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1.
Article in Chinese | WPRIM | ID: wpr-908071

ABSTRACT

Objective:Visual analysis of the current research status, hotspots and frontier of spiritual care will provide reference for further research on spiritual and develop Spiritual care for patients with chronic diseases in China in the future.Methods:Using CNKI, Wanfang and VIP database as the data source, this paper uses Bibliometric method and CiteSpace software to carry out visual analysis from five aspects such as annual volume of literature, author of literature, institution of literature and high-frequency keywords.Results:A total of 167 literatures were included, and the volume of publications showed an increasing trend; the distribution of institutions and regions was uneven; the sources of journals were scattered; the cooperation between authors and institutions was lacking, the research hot spots and frontier are "spiritual care needs", "cancer patients", "spiritual health", "spiritual intervention" and "nurse's spiritual care ability".Conclusions:The research of spiritual care for chronic patients in China started late and developed slowly, and the depth and breadth of research need to be strengthened. In the future, we should strengthen the diversified scientific research cooperation and exchanges across authors, institutions and regions, draw on the experience and lessons of foreign research, building a theoretical system of spiritual care for patients with chronic diseases suitable for China's national conditions, and promote the scientific, diversified and high-quality development of spiritual care for patients with chronic diseases in China.

2.
Journal of Experimental Hematology ; (6): 1595-1600, 2021.
Article in Chinese | WPRIM | ID: wpr-922301

ABSTRACT

OBJECTIVE@#To investigate the procedure of pre-transfusion testing and transfusion strategy of patients with multiple myeloma (MM) treated by daratumumab (DarA).@*METHODS@#The blood samples of MM patients before and after DarA treatment from the Fifth Affiliated Hospital of Sun Yat-sen University were collected, and the ABO/Rh blood group antigen identification and DAT test results were compared. The results of antibody screening and cross matching of the patients before and after inactivation of red blood cells with 0.2 mol/L dithiothreitol (DTT) were compared and analyzed.@*RESULTS@#ABO/Rh blood group antigen typing showed no affecting in patients after treated by DarA; the result of DAT test showed negative. Irregular antibody screening showed that all the three cells(Ⅰ, Ⅱ and Ⅲ) were positive(1+~2+) and the self-control was negative. By microcolumn agglutination method, the main side of the multi-bag of blood showed no matched, while the secondary side showed all identical. After treated by DTT solution, the cross matching results in reagent red blood cells and the red blood cells of blood donors were both consistent, and the irregular antibody screening was negative. The K(+)O type erythrocytes used in parallel control were transformed into K(-)O type erythrocytes after DTT treatment. However, there was no significant changes in E(+) O type erythrocytes before and after DTT treatment. There was no condensation on the primary and secondary side of the condensed amine method. The primary and secondary sides of blood matching by saline method showed negative.@*CONCLUSION@#After treated by DarA, cross matching results from microcolumn agglutination method can be interfered by the residual drug antibody in MM patients, while the interference was eliminated in the presence of 0.2 mol/L DTT solution. However, no disturbance was observed when using condensed amine method or saline method. Therefore, corresponding transfusion procedures should be selected according to the emergency degree of blood transfusion to ensure the safety and timeliness of blood transfusion.


Subject(s)
Antibodies, Monoclonal , Blood Transfusion , Dithiothreitol , Humans , Multiple Myeloma/therapy
3.
Chinese Journal of Lung Cancer ; (12): 756-763, 2021.
Article in Chinese | WPRIM | ID: wpr-922143

ABSTRACT

BACKGROUND@#Segmentectomy has gradually become one of the standard surgical methods for small pulmonary nodules with early lung cancer on imaging. This study aimed to investigate the perioperative outcomes of patients who underwent uniport video-assisted thoracoscopic surgery (VATS) segmentectomy for identifying the intersegmental boundary line (IBL) by the near-infrared fluorescence imaging with intravenous indocyanine green (ICG) method or the modified inflation-deflation (MID) method and assess the feasibility and effectiveness of the ICG fluorescence (ICGF)-based method.@*METHODS@#We retrospectively analyzed the perioperative data in total 198 consecutive patients who underwent uniport VATS segmentectomy between February 2018 and August 2020. With the guidance of preoperative intelligent/interactive qualitative and quantitative analysis-three dimensional (IQQA-3D), the targeted segment structures could be precisely identified and dissected, and then the IBL was confirmed by ICGF-based method or MID method. Clinical effectiveness and postoperative complications of the two methods were evaluated.@*RESULTS@#An IBL was visible in 98% of patients by the ICGF-based group, even with the low-doses of ICG. The ICGF-based group was significantly associated with the shorter IBL clear presentation time [(23.59±4.47) s vs (1,026.80±318.34) s] (P0.05).@*CONCLUSIONS@#The ICGF-based method could highly accurately identify the IBL and make anatomical segmentectomy easier and faster, and therefore has the potential to be a feasible and effective technique to facilitate the quality of uniport VATS segmentectomy.


Subject(s)
Feasibility Studies , Humans , Indocyanine Green , Ion Transport , Lung Neoplasms/surgery , Pneumonectomy , Postoperative Complications , Retrospective Studies , Thoracic Surgery, Video-Assisted
4.
Article in Chinese | WPRIM | ID: wpr-887905

ABSTRACT

Discoidin domain receptor 1(DDR1)is a critical member of the receptor tyrosine kinase family.It may be related to tumor invasion and metastasis,and the abnormal activation of DDR1 can lead to the occurrence and development of malignant tumors,inflammation,and fibrosis.DDR1 are involved in cell adhesion,migration,proliferation,secretion of cytokines,and remodeling of extracellular matrix,thus playing a critical role in various pathophysiological processes of the human body.In this review,we demonstrate the research progress of DDR1 in breast cancer and other malignant tumors,in order to provide a new theoretical basis for the prevention and treatment of breast cancer and other tumors.


Subject(s)
Breast Neoplasms/genetics , Cell Adhesion , Discoidin Domain Receptor 1 , Female , Fibrosis , Humans , Receptor Protein-Tyrosine Kinases/genetics
5.
Chinese Medical Journal ; (24): 1584-1592, 2021.
Article in English | WPRIM | ID: wpr-887592

ABSTRACT

BACKGROUND@#There were few studies on real-world data about autologous hematopoietic stem cell transplantation (auto-HSCT) or allogeneic HSCT (allo-HSCT) in peripheral T-cell lymphoma (PTCL). This study aimed to investigate the clinical outcomes of patients who received auto-HSCT or allo-HSCT in China.@*METHODS@#From July 2007 to June 2017, a total of 128 patients who received auto-HSCT (n  = 72) or allo-HSCT (n  = 56) at eight medical centers across China were included in this study. We retrospectively collected their demographic and clinical data and compared the clinical outcomes between groups.@*RESULTS@#Patients receiving allo-HSCT were more likely to be diagnosed with stage III or IV disease (95% vs. 82%, P = 0.027), bone marrow involvement (42% vs. 15%, P = 0.001), chemotherapy-resistant disease (41% vs. 8%, P = 0.001), and progression disease (32% vs. 4%, P < 0.001) at transplantation than those receiving auto-HSCT. With a median follow-up of 30 (2-143) months, 3-year overall survival (OS) and progression-free survival (PFS) in the auto-HSCT group were 70%(48/63) and 59%(42/63), respectively. Three-year OS and PFS for allo-HSCT recipients were 46%(27/54) and 44%(29/54), respectively. There was no difference in relapse rate (34%[17/63] in auto-HSCT vs. 29%[15/54] in allo-HSCT, P = 0.840). Three-year non-relapse mortality rate in auto-HSCT recipients was 6%(4/63) compared with 27%(14/54) for allo-HSCT recipients (P = 0.004). Subanalyses showed that patients with lower prognostic index scores for PTCL (PIT) who received auto-HSCT in an upfront setting had a better outcome than patients with higher PIT scores (3-year OS: 85% vs. 40%, P = 0.003). Patients with complete remission (CR) undergoing auto-HSCT had better survival (3-year OS: 88% vs. 48% in allo-HSCT, P = 0.008). For patients beyond CR, the outcome of patients who received allo-HSCT was similar to that in the atuo-HSCT group (3-year OS: 51% vs. 46%, P = 0.300).@*CONCLUSIONS@#Our study provided real-world data about auto-HSCT and allo-HSCT in China. Auto-HSCT seemed to be associated with better survival for patients in good condition (lower PIT score and/or better disease control). For patients possessing unfavorable characteristics, the survival of patients receiving allo-HSCT group was similar to that in the auto-HSCT group.


Subject(s)
China , Hematopoietic Stem Cell Transplantation , Humans , Lymphoma, T-Cell, Peripheral/therapy , Neoplasm Recurrence, Local , Retrospective Studies , Transplantation, Autologous , Transplantation, Homologous , Treatment Outcome
6.
Article in Chinese | WPRIM | ID: wpr-883953

ABSTRACT

Objective:To explore the influence of childhood abuse experience on relapse tendency of compulsory drug abusers, and the intermediary role of resilience and depression between them.Methods:A total of 261 drug abusers were investigated with childhood trauma questionnaire(CTQ), the Connor-Davidson resilience scale (CD-RISC), self-rating depression scale (SDS) and relapse tendency psychological questionnaire.All data processing and analysis were carried out by SPSS 25.0 software.Correlation analysis, Bootstrap analysis and other methods were used to test the mediating effect.Results:The scores of childhood abuse treatment, resilience, depression and relapse tendency were 44.690±18.550, 60.210±22.790, 48.370±12.450, and 15.300±9.833 respectively.Childhood abuse was negatively correlated with resilience( r=-0.396, P<0.01), and positively correlated with depression and relapse tendency( r=0.584, 0.298, P<0.01).Resilience was negatively correlated with depression and relapse tendency( r=-0.558, -0.147, P<0.05).Depression had a significant positive correlation with relapse tendency( r=0.286, P<0.01).Childhood abuse could directly predict the tendency of relapse( β=0.202, P<0.01), and it could also affect the tendency of relapse through the mediation of depression( β=0.082, 95% CI=0.007-0.165) and the chain mediation of resilience and depression( β=0.029, 95% CI=0.002-0.064). Conclusion:The experience of childhood abuse leads to the decrease of resilience of strong abstinence personnel and increases the level of depression, which finally increases the tendency of relapse.

7.
Article in Chinese | WPRIM | ID: wpr-880176

ABSTRACT

OBJECTIVE@#To investigate the changes in function of CD8@*METHODS@#Flow cytometry was used to detect the expressions of PD-1, TIM-3, and LAG-3, which were the markers of exhausted CD8@*RESULTS@#The expressions of inhibitory receptors (PD-1, TIM3 and LAG-3) on CD8@*CONCLUSION@#The exhausted CD8


Subject(s)
CD8-Positive T-Lymphocytes , Hepatitis A Virus Cellular Receptor 2 , Humans , Interferon-gamma , Lymphocyte Count , Lymphohistiocytosis, Hemophagocytic
8.
Article in Chinese | WPRIM | ID: wpr-877077

ABSTRACT

Objective To analyze the global status of COVID-19 epidemics, so as to preliminarily forecast the epidemic trend. Methods The epidemiological data of 208 countries and the prevention and control policies implemented by typical countries from December 31, 2019 to December 14, 2020 were collected. We use the cumulative incidence rate, cumulative mortality, cumulative fatality and real-time dependent reproduction number (Rt) to analyze the epidemic status. We use the provenance package to group different countries and discuss the effect of prevention and control measures. Results As of December 14, 2020, a cumulative incidence of 93.49 per 10000, a cumulative mortality rate of 0.21‰, and a cumulative fatality rate of 3.1‰ had been reported globally.112 of the 208 countries still had Rt ≥ 1.0, and 96 countries had Rt t , and the government had adopted more relaxed epidemic prevention measures. The epidemic situation in this region may continue to deteriorate, and needs to be focused in the later period.

9.
China Pharmacy ; (12): 1235-1240, 2021.
Article in Chinese | WPRIM | ID: wpr-876892

ABSTRACT

OBJECTIVE:To establish the fingerprint of Jingu tongxiao pill ,and to determine the contents of 7 components. METHODS:HPLC method was adopted. The determination was performed on Cosmosil 5C18-MS-Ⅱ column with acetonitrile- 0.02% phosphoric acid solution as mobile phase (gradient elution )at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm and the column temperature was set at 25 ℃. The sample size was 10 μL. HPLC fingerprint of 10 batches of Jingu tongxiao pill was established by using Similarity Evaluation System of TCM Chromatographic Fingerprint (2012 edition),and common peak was identified by comparing with the mixed reference substance. The contents of corresponding components of the identified common peak were determined by the same HPLC method. RESULTS :There were 20 common peaks in HPLC fingerprint of 10 batches of samples ,and the similarity with the control fingerprint was not less than 0.980. By comparing with the mixed reference substance, 7 components were identified , which were loganic acid ,gentiopicroside,paeoniflorin,salvianolic acid B , cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ A. The linear range of the above 7 components were 4.509-45.090, 15.090-150.900,14.985-149.850,14.982-149.820,2.967-29.670,1.944-19.440,3.094-30.940 μg/mL(all r>0.999),respectively. The limits of detection were 0.060 1,0.161 0,0.399 6,0.159 8,0.031 6,0.051 8,0.082 5 μg/mL,respectively. The limits of quantitation were 0.200 4,0.503 0,0.999 0,0.399 5,0.079 1,0.259 2,0.412 6 μg/mL,respectively. RSDs of precision ,stability (24 h) and reproducibility tests were all lower than 3.0% (n=6). Average recoveries were 98.81% -100.28% ,RSDs were 0.20%-1.21%(n=6). In 10 batches of samples ,the contents of the above 7 components were 0.441 0-0.969 4,3.283 4-4.733 4, 1.947 7-3.674 9,1.336 6-2.270 9,0.293 2-0.372 1,0.190 2-0.293 9 and 0.352 8-0.518 8 mg/g,respectively. CONCLUSIONS :In this study,HPLC fingerprint and content determination method of Jingu tongxiao pill are successfully established and can be used for quality control.

10.
Article in Chinese | WPRIM | ID: wpr-888236

ABSTRACT

As a low-load physiological monitoring technology, wearable devices can provide new methods for monitoring, evaluating and managing chronic diseases, which is a direction for the future development of monitoring technology. However, as a new type of monitoring technology, its clinical application mode and value are still unclear and need to be further explored. In this study, a central monitoring system based on wearable devices was built in the general ward (non-ICU ward) of PLA General Hospital, the value points of clinical application of wearable physiological monitoring technology were analyzed, and the system was combined with the treatment process and applied to clinical monitoring. The system is able to effectively collect data such as electrocardiogram, respiration, blood oxygen, pulse rate, and body position/movement to achieve real-time monitoring, prediction and early warning, and condition assessment. And since its operation from March 2018, 1 268 people (657 patients) have undergone wearable continuous physiological monitoring until January 2020, with data from a total of 1 198 people (632 cases) screened for signals through signal quality algorithms and manual interpretation were available for analysis, accounting for 94.48 % (96.19%) of the total. Through continuous physiological data analysis and manual correction, sleep apnea event, nocturnal hypoxemia, tachycardia, and ventricular premature beats were detected in 232 (36.65%), 58 (9.16%), 30 (4.74%), and 42 (6.64%) of the total patients, while the number of these abnormal events recorded in the archives was 4 (0.63%), 0 (0.00%), 24 (3.80%), and 15 (2.37%) cases. The statistical analysis of sleep apnea event outcomes revealed that patients with chronic diseases were more likely to have sleep apnea events than healthy individuals, and the incidence was higher in men (62.93%) than in women (37.07%). The results indicate that wearable physiological monitoring technology can provide a new monitoring mode for inpatients, capturing more abnormal events and provide richer information for clinical diagnosis and treatment through continuous physiological parameter analysis, and can be effectively integrated into existing medical processes. We will continue to explore the applicability of this new monitoring mode in different clinical scenarios to further enrich the clinical application of wearable technology and provide richer tools and methods for the monitoring, evaluation and management of chronic diseases.


Subject(s)
Heart Rate , Humans , Monitoring, Physiologic , Movement , Sleep Apnea Syndromes , Wearable Electronic Devices
11.
Chinese Journal of Hematology ; (12): 117-122, 2020.
Article in Chinese | WPRIM | ID: wpr-799578

ABSTRACT

Objective@#To explore the efficacy and prognostic factors of hematopoietic stem cell transplantation (HSCT) for the treatment of patients with anaplastic large cell lymphoma (ALCL) .@*Methods@#The clinical records of 33 ALCL patients after HSCT were collected and analyzed retrospectively to evaluate the rates of overall survival (OS) and recurrence after autologous (auto-HSCT) and allogeneic HSCT (allo-HSCT) and the factors influencing prognosis.@*Results@#The median-age of this cohort of 33 ALCL cases at diagnosis was 31 (12-57) years old with a male/female ratio of 23/10, 24 cases (72.7%) were ALK+ and 9 ones (27.3%) ALK-. Of them, 25 patients (19 ALK+ and 6 ALK-) underwent auto-HSCT and 8 cases (5 ALK+ and 3ALK-) allo-HSCT with a median follow-up of 18.7 (4.0-150.0) months. Disease states before HSCT were as follows: only 6 patients achieved CR status and received auto-HSCT, 16 patients achieved PR (14 cases by auto-HSCT and 2 ones allo-HSCT) , the rest 11 cases were refractory/relapse (5 cases by auto-HSCT and 6 ones allo-HSCT) . There were 7 cases died of disease progression (5 after auto-HSCT and 2 allo-HSCT) and 5 cases treatment-related mortality (TRM) (2 after auto-HSCT and 3 allo-HSCT) , TRM of two groups were 8.0% and 37.5%, respectively. Both the median progression-free survival (PFS) and OS were 15 months after auto-HSCT, the median PFS and OS after allo-HSCT were 3.7 (1.0-90.0) and 4.6 (1.0-90.0) months, respectively. There was no statistically significant difference in terms of survival curves between the two groups (OS and PFS, P=0.247 and P=0.317) . The 2-year OS rates in auto-HSCT and allo-HSCT groups were 72% and 50%, respectively. The 5-year OS rates in auto-HSCT and allo-HSCT groups were 36% and 25%, respectively.@*Conclusion@#ALCL treated by chemotherapy produced high rates of overall and complete responses. Chemotherapy followed by auto-HSCT remained to be good choice for patients with poor prognostic factors. High-risk patients should be considered more beneficial from allo-HSCT.

12.
Article in Chinese | WPRIM | ID: wpr-863225

ABSTRACT

Objective:To explore the relationship between the selection of different P2Y 12 inhibitors and the long-term prognosis of acute coronary syndrome (ACS) patients with and without CYP2C19 defect gene. Method:289 consecutive ACS patients who underwent percutaneous coronary intervention (PCI) at Tianjin Third Central Hospital from March 2016 to October 2016 were selected for CYP2C19 gene polymorphism detection. According to the detection results, the patients were divided into group A (with CYP2C19 loss-of-function gene, 199 cases) and group B (without CYP2C19 loss-of-function gene, 90 cases). After PCI, different P2Y 12 inhibitors were selected. The patients were followed up for 3 years, and 23 cases were lost to follow-up. Finally, 182 cases were enrolled in group A and 84 cases were enrolled in group B. According to whether there were major adverse cardiovascular events (MACE) within 3 years, the patients in groups A and B were divided into MACE subgroups (58 cases, 32 cases) and non-MACE subgroups (124 cases, 52 cases). The single factor analysis of the two subgroups in groups A and B was carried out based on the patient's clinical data, coronary artery disease and intervention status, and postoperative drug treatment plan. Risk factors with statistical significance ( P<0.05) were selected, and multivariate logistic regression analysis was performed on groups A and B to compare the effects of different P2Y 12 inhibitors on the prognosis of the two groups. Results:The differences in platelet volume, fasting blood glucose, HbA1c, left ventricular end-diastolic diameter, proportion of single-branch lesions, proportion of intervention for left main lesions, and dual antiplatelet therapy were statistically significant between the two subgroups in group A (all P<0.05). The differences in low-density lipoprotein (LDL), left ventricular ejection fraction (LVEF), left ventricular end-diastolic diameter, proportion of two-branch lesions, proportion of three-branch lesions, and proportion of using tirofeben were statistically significant between the two subgroups in group B (all P<0.05). In the group A, the choice of different P2Y 12 inhibitors was the independent risk factor for the long-term prognosis. Compared with patients treated with Ticagrelor, the probability of long-term MACE was 11.971 times larger ( OR=12.971, 95% CI: 5.028~33.464, P<0.001) among patients treated with Clopidogrel 75 mg/day, and 5.029 times larger ( OR=6.029, 95%CI: 2.278~15.958) among patients treated with Clopidogrel 100 mg/day. No significant correlation was witnessed between different P2Y 12 inhibitors and long-term prognosis in group B. In the group B, different P2Y 12 inhibitors have no significant correlation with their long-term prognosis of patients( P>0.05). Conclusions:For ACS patients with CYP2C19 loss-of-function gene, the choice of P2Y 12 inhibitors is associated with their long-term MACE events after PCI. Ticagrelor therapy brings the lowest risk of long-term MACE. For those without CYP2C19 loss-of-function gene, the correlation between the choice of different P2Y 12 inhibitors and their prognosis is not significant.

13.
Journal of Leukemia & Lymphoma ; (12): 605-609, 2020.
Article in Chinese | WPRIM | ID: wpr-862895

ABSTRACT

Objective:To analyze the clinical characteristics of chronic granulomatous disease (CGD) with chronic active EB virus (EBV) infection (CAEBV).Methods:The clinical data of 1 CGD patient combined with CAEBV who underwent allogeneic hematopoietic stem cell transplantation (HSCT) in Beijing Friendship Hospital of Capital Medical University in March 2017 was retrospectively analyzed, and the relevant literature was also summarized.Results:After transplantation, the chimeric rate and gene test was 100% complete donor type. During the follow-up period, no recurrent severe infection occurred within 39 months, and EBV-DNA copy was 0; no acute or chronic graft-versus-host disease (GVHD) related manifestations occurred, and the quality of life was higher.Conclusions:Haploid allogeneic HSCT with myeloablative conditioning regimens is a relatively safe and effective treatment for CGD patients combined with CAEBV. For primary immunodeficiency disease, it suggests that the haploidentical donor carrying the monohybrid pathogenic gene can also be used as an effective stem cell source if the hematological and immunological function is normal.

14.
Chinese Pharmaceutical Journal ; (24): 631-636, 2020.
Article in Chinese | WPRIM | ID: wpr-857732

ABSTRACT

OBJECTIVE: To establish the HPLC fingerprint of Jinyinhua formula granules (JYHFG), and compare the qualities of the products from nine enterprises by chemical metrology analysis. METHODS: HPLC was used to establish fingerprint at 254 nm. Twenty-nine batches of products from nine enterprises were determined, and the quality of JYHFG of different batches was evaluated through similarity evaluation, clustering analysis (HCA) and principal component analysis (PCA). RESULTS: Seventeen common peaks were confirmed in the HPLC fingerprint of 29 batches of JYHFG, and 10 of them were identified. The similarity of JYHFG was 0.84-0.99, and the samples could be grouped into one category according to enterprise, among which the characteristic peak of chlorogenic acid had the greatest influence on the fingerprint. CONCLUSION: The establishment of HPLC fingerprint and the identification of chemical pattern provide a more comprehensive reference for the quality control of JYHFG. It provides a reliable method for scientific evaluation and effective quality control.

15.
Article in Chinese | WPRIM | ID: wpr-847420

ABSTRACT

BACKGROUND: Masquelet technique is one of the effective methods to repair large bone defects. This technique requires the use of more autologous bone in the second stage of surgical treatment. However, the source of autologous bone is limited and the complications in the donor area are inevitable. How to reduce the use of autologous bone is an urgent problem to be solved. OBJECTIVE: By combining with the development status of induced membrane technology at home and abroad, this review introduced some effective methods to reduce the amount of autologous bone in induced membrane technology, including filling bone graft replacement materials and some special surgical methods. METHODS: The first author used a computer to retrieve the literature published in PubMed, CNKI and Wanfang databases from January 1996 to September 2019. The search terms were “Masquelet technique; induced membrane; bone transport technique; autologous bone; bone defect; bone graft; 3D printing; tissue engineering”. RESULTS AND CONCLUSION: Since the emergence of the Masquelet technique, the technique has been constantly improved and innovated by scholars. However, there is no international consensus on how to reduce the use of autologous bone in the second stage of Masquelet technique. At present, each of improvement methods has its own advantages and disadvantages, and clinicians need to choose according to objective conditions. The method proposed by Jong-Keon Oh, using gelfoam as the bone grafting center and the peripheral ring for bone grafting, is simple and practical. Tissue engineering technology has potential for development. With the further study of seed cells and scaffold materials, it will gradually replace the existing treatment schemes. According to the existing research, the research direction of Masquelet technique in the future can be roughly summarized into four directions: The improvement of membrane technology, the improvement of surgical methods, the application of combined materials, and the application of 3D printing and tissue engineering technology. These directions need further exploration and development by scholars.

16.
Article in Chinese | WPRIM | ID: wpr-845975

ABSTRACT

Objective: To determine the total saponins from Gynostemma pentaphyllum, the dammarane-type triterpenoids of its hydrolysate, and its hypoglycemic activity. Methods: Compounds from the acid hydrolyzate extracts and total saponins were isolated by silica gel, recrystal and preparative liquid chromatography, and their structures were identified by the NMR spectral analysis. The sensitive screening modles of α-glucosidase and PTP1B inhibitors were established in vitro. The inhibitory kinetics of compounds were also investigated. Using the method of computer aided drug design of active site, PTP1B interact with the strongest active compound for docking simulation. Results: Seven compounds were isolated from the acid hydrolyzate of total saponins, which identified as gpsapogenin A (1), 20(S)-panaxadiol (2), gypensapogenin F (3), 20(R)-protopanaxadiol (4), (23S)-3β- hydroxydama-20,24-diene-21-carboxylic acid 21,23-lactone (5), gypsapogenin A (6), and (20S,24S)-3β,20,21β,23β,25- pentahydroxy-21,24-epoxydammarane (7). Five compounds were isolated from total saponins, including (20R,23R)- 3β,20-dihydroxydammar-24-en-21-oic acid 21,23-lactone 3-O-[α-L-rhamnopyranosyl(1→2)][β-D-xylopyranosyl(1→3)]-6-O- acetyl-β-D-glucopyranoside (8), (20S,23S)-3β,20-dihydroxydammar-24-en-21-oic acid 21,23-lactone 3-O-[α-L-rhamnopyranosyl(1→2)][β-D-xylopyranosyl(1→3)]-6-O-acetyl-β-D-glucopyranoside (9), (20R,23R)-19-oxo-3β,20-dihydroxydammar-24-en-21-oci acid 21,23-lactone3-O-[α-L-rhamnopyranosyl-(1→2)][β-D-xylopyranosyl(1→3)]-α-L-arabinopyranoside (10), (20S)-3β,20,21- trihydroxydammar-23,25-diene 3-O-{[α-L-rhamnopyranosyl(1→2)][β-D-xylopyranosyl(1→3)]-β-D-glucopyranosyl}-21-O-β- D-glucopyranoside (11), and (20S,23S)-3β,20-dihydroxydammar-24-en-21-oic acid and 21,23-lactone 3-O-[α-L-rhamnopyranosyl(1→2)][β-D-xylopyranosyl-(1→3)]-β-D-glucopyranoside (12). Conclusion: Beside compound 4, the other compounds showed inhibitory activity against α-glucosidase and PTP1B. For the α-glucosidase and PTP1B inhibitions assay, compound 9 indicated the strongest inhibitory effect with IC50 2.10 and 1.07 μmol/L, respectively.

17.
Chinese Medical Journal ; (24): 1805-1814, 2020.
Article in English | WPRIM | ID: wpr-827921

ABSTRACT

BACKGROUND@#We previously found that the intestinal epithelial chemokine (C-C motif) ligand 7 (CCL7) plays an important role in the development of toxin-induced acute liver damage. The detailed effects of intestinal epithelial CCL7 on chronic diseases; however, are still unclear. Here, we aimed to investigate the impact of intestinal epithelial CCL7 overexpression on high-fat diet (HFD)-induced obesity and steatohepatitis in mice.@*METHODS@#Intestinal epithelial CCL7 overexpression (CCL7) mice and their wild-type (WT) littermates were fed with normal chow or HFD for 16 weeks to induce obesity and non-alcoholic fatty liver disease. Body weight gain, as well as adipose tissue index were assessed. Liver injury was monitored by histological analysis and real time polymerase chain reaction. Gut microbial composition was analyzed by 16S rRNA gene sequencing.@*RESULTS@#We found that the CCL7 mice on a HFD had markedly decreased weight gain (8.9 vs. 17.0 g, P < 0.05) and a lower adipose tissue index that include mesenteric fat (1.0% vs. 1.76%, P < 0.05), gonadal fat (2.1% vs. 6.1%, P < 0.05), subcutaneous fat (1.0% vs. 2.8%, P < 0.05) compared to WT animals. HFD-induced glucose intolerance and insulin resistance were also significantly improved in CCL7 mice compared to WT. Furthermore, HFD-fed CCL7 mice displayed less hepatic lipid accumulation and lower expression of inflammatory factors than WT mice. 16S rRNA gene sequencing demonstrated that CCL7 overexpression in intestinal epithelial cells improved HFD-induced gut microbial dysbiosis.@*CONCLUSIONS@#Our study revealed that CCL7 overexpression in the intestinal epithelium protects mice against the progression of diet-induced obesity, hepatic steatosis, and enteric dysbiosis.

18.
Journal of Experimental Hematology ; (6): 1376-1380, 2020.
Article in Chinese | WPRIM | ID: wpr-827109

ABSTRACT

OBJECTIVE@#To establish a secondary hemophagocytic lymphohistiocytosis(HLH) mouse model, and to investigate the effect of ruxolitinib on the disease manifestation of model mice.@*METHODS@#Wild type C57BL/6 mice were randomly divided into 4 groups: two groups of mice were intraperitoneally injected with CpG oligodeoxynucleotide 1826 (CpG-ODN1826) every other day to induce HLH, and other two groups were control groups. One group of the CpG-ODN1826 groups and one of the control groups were given ruxolitinib, and other two groups were given the same amount of PBS. Blood samples, serum ferritin and hepatic/spleen weights of experimental mice were detected and serum cytokine levels were measured by ELISA.@*RESULTS@#Compared with the control groups, the levels of white blood cells, hemoglobin and platelets in the CpG-ODN1826 groups were significantly lower (P<0.05); and liver/body weight, spleen/body weight, serum ferritin, sCD25, IL-10, IL-1β, IFN-Ƴ, IL-12p70, GM-CSF, TNF-α and IL-18 levels significantly increased (P<0.05). There was no significant difference in the levels of IL-2, IL-4, IL-5, IL-6, IL-22, IL-13, IL-27 and IL-23 between the two groups (P>0.05). The spleen in CpG group had disordered internal structure, expanding red pulp and hyperplastic nucleated cells. The liver had severe perivascular inflammations. The spleen/weight of the ruxolitinib-treated mice in the CpG-ODN1826 group was significantly smaller than that of the unapplied ruxolitinib (P<0.05).@*CONCLUSION@#The CpG-ODN1826 can induce secondary HLH symptoms in wild type C57BL/6 mice. Ruxolitinib can alleviate the symptoms of splenomegaly in HLH model mice.


Subject(s)
Animals , Disease Models, Animal , Lymphohistiocytosis, Hemophagocytic , Mice , Mice, Inbred C57BL , Pyrazoles
19.
Article in Chinese | WPRIM | ID: wpr-871367

ABSTRACT

Objective:To investigate the potential mechanism by which Brucella suis vaccine strain S2 induces the apoptosis of BV-2 microglia cells, and to discover new protein targets for neurobrucellosis treatment. Methods:BV-2 microglia cells were treated with Brucella suis vaccine strain S2 for 0, 3, 6, 12 and 24 h. Western blot assay and RT-qPCR were performed to detect the expression of p-JNK and p53 at protein and mRNA levels in BV-2 microglia cells. Cell apoptosis was measured by flow cytometry. Immunofluorescence was used to analyze nuclear p-JNK. Results:Brucella suis vaccine strain S2 could promote the expression of p-JNK and p53 at both protein and mRNA levels and increase nuclear p-JNK in BV-2 microglia cells. Moreover, it could also induce the apoptosis of BV-2 microglia cells. Conclusions:Brucella suis vaccine strain S2 could promote the apoptosis of BV-2 microglia cells through activating JNK and promoting p53 expression.

20.
Article in Chinese | WPRIM | ID: wpr-773720

ABSTRACT

To evaluate the pesticide residue risk of Jinyinhua Formula Granules( made from Lonicerae Japonicae Flos) used in the market preliminarily,20 samples of Jinyinhua Formula Granules from 5 manufactures were collected randomly through the national evaluative sampling test program. Totally 262 pesticides( involving 270 chemical monomers) with monitoring significance to traditional Chinese medicinal materials were detected. Samples were extracted by high speed homogenate with acetonitrile as solvent. And their residues were analyzed by GC-MS/MS and LC-MS/MS in MRM mode. No less than 2 groups of characteristic ion pairs were adopted for qualitative detection,and the calibration curve method was used for quantitative detection. The results showed that 20 pesticides were detected in 20 batches of Jinyinhua Formula Granules,with an average of about 9 pesticides detected in every batch,but no restricted pesticide was detected. The detected pesticides were all at the trace level,which was far lower than the limit of the general food standard. Therefore,the safety risk was low in Jinyinhua Formula Granules. In this study,a screening method for pesticide residues in Jinyinhua Formula Granules was established for the first time. The method was accurate and rapid,and the detection indicators were highly targeted. The results could provide theoretical reference for the prevention and control of pesticide safety risks in Jinyinhua Formula Granules and even traditional Chinese medicine formula granules.


Subject(s)
Chromatography, Gas , Chromatography, Liquid , Drug Contamination , Drugs, Chinese Herbal , Pesticide Residues , Quality Control , Tandem Mass Spectrometry
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