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ABSTRACT OBJECTIVE:To study pharmacokinetic characteristics of single dose and multiple dose administration of Gefitinib emulsion in rats. METHODS:The rats were divided into single administration group and multiple administration group. Single administration group was subdivided into Gefitinib raw medicine group(50 mg/kg,i.g.)and Gefitinib emulsion group(50 mg/kg,i.g.),with 6 rats in each group,gavage once. Multiple administration group were subdivided into Gefitinib raw medicine group (50 mg/kg)and Gefitinib emulsion group(50 mg/kg),with 8 rats in each group;they were given relevant medicine intragastricaly for consecutive 7d,once a day. 0.3 mL blood of rats in Gefitinib raw medicine group was taken before medication and 1,2,2.5, 3,3.5,3.75,4,4.25,4.5,6,8,12 and 24 h after medication;0.3 mL blood of rats in Gefitinib emulsion group was taken before medication and 2,4,6,8,9,10,11,12,13,14,16,24,36 and 48 h after administration(Multiple administration group is after 7 d of administration). HPLC method was used to determine the plasma concentration of gefitinib in rat,and plasma concentration-time curves were drawn. Pharmacokinetic parameters were fitted by using DAS 2.0 software. RESULTS:After single administration,compared with the tmax([ 2.67±0.75)h],MRT0-24 h ([ 8.68±0.91)h],MRT0- ∞ ([ 14.20±3.45)h] of Gefitinib raw medicine group,tmax ([ 8.33±4.41)h],MRT0-48 h ([ 15.00±1.60)h],MRT0-∞ ([ 17.60±2.66)h] of Gefitinib emulsion group were increased significantly(P<0.05). After multiple administration,compared with the tmax ([ 6.79±3.75)h],AUC0-48 h ([ 41.10±8.92) mg·h/L],Vz/F [(16.30±5.45)L/kg],CLz/F [(0.94±0.19) L/(h·kg)],MRT0-48 h ([ 10.10 ± 0.36) h] of Gefitinib raw medicine group,Vz/F [(44.20±30.3)L/kg],CLz/F[(1.89± 1.56) L/(h·kg)],MRT0-48 h ([ 16.20 ± 2.52) h] of Gefitinib emulsion group were increased significantly (P<0.05) AUC0-48 h ([ 38.70±26.20)mg·h/L] was decreased significantly (P<0.05),and tmax ([ 10.40±3.25)h] was increased,without statistical significance. CONCLUSIONS: Compared with Gefitinib raw medicine,single and multiple administration of Gefitinib emulsion can effectively prolong the peak time,the results of this study can provide reference for new delivery system study of Gefitinib.
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A new method of dissolution test was established to better simulate the in vivo dissolution behavior of drugs from preparations and to distinguish the quality difference between drug preparations. With flow-through cell being chosen to be the dissolution apparatus and nimodipine tablet to be the model drugs,this study developed,on the basis of IVIVC theory,a new dissolution method which was subsequently used to evaluate the dissolution con-sistency of domestically produced nimodipine tablet as test preparation and its reference preparation. Meanwhile, conventional four-dissolution-curves method based on paddle apparatus was selected for comparison to evaluate the efficiency of the new dissolution method. The results indicated that the new dissolution method not only had a good correlation with the in vivo process of drugs,but also could reveal the internal quality differences between pharmaceutical preparations effectively. This research will provide further theoretical support for the application of flow-through cell apparatus in IVIVC study.
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Aim To investigate the effect of SM-1 on seven main cytochrome P450(CYP450)in human liver microsomes.Methods Substrate or SM-1 was incubated with human liver microsomes for 30 min in vitro,and divided into control group and experimental group.The effects of SM-1 on the main phase I metabolic enzymes in human liver microsomes was detected by HPLC.Phenacetin,bupropion,paclitaxel,tolbutamide,omeprazole,dextromethorphan,testosterone were investigated as probe drugs.Results Inhibition rate of SM-1 on the classical substrate of human liver microsomal CYP was 0.05%,3.37%,0.08%,2.07%,4.20%,-0.15%and 10.84%,respectively.Conclusions SM-1 may have inhibitory effect on CYP3A4.Attention should be paid to the interaction of clinical drug induced by CYP enzyme inhibition.
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The aim of this study was to apply the reference-scaled average bioequivalence (RSABE) approach to evaluate the bioequivalence of 2 formulations of agomelatine, and to investigate the pharmacokinetic properties of agomelatine in Chinese healthy male subjects. This was performed in a single-dose, randomized-sequence, open-label, four-way crossover study with a one-day washout period between doses. Healthy Chinese males were randomly assigned to receive 25 mg of either the test or reference formulation. The formulations were considered bioequivalent if 90% confidence intervals (CIs) for the log-transformed ratios and ratio of geometric means (GMR) of AUC and C max of agomelatine were within the predetermined bioequivalence range based on RSABE method. Results showed that both of the 90% CIs for the log-transformed ratios of AUC and C max of 7-desmethyl-agomelatine and 3-hydroxy-agomelatine were within the predetermined bioequivalence range. The 90% CIs for natural log-transformed ratios of C max, AUC0-t and AUC0-∞ of agomelatine (104.42-139.86, 101.33-123.83 and 97.90-117.94) were within the RSABE acceptance limits, and 3-hydroxy-agomelatine (105.55-123.03, 101.95-109.10 and 101.72-108.70) and 7-desmethyl-agomelatine (104.50-125.23, 102.36-111.50 and 101.62-110.64) were within the FDA bioequivalence definition intervals (0.80-1.25 for AUC and 0.75-1.33 for C max). The RSABE approach was successful in evaluating the bioequivalence of these two formulations.
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Objective To determine the pharmacokinetic interaction between cefalor and bromhexine in healthy Chinese volunteers. Methods Twelve subjects received a cefaclor (CEF) treatment, a bromhexine (BHX) treatment, and a co-treatment of CEF and BHX with a 3 × 3 Latin square design. The wash-out time between periods was 14 days. The plasma and urine drug concentrations of CEF and BHX were detected by HPLC-UV and LC/MS, respectively. Results All the 12 volunteers completed the study. There were no significant differences in AUC0-t and Cmax of CEF in logarithm between the single administration group of CEF and the co-administration group of CEF with BHX. Two one sided t-test showed that CEF was bioequivalent in the 2 groups. There were no significant differences in tmax, MRT, t1/2, and Clr between the 2 groups. Vd/F was significantly lower in the single CEF group than in the co-administration group of CEF and BHX. There were no significant differences of AUC0-t and Cmax of BHX in logarithm between the single administration group of BHX and the co-administration group of BHX with CEF. Two one sided t-test showed that BHX was bioequivalent in the 2 groups. There were no significant differences in tmax, MRT, t1/2, Vd/F, and Clr between the 2 groups. Conclusion There is no significant pharmacokinetic parameter change in the drug absorption, metabolism, and excretion, but Va/F of CEF significant increases in the co-administration of CEF with BHX. The co-administration of CEF and BHX has no adverse drug interaction. The increase of Vd/F may be a favorable drug interaction, which may be the mechanism of the synergistic effect of the 2 drugs.
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0.05)in the main pharmacokinetic parameters between the domestic preparation and the imported preparation,which suggests they are bioequivalent.
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OBJECTIVE:To study the pharmacokinetics of prulifloxacin capsules in Chinese healthy volunteers after single and multiple oral administration of prulifloxacin capsules.METHODS:A total of 12 healthy adult subjects were randomly grouped by 3? 3 Latin square,who were assigned to receive oral single dose of 132,264 and 528mg prulifloxacin capsules and multiple doses of 264mg prulifloxacin capsule for 6 days in succession.The blood concentration of NM394-the metabolite of Prulifloxacin was determined by HPLC at different time after oral administration of Prulifloxacin.The simulation and fitting,and computation of parameters were performed using DAS ver1.0 software.RESULTS:All 12 subjects had completed single oral administration test,with no adverse drug reactions appeared during the test.No prulifloxacin but its metabolite-NM394 was identified in the blood sample of subjects.The high,medium and low dosage groups were all fitted two-compartment model.The pharmacokinetics fitted first order kinetics process without gender difference.There was no accumulation and pharmacokinetic parameters change after multiple oral administration of prulifloxacin,suggesting prulifloxacin had no self-enzyme inhibition or induction.CONCLUSION:The established method is sensitive,accurate,reliable and specific,and it can meet the requirement of clinical pharmacokinetic trial.Its parameters are in line with literature reported abroad,with no gender difference among Chinese adults.
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OBJECTIVE: A gargle was designed and prepared for treating immune inpairment and anaerobic infection of oral cavity .The method of its quantitative analysis was established and put into clinical use. METHODS: HPLC was used as the quantitative analysis method .The effect on oral lichen planus was clinically observed. RESULTS: The taste of the gargle was well acceptable, and its quality was well controlled.The cure rate for oral lichen planus was 96%. No adverse reactions were found. CONCLUSION: The taste and method of quantitative analysis are well acceptable. The clinical effect is satisfactory.