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1.
China Pharmacy ; (12): 2371-2377, 2021.
Article in Chinese | WPRIM | ID: wpr-886919

ABSTRACT

OBJECTIVE:To study the sta bility,in vivo release characteristics and tissue distribution of docetaxel (DTX)- dihydroartemisinin(DHA)conjugated prodrug self-assembled nanoparticles (DTX-S-S-DHA NPs ). METHODS :HPLC method was adopted to analyze DTX-S-S-DHA in vitro . The phycial and long-term stability of DTX-S-S-DHA NPs in mediums [water , saline,phosphate buffer (PBS,pH 7.4)and RPMI 1640 medium] were investigated by using particle size ,polydispersity index (PDI)and encapsulation efficiency (EE)as evaluation indexes. The in vitro release characteristics of DTX-S-S-DHA released from DTX-S-S-DHA NPs was also investigated with small glass method ,using 30% ethanol solution with or without 10 mmol/L dithiothreitol(DTT)as medium. The small live animal imager was adopted to investigate the tissue distribution and tumor targeting capability of DiR-labeled DTX-S-S-DHA NPs (DTX-S-S-DHA/DiR NPs )in breast cancer bearing mice. RESULTS :In stability test,there was no statistical difference in particle size ,PDI and EE of DTX-S-S-DHA NPs incubated in water ,normal saline ,PBS and RPMI 1640 medium for 24 h. When stored at 4 ℃,with the increase of storage time ,the particle size of DTX-S-S-DHA NPs in normal saline gradually increased ,while those in PBS gradually decreased ;EE of both gradually decreased to less than 75%, but there was no significant change in particle size ,PDI and EE of DTX-S-S-DHA NPs in water and RPMI 1640 medium. In the in vitro release experiments ,DTX-S-S-DHA in DTX-S-S-DHA NPs was not released in the release medium containing 10 mmol/L DTT;at 24 h,the cumulative release rate of DTX-S-S-DHA released from DTX-S-S-DHA NPs in release medium without DTT was about 83%,which was in line with first-order kinetic model. In tissue distribution test ,the distribution of DTX-S-S-DHA/DiR NPs in tumor sites of mice was significantly more than in other tissues (heart,liver,spleen,lung and kidney ). CONCLUSIONS : DTX-S-S-DHA NPs show good physical stability in different mediums ,especially have good long-term stability in water and RPMI ; 1640 medium;they can quickly release the parent drug in the reduction environment and has good tumor targeting.

2.
Article in Chinese | WPRIM | ID: wpr-885943

ABSTRACT

Objective:To introduce the method and clinical effect of inferior pedicle flap used in reduction mammoplasty.Methods:From January 2010 to December 2019, 19 patients with moderate to severe macromastia who underwent reduction mammoplasty with inferior pedicle flap were enrolled in this study. The method of inferior pedicle flap was based on Robbins vertical inferior pedicle flap. New position of nipple areola and inferior pedicle flap were designed in terms of the breast anatomy.Results:A total of 19 patients (38 breasts) were included in this study. The average tissue reduction of one side breast was 570 g (385 g to 1 525 g). After operation, all patients were satisfied with natural appearance and nipple erection effect. There were no operation-related complications including nipple-areola necrosis. After 6 months to 8 years following-up, No inverted T hypertrophic scar occurred. Hyperpigmentation occurred on two patients with dark-skinned. The position of bilateral nipples and areola was higher in 1 patient after operation. The other 18 patients were satisfied with the position and shape of nipples and areola.Conclusions:It is a good choice for patients with moderate to severe macromastia to receive reduction mammoplasty with inferior pedicle flap. Through the method, large amount of breast tissue reduction and stable blood supply of nipple and areola complex could be obtained.

3.
Article in Chinese | WPRIM | ID: wpr-868581

ABSTRACT

Objective To investigate the effect and underlying mechanism of lncRNA MEG3 on the radiosensitivity of nasopharyngeal carcinoma cells.Methods this experiment,overexpression control group,MEG3 overexpression group,miR-NC inhibition group,miR-7-5p inhibition group,overexpression control+4 Gy group,MEG3 overexpression+4 Gygroup,miR-NC inhibition+4 Gy group,miR-7-5p inhibition+4 Gy group,MEG3 overexpression + miR-NC overexpression group,MEG3 overexpression + miR-7-Sp overexpression group were established.The expression of miR-7-5p and MEG3 was detected by qRT-PCR.The radiosensitivity of nasopharyngeal carcinoma cells was measured by clone formation assay.Cell apoptosis was assessed by flow cytometry.The fluorescence activity was evaluated by dual luciferase reporter assay.Results MEG3 was lowly expressed in nasopharyngeal carcinoma tissues and cells.Overexpression of MEG3 and inhibition of miR-7-5p expression increased the radiosensitivity of nasopharyngeal carcinoma cells and promoted radiation-induced cell apoptosis.MEG3 could targetedly regulate the miR-7-5p expression.Overexpression of miR-7-5p reversed the effect of overexpression of MEG3 on the sensitization of nasopharyngeal carcinoma cells and the promotion of apoptosis induced by radiation exposure.Conclusions Overexpression of MEG3 increases the radiosensitivity of nasopharyngeal carcinoma cells and promotes radiation-induced cell apoptosis.The mechanism may be related to the down-regulation of miR-7-5p expression.

4.
Article in Chinese | WPRIM | ID: wpr-863008

ABSTRACT

Tenascin-C(TN-C) is an extracelluar matrix glycoprotein, which is transiently expressed during the early stage of embryonic development and widely involved in cell proliferation and differentiation, tissue repair and so on.TN-C expression is modulated by mechanical stress, multiple signaling pathways and transcription factors.By inducing the proliferation of vascular smooth muscle cells and mediating infiltration of inflammatory cells, TN-C promotes the onset and development of cardiovascular diseases, and is significantly associated with the activation, risk stratification and disease prognosis.This review summarizes the characteristics of expression pattern of tenascin-C in the development of cardiovascular system, such as heart, aorta, and coronary artery, and its application in the clinical diagnosis, intervention and prognostic prediction of pediatric acute myocarditis, dilated cardiomyopathy, rheumatic heart disease, Marfan syndrome, Kawasaki disease and pulmonary hypertension.

5.
Article in Chinese | WPRIM | ID: wpr-870191

ABSTRACT

In recent years, the clinical guidelines for the diagnosis and treatment of rheumatoid arthritis (RA) have been constantly updated. Among the general principles, it is particularly emphasized that, in order to improve the ratio of treat to target(T2T) of RA, doctors and patients should work together to negotiate the details of the guidelines. Therefore, it is important for patients to further understand the disease and clinical guidelines of RA, and to better cooperate with doctors. This study was based on the most concerned issues of RA patients and international standard procedure of guideline study, we organized the working group and introduce the following 16 recommendations constituting the RA patients′ practice guidelines.

6.
Chinese Journal of School Health ; (12): 1536-1539, 2020.
Article in Chinese | WPRIM | ID: wpr-829322

ABSTRACT

Objective@#To understand physical activity status of students in Zhengzhou vocational colleges and its relationship with sleep and mental health, and to provide support for the development of physical and mental health and intervention.@*Methods@#A total of 6 224 college students were randomly selected from freshmen and sophomores in 4 vocational colleges in Zhengzhou and were investigated with questionnaire regarding physical activity, sleep quality, and mental health.@*Results@#The sedentary time and middle-high intensity physical activity time of college students in Zhengzhou vocational colleges were (91.23±56.25)(56.25±40.21)min/d; anxiety and sleep scores were (37.41±7.72)(7.91±1.77) points. The proportion of students meeting the gjideline of physical activity and senentary time was 22.27% and 51.83%, respectively, the prevalence of anxiety was 9.22%, and the detection rate of poor sleep was 52.81%. The percentage of female students who met the guideline for static behavior, reporting anxiety and poor sleep was higher than that of male students, and the difference was statistically significant(χ2=7.16, 5.93, 39.34, P<0.05). The scores of anxiety and sleep quality of the physically qualified boys were lower than those of the unqualified boys, and the differences were statistically significant(t=-10.33, -11.25, P<0.01). Logistic regression analysis showed that compared with those who were qualified for physical activity and static behavior, the risk of anxiety and sleep disturbance was 2.45 and 1.89 times for the unqualified students, and 2.24 and 1.49 times after adjustment(P<0.01).@*Conclusion@#Students in Zhengzhou vocational colleges are lack of physical activity and have long sedentary time, which might be associated with anxiety and sleep quality problems. Physical and mental health among college students could be improved through physical activity promotion.

7.
Chinese Journal of Plastic Surgery ; (6): 1209-1212, 2019.
Article in Chinese | WPRIM | ID: wpr-800209

ABSTRACT

Objective@#To explore procedures and clinical effect of the technique, combined reposition of rectus abdominis with abdminioplasty, for treating postpartum women with diastasis rectus abdominis and lax abdominal wall.@*Methods@#Postpartum women with diastasis rectus abdominis and lax abdominal wall who underwent reposition of rectus abdominis and abdminioplasty were enrolled in this study. This combined technique was based on abdominoplasty, including reposition of rectus abdominis, umbilical transposition and tightening abdominal wall.@*Results@#A total of 12 patients were included in this study. Umbilical transposition was performed in 9 patients. Skin was resected by 8 to 14 centimetre in width.There were no infection, skin necrosis, and delayed wound healing.Natural abdominal contour has been found in all patients and the abdominal perimeter as well as the waistline have shrunk.Further more, this combined technique has been shown to improve quality of life by reducing pain, restoring function, and increasing satisfaction with appearance.After 3 months to 2 years following up, all patients were satisfied with operative outcomes.@*Conclusions@#This combined technique has been shown to be a good choice for postpartum women with diastasis rectus abdominis and lax abdominal wall.

8.
Chinese Journal of Stomatology ; (12): 469-474, 2019.
Article in Chinese | WPRIM | ID: wpr-810697

ABSTRACT

Objective@#To compare the effect on the flow conditions of adhesives and the retention force of restorations among different cement-retained methods of implant-supported fixed prostheses.@*Methods@#Four common cement-retained methods were selected, including the occlusal hole for screw access (OH), the lingual hole for adhesives overflow (LH), the resin replica for titanium abutment (RR), and the traditional cement-retained method (the control group). The adhesive used in this study was resin-modified glass ionomer cement. The two-dimensional analysis models of computational fluid dynamics (CFD) were established. The flow conditions of adhesives in the adhesion process was analyzed by the CFD analysis. The internal filling ratio and the amount of neck overflow of adhesives below the edge of the prosthesis were calculated. Ten zirconia prostheses in each group were processed and cemented. The retention force was examined by mechanical tensile experiments in vitro.@*Results@#The CFD analysis showed the internal filling ratio of adhesives from high to low was the LH group, the OH group, the RR group and the control group. The amount of neck overflow of adhesives below the edge of the prosthesis from less to more was the RR group, the OH group, the LH group and the control group. The retention force was (240.7±33.9) N in the control group, (278.2±59.1) N in the OH group, (292.9±47.9) N in the LH group, and (262.8±59.4) N in the RR group. There was a statistically significant difference in the retention force between the LH group and the control group (P=0.029). There was no significant difference among the other groups (P>0.05).@*Conclusions@#The modified cement-retained methods can effectively reduce the amount of neck overflow of adhesives, and improve the filling condition of adhesive in the adhesive clearance to ensure the retention force of the prostheses. Clinically, the appropriate modified cement-retained method should be selected according to the three-dimensional position of the implant and the position of prosthetic margin.

9.
Article in Chinese | WPRIM | ID: wpr-703283

ABSTRACT

The proliferation of tumor cells is regulated by a complex array of signaling pathways, among these signaling pathways,the programmed cell death. Autophagy and apoptosis are two types of programmed death. There are significant differences in their morphological and functional features, but they also have many links. Both apoptosis and autophagy are involved in activation,expression and regulation of a series of genes. By reviewing the research progress in recent years, this article will discuss the cellular regulation and molecular mechanisms of their related genes. Through summarizing the relationship between autophagy and apoptosis,it aims to get a better understanding of the mechanisms of autophagy and apoptosis in tumor progression,and looking at the perspectives for studies on the autophagy and apoptosis in tumor treatment.

10.
Article in Chinese | WPRIM | ID: wpr-703222

ABSTRACT

Objective To explore the biological indicators of diagnosis and treatment of irritable bowel syndrome (IBS), and to explore the mechanism of action of a Chinese medicine Wuji Pill (WJW) on irritable bowel syndrome (IBS). Methods (1) Postinflammatory irritable bowel syndrome (PI-IBS) rat model was established by acetic acid plus restraint stress method . (2) The colonic motor ability of rats was evaluated by colon motility index (MI), the number of fecal particles discharged within 2 h, and the time of glass pellet discharge. (3) The formation of PI-IBS model rats and the therapeutic effect of WJW were observed. (4) The levels of calcitonin gene-related peptide (CGRP), motilin (MTL), neuropeptide Y (NPY), substance P (SP), somatostatin (SS), vasoactive intestinal peptide (VIP), and cholecystokinin (CCK) in the brain and colon tissues of PI-IBS rats were measured by ELISA. Results (1) The rat PI-IBS model was successfully established. Compared with the normal group, the body weight of the model rats was decreased, the food intake decreased, the amount of feces increased, loose stools and amorphous soft stools appeared, voluntary movements decreased, colon motility index ( MI) significantly increased ( P < 0. 05 ), the number of fecal particles discharged significantly increased ( P< 0. 05), and the glass pellet discharge time was significantly shortened ( P < 0. 05). (2) WJW treatment for 7 days significantly improved a variety of symptoms. Compared with the normal control, the levels of CGRP, SS and VIP in the brain tissue of PI-IBS rats were significantly increased (P< 0. 05), and the NPY concentration was significantly decreased ( P < 0. 05). However, the treatment with WJW significantly reduced CGRP, SS and VIP levels (P< 0. 05), and significantly increased the NPY concentration level (P < 0. 05). (3) Compared with the normal control group, the levels of CCK, NPY, MTL, SS and VIP in colonic tissues of PI-IBS rats were significantly decreased (P< 0. 05), while WJW significantly increased the CCK and VIP levels. Conclusions WJW can be used to treat IBS by regulating the levels of various brain-gut peptides in the brain and colon tissues of IBS rats. These anomalous and adjustable brain-gut peptides may become a potential biomarker for the diagnosis and treatment of IBS.

11.
Article in Chinese | WPRIM | ID: wpr-699696

ABSTRACT

Objective To investigate the neuroprotective effect and antioxidation mechanism of tianmagouteng decoction on retinal ganglion cells (RGCs) in optic nerve crush model.Methods Optic nerve crush models were established using optic nerve clamping method in the right eyes of 50 SPF male Wistar rats.The rats were randomized into model group (17 rats),1.2 g/ml tianmagouteng decoction group (16 rats) and 2.4 g/ml tianmagouteng decoction group (17 rats).Ttianmagouteng decoction at the dose of 1.2 g/(ml· d) or 2.4 g/(ml · d) was intragastrically administered 2 hours after onset of optic nerve damage (10 ml/kg),respectively,based on grouping for consecutive 28 days,and equal volume of distilled water was used in the same way in the model group.Fluoro-gold (FG,3 μl,3%) was bilaterally injected into superior colliculus to retrogradely label RGCs 23 days following modeling.The rats were sacrificed at day 28 and retinal flatmounts were prepared to count RGCs.Retinal glutathione peroxidase (GSH-Px) activity in rat retinas was detected by chemical colorimetric analysis.Results The survival rate of RGCs was (59.67 ± 9.85) %,(71.33 ± 9.14) % and (73.63 ± 8.33) % in the model group,1.2 g/ml tianmagouteng decoction group and 2.4 g/ml tianmagouteng decoction group,respectively,showing a significant difference among the three groups (F =5.322,P =0.014),and the survival rate of RGCs was evidently higher in the 1.2 g/ml tianmagouteng decoction group and 2.4 g/ml tianmagouteng decoction group than that in the model group (P =0.023,0.006).The GSH-Px activity was (222.20±76.67),(311.30 ±46.93) and (473.65 ± 117.73)μmol/(s · mg) in the model group,1.2 g/ml tianmagouteng decoction group and 2.4 g/ml tianmagouteng decoction group,respectively,with a significant difference among the three groups (F =20.005,P < 0.001),and the GSH-Px activity in the 2.4 g/ml tianmagouteng decoction group is considerably increased as compared to the model group and 1.2 g/ml tianmagouteng decoction group (P < 0.001;P =0.001).Conclusions Tianmagouteng decoction plays a neuroprotective effect on RGCs after optic nerve damage in rat,which may be achieved by improving the activity of the GSH-Px in retina,suggesting that antioxidation probably be one of the neuroprotective mechanisms of tianmagouteng decoction.

12.
Article in Chinese | WPRIM | ID: wpr-610383

ABSTRACT

Neurodegenerative diseases are threating our health seriously.Inflammation plays an important role in the initiation and development of neurodegenerative diseases, and its primary characteristics are the activation of microglia and the increasing level of inflammation cytokines.This review describes the relationship between neuroinflammation and several neurodegenerative diseases, and the models in vivo and in vitro.In addition, combining with traditional Chinese medicines knowledge of encephalopathy, we summarizes pharmacological effects and mechanisms of multiple herb extracts and monomer compounds in preventing the activation of microglia and inhibiting neuroinflammation, thus, to provide the basis for gradually revealing the related rules and characteristics of treating encephalopathy by traditional Chinese medicine, and improving the accuracy of the clinical drugs, as well as developing new drugs for the prevention and control of encephalopathy.

13.
Herald of Medicine ; (12): 370-374, 2017.
Article in Chinese | WPRIM | ID: wpr-609601

ABSTRACT

Objective To investigate the effects of Siwu decoction on the learning and memory abilities in mice with blood deficiency and the related mechanism.Methods The mice were randomly divided into five groups:normal control group,model control group,high-,medium-,and low-dose group of Siwu decoction.Blood deficiency mouse model was established by continuously cuttingtailand bleedingin model control group,high-,medium-,and low-dose group of Siwu decoction.The mice in high-,medium-,and low-dose group were intragastrically administrated with Siwu decoction of 2.5,5.0,10.0 g·kg-1 for 14 days,respectively.The erythrocyte counts (RBC),hemoglobin (HGB),red blood cell hematocrit (HCT) were detected before modeling,7 and 14 days after administration.The spatial learning and memory abilities were assessed using the T maze test.The spontaneous activities were assessed using locomotors activity detector.The levels of erythropoietin (EPO) content in serum and acetylcholinesterase (AChE) in hippocampal tissue were detected by enzyme-linked immunousorbent assay.Results 5.0 and 10.0 g·kg-1Siwu decoction could increase RBC,HGB,HCT on the 7th and 14th day,2.5 g.kg-1Siwu decoction could increase RBC,HCT on 14th day,and each dosage of Siwu decoction could significantly increase the spontaneous activities of model mice with blood deficiency on the 7th and 14th day as compared with model control group (P <0.05 or P <0.01).Compared with model control group,the number of errorsin T Maze test were decreased significantly in high-,medium-,and low-dose groups (P < 0.01).And the Siwu decoction had a tendency to reduce the hippocampal AChE levels,but when compared with the model control group there were no statistical differences (P > 0.05).Conclusion Siwu decoction is benefit formemory and spatial learning in mice with blood deficiency,which may be related with higher serun EPO and lower hippocampal AChE expression.

14.
Chinese Journal of Endemiology ; (12): 675-678, 2017.
Article in Chinese | WPRIM | ID: wpr-662632

ABSTRACT

Objective To analyze the risk factors of thyroid nodule (TN) in Harbin City,Heilongjiang Province.Methods From November 2014 to January 2015,residents lived in Nangang,Daoli,Daowai districts and villagers of Xubao Town of Hulan District of Harbin City were selected as research subjects,who lived in the local regions for more than 5 years,a total of 2 650 people were surveyed.Influence factors of TN were surveyed by general survey,clinical examination and thyroid ultrasound examination.Logistic regression analysis was used to analyze relationships between various factors and TN in single factor analysis and multiple factors analysis.Results Excluding 36 cases with thyroid surgery history,the total prevalence of TN was 22.92% (599/2 614),single factor Logistic regression analysis found that females [odds ratio (OR) =1.597,95% confidence interval (CI) =1.328-1.919],elderly (OR =3.356,95%CI =2.699-4.173),living in cities and towns (OR =1.677,95%CI =1.391-2.021),obesity (OR =1.245,95%CI =1.036-1.497),history of hypertension (OR =1.766,95%CI =1.414-2.205) were the risk factors of TN.In multiple factors Logistic regression analysis,risk factors from high to low in the order were elderly (OR =3.076,95%CI =2.436-3.884),females (OR =1.819,95%CI =1.493-2.215),living in cities and towns (OR =1.701,95%CI =1.400-2.066),history of diabetes (OR =1.644,95%CI =1.235-2.189),obesity (OR =1.325,95%CI =1.085-1.618).Conclusions The risk factors of TN include living in cities and towns,females,elderly,obesity,history of hypertension or diabetes in Harbin City of Heilongjiang Province.

15.
Chinese Journal of Endemiology ; (12): 675-678, 2017.
Article in Chinese | WPRIM | ID: wpr-660450

ABSTRACT

Objective To analyze the risk factors of thyroid nodule (TN) in Harbin City,Heilongjiang Province.Methods From November 2014 to January 2015,residents lived in Nangang,Daoli,Daowai districts and villagers of Xubao Town of Hulan District of Harbin City were selected as research subjects,who lived in the local regions for more than 5 years,a total of 2 650 people were surveyed.Influence factors of TN were surveyed by general survey,clinical examination and thyroid ultrasound examination.Logistic regression analysis was used to analyze relationships between various factors and TN in single factor analysis and multiple factors analysis.Results Excluding 36 cases with thyroid surgery history,the total prevalence of TN was 22.92% (599/2 614),single factor Logistic regression analysis found that females [odds ratio (OR) =1.597,95% confidence interval (CI) =1.328-1.919],elderly (OR =3.356,95%CI =2.699-4.173),living in cities and towns (OR =1.677,95%CI =1.391-2.021),obesity (OR =1.245,95%CI =1.036-1.497),history of hypertension (OR =1.766,95%CI =1.414-2.205) were the risk factors of TN.In multiple factors Logistic regression analysis,risk factors from high to low in the order were elderly (OR =3.076,95%CI =2.436-3.884),females (OR =1.819,95%CI =1.493-2.215),living in cities and towns (OR =1.701,95%CI =1.400-2.066),history of diabetes (OR =1.644,95%CI =1.235-2.189),obesity (OR =1.325,95%CI =1.085-1.618).Conclusions The risk factors of TN include living in cities and towns,females,elderly,obesity,history of hypertension or diabetes in Harbin City of Heilongjiang Province.

16.
The Journal of Practical Medicine ; (24): 2213-2216, 2017.
Article in Chinese | WPRIM | ID: wpr-617105

ABSTRACT

Objective To assess the diagnostic value of combining aortic dissection detection risk score (ADDRS)with D-dimer in patients with suspected acute aortic dissection(AAD). Methods We retrospectively identified 525 patients with chest pain and with calculated ADDRS and available D-dimer on admission between January 2015 and December 2016. ADDRS ≤1 was defined as ′low probability′ and that >1 as ′high probability′. Results AAD were diagnosed in 106(20.2%)patients. In patients with ADDRS of 0,1 case of AAD was identi-fied and in those with ADDRS of 1,40 were identified while in 76 patients with ADDRS of 2 and 3,71 cases of AAD were identified. D-dimer with threshold of 0.5 mg/L had a negative predictive value of 100%and a specificity of 76.6% in patients with ADDRS of 0. In patients with ADDRS of 1,D-dimer had a negative predictive value of 98.6%. D-dimer showed a negative predictive value of 99.6% and a positive predictive value of 22.3% in patients with low probability. Conclusions High ADDRS benefits confirming AAD. Low ADDRS combined with negative D-dimer is safe and efficient for ruling out AAD ,while low ADDRS with positive D-dimer needs further aortic imaging.

17.
Progress in Modern Biomedicine ; (24): 4572-4575, 2017.
Article in Chinese | WPRIM | ID: wpr-614846

ABSTRACT

Objective:To investigate the clinical effect of endoscopic sinus surgery in the treatment of sinusitis and nasal polyps and its effect on nasal ventilation and olfactory function.Methods:80 patients with sinusitis and nasal polyps were selected in our hospital from January 2014 to June 2016,who were randomly divided into observation group and control group,each group of 40 cases.The control group was treated with traditional enucleation,while the observation group was treated with endoscopic sinus surgery.The clinical efficacy,the symptom scores,nasal airway resistance and olfactory function score before treatment and 3 months after treatment were compared between the two groups.Results:The total effective rate of the observation group was 95.00%,which was significantly higher than that of the control group 77.50% (P<0.05).There was no significant difference in nasal congestion,purulent nasal discharge,olfactory dysfunction,pain and total symptom score between the two groups before treatment (P>0.05),nasal congestion,purulent nasal discharge,olfactory dysfunction,pain and total symptom score between the two groups at 3 months after treatment were lower than before treatment,and nasal pus,smell disorder,pain and total symptom scores of the patients in the observation group were lower than the control group (P<0.05).There was no significant difference in nasal airway resistance and olfactory function score between the two groups before treatment and 3 months after treatment (P>0.05),nasal airway resistance,olfactory function score of patients in two groups at 3 months after treatment were lower than before treatment,and the observation group was lower than that ofthe control group (P<0.05).Conclusion:Endoscopic sinus surgery for sinusitis and nasal polyps is helpful to improve the clinical symptoms,promote the olfactory function and recovery of nasal ventilation,which is an effective method for treatment of sinusitis and nasal polyps.

18.
Article in Chinese | WPRIM | ID: wpr-668786

ABSTRACT

Objective To investigate the risk factors of surgical site infection in patients with colorectal cancer.Methods Clinical data of 542 patients with colorectal cancer were retrospectively studied.According to the infection of surgical site or not,all patients were assigned into infection group (n =48) or non-infection group (n =494).The risk factors of surgical site infection were analyzed.Results Compared with non-infection group,the patients in the infection group got significant increase in age [(67.74 ± 9.46) years vs.(57.63 ± 9.94) years,t =7.593,P =0.000],significantly higher rate of diabetes (25.00% vs.8.70%,x2 =12.843,P =0.000),significantly x higher rate of open surgery (70.83% vs.33.81%,x2 =25.946,P =0.000),and significantly longer operation duration [(123.45 ± 23.56) min vs.(110.47 ± 19.47) min,t =10.485,P =0.000].Logistic study showed that age,diabetes,open surgery and operation duration were risk factors for the development of surgical site infection (all P < 0.05).Conclusion Age,diabetes,open surgery and operation duration are risk factors for the development of surgical site infection in colorectal cancer.

19.
Article in Chinese | WPRIM | ID: wpr-666795

ABSTRACT

Objective To evaluate the role of NOX2 in bupivacaine-induced production of reactive oxygen species (ROS) in nerve cells.Methods SH-SY5Y cells were seeded in culture plates and divided into 4 groups (n =11 each) using a random number table:small interfering RNA (siRNA) negative control group (group NC),siRNA negative control plus bupivacaine group (group NC +B),NOX2 siRNA group and NOX2 siRNA plus bupivacaine group (group NOX2 siRNA + B).In NC and NOX2 siRNA groups,the cells were transfected with negative siRNA and NOX2 siRNA,respectively,and then incubated in the culture medium for 24 h.In NC+B and NOX2 siRNA+B groups,cells were transfected with negative siRNA and NOX2 siRNA,respectively,new plates were used,the cells were incubated for 3 h with bupivacaine at the final concentration of 1.5 mmol/L,the culture medium was then replaced,and the cells were incubated until 24 h.The level of intracellular ROS was measured using the fluorogenic probe dihydroethidium,the cell apoptosis was determined by TUNEL,and the expression of activated caspase-3 and caspase-9 was detected using Western blot.Apoptosis rate was calculated.Results Compared with group NC,the level of ROS and apoptosis rate were significantly increased,and the expression of activated caspase-3 and caspase-9 was up-regulated in group NC+B (P< 0.05),the level of ROS was significantly increased,and the expression of activated caspase-3 and caspase-9 was up-regulated (P<0.05),and no significant change was found in apoptosis rate in group NOX2 siRNA+B (P>0.05),and no significant change was found in the level of ROS or apoptosis rate (P>0.05),and the expression of activated caspase-3 and caspase-9 was significantly up-regulated in group NOX2 siRNA (P< 0.05).Compared with group NC+B,the level of ROS and apoptosis rate were significantly decreased,and the expression of activated caspase-3 and caspase-9 was down-regulated in group NOX2 siRNA+B (P<0.05).Conclusion NOX2 is involved in the pathophysiological mechanism of bupivacaine-induced burst production of ROS in nerve cells.

20.
Article in Chinese | WPRIM | ID: wpr-345350

ABSTRACT

<p><b>OBJECTIVE</b>To report on a novel HLA-A allele, A*29:49, identified in a Chinese Han population by sequence based typing (SBT).</p><p><b>METHODS</b>A donor from China Marrow Donor Programme (CMDP) was typed with a bi-allelic PCR-SBT kit, and no full matched result was obtained for the HLA-A locus. The novel HLA allele was verified with an allele-specific amplification SBT kit.</p><p><b>RESULTS</b>A novel HLA-A allele was identified, which has differed by one nucleotide from the closest matched allele, HLA-A*29:01:01:01, at position 368(A→T), codon 99 (TAT→TTT), resulting in an amino acid substitution (Y→F). Another allele was verified as A*02:06:01.</p><p><b>CONCLUSION</b>A novel HLA-A allele was identified and officially named as HLA-A*29:49 by the WHO Nomenclature Committee for Factors of the HLA System.</p>


Subject(s)
Alleles , Amino Acid Substitution , Genetics , Base Sequence , China , HLA-A Antigens , Genetics , Humans , Sequence Analysis, DNA , Methods
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