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1.
Chinese Journal of School Health ; (12): 1647-1649, 2019.
Article in Chinese | WPRIM | ID: wpr-815697

ABSTRACT

Objective@#To survey mental health knowledge and its influence factors among undergraduates in Henan provenience and to provide a scientific evidence for further formulating the targeted strategies.@*Methods@#Totally 840 agriculture and forestry college students, literature and history students, science students and medical students were selected with stratified multistage random sampling from 45 colleges and universities in Henan provenience,investigation of Mental Health related knowledge by self-designed questionnaire.@*Results@#The awareness rate of mental health knowledge was 75.86%(32 806/43 248). Multivariate linear regression analysis showed that score of mental health knowledge of femal students was 1.03 of the of male students; score of medical students was 5.19,3.65,2.65 of that of students in the other majors; score of students who obtained mental health knowledge through other channels(television/movie/network/talking) was 1.42 of that of than those who obtained through formal approaches(slogans/manuals/broadcast/cathedra)(P<0.05).@*Conclusion@#The awareness rate of mental health knowledge among undergraduates in Henan provenience need to be improved, the education of male, senior students, non-medical students and the explore of diversified forms should be strengthened.

2.
Chinese Journal of School Health ; (12): 1533-1535, 2019.
Article in Chinese | WPRIM | ID: wpr-816585

ABSTRACT

Objective@#To investigate the impacts of sports game curriculum on the physical development of preschool children aged 3 to 5 years, so as to provide a reference for sports education and intervention among preschool children.@*Methods@#Totally 176 preschool children aged 3 to 5 years in the large, medium and small class of a kindergarten in Changzhou were selected by means of cluster random sampling, and they were randomly divided into observation group (n=85) and control group(n=91). The control group did not receive special intervention, while the observation group received sports game curriculum, the intervention time was 1 year. The body shape and physical fitness indicators were tested and compared before and after intervention.@*Results@#In terms of body shape, the increase in height of the observation group was (6.57±0.41)cm, which was significantly higher than (5.77±0.36)cm of the control group(t=13.78, P<0.01); There was no significant difference in weight gain between the observation group and the control group (2.53±0.35)kg vs (2.61±0.43)kg(t=1.35, P>0.05). The physical fitness indicators, including standing long jump, continuous jumping of the feet, sit-and-reach, tennis throwing, single foot balance and 10 m×2 shuttle run effects were (95.93±4.02)cm, (6.72±0.35)s, (11.65±1.33)cm, (6.21±1.50)m, (45.26±8.74)s,(6.91±0.13)s in the observation group, significantly better than (82.71±3.59)cm, (7.52±0.41)s, (10.06±1.32)cm, (5.34±1.21)m, (36.14±6.72)s, (7.36±0.17)s in the control group(t=23.04, 13.88, 7.96, 4.25, 7.79, 19.63, P<0.05).@*Conclusion@#The sports game curriculum has no significant effect on the weight of preschool children aged 3 to 5, but it can promote the height growth of children and helps to improve physical fitness.

3.
Chinese Journal of Biotechnology ; (12): 616-625, 2019.
Article in Chinese | WPRIM | ID: wpr-771347

ABSTRACT

Glucoamylase is a critical ingredient for saccharification in the starch decomposition, and widely used in food, pharmaceutical and fermentation industries. Glucoamylases are usually thermostable and have peak activities at high temperature, as required for the industrial process of glucose production. In this study, a glucoamylase gene belonging to the glycoside hydrolase (GH) family 15, Tlga15A, was cloned from Talaromyces leycettanus JCM12802, and successfully expressed in Pichia pastoris GS115. Recombinant glucoamylase TlGA showed optimal activities at pH 4.5 and 75 °C. The result of thermostability analysis showed that TlGA retained above 70% activity after incubating for 1 h at 65 °C, and 43% residual activity after 30 min at 70 °C. Moreover, TlGA had high resistance to most metal ions and chemical reagents tested. Various starch substrates could be hydrolyzed by TlGA, including soluble starch (255.6±15.3) U/mg, amylopectin (342.3±24.7) U/mg, glycogen (185.4±12.5) U/mg, dextrin (423.3±29.3) U/mg and pullulan (65.7±8.1) U/mg. The primary, secondary and tertiary structures of glucoamylase were further analyzed. The low ratio of Gly in the primary structure and low exposed nonpolarity solvent accessible surface in the tertiary structure may be the main reasons for TlGA's thermostability. These results show that TlGA is great promising for potential use in the commercial production of glucose syrups. Moreover, this research will provide knowledge and innovating ideas for the improvement of glucoamylase thermostability.


Subject(s)
Cloning, Molecular , Enzyme Stability , Glucan 1,4-alpha-Glucosidase , Hydrogen-Ion Concentration , Pichia , Talaromyces , Temperature
4.
Chinese Pharmacological Bulletin ; (12): 915-921, 2017.
Article in Chinese | WPRIM | ID: wpr-620134

ABSTRACT

Aim To investigate the protective effect of astragaloside IV (AS-Ⅳ) on human retinal pigment epithelium injury induced by methylglyoxal (MGO), and explore its molecular mechanism.Methods The injury of ARPE-19 cells was induced by MGO and the cell viability was measured by CCK-8 method.The morphology of cell nucleus was analyzed by Hoechst 33342 staining and the cell apoptosis was analyzed by flow cytometry to detect labbled Annexin V-FITC/PI.JC-1 staining and fluorescence probe DCFH-DA were employed to evaluate the change of mitochondrial membrane potential and reactive oxygen species (ROS).The levels of SOD, MDA, caspase-9 and caspase-3 were determined by respective kits.Western blot was used to analyse the expression of Bcl-2, Bax and PARP.Results AS-Ⅳ could significantly inhibit the decrease of cell viability induced by MGO, improve the morphology of cell nucleus, reduce the ARPE-19 cell apoptosis rate and the level of ROS and MDA, and increase the activity of SOD.Furthermore, AS-Ⅳ could enhance mitochondrial membrane potential, the ratio of Bcl-2/Bax and the expression of PARP, and inhibit the activation of caspase-9 and caspase-3.Conclusion AS-Ⅳ may protect ARPE-19 cells from the injury induced by MGO by increasing the antioxidant ability of ARPE-19 cells and inhibiting cell apoptosis.

5.
Article in Chinese | WPRIM | ID: wpr-608087

ABSTRACT

This paper introduced the concept and prevalence of frailty, as well as the concept and mechanism of exercise therapy. For the frailty, there were various modes of exercise therapy, and several exercise prescriptions were reported.

6.
China Pharmacy ; (12): 2678-2680,2681, 2016.
Article in Chinese | WPRIM | ID: wpr-605199

ABSTRACT

OBJECTIVE:To optimize the ethanol mixed-extraction technology of Red ginseng and Salvia miltiorrhiza in Xin-likang granules. METHODS:L9(34)orthogonal test was adopted to optimize ethanol mixed-extraction technology with ethanol vol-ume fraction,amount of ethanol and extraction times as factors using weighting coefficient comprehensive score of the contents of ginsenoside Rg1,Re and Rb1 in R. ginseng and the contents of tanshinone ⅡA and salvianolic acid B in S. miltiorrhiza as index;and the verification test was detected. RESULTS:Optimal mixed-ethanol extraction technology was as follows as 6-fold 70% etha-nol,reflux extracting for 3 times,2 h each time. In verification test,average contents of ginsenosides Rg1,Re,Rb1 in R. ginseng and those of tanshinone ⅡA and salvianolic acid B in S. miltiorrhiza were 3.963 8,0.757 3,4.986 2,0.964 7,27.662 5 mg/g,re-spectively,and comprehensive score was 0.96 (RSD=1.26%,n=3). CONCLUSIONS:Ethanol extraction technology of R. gin-seng and S. miltiorrhiza in Xinlikang granules optimized by multiple indexes comprehensive score combined with orthogonal test is stable,reasonable and feasible.

7.
Article in Chinese | WPRIM | ID: wpr-493049

ABSTRACT

Objective To understand the status of tobacco epidemic among adults in Zhejiang province in 2013.Methods A total of 2 779 residents aged 15-69 years from 15 counties were selected using stratified multi-stage cluster sampling method and surveyed by face to face interview to complete questionnaire.Results The current smoking prevalence was 25.62% among the adults 15-69 years old in Zhejiang province,50.88% among males and 0.78% among females.The daily smoking prevalence was 21.85%;40.56% of the current daily smokers smoked within 30 minutes after waking;95.19% of current smokers were not willing to quit;64.04% of all respondents reported someone smoked in their own homes;67.48% smokers thought they would continue to smoke even if the price of tobacco rises;21.09% of all respondents saw tobacco AD at shops and on TV;42.00% of people were aware that smoking could cause stroke,heart attack and lung cancer;89.69% smokers still believed that low-tar cigarettes were less harmful than regular cigarettes;73.92% of people agreed with completely no-smoking in public places.Conclusions Male smoking prevalence in Zhejiang remains at a high level,coupled with low rate of quitting,second hand smoke exposure remains a serious issue in household,low awareness of the harms of smoking for people,suggesting that the task of controlling smoking needs to be strengthened.

8.
Chinese Journal of Immunology ; (12): 37-41, 2016.
Article in Chinese | WPRIM | ID: wpr-492039

ABSTRACT

Objective:To observe the effects of allogeneic compact bone derived-mesenchymal stem cells ( CB-MSCs) on pro-liferation and differentiation of T cells,and investigate the molecular mechanisms of the immunosuppressive ability.Methods:With an established co-culture system of CB-MSCs and mouse spleen lymphocytes ( SP) in vitro,we observed the effects of CB-MSCs on prolif-eration,apoptosis and cell cycle of SP by MTS/PES assay and flow cytometry.Also,we measured the effects of CB-MSCs on regulatory T cells ( Treg) ratio and expressions of CCR5,CCR7 and CXCR3 in SP.Results:CB-MSCs could obviously inhibit the PHA-stimulated SP proliferation with a dose-dependent manner;MSCs could significantly inhibit the spontaneous apoptosis of SP and induce SP cell cycle G0/G1 phase arrest.After co-culture with SP,CB-MSCs could obviously increase the proportion of Treg in SP,down-regulate the expression of CXCR3 and CCR5,as well as up-regulate the expression of CCR7.Conclusion: Allogeneic CB-MSCs can significantly inhibit cell proliferation of SP,the mechanisms mainly involved the G0/G1 cell cycle arrest rather than apoptosis induction.In addition, CB-MSCs can exert immunomodulatory effects by increasing the Treg ratio,regulating the expressions of chemokine receptors.

9.
China Pharmacy ; (12): 2530-2531,2532, 2015.
Article in Chinese | WPRIM | ID: wpr-605125

ABSTRACT

OBJECTIVE:To establish a method for the content determination of berberine hydrochloride in the Jiangtang xiao-zhi tablets. METHODS:HPLC was conducted with the Symmetry C18 column. The mobile phase was acetonitrile-0.05 mol/L sodium dihydrogen phosphate(pH adjusted to 3.0 using phosphoric acid)(24∶76,V/V)at the flow rate of 1.0 ml/min. The detection wave-length was 345 nm,the column temperature was room temperature and injection volume was 10 μl. RESULTS:The linear range of berberine hydrochloride was 0.522-4.698 μg(r=0.999 9);RSDs of precision,stability and reproducibility tests were no more than 0.72%;the average recovery was 97.79%(RSD=2.09%,n=6). CONCLUSIONS:The method is simple,accurate,sensitive and reproducible,and can be used for the content determination of berberine hydrochloride.

10.
Chinese Pharmacological Bulletin ; (12): 1704-1708, 2014.
Article in Chinese | WPRIM | ID: wpr-458764

ABSTRACT

Aim To investigate the optimum trypsin digestion time and the optimum trypsin concentration of the long-term formalin-fixed retina in rats.Methods Male SD rats were sacrificed by cervical dislocation. The removed eyes were fixed in 4% formaldehyde fixa-tive for 2 days,2 weeks,4 weeks,3 months,5 months and 7 months respectively.Fixed retinas at dif-ferent time were separated and put in 3%,6% and 9% trypsin at 37℃ in incubator.The time required was recorded for complete digestion of retinas,which fixed for different time and digested in different con-centrations of trypsin.The retinal blood vessels were recorded under a microscope after PAS staining of stretched retinal blood vessel.Results After conven-tionally fixed for 48 hours,retinas were digested com-pletely in 3% trypsin at 37℃ for 3.5 hours.The di-gestion time extended to 9 hours and 1 4 hours for the 2 weeks and 1 month fixed eyes,using 3% trypsin, which achieved the same result as conventional fixed eyes.For the 3 months fixed eyes,a complete retinal blood vessel could also be attained by using conven-tional concentrations of trypsin,and only by extending digestion time to 22 hours.It is also indicated that the increased concentration of trypsin did not shorten the time of digestion.In addition,for the fixed eye from 5 months to 7 months,6% and 9% trypsin could short-en,to some extent,the time to 22 hours and 28 hours respectively,which was conductive to obtaining rela-tively complete retinal blood vessels.Conclusions Retinal tissues which fixed time shorter than 3 months can completely be digested using 3% trypsin.Howev-er,for retinal tissue,which fixed from 5 months to 7 months,both the extending time of digestion and the increasing concentration of trypsin to 6% are necessary to get optimum retinal vascular digestion.

11.
Article in Chinese | WPRIM | ID: wpr-431618

ABSTRACT

Objective To study the status quo of the caring character of baccalaureate nursing students and its related factors.Methods A total of baccalaureate nursing students were recruited from two and three levels of baccalaureate in Nantong district by convenience sampling method and cluster sampling method.The students were investigated with a nurse caring character questionnaire which was developed in the previous study and then an analysis was conducted.Results The caring characters of the nursing baccalaureate students were at the medium level.The caring characters of baccalaureate nursing students were affected by various factors.The linear regression revealed that choosing nursing willingly or not,relationship with familyand sex,among other things had the greatest influence on their caring characters.Conclusions Therefore,importance should be attached to the caring character of baccalaureate students and corresponding guidance and education directed at the various grades should be offered so as to increase their caring characters.

12.
Journal of Leukemia & Lymphoma ; (12): 569-571, 2011.
Article in Chinese | WPRIM | ID: wpr-471794

ABSTRACT

MCL-1 gene is a antiapoptotic gene belong to BCL-2 gene family, its product MCL-1 protein plays an important role in cell apoptosis regulating and the course of hematologic malignancies.This paper reviews advance of studies on the function of MCL-1 gene and MCL-1 protein,the role of MCL-1 protein in hematologic malignancies and the relationship between MCL-1 protein and other apoptosis adjustment factors.

13.
Article in Chinese | WPRIM | ID: wpr-387769

ABSTRACT

Objective To explore the anxious condition and the influencing factors of chronic hepatitis B patients, and to take appropriate care measures. Methods Self-designed questionnaire for general information of patients, a comprehensive Zung self-rating depression scale(SDS)and Zung self-rating anxiety scale(SAS)were used to investigate 100 patients with chronic hepatitis B patients(the study group), and the results were compared with 80 normal persons(the control group). Results The SDS and SAS score of the study group was significantly higher than the control group. The negative emotion factors of the study group included social context, family, education, self- respect. Conclusions Patients with chronic hepatitis B has obvious negative mood, and are influenced by many kinds of factors. Strengthening the comprehensive psychological intervention can improve the negative emotional state, and promote rehabilitation of patients.

14.
Article in English | WPRIM | ID: wpr-727345

ABSTRACT

Phenolic compounds affect intracellular free Ca2+ concentration ([Ca2+]i) signaling. The study examined whether the simple phenolic compound octyl gallate affects ATP-induced Ca2+ signaling in PC12 cells using fura-2-based digital Ca2+ imaging and whole-cell patch clamping. Treatment with ATP (100 micrometer) for 90 s induced increases in [Ca2+]i in PC12 cells. Pretreatment with octyl gallate (100 nM to 20 micrometer) for 10 min inhibited the ATP-induced [Ca2+]i response in a concentration-dependent manner (IC50=2.84 micrometer). Treatment with octyl gallate (3 micrometer) for 10 min significantly inhibited the ATP-induced response following the removal of extracellular Ca2+ with nominally Ca2+-free HEPES HBSS or depletion of intracellular Ca2+ stores with thapsigargin (1 micrometer). Treatment for 10 min with the L-type Ca2+ channel antagonist nimodipine (1 micrometer) significantly inhibited the ATP-induced [Ca2+]i increase, and treatment with octyl gallate further inhibited the ATP-induced response. Treatment with octyl gallate significantly inhibited the [Ca2+]i increase induced by 50 mM KCl. Pretreatment with protein kinase C inhibitors staurosporin (100 nM) and GF109203X (300 nM), or the tyrosine kinase inhibitor genistein (50 micrometer) did not significantly affect the inhibitory effects of octyl gallate on the ATP-induced response. Treatment with octyl gallate markedly inhibited the ATP-induced currents. Therefore, we conclude that octyl gallate inhibits ATP-induced [Ca2+]i increase in PC12 cells by inhibiting both non-selective P2X receptor-mediated influx of Ca2+ from extracellular space and P2Y receptor-induced release of Ca2+ from intracellular stores in protein kinase-independent manner. In addition, octyl gallate inhibits the ATP-induced Ca2+ responses by inhibiting the secondary activation of voltage-gated Ca2+ channels.


Subject(s)
Adenosine Triphosphate , Animals , Calcium , Constriction , Extracellular Space , Gallic Acid , Genistein , HEPES , Indoles , Maleimides , Nimodipine , PC12 Cells , Phenol , Protein Kinase C , Protein-Tyrosine Kinases , Thapsigargin
15.
Article in Chinese | WPRIM | ID: wpr-399779

ABSTRACT

Obiective To discuss influence of psychological intervention on compliance improvement of patients with liver cirrhosis.Methods Patients with liver cirrhosis(100 cases)were divided in to the experimental group and the control group with 50 cases in each group.The control group received routine nursing.The experimental group and their family members were given systemic psychological in tervention by responsibility nudes.The treatment effect after nursing and intervention in the two groups was compared.Results The compliance of the experimental group was better than that of the control group,P<0.05,which had statistical difference.Conclusions Implementation of psychological intervention shows significant effect on compliance improvement of patients with liver cirrhosis.

16.
Article in Chinese | WPRIM | ID: wpr-399161

ABSTRACT

Objective To summarize the nursing experiences of brucellosis to enhance nursing level of brucellosis.Methods Basic nursing,systematic nursing and mental nursing were applied to brucellosis patients,and diet instruction was conducted.Results Patients in acute phase were cured after use of antibiotics.Two cases of patients in chronic phase received evident effect after integrative medicine therapy.Conclusion Appmpriate and selective nursing can facilitate the recovery of patients with brucellosis.

17.
Article in Chinese | WPRIM | ID: wpr-586557

ABSTRACT

OBJECTIVE To investigate the status of hospital infection of elderly in internal medicine,in order to prevent and control hospital infection of them.METHODS A restrospective survey on 157 cases of hospital(infection) of elderly from Aug 2003 to Apr 2005 was conducted in internal medicine.(RESULTS)The results showed that the high risk wards were in geriatrics,neurology,endocrinology and(cardiovascular) departments.The high risk season was in winter.Respiratory tract was the most common(infective) site.Fungi were the main pathogens.CONCLUSIONS The hospital infection of elderly should be(controlled) better,geriatrics department is the key(control) unit.Winter is the key control season.The diagnostic level of clinician about the hospital(infection) of(edlerly) should be enhanced and the antibiotic must be used reasonably.

18.
Article in Chinese | WPRIM | ID: wpr-574629

ABSTRACT

Objective To establish the quality standard of Shenxin Capsules.Methods The contents of ginsenoside Rg1 and ginsenoside Re in Shenxin Capsules were determined by HPLC with the mobile phase consisting of acetonitrile- 0.05 % phosphoric acid water solution (95 ∶ 405) and UV detection wavelength at 203 nm .Qualitative analysis of Radix Ginseng, Cornu Saigae Tataricae and Herba Asari in Shenxin Capsule was carried out by TLC. Results Ginsenoside Rg1 showed a good linearity in the range of 1.032~ 9.288 ? g( r=0.9998, n=5) , the average recovery being 99.76 % and RSD being 2.10 % . Ginsenoside Re showed a good linearity in the range of 0.850~ 7.650 ? g( r=0.9998, n=5) , the average recovery being 96.24 % and RSD being 1.67 % . The chromatographic spots of Radix Ginseng, Cornu Saigae Tataricae and Herba Asari were identified without the interference of negative control .Conclusion The method is accurate , reproducible and can be used for the quality control of Shenxin Capsules.

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