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1.
Article in Chinese | WPRIM | ID: wpr-905279

ABSTRACT

Objective:To explore the effects of respiratory training on pulmonary and motor function for patients with Parkinson's disease. Methods:From January, 2018 to November, 2019, 60 inpatients with idiopathic Parkinson's disease from the Second Rehabilitation Hospital of Shanghai were randomly divided into control group (n = 30) and experimental group (n = 30). All the patients accepted routine rehabilitation, while the experimental group accepted respiratory training with Power Breathe in addition. They were measured the pulmonary function, and assessed with Unified Parkinson's Disease Rating Scale (UPDRS) part II and III, and modified Barthel Index (MBI) before and four weeks after treatment. Results:The scores of UPDRS II and III, and MBI improved in both groups after treatment (|t| > 2.550, P < 0.05), while the forced vital capacity (FVC), forced expiratory volume in the first second (FEV1) and maximum expiratory flow rate at 50% vital capacity (MEF50) increased in the experimental group (|t| > 2.838, P < 0.01), but did not in the control group (|t| < 1.058, P > 0.05). FVC, FEV1, MEF50, MBI score and UPDRS II score improved more in the experimental group than in the control group (|t| > 2.191, P < 0.05). Conclusion:Respiratory training can improve pulmonary function for patients with Parkinson's disease, to further improve their activities of daily living. No synergistic effect is found on motor function.

2.
Journal of Experimental Hematology ; (6): 1471-1477, 2021.
Article in Chinese | WPRIM | ID: wpr-922281

ABSTRACT

OBJECTIVE@#To investigate the optimal time of monitoring minimal residual disease (MRD) for predicting survival and prognosis in children with T-cell acute lymphoblastic leukemia (T-ALL) after treated by CCLG-ALL2008 chemotherapy.@*METHODS@#96 children with T-ALL receiving CCLG-ALL2008 chemotherapy treated in our hospital from January 2015 to January 2020 were retrospectively summarized. The follow-up time was 9.0-65.0 months, with a median of 43.5 months. Kaplan-Meier survival curve was used to detect the overall event-free survival (EFS) and overall survival (OS) of the patients. The clinical data, MRD levels after 15 d, 33 d and 90 d chemotherapy between EFS group and relapse group, as well as OS group and death group were compared by using univariate analysis. Multivariate Logistic regression analysis was used to screen the main risk factors affecting EFS and OS of the patients. The patients were divided into low, moderate and high-risk according to the MRD level after 15 d, 33 d and 90 d, the differences of EFS and OS between each groups were compared again.@*RESULTS@#By the end of follow-up, 50 patients recurred and other 46 patients non-recurred; 40 patients died and 56 patients survived, the EFS was (49.5±6.3)% and OS was (61.5±5.9)%. Univariate analysis showed that the initial WBC count in EFS group (n=46) was significantly lower than that in relapse group (n=50), and MRD levels after 33 d and 90 d were significantly less also (P0.05), however for 90 d, EFS and OS of the patients in high-risk group were significantly lower than those in medium-risk group, and those in medium-risk group were lower than those in low-risk group (P<0.05).@*CONCLUSION@#The MRD level after 90 days CCLG-ALL2008 chemotherapy may be the best time to predict the survival and prognosis in T-ALL children.


Subject(s)
Child , Disease-Free Survival , Humans , Neoplasm, Residual , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Precursor T-Cell Lymphoblastic Leukemia-Lymphoma , Prognosis , Retrospective Studies , Risk Factors , T-Lymphocytes
3.
Article in Chinese | WPRIM | ID: wpr-873282

ABSTRACT

Objective::To investigate the protective effect of salvianolic acid B on HepaRG hepatocyte injury induced by arsenic trioxide (As2O3 ) and its mechanism. Method::HepaRG cells were incubated with 5μmol·L-1 As2O3 for 24 h to induce hepatocyte injury. The cells were divided into control group, model group, salvianolic acid B 10 μmol·L-1 group, salvianolic acid B 10 μmol·L-1+ As2O3 group, salvianolic acid B 5 μmol·L-1+ As2O3 group, and salvianolic acid B 2.5 μmol·L-1+ As2O3 group. HepaRG cells were preincubated with salvianolic acid B for 2 h and then incubated with As2O3 for 24 h. At the end of the incubation, cell viability was detected by thiazolyl blue tetrazolium bromide assay, apoptosis was observed by Hoechst33342 fluorescence staining, apoptosis rate was detected by annexin V-FITC/propidium iodide double staining flow cytometry, and mitochondrial membrane was observed by JC-1 fluorescence staining. Western blot was used to detect the protective effect of expressions of relevant proteins Bcl-2, Bax, Akt, p-Akt on salvianolic acid B in the liver. Result::As2O3 concentration-dependently reduced the survival rate of HepaRG cells(P<0.01), salvianolic acid B had no effect on normal cell viability for 2 h, pre-incubation with salvianolic acid B(5, 10 μmol·L-1) for 2 h significantly increased the decreased cell survival rate caused by As2O3 (P<0.01). As2O3 significantly increased hepatocytes apoptosis rate(P<0.01), while pre-incubation with salvianolic acid B(10 μmol·L-1) deceased apoptosis rate(P<0.01). Incubation with As2O3 for 24 h caused decrease of mitochondrial membrane potential, pre-incubation with salvianolic acid B maintained mitochondrial membrane potential, indicating that the anti-apoptotic effect of salvianolic acid B were related to the mitochondrial pathway modulation. Western blot analysis showed that salvianolic acid B promoted the ratio of Bcl-2/Bax and promoted p-Akt/Akt compared with As2O3 group(P<0.01). Conclusion::Salvianolic acid B has a protective effect on hepatocyte injury induced by As2O3, and its mechanism is related to maintenance of mitochondrial function and inhibition of hepatocyte apoptosis.

4.
Article in Chinese | WPRIM | ID: wpr-872746

ABSTRACT

Scutellarin is a flavonoid extracted from breviscapus, a traditional Chinese medicine. Pharmacological studies have shown that scutellarin has anti-inflammatory, antioxidant, anti-fibrosis, anti-tumor, improving cardiac and cerebral ischemia. In recent years, with the deepening of research on scutellarin, it was found that it could inhibit the tumor through multi-target and multi-pathway, and the anti-human colorectal cancer was related to the regulation of p53 pathway, Hedgelog pathway and erythropoietin generates liver cancer interactivator B2(EphrinB2).The anti-esophageal squamous cell carcinoma is related to protein kinaseB1 /protein kinaseB2( Akt1/Akt2).Anti-renal carcinoma and melanoma are associated with phosphatase and tension protein homologues(PTEN) and phosphatidylinositol 3-kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR) pathway. Anti-lung cancer is related to Akt/mTOR/4E binding protein1(4EBP1) and signal transduction and transcriptional activator(STAT3 )signaling pathway. Anti-cervical cancer is related to pyruvate kinase 2(PKM2).Anti-breast cancer is associated with Hippo/YAP pathway. At the same time, scutellarin was found to prevent diabetic microangiopathy, atherosclerosis and non-alcoholic fatty liver disease by regulating glucose and lipid metabolism, but the mechanism of action was not well studied. A review of the literature found that scutellarin anti-tumor, atherosclerosis, diabetic microangiopathy, non-alcoholic fatty liver disease, osteoarthritis, osteoporosis mechanism of action lack of detailed summary. In this paper, the research progress of pharmacological action and mechanism of scutellarin in recent 5 years is reviewed, and Suggestions on its current research status and future direction are put forward, in order to speed up the discovery of pharmacological mechanism of scutellarin and provide scientific basis for its further development and utilization.

5.
Article in Chinese | WPRIM | ID: wpr-870639

ABSTRACT

Ten patients with allergic granulomatosis with polyangiitis (AGPA) were admitted in Nanjing Drum Tower Hospital during January 2013 to August 2019, among whom 5 cases with neurologic involvement. The clinical features, laboratory findings and clinical outcome of the 5 patients were analyzed and literature review was performed. Among 5 cases of AGPA with neurologic involvement, 3 presented with peripheral neuropathy as the initial symptom, 2 had multiple mononeuropathy, 3 had distal asymmetric or symmetric polyneuropathy. All five patients had acute or subacute onset, and the symptoms of limb numbness or pain were prominent. Electrophysiological examination showed that sensory and motor conduction amplitude significantly decreased or disappeared. Eight of the 10 AGPA patients were treated with corticosteroid combined with immunosuppressants, 2 were treated with corticosteroid alone. Eight patients had good prognosis and 2 patients died. The results suggest that peripheral neuropathy is common in AGPA. When the patients present with acute or subacute onset of axonal impairment of peripheral neuropathy and elevated eosinophils, AGPA should be considered.

6.
Chinese Journal of Geriatrics ; (12): 155-158, 2020.
Article in Chinese | WPRIM | ID: wpr-869343

ABSTRACT

Objective:To investigate whether the basal blood glucose level before cerebral infarction has an impact on acute stress hyperglycemia.Methods:A total of 366 patients with cerebral infarction admitted into the neurology department of our hospital from February 2008 to May 2012 were enrolled in this study. Acute stress hyperglycemia was reflected by stress-induced glucose up-regulation ratio (SIGUR), and the basal glucose before cerebral infarction was reflected by glycosylated hemoglobin A1c (HbA1c). The correlation between SIGUR and HbA1c was analyzed in the different populations. The difference in SIGUR was compared among the non-diabetic group, diabetes with poor blood glucose control group (HbA1≥6.5%) and diabetes with well blood glucose control group (HbA1c<6.5%). The relationship between high level of SIGUR (Q4) and HbA1c was performed through logistic regression analysis.Results:SIGUR was correlated with HbA1c, no matter in the non-diabetic, diabetic or total populations ( r=-0.200, 0.195 and 0.324, P=0.010, 0.011 and 0.000). The level of SIGUR was higher in diabetes with poor blood glucose control group than in the non-diabetes and diabetes with well blood glucose control group ( F=25.842, P=0.000), but there was no significant difference between the non-diabetic group and diabetes with well blood glucose control group ( P>0.05). Logistic regression analysis showed that the high level of SIGUR was correlated to HbA1c in the total populations ( OR=1.460, P=0.000). In the diabetic group, the probability of higher SIGUR level was increased along with the increased HbA1c level ( OR=1.237, P=0.021), while the probability of higher SIGUR level was decreased along with the increased HbA1c level in the non-diabetic group ( OR=0.233, P=0.010). Conclusions:Acute stress hyperglycemia is correlated to the basal blood glucose before cerebral infarction, and blood glucose increases more prominently in those patients with high basal blood glucose level, especially in the diabetic patients.

7.
Chinese Journal of School Health ; (12): 583-587, 2020.
Article in Chinese | WPRIM | ID: wpr-821433

ABSTRACT

Objective@#To investigate the associated factors of myopia among primary and secondary school students in Shenzhen, and to provide reference for the prevention and control of myopia.@*Methods@#By stratified cluster sampling, 3 073 students of 14 schools including primary,junior,regular and vocational senior schools from two districts in Shenzhen were selected and investigated.@*Results@#For primary school students, the time of using computer for 2-<3 hours per day (OR=2.23,95%CI=1.19-4.20) , and no physical education class(2 sections per week OR=0.34, 95%CI=0.13-0.91; 4 sections per week OR=0.23, 95%CI=0.08-0.62; 5 sections or more per week OR=0.33, 95%CI=0.11-0.97) were positively associated with myopia. Teachers finishing class on time at break (occasionally delaying OR=1.99, 95%CI=1.51-2.63; frequently delaying OR=2.07, 95%CI=1.29-3.30), taking 0.5-1 hour break when using eyes at close range (1-<2 hours OR=1.33,95%CI=1.03-1.70; ≥3 hours OR=1.87, 95%CI=1.17-3.00), no parents with myopia(one parent with myopia OR=1.69, 95%CI=1.32-2.17; two parents with myopia OR=2.13, 95%CI=1.50-3.02) were negatively associated with myopia. For junior high school students, without parents with myopia (one parent with myopia OR=3.27, 95%CI=2.17-4.94; two parents with myopia OR=5.38, 95%CI=2.78-10.42) was the protective factor of myopia. For senior high school students, male (female OR=1.52, 95%CI=1.07-2.14), doing eye exercises twice a day in school (OR=0.41, 95%CI=0.23-0.75), and accumulating outdoor activities for ≥2 hours a day (OR=0.70, 95%CI=0.49-1.00) were negatively associated with myopia.@*Conclusion@#There are different risk factors for myopia among different students in Shenzhen. Students with high risk factors are the key objects of prevention and control.

8.
Article in Chinese | WPRIM | ID: wpr-851341

ABSTRACT

Objective: To research the effects of total flavonoids of Livistona chinensis (TFFL) on normal liver LO2 cells induced by acetaminophen (APAP), and study the liver protective mechanism on liver injury induced by acetaminophen in vitro. Methods: The effects of TFFL on LO2 cells and cell activity induced by acetaminophen were determined by MTT method; The apoptosis rate of LO2 cells induced by APAP was researched by Flow Cytometry combine with staining agent Hoechst 33342; The levels of malondialdehyde (MDA), aspartate aminotransferase (AST), reduced glutathione (GSH), and superoxide dismutase (SOD) were examined; The expressions of iNOS and nitrotyrosine tubulin (NT) in APAP-induced LO2 hepatocyte injury cells was studied by Western blotting method. Results: TFFL had no toxicity to LO2 cells at a given concentration which can promote the proliferation. At the same time, experiment results showed that TFFL prophylactically reduced the apoptosis of LO2 cells induced by APAP. TFFL decreased the level of AST and MDA, increase the level of GSH and SOD, and inhibit the expressions of iNOS protein and NT protein. Conclusion: TFFL has a protective effect on LO2 cells injury induced by APAP. The possible protective mechanism of TFFL is related to the inhibition of oxidative stress and the nitro stress.

9.
Article in Chinese | WPRIM | ID: wpr-821726

ABSTRACT

Objective@#To evaluate the performance of a chromogenic agar developed by our laboratory for the isolation and culture of Clostridium difficile (CDCA). @*Methods@#The chromogenic specificity of CDCA was evaluated by inoculation of C. difficile and other standard strains, and the sensitivities of CDSA (BD), CDIF (BioMérieux) and CDCA were determined by the C. difficile standard strains respectively. A total of 120 clinical stool specimens were cultured for C. difficile by three chromogenic media respectively. The colonies were further identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and tpi gene was also detected. The sample which could be identified as C. difficile in any of the three chromogenic medium was defined as true positive. @*Results@#Most of standard strains were inhibited by CDCA, however some Clostridium species including C. clostridiiforme, C. bifermentans, C. tertium and Bacteroides fragilis grew lightly with chromogenic reaction. The sensitivities of CDSA, CDIF and CDCA were 2.0×105 CFU/mL, 8.0×101 CFU/mL and 4.0×10 2 CFU/mL, respectively. Among the 120 samples, 31 (25.8%) were defined as true C. difficile positive samples, while the positive rate of CDSA, CDIF and CDCA were 25 (20.8%), 28 (23.3%) and 26 (21.7%), respectively. There was no significant difference for clinical diarrhea specimens among the three chromogenic media (χ 2 =0.418, P=0.811). In comparison to the standard, the sensitivity, specificity, positive predictive value and negative predictive value were 83.8%, 100%, 100% and 94.7% for CDCA; 90.3%, 98.9%, 96.6% and 96.7% for CDIF; and 80.6%, 100%, 100% and 93.7% for CDSA. @*Conclusion@#The CDCA developed by our laboratory could be used to preliminarily isolate C. difficile with good specificity and sensitivity.

10.
Journal of Medical Postgraduates ; (12): 298-302, 2019.
Article in Chinese | WPRIM | ID: wpr-818231

ABSTRACT

Reduced nicotinamide adenine dinucleotide phosphate oxidase (NDAPH oxidase, NOX) is a complex composed of multiple protein subunits and the main enzyme produced by reactive oxygen species (ROS). It has been confirmed that NOXs-mediated oxidative stress is closely related to the occurrence and development of liver fibrosis, and its subunit NOX4 plays a key role. Nox4-derived ROS can not only directly affect the main cell of liver fibrosis ells (HSCs) with significant activation effects but also participate in the continuous activation of HSCs, abnormal proliferation of extracellular matrices(ECMs), apoptosis of hepatocytes, and migration of myofibroblasts(MFBs) by mediating a variety of intracellular signaling pathways including HSCs, which ultimately lead to the occurrence and development of liver fibrosis. This article reviews the role of NOX4-related signaling pathways in the pathogenesis of hepatic fibrosis.

11.
Article in Chinese | WPRIM | ID: wpr-753898

ABSTRACT

Objective To investigate the clinical characteristics of families with Huntington disease in Sichuan, and to make genetic diagnosis and pedigree analysis, and to provide genetic counseling for family members. Methods The detailed clinical data of Huntington disease patients and some family members who visited were collected from provincial people's Hospital of Sichuan between March 2016 and March 2018. The CAG trinucleotide repeats of IT15 gene were examined. The mini mental state examination (MMSE), Montreal cognitive scale (MoCA) were used to evaluate the cognitive function. Hamilton anxiety scale (HAMA), Hamilton Depression scale (HAMD) were used to evaluate the emotion. Activities of daily living scale (ADL) were used to evaluate the ability of daily life. Results Genetic test was conducted on twenty-four individuals from 4 families. Genetic test detected eight HD patients with 41-54 CAG repeats (46.75±4.03) and seven presymptomatic patients with 43~58 CAG repeats (50.00 ±6.40). Four of HD patients required genetic counseling for marriage and childbearing. The number of normal CAG repeats was 12~24, with 17 and 20 being the most common. Correlation analysis found that the number of CAG repeats was negatively correlated with the age of onset (r=-0.967, P<0.01). ADL score was positively correlated with course of disease (r=0.842, P<0.01), and negatively correlated with MMSE score (r=-0.930, P<0.01) and MoCA score (r=-0.932, P<0.01). Conclusion Genetic test is of great significance in the diagnosis of Huntington's disease, especially in patients with negative family history. The number of CAG repeats is increase from generation to generation and there is genetic anticipation in HD families. The number of CAG repeats can predict the onset age to some extent. Genetic counseling and prenatal diagnosis are important to avoid the birth of a child with HD.

12.
Article in Chinese | WPRIM | ID: wpr-753330

ABSTRACT

Objective To investigate the relationship between the stress induced glucose up-regulation and brainstem infarction. Methods Three hundred and sixty-six patients of cerebral infarction in the department of neurology were included in this study. Stress induced glucose up-regulation was reflected by stress induced glucose up-regulation ratio (SIGUT), and the level of SIGUT was compared between groups of brainstem and non-brainstem infarction. SIGUT was categorized into 4 groups according to the quartiles, and relative variables were compared among 4 groups. The level-risk relationship between high level of SIGUT and brainstem infarction was performed through logistic regression. Results The level of SIGUT was higher in group of brainstem infarction than that in group of non-brainstem infarction: 1.060 ± 0.272 vs.1.159 ± 0.301(P=0.020). The percent of male, diabetes, brainstem infarction and level of FBG, HbA1c, NIHSS was obviously different in the 4 groups of SIGUT according to the quartiles (P<0.05). High level of SIGUT was correlated to brainstem infarction in the single-factor or multivariable logistic regression adjusted for relative variable (P < 0.05). Conclusions Stress induced glucose up-regulation is correlated to the brainstem infarction, which is prone to happen in brainstem infarction, and the magnitude is more prominent.

13.
Article in Chinese | WPRIM | ID: wpr-773091

ABSTRACT

The aim of this paper was to investigate the preventive effects of Keluoxin Capsules(KLX) on diabetic retinopathy in db/db mice. One hundred male db/db diabetic mice(45-55 g, 8 weeks) were randomly divided into 5 groups(model, KLX low dose, KLX middle dose, KLX high dose, Dobesilate) and 20 male C57 BL/KsJdb~(+/+) were taken as control group. Body weight and fasting blood-glucose were detected every week. Mice were administrated with saline(control and model group), KLX(780, 1 560, 3 120 mg·kg~(-1)·d~(-1), ig), Dobesilate(195 mg·kg~(-1)·d~(-1), ig) for 20 weeks, respectively. At the end of the administration, optical coherence tomography, fundus fluorescein angiography and electroretinogram of the retina were measured. The eyeball was extirpated and retina was isolated to make paraffin section, followed by HE staining and glial fibrillary acidic protein(GFAP) immunohistochemistry. The results indicated that KLX has no obvious effect on body weight and fasting blood level in db/db mice. However, KLX could significantly regulate the thickness of retinal ganglion layer and inner plexiform layer. KLX was able to remarkably reduce the quantity of diabetic microvessel. Meanwhile, KLX could notably improve retinal function. Moreover, KLX could observably modulate the cell arrangement and edema in each layer. There was no markable difference in retina according to the immunochemistry assay. In the present study, KLX exert marked preventive effects on diabetic retinopathy in db/db mice, which provided an experimental evidence for clinical use.


Subject(s)
Animals , Capsules , Diabetes Mellitus, Experimental , Diabetic Retinopathy , Drug Therapy , Fluorescein Angiography , Hypoglycemic Agents , Pharmacology , Male , Mice , Random Allocation , Retina
14.
Article in Chinese | WPRIM | ID: wpr-772619

ABSTRACT

OBJECTIVE@#This study aimed to determine the clinical effectiveness of double-shaft classification and sequential diagnosis and treatment of food impaction.@*METHODS@#A total of 227 patients with food impactionwere subjected to double-shaft classification and subsequently treated by sequential diagnosis and treatment method. Treatment effectiveness was evaluated according to the subjective feelings of patientsat five time points (immediately after treatment and at 1, 3, 6, and 12 months after treatment).@*RESULTS@#The average scores of patient's subjective perception were 2.00, 1.93, 1.59, 1.20, and 0.98 immediately and at 1, 3, 6, and 12 months after treatment, with treatment-efficiency values of 87.17%, 81.70%, 73.21%, 55.80%, and 44.59%, respectively. There were differences in scores of patient's subjective perception at each time point (P<
0.05).@*CONCLUSIONS@#Double-shaft classification and sequential diagnosis and treatment can effectively guide the treatment of food impaction.


Subject(s)
Food , Humans , Treatment Outcome
15.
Article in Chinese | WPRIM | ID: wpr-744537

ABSTRACT

Objective To explore the expression of miRLet-7 family members in breast cancer and its correlation with overall survivals (OS), and to find more effective molecular targets for breast cancer prevention and treatment.Methods Kaplan-Meier (KM) plotter online database was used to analyze the correlation among the expression of Let-7 family members (Let-7a, 7b, 7c, 7d, 7e, 7f, 7g, 7i, miR-98, miR-202) correlated with overall survival (OS) and the prognosis and clinical pathological parameters of breast cancer patients, and Hazard ratio (HR), 95%confidence interval (CI), and P value were determined.ResultsThe study showed that the high expression level of Let-7a, Let-7b, Let-7c, Let-7e, Let-7f, Let-7g, miR-98 and the low expression level of miR-202 was associated with better OS for breast cancer patients (P<0.05).We further assessed the prognostic value of Let-7 in different subtypes and clinical stage.The expression of Let-7a, Let-7b, Let-7f, Let-7g, miR-98, miR-202 was related to clinical stage (P<0.05).Let-7a, Let-7b, Let-7c, Let-7e, Let-7f, Let-7g, miR-98 and miR-202 was related to lymph node status (P<0.05).In triple-negative breast cancers (TNBC), with breast cancer subtype, the expression of Let-7b, Let-7c, Let-7g and miR-202 was significantly correlated to overall survival (P<0.05).Conclusion The Let-7 is significantly correlated with OS in breast cancer patients.The results suggested that members of the Let-7 have different values in predicting the prognosis of breast cancer.Among them, Let-7b, Let-7g and miR-202 are closely related with clinical stage and TNBC, and might promote development of Let-7 as targeted inhibitors for the treatment of breast cancer.

16.
Article in Chinese | WPRIM | ID: wpr-851611

ABSTRACT

Objective To evaluate the anti-dysmenorrhea efficacy of habitat fresh-cut processed and traditional processed Linderae Radix based on the dysmenorrhea model. Methods By using the estrogen-induced dysmenorrhea model of ICR mice, the effect of treatment from two different processing methods on dysmenorrhea was compared by recording the writhing reaction as well as the changes of levels of prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α), vasopressin (AVP), and β-endorphin (β-EP). Results Compared with the model group, Linderae Radix from two different processing methods significantly reduced the frequency of writhing (P 0.05). Conclusion The two processing methods of Linderae Radix both have good therapeutic effect on dysmenorrhea model mice, and the effect of habitat processed Linderae Radix is not inferior to that of traditional processed Linderae Radix.

17.
Frontiers of Medicine ; (4): 196-205, 2018.
Article in English | WPRIM | ID: wpr-772750

ABSTRACT

We employed a multiplex polymerase chain reaction (PCR) coupled with capillary electrophoresis (mPCR-CE) targeting six Clostridium difficile genes, including tpi, tcdA, tcdB, cdtA, cdtB, and a deletion in tcdC for simultaneous detection and characterization of toxigenic C. difficile directly from fecal specimens. The mPCR-CE had a limit of detection of 10 colony-forming units per reaction with no cross-reactions with other related bacterial genes. Clinical validation was performed on 354 consecutively collected stool specimens from patients with suspected C. difficile infection and 45 isolates. The results were compared with a reference standard combined with BD MAX Cdiff, real-time cell analysis assay (RTCA), and mPCR-CE. The toxigenic C. difficile species were detected in 36 isolates and 45 stool specimens by the mPCR-CE, which provided a positive rate of 20.3% (81/399). The mPCR-CE had a specificity of 97.2% and a sensitivity of 96.0%, which was higher than RTCA (x = 5.67, P = 0.017) but lower than BD MAX Cdiff (P = 0.245). Among the 45 strains, 44 (97.8%) were determined as nonribotype 027 by the mPCR-CE, which was fully agreed with PCR ribotyping. Even though ribotypes 017 (n = 8, 17.8%), 001 (n = 6, 13.3%), and 012 (n = 7, 15.6%) were predominant in this region, ribotype 027 was an important genotype monitored routinely. The mPCR-CE provided an alternative diagnosis tool for the simultaneous detection of toxigenic C. difficile in stool and potentially differentiated between RT027 and non-RT027.


Subject(s)
Clostridium Infections , Diagnosis , Clostridioides difficile , Genetics , Electrophoresis, Capillary , Feces , Microbiology , Genes, Bacterial , Humans , Polymerase Chain Reaction , Ribotyping , Sensitivity and Specificity
18.
Article in Chinese | WPRIM | ID: wpr-775339

ABSTRACT

Scopolin (SC-1), scopoletin (SC-2) and isofraxidin (IS-1) are the main active constituents in Chimonanthi Radix. However, the in vivo metabolism of SC-1, SC-2 and IS-1 have not been comprehensively clarified. In this study, the in vivo metabolic profiles of these three coumarins in the rat plasma, urine and feces were analyzed. Ultra-high performance liquid chromatography-quadrupole time of flight mass spectrometry (UHPLC-QTOF-MS/MS) method was applied to characterize the prototypes and metabolites of SC-1, SC-2 and IS-1 in rat feces, urine, and plasma after intravenous administration. A total of 11 metabolites of the three parent compounds were tentatively identified. The main metabolic pathways were analyzed by identification of metabolites, and it was found that these three coumarins underwent multiple in vivo metabolic reactions including glucuronidation, sulfonation, isomerism and reduction. In this study, the analysis of metabolites of three coumarins basically demonstrated their in vivo metabolic process, providing basis for the further pharmacokinetics and pharmacological evaluations of SC-1, SC-2 and IS-1.


Subject(s)
Animals , Calycanthaceae , Chemistry , Chromatography, High Pressure Liquid , Coumarins , Metabolism , Pharmacokinetics , Drugs, Chinese Herbal , Metabolism , Pharmacokinetics , Rats , Tandem Mass Spectrometry
19.
Article in Chinese | WPRIM | ID: wpr-695875

ABSTRACT

Objective To evaluate the therapeutic efficacy of abdomen-relaxation-vibration therapy in treating refractory insomnia. Method Eighty patients with refractory insomnia were randomized into a treatment group (40 cases) and a control group (40 cases). The treatment group was intervened by abdomen-relaxation-vibration therapy, while the control group was intervened by oral administration of Diazepam prior to sleep. The clinical efficacy and change of Pittsburgh Sleep Quality Index (PSQI) in the two groups were observed. Result The scores of PSQI dropped significantly in both groups after the treatment (P<0.01), and the scores in the treatment group were significantly lower than those in the control group (P<0.01,P<0.05); the markedly effective rate and total effective rate were respectively 62.5% and 87.5% in the treatment group, significantly higher than 27.5% and 62.5% in the control group, and there was a significant difference in the therapeutic efficacy between the two groups (P<0.05). Conclusion Abdomen-relaxation-vibration therapy is effective in treating refractory insomnia, and it's easy-to-operate and safe.

20.
Article in Chinese | WPRIM | ID: wpr-695731

ABSTRACT

Objective·To analyze the basic medical insurance policies for children in Shanghai in 2011 and 2017, and compare with those of the other three municipalities to understand the basic medical security status of children in four municipalities in China and analyze the existing problems. Methods?·?The current effective children's basic medical insurance policies and child critical illness insurance policies and related insurance systems in four municipalities were collected systematically. The types of basic medical insurance for children in various municipalities and their funding standards, their own expenses and the proportion of government subsidies, the proportion of outpatient emergency payoff lines and reimbursement at all levels of medical institutions, and the coverage and coverage of major illness insurance reimbursement were collected and compared mainly. Results?·?The basic medical insurance for children in Shanghai is a "double insurance" system, which contained children's hospitalization fund and basic medical insurance for urban and rural residents. The basic medical insurance for children in other municipalities mainly consisted of basic medical insurance for urban (rural) residents. Compared with 2011, the level of financing and the reimbursement of the basic medical insurance for urban and rural residents and of the children's hospitalization fund in Shanghai in 2017 was increased. In 2017, the level of financing of basic medical insurance for urban and rural residents in Shanghai was 1?100 yuan, which was slightly lower than that of Beijing medical insurance for urban residents and new rural cooperative medical insurance and was higher than that of basic medical insurance for urban and rural residents in Tianjin and Chongqing, and the individual payment level of Shanghai was the lowest. The hospitalization payoff lines in Shanghai were lower than those of other municipalities. Shanghai's outpatient and inpatient reimbursement levels were higher those of than other municipalities. There wasn't a capping line in Shanghai. There were capping lines in other municipalities. There was a great difference in the insurance policy of the major diseases in four municipalities. Conclusion?·?According to their own actual conditions, models for children's medical care systems that suited each municipalities’ social and economic development have been established. Shanghai children's basic medical insurance basically meets the needs of children's growth but needs improvement in terms of the type and level of medical security for major illnesses.

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