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1.
Rev. Assoc. Med. Bras. (1992) ; 68(1): 37-43, Jan. 2022. tab, graf
Article in English | LILACS | ID: biblio-1360694

ABSTRACT

SUMMARY OBJECTIVE: This study aimed to develop and validate a practical nomogram to predict the occurrence of post-traumatic hydrocephalus in patients who have undergone decompressive craniectomy for traumatic brain injury. METHODS: A total of 516 cases were enrolled and divided into the training (n=364) and validation (n=152) cohorts. Optimal predictors were selected through least absolute shrinkage and selection operator regression analysis of the training cohort then used to develop a nomogram. Receiver operating characteristic, calibration plot, and decision curve analysis, respectively, were used to evaluate the discrimination, fitting performance, and clinical utility of the resulting nomogram in the validation cohort. RESULTS: Preoperative subarachnoid hemorrhage Fisher grade, type of decompressive craniectomy, transcalvarial herniation volume, subdural hygroma, and functional outcome were all identified as predictors and included in the predicting model. The nomogram exhibited good discrimination in the validation cohort and had an area under the receiver operating characteristic curve of 0.80 (95%CI 0.72-0.88). The calibration plot demonstrated goodness-of-fit between the nomogram's prediction and actual observation in the validation cohort. Finally, decision curve analysis indicated significant clinical adaptability. CONCLUSION: The present study developed and validated a model to predict post-traumatic hydrocephalus. The nomogram that had good discrimination, calibration, and clinical practicality can be useful for screening patients at a high risk of post-traumatic hydrocephalus. The nomogram can also be used in clinical practice to develop better therapeutic strategies.


Subject(s)
Humans , Decompressive Craniectomy/adverse effects , Brain Injuries, Traumatic/surgery , Brain Injuries, Traumatic/complications , Hydrocephalus/surgery , Hydrocephalus/etiology , Hydrocephalus/epidemiology , Cohort Studies , Nomograms
2.
Acta cir. bras ; 37(3): e370301, 2022. graf
Article in English | LILACS-Express | LILACS, VETINDEX | ID: biblio-1374079

ABSTRACT

ABSTRACT Purpose: Spontaneous intracerebral hemorrhage (ICH) is a major public health problem with a huge economic burden worldwide. Ulinastatin (UTI), a serine protease inhibitor, has been reported to be anti-inflammatory, immune regulation, and organ protection by reducing reactive oxygen species production, and inflammation. Necroptosis is a programmed cell death mechanism that plays a vital role in neuronal cell death after ICH. However, the neuroprotection of UTI in ICH has not been confirmed, and the potential mechanism is unclear. The present study aimed to investigate the neuroprotection and potential molecular mechanisms of UTI in ICH-induced EBI in a C57BL/6 mouse model. Methods: The neurological score, brain water content, neuroinflammatory cytokine levels, and neuronal damage were evaluated. The anti-inflammation effectiveness of UTI in ICH patients also was evaluated. Results: UTI treatment markedly increased the neurological score, alleviate the brain edema, decreased the inflammatory cytokine TNF-α, interleukin‑1β (IL‑1β), IL‑6, NF‑κB levels, and RIP1/RIP3, which indicated that UTI-mediated inhibition of neuroinflammation, and necroptosis alleviated neuronal damage after ICH. UTI also can decrease the inflammatory cytokine of ICH patients. The neuroprotective capacity of UTI is partly dependent on the MAPK/NF-κB signaling pathway. Conclusions: UTI improves neurological outcomes in mice and reduces neuronal death by protecting against neural neuroinflammation, and necroptosis.

3.
Rev. bras. med. esporte ; 27(spe): 31-33, Mar. 2021. tab, graf
Article in English | LILACS | ID: biblio-1156137

ABSTRACT

ABSTRACT The impact of traditional public health emergencies on the comprehensive education of medical students in colleges and universities is mainly reflected in the test of comprehensive literacy. Based on this, this paper studies the construction of a public health emergency impact analysis platform from a medical perspective and cloud computing. From the platform's database construction, event collection methods, impact evaluation rules and other aspects to achieve accurate analysis of the impact of emergencies, using the cloud computing method for comprehensive analysis and evaluation, the algorithm can analyze and intelligently classify information data on the Internet in the process of multiple input, and respond to potential public health emergencies according to cloud computing technology, in order to analyze the impact on the comprehensive quality of medical students. The experimental results show that the public health emergency analysis platform has the advantages of high feasibility and high data utilization, and can effectively improve the impact of public health emergencies on the comprehensive literacy of medical students.


RESUMO O impacto das tradicionais emergências de saúde pública sobre a formação integral de estudantes de medicina em faculdades e universidades reflete-se principalmente no teste de formação integral. Com base nisso, este documento estuda a construção da plataforma de análise de impacto de emergência de saúde pública sob a perspectiva médica e computação em nuvem. A partir da construção da base de dados da plataforma, foram desenvolvidos métodos de coleta de eventos, regras de avaliação de impacto e outros aspectos para obter uma análise precisa do impacto das emergências, usando o método de computação em nuvem para análise e avaliação. O algoritmo pode realizar a análise e classificação inteligente de dados de informação na Internet no processo de introdução múltipla, e responder a possíveis emergências de saúde pública de acordo com a tecnologia de computação em nuvem a fim de analisar o impacto sobre a qualificação dos estudantes de medicina. Os resultados experimentais mostram que a plataforma de análise de emergências de saúde pública tem as vantagens de alta viabilidade e alta utilização de dados, pode melhorar efetivamente o impacto das emergências de saúde pública na formação integral dos estudantes de medicina.


RESUMEN El impacto de las emergencias de salud pública tradicionales en la educación integral de los estudiantes de medicina en los colegios y universidades se refleja principalmente en la prueba de comprensión de textos. Con base en esto, este trabajo estudia la construcción de una plataforma de análisis de impacto de emergencias en salud pública desde una perspectiva médica y de computación en la nube. A partir de la construcción de la base de datos de la plataforma, los métodos de recolección de eventos, las reglas de evaluación de impacto y otros aspectos para lograr un análisis preciso del impacto de las emergencias, utilizando el método de computación en la nube para un análisis y evaluación integral, el algoritmo puede analizar y clasificar de manera inteligente los datos de información en Internet en el proceso de entrada múltiple. También puede responder a potenciales emergencias de salud pública de acuerdo con la tecnología de computación en la nube, con el fin de analizar el impacto en la calidad integral de los estudiantes de medicina. Los resultados experimentales muestran que la plataforma de análisis de emergencias de salud pública tiene las ventajas de alta viabilidad y alta utilización de datos, y puede mejorar de manera efectiva el impacto de las emergencias de salud pública en la comprensión de textos de los estudiantes de medicina.


Subject(s)
Humans , Medical Informatics , Biomedical Technology , Education, Medical , Emergency Medicine/education , Narrative Medicine , Algorithms
4.
Article in Chinese | WPRIM | ID: wpr-906695

ABSTRACT

@#[摘 要] 目的:评价活化自体淋巴细胞过继性免疫治疗(adoptive immunotherapy,AIT)是否有助于改善原发性肝细胞癌的临床疗效。方法:选取2016年8月至2018年12月在中国人民解放军总医院第五医学中心确诊的64例原发性肝细胞癌患者,通过分层随机法分为免疫治疗组(n=29)和对照组(n=35)。免疫治疗组患者取60 ml外周血分离制备单个核细胞并在含OKT-3和IL-2的培养基中活化培养,回输前进行质控检测。免疫治疗组中的Ⅰ~Ⅲ期患者(n=14)于一线治疗后接受自体淋巴细胞输注(3个月内输注6次),Ⅳ期患者(n=15)仅接受自体淋巴细胞输注;对照组患者接受肝细胞癌相关的其他治疗。疗效评估的主要终点是2年无复发生存(relapse-free survival,RFS)率,次要终点为无进展生存期(progression-free survival,PFS)和总生存期(overall survival,OS)。结果:入组患者中位随访时间为2.8年(0.2~4.2年)。免疫治疗组29名患者共接受了167次(计划174次,完成率96%)预定淋巴细胞输注(平均每人次回输9.30×109个细胞,其中CD3+HLA-DR细胞约占63%),治疗期间未观察到3级或4级不良反应发生。与对照组相比,免疫治疗组患者2年RFS率显著升高(62.1% vs 22.9%,OR=0.181,95%CI:0.06~0.54,P=0.002),中位PFS(28 vs 8个月,P=0.004)和中位OS(38 vs 34个月,P=0.915)均显著延长。在Ⅰ~Ⅲ期患者中,免疫治疗组(n=14)2年RFS率较对照组(n=18)显著升高(92.9% vs 33.3%,OR=0.38,95%CI:0.004~0.368,P=0.005),中位PFS明显延长(38 vs 14.5个月,P=0.005),而两组OS间无显著差异;Ⅳ期患者两组间PFS(P=0.077)及OS(P=0.994)均未见显著差异。结论:活化自体淋巴细胞AIT为安全可行的肝细胞癌辅助性治疗方法,可提高Ⅰ~Ⅲ期肝细胞癌一线治疗后RFS率、延长患者RFS时间,而对进展期肝细胞癌患者的PFS和OS无明显影响。

5.
Chinese Journal of School Health ; (12): 1423-1425, 2021.
Article in Chinese | WPRIM | ID: wpr-904564

ABSTRACT

Objective@#To explore the associated factors in the implementation of the "random" sampling inspection of the lighting institutions in kindergartens, outofschool training institutions and primary and secondary schools in 2019 (Supervision Letter [2019] No. 314 of National Health Office) (hereinafter referred to as the "Plan"), and to provide reference for better implementation of the Plan.@*Methods@#A survey of schoolbased myopia prevention and control was conducted in 23 provincial, municipal and countylevel health supervision institutions in China. The main contents of the survey focused on the introduction, implementation and implementation of the program.@*Results@#The implementation of the "plan" was significantly associated with the specific work organized and coordinated by the health administrative department, clear responsibility of various departments, and collaboration with educational institutions[OR(95%CI)=1.57(1.05-2.36), 0.05(0.03-0.10), 0.31(0.19-0.52), 0.03(0.01-0.09), 0.12(0.04-0.37), P<0.05]. It was also associated with independent school health supervision department, one and two fulltime school health supervision staff equipment, onsite quick inspection of health supervision institutions, school selfexamination[OR=1.87(1.33-2.62), 0.62(0.40-0.97), 2.37(1.82-3.09), 1.62(1.09-2.40), P<0.05].@*Conclusion@#The program needs to be implemented through multiple departments and is associated with multiple factors. It is of great importance to strengthen multisector collaboration and clarify the responsibility of various departments for the health supervision of schoolbased myopia prevention and control.

6.
Chinese Journal of School Health ; (12): 1093-1096, 2021.
Article in Chinese | WPRIM | ID: wpr-886350

ABSTRACT

Objective@#To understand the Implementation of Comprehensive Evaluation of School Health (GB/T 18205-2012) and associated factors, so as to provide rationalization proposals for future revision of standards.@*Methods@#A questionnaire survey was conducted on 436 schools, 56 health supervision agencies and 55 disease control agencies in Liaoning Province, and the main contents include the investigation of awareness rate, training, application, rationality and application of evaluation items and indicators.@*Results@#Totally 44 supervision agencies and 29 CDCs had independent school health departments, with significant differences in faculty equipment ( t =8.53, P <0.05). The standard was trained in 100% of municipal supervision agencies, 22.50% of district and county level, 46.15% of municipal CDC ,50.00% of district county CDC; 61.54% of municipal and 45.00% of district and county supervision agencies conducted evaluations in accordance with this standard, 53.85% of the municipal CDC, 60.00% of county CDC jointly completed the standard; 30.77% of municipal and 52.50% of district and county supervisory bodies informed the educational administration of the results of the comprehensive evaluation.@*Conclusion@#The establishment of school health professional departments and the training of management objects affect the implementation of comprehensive evaluation of school health, so it is necessary to combine the actual work to modify some of the important indicators, and strengthen the application of comprehensive evaluation results.

7.
Journal of Preventive Medicine ; (12): 111-116, 2021.
Article in Chinese | WPRIM | ID: wpr-876093

ABSTRACT

Objective@#To evaluate the effects of dietary behaviors on the risk of hypertension, diabetes and cardiovascular diseases.@*Methods@#A total of 12 208 subjects aged 18-60 years old were investigated by questionnaires to collect demographic data, dietary behaviors and lifestyle information, when they did health examination in a tertiary hospital in Beijing from 2014 to 2019. During the observation period of five year, the incidence of hypertension, diabetes and cardiovascular diseases were collected through health examination files every year. The multivariate logistic regression model was employed to analyze the associations of dietary behaviors with hypertension, diabetes and cardiovascular diseases. @*Results@#The study included 6 218 ( 50.93% ) males and 5 990 ( 49.07% ) females. The cumulative incidence rates of hypertension, diabetes and cardiovascular diseases were 7.75%, 2.72% and 3.49%, respectively. The multivariate logistic regression analysis indicated that the high-sodium diet ( OR=1.422, 95%CI: 1.191-1.697 ) , eating fast ( OR=1.457, 95%CI: 1.102-1.974 ), eating more refined grain ( OR=1.251, 95%CI: 1.050-1.490 ) and drinking milk less than once a week ( OR=1.316, 95%CI: 1.022-1.697 ) were risk factors for hypertension. The high-sodium diet ( OR=1.344, 95%CI: 1.048-1.725 ), eating fast ( OR=1.733, 95%CI: 1.046-2.871 ), eating more meat ( OR=1.651,95%CI: 1.263-2.158 ) were risk factors for diabetes. High-sodium diet ( OR=1.501, 95%CI: 1.192-1.889 ) was risk factors for cardiovascular disease. @*Conclusion@#The diet with high sodium, more meat and refined grain as well as eating fast can increase the risk of hypertension, diabetes and cardiovascular diseases.

8.
Chinese Journal of School Health ; (12): 106-108, 2021.
Article in Chinese | WPRIM | ID: wpr-862606

ABSTRACT

Objective@#To explore the correlation between BMI and gut microbiota of college students in Inner Mongolia,and to provide a reference basis for revealing the relationship between intestinal flora and obesity.@*Methods@#Totally 88 college students from Inner Mongolia Medical University were enrolled, Height and weight were measured,and the feces samples were collected. The bacterial metagenome was extracted from dry feces samples for the concentration detection in per gram of dry feces,expressed as μg/μL. Correlation between BMI and metagenomics concentration of gut microbiota was statistically analyzed. Meanwhile,the metagenomics concentration of gut microbiota in different BMI groups was compared with each other.@*Results@#There was a negative correlation between BMI and the metagenomics concentration of gut microbiota(r=-0.27,P<0.05). Significant difference in the concentration of gut microflora was observed between the normal group and the obesity group,the normal group and the overweight/obesity group(F=3.62,P<0.05). Among the female volunteers,there were significant differences between normal group and overweight group,between normal group and obesity group(F=1.87,P<0.05). No significant differences in metagenomics concentration of gut microbiota were found in different BMI groups(F=0.60, P>0.05).@*Conclusion@#There is a correlation between BMI and gut microbiota of college students in Inner Mongolia,the concentration of gut microflora metagenome in overweight and obese people decreased significantly.

9.
Article in English | WPRIM | ID: wpr-827467

ABSTRACT

OBJECTIVE@#To observe the changes of ischemic myocardial cells apoptosis in rats following intervention with Xuefu Zhuyu Oral Liquid (, XFZY), as well as changes of protein expression of silent information regulator 1 (SIRT1) and SIRT1 pathway-related genes.@*METHODS@#H9c2 rat myocardial cells were divided into 6 groups: control group, oxygen glucose deprivation (OGD) group, SIRT1 siRNA group, OGD+SIRT1 siRNA group, OGD+XFZY group, and OGD+SIRT1 siRNA+XFZY group. Quantitative fluorescent polymerase chain reaction (PCR) and Western blot were used to detect the concentration variations of SIRT1 and its pathway-related genes and corresponding protein expression after XFZY intervention and SIRT1 transfection.@*RESULTS@#Compared with the control group, the mRNA and protein expressions of SIRT1 were decreased obviously, while the mRNA and protein levels of P53, FoxO1, FoxO3, FoxO4 and nuclear factor kappa B (NF-ΚB) were increased in the OGD group, SIRT1 siRNA group, and OGD+SIRT1 siRNA group (P<0.01). Compared with the OGD group and OGD+SIRT1 siRNA group, the treatment of XFZY inhibited the decline in SIRT1 mRNA and protein expressions (P<0.01), and down-regulated the mRNA and protein levels of P53, FoxO1, FoxO3, FoxO4 and NF-ΚB, respectively (P<0.05 or P<0.01).@*CONCLUSION@#XFZY could prevent myocardial cells apoptosis probably by increasing the mRNA and protein expressions of SIRT1 and inhibiting the mRNA and protein expressions of P53, NF- K B, FoxO1, FoxO3 and FoxO4.

10.
Braz. j. med. biol. res ; 53(11): e9551, 2020. graf
Article in English | LILACS, ColecionaSUS | ID: biblio-1132485

ABSTRACT

The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared with the untreated asthma group. Similarly, treatment with M. vaccae inhibited Jagged1 expression and γδT17 cell production, which was associated with decreased airway inflammation and reactivity. The Notch signaling pathway may play a role in the pathogenesis of asthma through the induction of Jagged1 receptor. On the other hand, the inhibitory effect of M. vaccae on Jagged1 receptor in γδT17 cells could be used for the prevention and treatment of asthma.


Subject(s)
Animals , Rabbits , Signal Transduction , Mycobacterium , Ovalbumin , Receptors, Notch , Jagged-1 Protein
11.
Braz. arch. biol. technol ; 62: e19180021, 2019. tab, graf
Article in English | LILACS | ID: biblio-1055397

ABSTRACT

Abstract Clavibacter michiganensis subsp. michiganensis (Cmm), which is a Gram positive bacterium, causes the bacterial canker of tomato disease. The purpose of the study was to evaluate the antibacterial activity of Polygonum orientale extracts against Cmm. In this study, firstly, effects of three extracting parameters (extractive time, extractive temperature, and solid to liquid ratio) of orthogonal experiment design L27 (313) were conducted. Secondly, survival rate was determined and inhibition zone of Cmm rescued post-stress was monitored. Finally, extracellular OD260nm value, extracellular protein content, conformational structure of membrane protein, extracellular alkaline phosphatase (AKP) activity, and ATPase activity were measured to investigate the antibacterial mechanism. Results of orthogonal experiment revealed that extractive time and extractive temperature had highly significant (P<0.01) effects on the antibacterial activity of P. orientale extracts. The optimum conditions were as follows: 10h of extractive time, 60°C of extractive temperature, and 1:20 (g:mL) of solid to liquid ratio. This study also demonstrated that the living cells of each sample from survival rate test had almost no resistance or adaptability, and rescued Cmm cells were much easier to be inhibited by P. orientale extracts. The results of antibacterial mechanism indicated that cell membrane and cell wall of Cmm were seriously damaged by P. orientale extracts, and P. orientale extracts reduced the intracellular ATPase activity dramatically. All these findings suggested that P. orientale extracts had a strong antibacterial activity to inhibit Cmm, and could be used for the ecological management of the bacterial canker of tomato disease.


Subject(s)
Plant Diseases , Pest Control, Biological , Phytochemicals , Anti-Bacterial Agents
12.
Braz. j. med. biol. res ; 52(10): e8845, 2019. tab, graf
Article in English | LILACS | ID: biblio-1039251

ABSTRACT

Regucalcin is a soluble protein that is principally expressed in hepatocytes. Studies of regucalcin have mainly been conducted in animals due to a lack of commercially available kits. We aimed to develop an enzyme-linked immunosorbent assay (ELISA) to quantify serum regucalcin in patients with hepatitis B virus (HBV)-related disease. High-titer monoclonal antibodies and a polyclonal antibody to regucalcin were produced, a double-antibody sandwich ELISA method was established, and serum regucalcin was determined in 47 chronic hepatitis B (CHB) patients, 91 HBV-related acute-on-chronic liver failure (HBV-ACLF) patients, and 33 healthy controls. The ELISA demonstrated an appropriate linear range, and high levels of reproducibility, sensitivity, specificity, accuracy, and stability. The median serum regucalcin concentrations in HBV-ACLF and CHB patients were 5.46 and 3.76 ng/mL, respectively (P<0.01), which were much higher than in healthy controls (1.72 ng/mL, both P<0.01). For the differentiation of CHB patients and healthy controls, the area under curve (AUC) was 0.86 with a cut-off of 2.42 ng/mL, 85.7% sensitivity, and 78.8% specificity. In contrast, the AUC of alanine aminotransferase (ALT) was lower (AUC=0.80, P=0.01). To differentiate ACLF from CHB, the AUC was 0.72 with a cut-off of 4.26 ng/mL, 77.0% sensitivity, and 61.2% specificity while the AUC of ALT was 0.41 (P=0.07). Thus, we have developed an ELISA that is suitable for measuring serum regucalcin and have shown that serum regucalcin increased with the severity of liver injury due to HBV-related diseases, such that it appears to be more useful than ALT as a marker of liver injury.


Subject(s)
Humans , Male , Female , Adolescent , Adult , Middle Aged , Aged , Young Adult , Calcium-Binding Proteins/blood , Hepatitis B, Chronic/blood , Renal Insufficiency/blood , Severity of Illness Index , Enzyme-Linked Immunosorbent Assay/methods , Biomarkers/blood , Case-Control Studies , Reproducibility of Results , Sensitivity and Specificity , Hepatitis B, Chronic/virology , Renal Insufficiency/virology , Antibodies, Viral/immunology
13.
Braz. j. med. biol. res ; 52(7): e7374, 2019. tab, graf
Article in English | LILACS | ID: biblio-1011596

ABSTRACT

This study aimed to investigate the association of serum high-mobility group box-1 (HMGB1) and toll-like receptor 4 (TLR4) expressions with the risk of epilepsy as well as their correlations with disease severity and resistance to anti-epilepsy drugs. One hundred and five epilepsy patients and 100 healthy controls (HCs) were enrolled in this case-control study, and serum samples were collected from all participants to assess the HMGB1 and TLR4 expressions by enzyme-linked immunosorbent assay (ELISA). Both serum HMGB1 (P<0.001) and TLR4 (P<0.001) expressions were higher in epilepsy patients than in HCs, and they displayed good predictive values for risk of epilepsy. Moreover, HMGB1 was positively correlated with TLR4 level (r=0.735, P<0.001). HMGB1 and TLR4 levels were both elevated in patients with an average seizure duration >5 min compared to patients with a seizure duration ≤5 min (P=0.001 and P=0.014, respectively). Also, HMGB1 and TLR4 were increased in patients with seizure frequency >3 times per month compared to patients with seizure frequency ≤3 times per month (both P=0.001). In addition, HMGB1 and TLR4 expressions were higher in intractable cases compared to drug-responsive cases (P<0.001). In conclusion, both HMGB1 and TLR4 expressions were correlated with increased risk and severity of epilepsy and their level was higher in patients resistant to anti-epilepsy drugs.


Subject(s)
Humans , Male , Female , Adult , HMGB1 Protein/blood , Epilepsy/blood , Toll-Like Receptor 4/blood , Anticonvulsants/therapeutic use , Severity of Illness Index , Enzyme-Linked Immunosorbent Assay , Case-Control Studies , Predictive Value of Tests , Risk Factors , Epilepsy/drug therapy
14.
Biol. Res ; 52: 41, 2019. tab, graf
Article in English | LILACS | ID: biblio-1019505

ABSTRACT

BACKGROUND: Di-N-butyl-phthalate (DBP) is an endocrine disrupting substance. We investigated the adverse effect of DBP on testis of male rat and reveal its potential mechanism of MAPK signaling pathway involved this effect in vivo and in vitro. Gonadal hormone, sperm quality, morphological change and the activation status of JNK, ERK1/2 and p38 was determined in vivo. Primary Sertoli cell was established and cultivated with JNK, ERK1/2 inhibitors, then determine the cell viability, apoptosis and the expression of p-JNK, p-ERK1/2. Data in this study were presented as mean ± SD and determined by one-way analysis of variance (ANOVA) followed by Bonferroni's test. Difference was considered statistically significant at P < 0.05. RESULTS: In vivo experiment, DBP impaired the normal structure of testicular tissue, reduced testosterone levels in blood serum, decreased sperm count and increased sperm abnormality, p-ERK1/2 and p-JNK in rat testicular tissue increased in a dose-dependent manner. In vitro studies, DBP could decrease the viability of Sertoli cells and increase p-ERK1/2 and p-JNK. Cell apoptosis in SP600125 + DBP group was significantly lower than in DBP group (P < 0.05). p-JNK was not significantly decreased in SP600125 + DBP group, while p-ERK1/2 was significantly decreased in U0126 + DBP group. CONCLUSIONS: These results suggest that DBP can lead to testicular damage and the activation of ERK1/2 and JNK pathways, the JNK signaling pathway may be primarily associated with its effect.


Subject(s)
Animals , Male , Rats , Testis/injuries , Testis/metabolism , Signal Transduction/physiology , Mitogen-Activated Protein Kinases/metabolism , MAP Kinase Signaling System/physiology , JNK Mitogen-Activated Protein Kinases/metabolism , Dibutyl Phthalate/pharmacology , Testis/drug effects , Rats, Sprague-Dawley , Mitogen-Activated Protein Kinases/physiology , JNK Mitogen-Activated Protein Kinases/physiology
15.
Article in Chinese | WPRIM | ID: wpr-777769

ABSTRACT

Objective@#To investigate the osteogenic properties of maxillary sinus membrane stem cells (MSMSCs). @*Methods @#Beagle maxillary sinus mucosa was collected, immunomagnetic bead method was applied for isolation of CD146+ cells, and MSMSCs were harvested and cultured from the canine maxillary sinus floor mucosa. The levels of the cell surface antigens CD44, CD146, and CD34 were determined at passage one by flow cytometry. Cells at passage one were cultured in basal medium and osteogenic inductive medium. Real-time PCR, immunohistochemical staining, alkaline phosphatase activity, alizarin red staining and Von Kossa staining were used to investigate the osteogenic properties in vitro. @*Results@#The canine MSMSCs were cultured successfully. The results of flow cytometry were positive for CD146 and CD44 expression but negative for CD34 expression. The relative mRNA expression of runt-related transcription factor 2 (RUNX2) (t = 14.44,P < 0.001), osteopontin (OPN) (t = 7.85,P = 0.001) and alkaline phosphatase alkaline phosphatase (t = 14.27,P < 0.001) was apparently higher in the osteoinductive medium group than in the basal medium group, the differences in relative mRNA expression between the groups were significant. The protein levels of RUNX2 and OPN increased in the osteoinductive medium group. The alkaline phosphatase activity of the MSMSCs increased when the cells were cultured in osteoinductive medium; the activity increased to a level that was significantly higher than that in basal medium, particularly at days 3 (t = 8.79, P < 0.001), 7 (t = 9.75,P < 0.001), 14 (t = 12.14,P < 0.001), 21 (t = 19.62,P < 0.001) and 28 (t = 17.53,P < 0.001). Obvious mineralized nodules were observed by alizarin red staining or Von Kossa staining.@*Conclusion @#Maxillary sinus membrane stem cells exhibit osteogenic ability.

16.
Article in Chinese | WPRIM | ID: wpr-777739

ABSTRACT

Objective@#To detect the expression level of miR-27a during the osteogenic differentiation of beagle maxillary sinus membrane stem cells (MSMSCs) and explore the role of miR-27a in the osteogenic differentiation of MSMSCs.@*Methods@#Beagle MSMSCs were cultured in vitro. The expression level of miR-27a was detected via RT-PCR after an osteogenic inductive culture was prepared. The mRNA expression levels of Runx2 and OPN were examined via RT-PCR, and the protein expression levels of Runx2 and OPN were examined via Western blot after the cells were transfected with pre-miR-27a or anti-miR-27a. Finally, osteoprogenitor cells transfected with pre-miR-27a were composited with Bio-Oss particles and subcutaneously implanted into nude mice to form ectopic bone formation models, and then the inhibition of bone formation from miR-27a was observed in vivo. @*Results@#The expression level of miR-27a in the beagle MSMSCs decreased after osteogenic inductive culturing. The relative miR-27a levels were significantly decreased at day 1 (t=3.795, P=0.023), day 3 (t=4.493, P=0.011), day 7 (t=11.591, P < 0.001), day 14 (t=12.542, P < 0.001), and day 21 (t=5.621, P=0.008) compared with day 0. In addition, the expression levels of Runx2 mRNA (t=4.923, P=0.007) and protein (t=4.425, P=0.008) were reduced after the cells were transfected with pre-miR-27a. The expression levels OPN mRNA (t=5.253, P=0.006) and protein (t=5.132, P=0.006) were also reduced. In contrast, the mRNA expression levels of Runx2 (t=3.925, P=0.013) and OPN (t=3.712, P=0.019) were increased after the cells were transfected with anti-miR-27a, and bone formation was observed after the subcutaneous implantation of beagle MSMSCs composited with Bio-Oss in nude mice. Nevertheless, ectopic bone formation was inhibited by pre-miR-27a-transfected beagle MSMSCs composited with Bio-Oss (t=7.219, P=0.0020). @* Conclusion @# MiR-27a negatively regulates the osteogenic differentiation of MSMSCs.

17.
Article in Chinese | WPRIM | ID: wpr-801636

ABSTRACT

@# Objective: To study the effects of kaempferol on invasion and migration of non-small cell lung cancer (NSCLC)A549 cells and the related mechanisms. Methods: CCK-8 was used to detect the effect of different concentrations of kaempferol on the proliferation of A549 cells. Transwell assay and wound healing assay were used to detect the ability of cell invasion and migration. The expressions of EMT-related proteins (N-cadherin, Snail-2 and E-cadherin) were detected by Western blotting. The effect of kaempferol on the mRNA and protein expressions of estrogen related receptor alpha (ERRα) was determined by qRT-PCR. ERRα over-expression vector (pLV-ERRα) was transfected intoA549 cells, and the cell invasion, migration and the expression of EMT related proteins were detected by Transwell assay, wound healing assay and Western blotting, respectively. Results: Kaempferol dose-dependently inhibited the proliferation of A549 cells, and kaempferol at concentrations of 5, 10 and 20 μmol/L was used in the following experiments. After the treatment with different concentrations of kaempferol, the number of invasive cells and wound closure rate, the expression of N-cadherin and Snail-2, and the mRNAand protein levels of ERRα decreased significantly, while the expression of E-cadherin increased significantly, (all P<0.01). After transfection with pLV-ERRα, the number of invasive cells, scratch closure rate and the expressions of N-cadherin and Snail-2 of A549 cells over-expressing ERRα significantly increased, while the expression of E-cadherin decreased significantly (all P<0.05 or P<0.01); and all these indices were attenuated after the treatment with kaempferol (all P<0.05 or P<0.01). Conclusion: Kaempferol inhibits the invasion and migration as well as EMT of non-small cell lung cancer A549 cells by down-regulating ERRα, which may provide experimental basis for the clinical treatment of lung cancer.

18.
Electron. j. biotechnol ; 29: 68-77, sept. 2017. tab, graf
Article in English | LILACS | ID: biblio-1017370

ABSTRACT

Background: Platycladus orientalis has an extremely long life span of several thousands of years, attracting great interests in the mechanisms involved in such successful senescence regulation and resistance at physiological and molecular levels. Results: The levels of reactive oxygen species (ROS) were higher in 3,000-year-old than in 20-year-old P. orientalis, and the activities of GR and GSH demonstrated the same trend. We produced and analyzed massive sequence information from pooled samples of P. orientalis through transcriptome sequencing, which generated 51,664 unigenes with an average length of 475 bp. We then used RNA-seq analysis to obtain a high-resolution age­course profile of gene expression in 20- and 3,000-year-old P. orientalis individuals. Totally, 106 differentially expressed genes were obtained, of which 47 genes were downregulated and 59 upregulated in the old tree. These genes were involved in transcription factors, hormone-related responses, ROS scavengers, senescence-related responses, stress response, and defense and possibly play crucial roles in tackling various stresses in the 3,000-year-old P. orientalis during its life time. The expression patterns of genes related to ROS homeostasis further indicated that the high ability of ROS scavenging could be helpful for the 3,000-year-old P. orientalis to resist senescence. Conclusions: This study provides a foundation for the elucidation of senescence resistance through molecular studies and the discovery of useful genes in P. orientalis.


Subject(s)
Aging/genetics , Cupressaceae/genetics , Transcriptome , Gene Expression Regulation , Free Radical Scavengers , Sequence Analysis, RNA , Reactive Oxygen Species , Reverse Transcriptase Polymerase Chain Reaction , Homeostasis
19.
Electron. j. biotechnol ; 25: 33-38, ene. 2017. tab, ilus
Article in English | LILACS | ID: biblio-1008414

ABSTRACT

Background: Banana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Conventional breeding has been seriously hampered by their long generation time, polyploidy, and sterility of most cultivated varieties. Establishment of an efficient regeneration and transformation system for banana is critical to its genetic improvement and functional genomics. Results: In this study, a vigorous and repeatable transformation system for banana using direct organogenesis was developed. The greatest number of shoots per explant for all five Musa varieties was obtained using Murashige and Skoog medium supplemented with 8.9 µM benzylaminopurine and 9.1 µM thidiazuron. One immature male flower could regenerate 380­456, 310­372, 200­240, 130­156, and 100­130 well-developed shoots in only 240­270 d for Gongjiao, Red banana, Rose banana, Baxi, and Xinglongnaijiao, respectively. Longitudinal sections of buds were transformed through particle bombardment combined with Agrobacterium-mediated transformation using a promoterless ß-glucuronidase (GUS) reporter gene; the highest transformation efficiency was 9.81% in regenerated Gongjiao plantlets in an optimized selection medium. Transgenic plants were confirmed by a histochemical assay of GUS, polymerase chain reaction, and Southern blot. Conclusions: Our robust transformation platform successfully generated hundreds of transgenic plants. Such a platform will facilitate molecular breeding and functional genomics of banana.


Subject(s)
Musa/growth & development , Musa/genetics , Regeneration , Transformation, Genetic , Immunohistochemistry , Blotting, Southern , Polymerase Chain Reaction , Plants, Genetically Modified , Agrobacterium tumefaciens/physiology , Musa/microbiology , Organogenesis, Plant , Glucuronidase
20.
Psicol. reflex. crit ; 30: 17, 2017. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-955749

ABSTRACT

Abstract We used the virtual hand illusion paradigm to study how sense of agency and sense of (body) ownership can interact with different emotional events on stress feelings. Converging evidence for at least the partial independence of agency and ownership was found. For instance, sense of agency was a better predictor of individual anxiety levels than sense of ownership and males showed stronger effects related to agency—presumably due to gender-specific attribution styles and empathy skills. Moreover, agency and ownership also interacted with emotional events and led to different anxiety levels. Taken together, our findings suggest that the disentangled sense of agency and sense of ownership can interact with different emotional events and influenced stress feelings more in threatening situations than awarding ones.

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