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1.
Asian Journal of Andrology ; (6): 213-218, 2022.
Article in English | WPRIM | ID: wpr-928528

ABSTRACT

Experimental autoimmune prostatitis (EAP)-induced persistent inflammatory immune response can significantly upregulate the expression of N-methyl-D-aspartic acid (NMDA) receptors in the paraventricular nucleus (PVN). However, the mechanism has not yet been elucidated. Herein, we screened out the target prostate-derived inflammation cytokines (PDICs) by comparing the inflammatory cytokine levels in peripheral blood and cerebrospinal fluid (CSF) between EAP rats and their controls. After identifying the target PDIC, qualified males in initial copulatory behavior testing (CBT) were subjected to implanting tubes onto bilateral PVN. Next, they were randomly divided into four subgroups (EAP-1, EAP-2, Control-1, and Control-2). After 1-week recovery, EAP-1 rats were microinjected with the target PDIC inhibitor, Control-1 rats were microinjected with the target PDIC, while the EAP-2 and Control-2 subgroups were only treated with the same amount of artificial CSF (aCSF). Results showed that only interleukin-1β(IL-1β) had significantly increased mRNA-expression in the prostate of EAP rats compared to the controls (P < 0.001) and significantly higher protein concentrations in both the serum (P = 0.001) and CSF (P < 0.001) of the EAP groups compared to the Control groups. Therefore, IL-1β was identified as the target PDIC which crosses the blood-brain barrier, thereby influencing the central nervous system. Moreover, the EAP-1 subgroup displayed a gradually prolonged ejaculation latency (EL) in the last three CBTs (all P < 0.01) and a significantly lower expression of NMDA NR1 subunit in the PVN (P = 0.043) compared to the respective control groups after a 10-day central administration of IL-1β inhibitors. However, the Control-1 subgroup showed a gradually shortened EL (P < 0.01) and a significantly higher NR1 expression (P = 0.004) after homochronous IL-1β administration. Therefore, we identified IL-1β as the primary PDIC which shortens EL in EAP rats. However, further studies should be conducted to elucidate the specific molecular mechanisms through which IL-1β upregulates NMDA expression.


Subject(s)
Animals , Cytokines/metabolism , Disease Models, Animal , Ejaculation/physiology , Interleukin-1beta/metabolism , Male , N-Methylaspartate/metabolism , Prostate/metabolism , Prostatitis/metabolism , Rats , Receptors, N-Methyl-D-Aspartate/metabolism
2.
National Journal of Andrology ; (12): 122-127, 2018.
Article in Chinese | WPRIM | ID: wpr-775209

ABSTRACT

Objective@#To investigate the success rate and safety of percutaneous vasoseminal vesiculography with the disposable vasographic interventional therapy kit (VITK).@*METHODS@#This study included ninety-six 19-65 (mean 43) years old male patients with infertility, hematospermia, seminal vesicle cyst, ejaculatory duct cyst, ejaculatory dysfunction, or vas deferens injury, with disease courses varying from 1 month to 7 years. With an open, multi-centered, single-group, self-controlled design and using the disposable VITK, we treated the patients by percutaneous vasoseminal vesiculography via injection of contrast medium into the vas deferens cavity under local anesthesia.@*RESULTS@#Percutaneous vasoseminal vesiculography was successfully performed in 92 (97.87%) of the patients, which revealed abnormal seminal ducts in 51 cases (54.3%). Among the 28 infertile patients, 3 were found with bilateral and 5 with unilateral vas deferens obstruction. Vesiculitis was detected in 36 (81.8%) of the 44 hematospermia patients and bilateral vas deferens abnormality in 5 (38.5%) of the 13 patients with ejaculatory dysfunction. Transectional damage was observed in 2 patients with vas deferens injury induced by bilateral inguinal hernia repair. Three cases of seminal vesicle cyst and 4 cases of ejaculatory cyst were definitely diagnosed by vasoseminal vesiculography.@*CONCLUSIONS@#The disposable vasographic interventional therapy kit, with the advantages of simple operation and high safety, deserves a wide clinical application in vasoseminal vesiculography.


Subject(s)
Adult , Aged , Contrast Media , Cysts , Diagnostic Imaging , Ejaculatory Ducts , Diagnostic Imaging , Genital Diseases, Male , Diagnostic Imaging , Hemospermia , Diagnostic Imaging , Hernia, Inguinal , General Surgery , Humans , Infertility, Male , Diagnostic Imaging , Injections , Male , Middle Aged , Postoperative Complications , Diagnostic Imaging , Radiography , Methods , Seminal Vesicles , Diagnostic Imaging , Vas Deferens , Diagnostic Imaging , Wounds and Injuries , Young Adult
3.
National Journal of Andrology ; (12): 302-308, 2017.
Article in Chinese | WPRIM | ID: wpr-812769

ABSTRACT

Objective@#To explore the effect of the AXL expression on the chemosensitivity of prostate cancer PC-3 and DU145 cells to docetaxel and possible mechanisms.@*METHODS@#Using Western blot, we examined the expressions of the AXL protein, p-AXL and Gas6 in the docetaxel-resistant PC-3 (PC-3-DR) and DU145 (DU145-DR) cells stimulated with gradually increased concentrations of docetaxel. We transfected the PC-3 and DU145 cells with negative NC ShRNA and AXL-ShRNA, respectively, which were confirmed to be effective, detected the proliferation, apoptosis and cycle distribution of the cells by CCK8, MTT and flow cytometry after treated with the AXL-inhibitor MP470 and/or docetaxel, and determined the expression of the ABCB1 protein in the PC-3-DR and DU145-DR cells after intervention with the AXL-inhibitor R428 and/or docetaxel.@*RESULTS@#The expression of the AXL protein in the PC-3 and DU145 cells was significantly increased after docetaxel treatment (P <0.05). The expressions AXL and p-AXL were remarkably higher (P <0.05) while that of Gas6 markedly lower (P <0.05) in the PC-3 and DU145 than in the PC-3-DR and DU145-DR cells. The inhibitory effect of docetaxel on the proliferation and its enhancing effect on the apoptosis of the PC-3 and DU145 cells were significantly decreased at 48 hours after AXL transfection (P <0.05). MP470 obviously suppressed the growth and promoted the apoptosis of the PC-3-DR and DU145-DR cells, with a higher percentage of the cells in the G2/M phase when combined with docetaxel than used alone (P <0.05). R428 markedly reduced the expression of ABCB1 in the PC-3-DR and DU145-DR cells, even more significantly in combination with docetaxel than used alone (P <0.05).@*CONCLUSIONS@#The elevated expression of AXL enhances the docetaxel-resistance of PC-3 and DU145 prostate cancer cells and AXL intervention improves their chemosensitivity to docetaxel, which may be associated with the increased cell apoptosis in the G2/M phase and decreased expression of ABCB1.


Subject(s)
ATP Binding Cassette Transporter, Subfamily B, Member 1 , Metabolism , Antineoplastic Agents , Pharmacology , Apoptosis , Cell Count , Cell Cycle , Cell Line, Tumor , Cell Proliferation , Docetaxel , Drug Resistance, Neoplasm , Humans , Intercellular Signaling Peptides and Proteins , Metabolism , Male , Prostatic Neoplasms , Drug Therapy , Metabolism , Pathology , Proto-Oncogene Proteins , Genetics , Metabolism , Pyrimidines , Pharmacology , RNA, Small Interfering , Receptor Protein-Tyrosine Kinases , Genetics , Metabolism , Taxoids , Pharmacology
4.
National Journal of Andrology ; (12): 617-620, 2016.
Article in Chinese | WPRIM | ID: wpr-262344

ABSTRACT

<p><b>Objective</b>To assess the effect of corporoplasty with autologous tunica vaginalis graft in the treatment of Peyronie's disease.</p><p><b>METHODS</b>Ten patients with Peyronie's disease underwent plaque excision and corporoplasty with autologous tunica vaginalis graft. We obtained and compared IIEF-5 scores of the patients before and at 1 and 5 years after operation.</p><p><b>RESULTS</b>After surgery, penile curvature was obviously relieved and all the patients achieved normal penile erection and satisfactory sexual intercourse without erection-related pain or recurrent erectile dysfunction. The mean IIEF-5 score was significantly improved at 1 year (22.40±1.08) and 5 years postoperatively (23.00±1.14) as compared with the baseline, (19.20±2.28) (P<0.05 or 0.01).</p><p><b>CONCLUSIONS</b>Corporoplasty with autologous tunica vaginalis graft is a safe, simple and effective option for the treatment of Peyronie's disease, though its definite efficiency is to be further supported by large-sample clinical studies.</p>


Subject(s)
Erectile Dysfunction , Humans , Male , Penile Erection , Penile Induration , General Surgery , Penis , General Surgery , Postoperative Period , Testis , Transplantation
5.
National Journal of Andrology ; (12): 598-603, 2015.
Article in Chinese | WPRIM | ID: wpr-276052

ABSTRACT

<p><b>OBJECTIVE</b>To translate the English version of The Premature Ejaculation Diagnostic Tool (PEDT) into Chinese, evaluate its reliability and validity, and analyze its feasibility in the diagnosis of premature ejaculation (PE).</p><p><b>METHODS</b>Following the forward-backward translation procedure, we developed the Chinese version of PEDT, which was then revised by andrologists and bilingual linguists. We enrolled subjects with or without PE from 15 urological or andrological clinics in China and obtained the information about their demographic characteristics, PEDT scores, and intra-vaginal ejaculation latency time (IELT). We evaluated the internal consistency of PEDT using Cronbach alpha, was examined its reliability and stability by test-retest analysis, analyzed its correlation with IELT by Spearman correlation analysis, and tested its sensitivity and specificity by receiver operating characteristic ( ROC) analysis.</p><p><b>RESULTS</b>Totally, 570 PE patients (aged [30.66 ± 7.11] years) and 226 non-PE men (aged [33.01 ± 5.41] years) were recruited, with the mean IELT of (1.34 ± 0.54) min in the former and (11.09 ± 7.5) min in the latter group. The Cronbach's alpha of the Chinese version of PEDT was 0.79, and the test-retest correlation coefficient was 0.75 (P < 0.01). The PEDT score was negatively correlated with IELT (Spearman's p = -0.52, P < 0.01). When the cutoff value of PE diagnosis was defined as 7.5, the sensitivity and specificity of PEDT were 0.80 and 0.78, and when as 8.5, they were 0.72 and 0.89, respectively.</p><p><b>CONCLUSION</b>The Chinese version of PEDT was demonstrated to have good internal consistency, reliability, and validity, as well as a high predictability for PE. It can be used as a reliable and convenient tool to screen PE among Chinese men.</p>


Subject(s)
Adult , Aged , Asians , China , Ejaculation , Feasibility Studies , Humans , Language , Male , Middle Aged , Premature Ejaculation , Diagnosis , ROC Curve , Reaction Time , Reproducibility of Results , Sensitivity and Specificity , Translations
6.
National Journal of Andrology ; (12): 1125-1128, 2015.
Article in Chinese | WPRIM | ID: wpr-304762

ABSTRACT

Prolactin is a polypeptide hormone which mainly acts on the reproductive system and plays an important role in penile erection and ejaculation. Prolactin receptors have a variety of short forms apart from the classic long form, which are widely expressed in male reproductive glands. High levels of prolactin can induce erectile dysfunction and results in secondary male infertility, which are mainly associated with the inhibition of dopaminergic activity, reduction of the testosterone level, and contraction of the cavernous smooth muscle. Moreover, low levels of prolactin can result in ejaculatory dysfunction. This article updates the views on the expressions of prolactin receptors in the male reproductive system, the effects of prolactin on penile erection and ejaculation, and its action mechanisms.


Subject(s)
Ejaculation , Physiology , Erectile Dysfunction , Humans , Infertility, Male , Male , Muscle, Smooth , Penile Erection , Physiology , Prolactin , Physiology , Receptors, Prolactin , Physiology , Reproduction
7.
National Journal of Andrology ; (12): 647-650, 2014.
Article in Chinese | WPRIM | ID: wpr-309660

ABSTRACT

DAZL, a member of the DAZ family, plays a key role in human spermatogenesis. It regulates the promoter via genetic modification, especially DNA methylation, promoting the transcription of DAZL. Besides, the relation of DAZL SNPs with male infertility has been a hot spot of research for many years. It is deduced that local and ethnic factors may be associated with the failure of spermatogenesis. This article presents an overview on the results of recent studies, changes in the DNA methylation of DAZL in spermatogenesis, and the association of DAZL SNPs with male infertility, aiming to provide a new theoretical basis and clinical strategy for the treatment of male infertility.


Subject(s)
DNA Methylation , Humans , Infertility, Male , Genetics , Male , Polymorphism, Single Nucleotide , RNA-Binding Proteins , Genetics , Spermatogenesis
8.
National Journal of Andrology ; (12): 1020-1024, 2014.
Article in Chinese | WPRIM | ID: wpr-319575

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the safety and feasibility of testis-sparing surgery (TSS) in the treatment of testicular tumor.</p><p><b>METHODS</b>We retrospectively analyzed the clinical data of 8 cases of benign testicular tumor treated by TSS in our hospital from October 2005 to March 2012.</p><p><b>RESULTS</b>The 8 patients, aged 18-67 (mean 45) years, were preoperatively diagnosed with benign testicular tumor and all underwent partial testis resection. Rapid intraoperative pathology showed the incisal margins to be negative. Postoperative pathological examination confirmed Sertoli cell tumor in 3 cases, adenomatoid tumor in another 3, and mature teratoma in the other 2. The patients were followed up for 6 months to 7 years (mean 4 years), which revealed no relapse and metastasis, nor significant differences from the baseline in the testosterone level, IIEF score, and routine semen parameters.</p><p><b>CONCLUSION</b>Testis-sparing surgery is one of the effective options for the management of benign testicular tumor, which can maximally preserve the testis tissue and protect the patient's sexual function.</p>


Subject(s)
Adolescent , Adult , Aged , Humans , Male , Middle Aged , Neoplasm Recurrence, Local , Organ Sparing Treatments , Methods , Retrospective Studies , Sertoli Cell Tumor , Pathology , General Surgery , Teratoma , Pathology , General Surgery , Testicular Neoplasms , Pathology , General Surgery , Testis
9.
National Journal of Andrology ; (12): 798-801, 2013.
Article in Chinese | WPRIM | ID: wpr-267999

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the safety of assisted reproductive technology (ART) with donated sperm from the sperm bank and the differences in the pregnancy outcomes of different means of promoting pregnancy.</p><p><b>METHODS</b>We analyzed and compared the feedback data on promoting pregnancy with donated sperm from the sperm bank by artificial insemination by donor (AID), in vitro fertilization (IVF), and intracytoplasm sperm injection (ICSI).</p><p><b>RESULTS</b>Totally, 13 723 tubes of sperm specimens were used for ART. The number of specimens used differed in different clinical reproductive centers, some using 1 tube and others using 2 tubes per cycle. The 13 723 tubes were used for a total of 7 743 cycles. Among the 7 123 cycles of AID, there were 1 415 clinical pregnancies (19.87%), 1 221 normal births (86.29%), 169 abortions (11.94%), 6 cases of birth defects (0.43%), 19 ectopic pregnancies (1.34%), and 0 sexually transmitted infection. Among the 571 cycles of IVF, there were 367 clinical pregnancies (64.27%), 330 normal births (89.92%), 35 abortions (9.54%), 0 birth defect, 2 ectopic pregnancies (0.54%), and 0 sexually transmitted infection. Among the 49 cycles of ICSI, there were 28 clinical pregnancies (57.14%), 25 normal births (89.29%), 3 abortions (10.71%), 0 birth defect, 0 ectopic pregnancy, and 0 sexually transmitted infection. There were statistically significant differences in the rate of clinical pregnancy among AID, IVF and ICSI (P < 0.05), but not between IVF and ICSI (P > 0.05), nor were there any significant differences in the rates of abortion, birth defects and ectopic pregnancy among AID, IVF and ICSI (P > 0.05).</p><p><b>CONCLUSION</b>None of the recipients of the donated sperm from the sperm bank was infected with sexually transmitted diseases. AID, IVF and ICSI showed no significant differences from natural conception in the rates of abortion, birth defects and ectopic pregnancy. ART with donated sperm from the sperm bank is safe. IVF and ICSI are associated with a higher rate of pregnancy than AID, though the latter costs less than the former two.</p>


Subject(s)
Female , Fertilization in Vitro , Humans , Male , Pregnancy , Pregnancy Outcome , Pregnancy Rate , Sperm Banks , Sperm Injections, Intracytoplasmic , Spermatozoa
10.
National Journal of Andrology ; (12): 1091-1094, 2013.
Article in Chinese | WPRIM | ID: wpr-267982

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the clinical effect and application value of the modified method of prepuce-degloving repair (PDR) in the treatment of urethrocutaneous fistula (UCF) following hypospadias surgery.</p><p><b>METHODS</b>We retrospectively analyzed 87 cases of UCF caused by hypospadias repair from May 2001 to December 2011, of which 61 were treated by simple closure or Y-V plasty of the fistula (group A), and the other 26 by modified PDR (group B). We compared the success rate of surgery and long-term recurrence between the two groups.</p><p><b>RESULTS</b>The total success rates of repair were 78.7 and 96.2% in groups A and B, respectively. Thirteen cases in group A did not respond to surgery, of which 6 failed to be cured by the second operation but later were treated successfully by modified PDR. In comparison, there was only 1 case of failure in group B, which was cured by a second PDR.</p><p><b>CONCLUSION</b>Modified PDR can significantly improve the success rate and reduce the recurrence rate of UCF after hypospadias surgery, which deserves wide clinical application.</p>


Subject(s)
Child , Child, Preschool , Foreskin , General Surgery , Humans , Hypospadias , General Surgery , Male , Reconstructive Surgical Procedures , Methods , Retrospective Studies , Treatment Outcome , Urinary Fistula , General Surgery , Urologic Surgical Procedures, Male
11.
National Journal of Andrology ; (12): 619-622, 2012.
Article in Chinese | WPRIM | ID: wpr-286435

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the safety and feasibility of glans-preserving surgery in the treatment of superficial penile cancer (SPCa).</p><p><b>METHODS</b>We retrospectively analyzed the clinical data of 21 cases of SPCa treated by glans-preserving surgery in our hospital from January of 2003 to March of 2010.</p><p><b>RESULTS</b>The study included 21 SPCa patients aged 36 to 57 (mean 46) years, with superficial lesions involving the glans penis, coronary sulcus or shaft skin. The tumors were staged and graded TaG1 in 6 cases, TaG2 in 5, TisG1 in 2, TisG2 in 4, T1G1 in 3, and T1G2 in 1. All the patients underwent glans-preserving surgery to preserve the normal appearance and functional integrity of the glans penis, and all returned to normal sexual activity 1 month after operation, with good sexual function and sexual satisfaction. Postoperative follow-up lasted 2 to 7 (mean 5) years, and 2 cases of recurrence in situ found at 6 and 9 months, respectively, which were successfully managed by a second glans-preserving surgery.</p><p><b>CONCLUSION</b>Glans-preserving surgery is an effective method for superficial penile cancer. With proper selection of the patients, this procedure is technically safe, maximally preserves the penile appearance, and least affects the patients' sexual satisfaction.</p>


Subject(s)
Adult , Carcinoma, Squamous Cell , General Surgery , Humans , Male , Middle Aged , Penile Neoplasms , General Surgery , Penis , General Surgery , Retrospective Studies , Treatment Outcome
12.
Chinese Journal of Urology ; (12): 373-375, 2010.
Article in Chinese | WPRIM | ID: wpr-671334

ABSTRACT

Objective To discuss the treatment of ureteropelvic junction obstruction by laparoscopic pyeloplasty. Methods A retrospective review of consecutive laparoscopic pyeloplasty in 102 patients between September 2001 and December 2007 was performed. The ureterpelvic junction was dissected and the obstruction portion was excised. Anastomosis was then performed through the ureter and the renal pelvis walls with a stent. Results The mean operating time was 120 min and the average blood loss was 80ml. No major complication occurred intraoperative. The drainage was removed in 3-10 days. The average hospital stay was 8.5 days. The stent was kept for 30-60 days. IVU and B ultrasound examination revealed that the hydronephrosis alleviated during the follow-up and no anastomosis stricture occurred. Conclusions Laparoscopic dismembered pyeloplasty could provide lower morbidity, shorter hospital stay, and faster convalescence. It could be an effective treatment for ureteropelvic junction obstruction.

13.
National Journal of Andrology ; (12): 646-650, 2009.
Article in Chinese | WPRIM | ID: wpr-241282

ABSTRACT

There are over 200 secretive proteins in the epididymis. Spermatozoa are generally considered to become mature and full-functional after interacting with secretive proteins in the epididymis. This review is aimed at summarizing some aspects of the biochemical, molecular and functional characterization of some new proteins recently detected in human epididymis, and is expected to contribute to further researches on the mechanism of epididymal reproduction and contraception.


Subject(s)
Epididymis , Bodily Secretions , Humans , Male , Proteins , Metabolism , Spermatozoa , Metabolism , Sugar Alcohol Dehydrogenases , Metabolism
14.
National Journal of Andrology ; (12): 364-366, 2009.
Article in Chinese | WPRIM | ID: wpr-292368

ABSTRACT

Sperm acquires capacity of motility and fertility during the process of semen coagulation and liquefaction. The main coagulative protein is Semenogelin I (Sg I), specifically produced by seminal vesicles, and then decomposed by prostate specific antigens (PSA) in sperm liquefaction into a series of small fragments. These fragments, with a variety of physiological functions, are very important for the regulation of sperm capacity acquisition and progressive movement.


Subject(s)
Humans , Male , Seminal Vesicle Secretory Proteins , Physiology , Seminal Vesicles , Metabolism , Physiology
15.
Asian Journal of Andrology ; (6): 770-775, 2008.
Article in English | WPRIM | ID: wpr-359911

ABSTRACT

<p><b>AIM</b>To study the molecular mechanism of epididymal protease inhibitor (Eppin) modulating the process of prostate specific antigen (PSA) digesting semenogelin (Sg).</p><p><b>METHODS</b>Human Sg cDNA (nucleotides 82-849) and Eppin cDNA (nucleotides 70-723) were generated by polymerase chain reaction (PCR) and cloned into pET-100D/TOPO. Recombinant Eppin and Sg (rEppin and rSg) were produced by BL21 (DE3). The association of Eppin with Sg was studied by far-western immunoblot and radioautography. In vitro the digestion of rSg by PSA in the presence or absence of rEppin was studied. The effect of anti-Q20E (N-terminal) and C-terminal of Eppin on Eppin-Sg binding was monitored.</p><p><b>RESULTS</b>Eppin binds Sg on the surface of human spermatozoa with the C-terminal of Eppin (amino acids 75-133). rSg was digested with PSA and many low molecular weight fragments were produced. When rEppin is bound to rSg, then digested by PSA, incomplete digestion and a 15-kDa fragment results. Antibody binding to the N-terminal of rEppin did not affect rSg digestion. Addition of antibodies to the C-terminal of rEppin inhibited the modulating effect of rEppin.</p><p><b>CONCLUSION</b>Eppin protects a 15-kDa fragment of rSg from hydrolysis by PSA.</p>


Subject(s)
Animals , Antibodies , Pharmacology , Autoradiography , Humans , Hydrolysis , Male , Prostate-Specific Antigen , Metabolism , Proteinase Inhibitory Proteins, Secretory , Genetics , Allergy and Immunology , Metabolism , Rabbits , Recombinant Proteins , Genetics , Metabolism , Semen , Cell Biology , Metabolism , Seminal Vesicle Secretory Proteins , Metabolism , Spermatozoa , Metabolism
16.
National Journal of Andrology ; (12): 42-45, 2007.
Article in Chinese | WPRIM | ID: wpr-289049

ABSTRACT

<p><b>OBJECTIVE</b>To study the inhibition activity of Semenogelin (Sg) and its different peptides to human spermatozoa.</p><p><b>METHODS</b>Human Sg DNA and its N-terminal Sg and C-terminal Sg DNA were cloned into PET-100 vector. Positive colonies were screened and transformed into E. Coli BL21 (DE3). Recombinant Sg and its peptides were induced and expressed in high competent E. coli BL21 (DE3) , and purified by 50% Ni-NTA column. Inhibition activity assay was done by adding 4 different concentrations of semenogelin and its two peptides, 0, 1, 5 and 10 ng/microl, to human spermatozoa.</p><p><b>RESULTS</b>The peptide of Semenogelin that inhibits the activity of human spermatozoa was located in its N-terminal fragment. C-terminal Sg did not inhibit the activity of spermatozoa.</p><p><b>CONCLUSION</b>N-terminal Sg is the inhibition peptide of the whole molecular Sg. During semen liquefaction, this peptide should be cut off from the surface of human spermatozoa before they move forward.</p>


Subject(s)
Cloning, Molecular , Dose-Response Relationship, Drug , Escherichia coli , Genetics , Humans , Recombinant Proteins , Pharmacology , Seminal Vesicle Secretory Proteins , Genetics , Pharmacology , Sperm Motility
17.
National Journal of Andrology ; (12): 1080-1083, 2007.
Article in Chinese | WPRIM | ID: wpr-232010

ABSTRACT

<p><b>OBJECTIVE</b>To produce recombinant human prostate-specific antigen (PSA) by molecular cloning technology and to identify its activity.</p><p><b>METHODS</b>The human PSA cDNA and PET-12a vector were digested by NdeI and BamH1 before ligated by T4 ligase. The correct sequence was verified and transformed into high competent E. coli BL21 (DE3). Recombinant PSA was expressed and purified by hydrophobic interaction phenyl Sepharose column and activated by trypsin digestion. Enzymatic activation assay was done by hydrolysis of the substrate S-2586 and semenogelin.</p><p><b>RESULTS</b>Non-active recombinant PSA was digested by trypsin and demonstrated enzyme activity. The activated PSA hydrolyzed S-2586 and its physiological substrate semenogelin (Sg).</p><p><b>CONCLUSION</b>Recombinant pro-PSA can be an active serine protease by trypsin digestion and demonstrate native PSA enzymatic activity.</p>


Subject(s)
Blotting, Western , Cloning, Molecular , DNA, Complementary , Genetics , Escherichia coli , Genetics , Gene Expression , Humans , Hydrolysis , Male , Oligopeptides , Metabolism , Prostate-Specific Antigen , Genetics , Metabolism , Recombinant Proteins , Metabolism , Seminal Vesicle Secretory Proteins , Metabolism , Trypsin , Metabolism
18.
National Journal of Andrology ; (12): 168-170, 2007.
Article in Chinese | WPRIM | ID: wpr-297759

ABSTRACT

The epididymal protease inhibitor (Eppin) abounds in human semen and on the surface of human spermatozoa, specifically produced by the testis and epididymis. Recombinant Eppin has effected infertility in the immunized monkey and promises to be an effective vaccine for human immunocontraception. This article reviews the advances in the studies of Eppin gene and protein construction and its molecular mechanism of causing immunologic infertility and regulating PSA hydrolysis of Semenogelin.


Subject(s)
Animals , Humans , Infertility, Male , Allergy and Immunology , Male , Mice , Primates , Proteinase Inhibitory Proteins, Secretory , Chemistry , Genetics , Allergy and Immunology , Physiology , Seminal Vesicle Secretory Proteins , Physiology , Vaccines, Contraceptive
19.
National Journal of Andrology ; (12): 498-501, 2007.
Article in Chinese | WPRIM | ID: wpr-297697

ABSTRACT

<p><b>OBJECTIVE</b>To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.</p><p><b>METHODS</b>VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.</p><p><b>RESULTS</b>The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.</p><p><b>CONCLUSION</b>VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.</p>


Subject(s)
Blotting, Western , Gene Expression , Humans , Male , Polymerase Chain Reaction , Recombinant Proteins , Signal Transduction , Physiology , Spermatozoa , Metabolism , Testis , Metabolism , Voltage-Dependent Anion Channels , Physiology
20.
National Journal of Andrology ; (12): 636-638, 2007.
Article in Chinese | WPRIM | ID: wpr-297667

ABSTRACT

Seminal vesical secretion is important for male fertility. It affects semen coagulation, sperm motility, stability of sperm chromatin and suppression of the immune activity in the female reproductive tract.


Subject(s)
Female , Fertility , Physiology , Genitalia, Female , Allergy and Immunology , Humans , Male , Semen , Cell Biology , Seminal Vesicles , Allergy and Immunology , Physiology , Bodily Secretions , Sperm Count , Sperm Motility
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