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National Journal of Andrology ; (12): 596-600, 2011.
Article in Chinese | WPRIM | ID: wpr-305838


<p><b>OBJECTIVE</b>To analyze the relation between recurrent miscarriage and routine semen parameters.</p><p><b>METHODS</b>We compared the semen parameters of normal healthy men with those of the spouses of recurrent miscarriage women through 1: 1 age-matched case-control study.</p><p><b>RESULTS</b>Compared with the healthy controls, the men of the case group showed a significantly lower mean semen volume ([1.95 +/- 1.11] ml vs [2.74 +/- 1.43] ml), sperm concentration ([48.68 +/- 20.07] x 10(6)/ml vs [59.26 +/- 25.35] x 10(6)/ml), percentage of grade b sperm ([12.07 +/- 3.34] % vs [16.18 +/- 6.74] %), fruit-sugar content ([1.73 +/- 0.64] g/L vs [2.21 +/- 0.75] g/L), acrosomal enzyme activity ([84.34 +/- 26.69] U/mg prot vs [94.20 +/- 26.35] U/mg prot), alpha-glucuronidase (alpha-GLU) content ([36.28 +/- 15.98] U/ml vs [44.45 +/- 12. 54] U/ml), and acid phosphatase (ACP) content ([68.55 +/- 35.45] U/ml vs [84.78 +/- 51. 10] U/ml) (P < 0.05), but remarkably higher percentages of head teratospermia ([47.36 +/- 4.59] % vs [46.50 +/- 6.32] %) and tail teratospermia ([7.56 +/- 2.27] % vs [7.28 +/- 3.10] %), and elastase content ([885.64 +/- 1 272.30] ng/ml vs [661.08 +/- 764.64] ng/ml) (P < 0.05). Based on the results of discriminant analysis, the semen volume, percentages of grade b sperm and combined teratospermia, and contents of fruit-sugar, alpha-GLU and ACP could be used to evaluate the semen and sperm quality of the spouses of recurrent miscarriage women.</p><p><b>CONCLUSION</b>Routine semen and sperm tests might help evaluate the seminal factors of recurrent miscarriage, but they lack specificity and need comprehensive analysis. Poorer semen quality is associated with higher incidence of recurrent miscarriage.</p>

Abortion, Habitual , Adult , Case-Control Studies , Female , Humans , Male , Pregnancy , Semen , Sperm Count , Sperm Motility , Spermatozoa
National Journal of Andrology ; (12): 1075-1079, 2007.
Article in Chinese | WPRIM | ID: wpr-232011


<p><b>OBJECTIVE</b>To study the infection of human cytomegalovirus (HCMV) and herpes simplex virus type II (HSV-I) and the morphological characteristics of the infected spermatogenic cells in the semen of infertile men.</p><p><b>METHODS</b>We washed and concentrated the spermatogenic cells obtained from 83 semen samples of infertile men, extracted DNA and then screened HCMV and HSV-II by polymerase chain reaction (PCR). Immunocytochemistry (ICC) was used to detect the expression of correlative virus antigens of the positive semen cells, and the cytology smear was employed to observe the morphological changes of the spermatogenic cells under the microscope after cytology staining.</p><p><b>RESULTS</b>Of all the semen samples, 8 were HCMV positive, 4 HSV-II positive, but none were both HCMV and HSV-II positive. HCMV late antigens were positively and HCMV early antigens negatively expressed in the spermatogenic cells of the 8 HCMV positive cases. In the 4 HSV-II positive cases, 3 were positively and 1 weakly positively expressed. In the semen of the 12 positive cases were found large numbers of immature spermatogenic cells, with different manifestations of apoptosis, such as chromatin pycnosis, vacuoles, damaged nuclear membrane, and apoptotic bodies, but without virus infection-induced specific morphological alteration. Sperm concentration of the positive group was significantly lower than that of the negative (P < 0. 05).</p><p><b>CONCLUSION</b>Spermatogenic cells infected by HCMV and HSV-II may cause pathologic lesions and affect spermatogenesis. Morphologically, the infected spermatogenic cells may undergo some pathologic alteration, such as apoptosis. The rate of HCMV infection is higher among infertile males with pathologic cells in the semen.</p>

Adult , Antigens, Viral , Cytomegalovirus , Genetics , Allergy and Immunology , Cytomegalovirus Infections , Pathology , Virology , DNA, Viral , Genetics , Herpes Simplex , Pathology , Virology , Herpesvirus 2, Human , Genetics , Allergy and Immunology , Humans , Immunohistochemistry , Infertility, Male , Pathology , Virology , Male , Polymerase Chain Reaction , Semen , Cell Biology , Virology , Spermatozoa , Cell Biology , Virology
National Journal of Andrology ; (12): 904-907, 2005.
Article in Chinese | WPRIM | ID: wpr-339398


<p><b>OBJECTIVE</b>To study the effects of dietetic hyperlipidemia on the development of testes and penises in male New Zealand rabbits.</p><p><b>METHODS</b>The animal model of dietetic hyperlipidemia was established by feeding 36 male New Zealand rabbits which were divided into the test group (n = 20) and the control group (n = 16) at random with rich fat. The concentration of TC, TG, HDL-C as well as LDL-C was determined by using automatic biochemistry analyzer. Radioimmunoassay methods were used to measure the level of T, LH and FSH as well. Alterations of testicular and penial development in morphology were observed by HE staining.</p><p><b>RESULTS</b>Compared with the control group, concentrations of TC, TG and LDL-C increased dramatically as well as the levels of T, LH and FSH decreased obviously. There are significant differences between the two groups (P <0.01). Compared with the control group, the penial length was short (P < 0.05) and testicular coefficient declined (P < 0.01) in the test group. With light microscope, the following phenomenon was manifestly detected: the sabotage of spermatogenic epithelium and the adipocyte sedimentation of the penial tissue.</p><p><b>CONCLUSION</b>It is obvious that intaking a large quantity of food with rich fat from juvenile would induce dietetic hyperlipidemia, result in the functional disorder of hypothalamo-pituitary-gonadal axis, and leads to short penis in development and the damage of spermatogenesis function as well.</p>

Animal Feed , Animals , Diet , Disease Models, Animal , Hyperlipidemias , Pathology , Lipids , Blood , Male , Penis , Pathology , Rabbits , Random Allocation , Testis , Pathology