagnostic Imaging , Pathology , Magnetic Resonance Imaging , Methods , Male , Neoplasm Transplantation , Rabbits , Random Allocation , Tomography, X-Ray Computed
17.
Chinese Journal of Oncology ; (12): 165-167, 2004.
Article in Chinese | WPRIM | ID: wpr-271027

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the characteristics of MR imaging of hepatic lesions using measurement of apparent diffusion coefficient (ADC) value of hepatic lesions on diffusion weighted imaging.</p><p><b>METHODS</b>MR diffusion weighted images were obtained in patients with 97 hepatic lesions (22 hepatocellular carcinomas, 21 metastatic tumors, 28 hemangiomas, 26 cysts). ADC values were evaluated with different sequences. The ADC ratio of lesion/liver was estimated.</p><p><b>RESULTS</b>Average ADC values of hepatic lesions were as follows: carcinomas (0.91 +/- 0.07) x 10(-3) mm(2)/s, metastatic tumors (1.13 +/- 0.27) x 10(-3) mm(2)/s, cavernous hemangiomas (1.94 +/- 0.37) x 10(-3) mm(2)/s, cysts (3.26 +/- 0.30) x 10(-3) mm(2)/s. The ADC ratio of lesion/liver was significantly different between primary carcinomas and metastatic tumors (P < 0.05).</p><p><b>CONCLUSION</b>Quantitative study in hepatic lesions using ADC values and the ADC ratio of lesion/liver, would improve the accuracy in diagnosing hepatic lesions.</p>


Subject(s)
Cysts , Diagnosis , Diagnosis, Differential , Diffusion , Hemangioma, Cavernous , Diagnosis , Humans , Liver , Pathology , Liver Neoplasms , Diagnosis , Magnetic Resonance Imaging
18.
Article in Chinese | WPRIM | ID: wpr-735448

ABSTRACT

Objective To investigate the significances of telomerase activity and P53 expression in Non-small cell lung cancer (NSCLC). Methods The activity of telomerase in cancerous tissues was detected by PCR-ELISA, and the expression of P53 protein was detected by S-P method. Results The telomerase activity and P53 expression were significantly higher in NSCLC tissues than in normal lung tissues. The telomerase activity and P53 expression had close association with the differentiation of cancer cells and lymph node metastasis. Conclusion It is very valuable to measure telomerase activity and P53 expression in studying the initiation and progression of NSCLC.

19.
Article in Chinese | WPRIM | ID: wpr-736916

ABSTRACT

Objective To investigate the significances of telomerase activity and P53 expression in Non-small cell lung cancer (NSCLC). Methods The activity of telomerase in cancerous tissues was detected by PCR-ELISA, and the expression of P53 protein was detected by S-P method. Results The telomerase activity and P53 expression were significantly higher in NSCLC tissues than in normal lung tissues. The telomerase activity and P53 expression had close association with the differentiation of cancer cells and lymph node metastasis. Conclusion It is very valuable to measure telomerase activity and P53 expression in studying the initiation and progression of NSCLC.

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