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1.
Article in Chinese | WPRIM | ID: wpr-871367

ABSTRACT

Objective:To investigate the potential mechanism by which Brucella suis vaccine strain S2 induces the apoptosis of BV-2 microglia cells, and to discover new protein targets for neurobrucellosis treatment. Methods:BV-2 microglia cells were treated with Brucella suis vaccine strain S2 for 0, 3, 6, 12 and 24 h. Western blot assay and RT-qPCR were performed to detect the expression of p-JNK and p53 at protein and mRNA levels in BV-2 microglia cells. Cell apoptosis was measured by flow cytometry. Immunofluorescence was used to analyze nuclear p-JNK. Results:Brucella suis vaccine strain S2 could promote the expression of p-JNK and p53 at both protein and mRNA levels and increase nuclear p-JNK in BV-2 microglia cells. Moreover, it could also induce the apoptosis of BV-2 microglia cells. Conclusions:Brucella suis vaccine strain S2 could promote the apoptosis of BV-2 microglia cells through activating JNK and promoting p53 expression.

2.
Article in Chinese | WPRIM | ID: wpr-867832

ABSTRACT

Current classifications of tibial plateau fractures include three-pillar classification, four-quadrant classification, eight-segment classification, ten-segment classification, and four-column & nine-segment classification. This article reviews the various CT classifications of tibial plateau fractures, their advantages and disadvantages and surgical approaches as well. The essence of the CT classifications is to pay more attention to the coronary fracture line, especially the posterior coronal fracture fragments. A classification which combines the four-quadrant idea of the articular surface of the tibial plateau with the four-column idea of the peripheral cortex of the tibial plateau, and is supplemented by descriptions of non-articular surface structures (intercondylar spine, tibial tubercle and fibula head), may provide a more comprehensive understanding of a specific tibial plateau fracture, but may therefore be too complicated and difficult to use clinically due to too many combinations that need matching.

3.
Chinese Journal of Rheumatology ; (12): 312-316, 2016.
Article in Chinese | WPRIM | ID: wpr-670260

ABSTRACT

Objective To investigate the serum level of anti N-methyl-D-aspartate (NMDA) receptor antibody in patients with cognitive dysfunction of systemic lupus erythematosus (SLE) and its clinical significance.Methods The levels of anti NMDA receptor antibody in blood samples from 36 patients with cognitive dysfunction of SLE,30 cases with normal cognitive function of neuropsychiatric lupus erythematosus (NPSLE) groups,64 patients with no-NPSLE,100 patients with other connective tissue diseases and 30 healthy controls were measured by enzyme linked immunosorbent assay,and the reports of Mini-Mental State Examination and the laboratory indexes were recorded in those groups,then these index were measured again when patients were treated for 2 weeks.The comparison of multi-group was conducted by means of single factor analysis of variance or rank test,the Pearson or Spearman correlation analysis was used to analyze the correlation between variables.Results ① The level of anti NMDA receptor antibody in the patients with cognitive dysfunction of the NPSLE group (1.30±0.27) was higher than those with normal cognitive function of NPSLE group (0.47±0.08),No-NPSLE group (0.47±0.07),the other connective tissue disease (0.42±0.04) and the healthy controls (0.35±0.06) (P<0.05),the difference was statistically significant.The levels of anti NMDA receptor antibody before treatment were higher than those after the treatment,and the difference was statistically significant (t =2.296,P<0.05).② The serum levels of anti NMDA receptor antibody in patients were negatively correlated with the intelligent score (r=-0.330,P<0.01),and were positively correlated with the levels of IgG (r=0.684,P<0.05).Conclusion The anti NMDA receptor antibody can be used as a new autoantibody in the early diagnosis of cognitive dysfunction in patients with SLE,and can be used for monitoring the activity of disease in cognitive dysfunction of SLE.

5.
Chinese Critical Care Medicine ; (12): 294-299, 2014.
Article in Chinese | WPRIM | ID: wpr-465926

ABSTRACT

Objective To study the species and amount of bacteria in sputum of patients with ventilator associated pneumonia (VAP) by using 16S rDNA sequencing analysis,and to explore the new method for etiologic diagnosis of VAP.Methods Bronchoalveolar lavage sputum samples were collected from 31 patients with VAP.Bacterial DNA of the samples were extracted and identified by polymerase chain reaction (PCR).At the same time,sputum specimens were processed for routine bacterial culture.The high flux sequencing experiment was conducted on PCR positive samples with 16S rDNA macro genome sequencing technology,and sequencing results were analyzed using bioinformatics,then the results between the sequencing and bacteria culture were compared.Results ① 550 bp of specific DNA sequences were amplified in sputum specimens from 27 cases of the 31 patients with VAP,and they were used for sequencing analysis.103 856 sequences were obtained from those sputum specimens using 16S rDNA sequencing,yielding approximately 39 Mb of raw data.Tag sequencing was able to inform genus level in all 27 samples.② Alpha-diversity analysis showed that sputum samples of patients with VAP had significantly higher variability and richness in bacterial species (Shannon index values 1.20,Simpson index values 0.48).Rarefaction curve analysis showed that there were more species that were not detected by sequencing from some VAP sputum samples.③ Analysis of 27 sputum samples with VAP by using 16S rDNA sequences yielded four phyla:namely Acitinobacteria,Bacteroidetes,Firmicutes,Proteobacteda.With genus as a classification,it was found that the dominant species included Streptococcus 88.9% (24/27),Limnohabitans 77.8% (21/27),Acinetobacter 70.4% (19/27),Sphingomonas 63.0% (17/27),Prevotella 63.0% (17/27),Klebsiella 55.6% (15/27),Pseudomonas 55.6% (15/27),Aquabacterium 55.6% (15/27),and Corynebacterium 55.6% (15/27).④ Pyrophosphate sequencing discovered that Prevotella,Limnohabitans,Aquabacterium,Sphingomonas might not be detected by routine bacteria culture.Among seven species which were identified by both methods,pyrophosphate sequencing yielded higher positive rate than that of ordinary bacteria culture [Streptococcus:88.9% (24/27) vs.18.5% (5/27),KlebsieHa:55.6% (15/27) vs.18.5% (5/2 7),Acinetobacter:70.4% (19/27) vs.37.0% (10/27),Corynebacterium:55.6% (15/27) vs.7.4% (2/27),P<0.05 or P<0.01].Sequencing positive rate was found to increase positive rate for culture of Pseudomonas [55.6% (15/27) vs.25.9% (7/27),P=0.050].No significant differences were observed between sequencing and ordinary bacteria culture for detection Staphylococcus [7.4% (2/27) vs.11.1% (3/27)] and Neisseria bacteria genera [18.5% (5/27) vs.3.7%(1/27),both P>0.05].Conclusions 16S rDNA sequencing analysis confirmed that pathogenic bacteria in sputum of VAP were complicated with multiple drug resistant strains.Compared with routine bacterial culture,pyrophosphate sequencing had higher positive rate in detecting pathogens.16S rDNA gene sequencing technology may become a new method for etiological diagnosis of VAP.

6.
Article in Chinese | WPRIM | ID: wpr-747071

ABSTRACT

OBJECTIVE@#To evaluate the relationship between human papilloma virus (HPV) infection and the occurrence, recurrence and malignant transformation of nasal inverted papilloma (NIP).@*METHOD@#With comprehensive retrieval of related literature that had been published in databases included Pubmed (1990--2011), Cochrane Library, CNKI (1979-2011), VIP (1989-2011), CBM (1990-2011) and WANFANG Meta-analysis software Rev-Man 5.0 was used to analyze the raw data and to calculate the value of combined odd ratio (OR) and 95% confidence interval (CI).@*RESULT@#According to Meta-analysis, the occurrence group between HPV and NIP, the OR was 34.44 and 95% CI was 12.96-91.56; the recurrence group, the OR was 3.66 and 95% CI was 1.77-7.56, to the high-risk HPV, the OR was 1.94 and 95% CI was 0.30-12.58; the malignant transformation group, the OR was 1.79 and 95% CI was 0.94-3.40 to the high-risk HPV, the OR was 49.35 and 95% (CI was 0.45-11.23.@*CONCLUSION@#HPV may play an important role in the occurrence and recurrence of NIP and high-risk HPV was closely related to the progress of NIP.


Subject(s)
DNA, Viral , Humans , Nose Neoplasms , Virology , Papilloma, Inverted , Virology , Papillomaviridae , Papillomavirus Infections , Pathology
7.
Article in Chinese | WPRIM | ID: wpr-405924

ABSTRACT

Objective To investigate re-innervation in the neovaginal mucosa of patients underwent sigmoid colon vaginoplasty in treatment of Mayer-Rokitansky-Kistner-Hauser Syndrome (MRKHS).Methods Biopsies in the upper third of the posterior neovagina were taken in 20 patients treated by sigmoid colon vaginoplasty at 1, 2 and 3 years after surgery, respectively. Protein gene product 9. 5 ( PGP 9. 5 ),vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) were detected by immunohistochemical method and compared with those in intact sigmoid colon mucosa. Results ( 1 ) Density of nerve fiber:abundant distribution of PGP 9. 5 nerve fibers were observed in the mucosal muscle layer, submucosa, and smooth muscle layer of the neovagina. The nerve fibers of VIP and NPY immunoreactivity were mainly distributed around blood vessels and in the smooth muscles. In the neovagina, the density of nerve fibers of PGP 9. 5 of 17 ± 6 were much more than VIP of 2. 9 ± 1.0 and NPY of 2. 5 ± 0. 8 significantly ( P < 0. 05 ).( 2 ) Expression of PGP 9. 5 in neovagina: at 1 year after surgery, PGP 9. 5 positive expression of 14 ± 4 was significantly lower in the neovagina than 28 ± 7 in the intact sigmoid colon( P < 0. 05 ). However, after 2 to 3 years, its expression displayed an upgrade tendency in the neovagina and was significantly higher at the 3 year postoperatively than that at the 1 years postoperatively ( 22 ± 7 vs. 14 ± 4, P < 0. 05 ). The changes were much more obvious in submucosa. (3) The expression of VIP and NPY in neovagina: at 1 year after surgery, VIP and NPY positive nerve fibers were also decreased in the neovagina when compared with those in the intact sigmoid colon ( 2. 3 ± 0. 7 vs. 5.3 ± 1.4, P < 0. 05; 2.5 ± 1. 1 vs. 5.5 ± 1.1, P < 0. 05 ) . At 2 to 3 years after surgery, the positive VIP fiber showed initially decreased and subsequently increased tendency. The density of VIP of 3.7 ± 0. 7 in the neovagina at 3 years postoperatively was higher than 2. 3 ±0. 7 at 1 years postoperatively (P < 0. 05 ). No significant up-regulation was observed in NPY-positive expression in the neovagina within 3 years after operation. Conclusions Distribution of sensory PGP 9. 5,VIP and NPY immunoreactive nerve fibers was similar to the pattern observed within the intact sigmoid colon wall. The number of nerve fibers in the neovagina decreased after surgery and then increased subsequently within 3 years after surgery.

8.
Article in Chinese | WPRIM | ID: wpr-387567

ABSTRACT

Objective To investigate the prevalence of Borna disease virus (BDV) in viral encephalitis patients in Ningxia Hui Autonomous Region and to analyze phylogenetic development of BDV in this area. Methods The gene fragments of BDV were amplified by real time nested reverse transcriptase polymerase chain reaction (PCR) from peripheral blood mononuclear cells (PBMC) of 60viral encephalitis (VE) patients. Enzyme linked immunosorbent assay (ELISA) was applied to detect p40 antibody in the cerebrospinal fluid from those patients who were confirmed as BDV p24 positive in previous study and those patients who were detected as BDV positive by PCR in the present study.The confirmed p40 positive samples were used for BDV gene cloning and sequencing, which were subsequently compared with the sequences of overseas standard strains, including He/80, H1766 and strain V by software MEGA and DnaSP4.0. The virus phylogenetic tree was constructed based on the above data. Results The positive rate of BDV p24 by PCR was 10. 08% (12/119) and the positive rate of BDV p40 by PCR was 5.88% (7/119). The cerebrospinal samples from two patients were detected as nucleoprotein antibody positive by ELISA, which resulted in a positive rate of 1.68%. The results of gene sequence analysis showed that the sequences of 6 cases among the 7 BDV positive patients were exactly same as the sequence of German horse-origin He/80 strain. Only one synonymous mutation was detected in the remaining one case. Reconstructed gene phylogenetic tree showed a hybrid China (Ningxia)- German and Japan branch was formed by the BDV stain isolated from VE patients in Ningxia and the overseas BDV standard stains. Conclusions BDV infection may exist in VE patients in Ningxia Hui Autonomous Region, which may be associated with close contact between human and animals. The gene sequence of stains isolated from patients in Ningxia is highly homological with the overseas standard strain, which suggests that the domestic BDV may come from overseas, while domestic strains with mutations couldn't be excluded.

9.
Article in Chinese | WPRIM | ID: wpr-383230

ABSTRACT

Objective To investigate the expression and location of Borna disease virus(BDV)nucleoprotein in transfected oligodendrocytes,and then to explore its expression mechanisms in oligodendroglial cell(OL)and their effects on cell proliferation.Methods We used PCR to detect BDVp40 gene fragments in transfected OL,laser confocal microscopy and Western blot method to detect the expression of nuclear protein and its intracellular location in the cell,MTT to detect the influence of cell proliferation by nuclear protein on the OL.Results We had detected the BDVp40 gene fragments in transfected oligodendrocytes.The nucleoprotein can be positioned in the cytoplasm and cell membrane by laser scanning confocal microscope;Western blot results showed that there is nucleoprotein in the constitutive protein,but was not detected in the cytoplasm.MTT tests showed that nucleoprotein expressed in OL can inhibit cell proliferation.Conclusion It is indicated that the transfected OL stablely express of BDV nucleoprotein,and itslocation is in the cell structure of proteins,particularly in the cytoplasm and more richer in cell membrane,that indicating they may play a key role in cell signal transduction or into the cell-mediated viral infection.BDV nucleoprotein inhibit proliferation of OL,which maybe an important mechanism of Borna disease virus persistant infection and produce symptoms.

10.
Article in Chinese | WPRIM | ID: wpr-392734

ABSTRACT

Objective To study the effect of ShenQi FuZheng injection on side effects of chemotherapy and the patients' life quality after operation of colon cancer. Methods 60 patients were recruited into a control group and a treatment group, with 30 patients in each group. The treatment group was treated with conventional chemo-medicines plus ShenQi FuZheng injection, while the control group was treated with conventional chemo-medicines exclusively. Side effects and life quality were compared between the two groups after two courses of treatment. Results The side effects and mark of the life quality in the treatment group were both obviously better than the control group (P<0.05) after the treatment. Condusion ShenQi FuZheng injection can decrease side effects of chemotherapy and improve patients' life quality.

11.
Clinical Medicine of China ; (12): 876-878, 2008.
Article in Chinese | WPRIM | ID: wpr-399209

ABSTRACT

Objective To compare the effect of artianterior sacralia foramina block and sacral block on induced abortion.Mathods 263 women for induced abortion were randomly devided into three groups:the group for anteriora sacralia foramina block (n=95),the group for sacral block (n=90),the control group (n=78).Parameters before and after abortion measured include anesthesia area,skin of both legs and anus tempreture,blood pressure,pulse rate,resperatory rate,heart rate,clinical effect and VAS score.Results At 15 minutes after the anteriora sacralia foramina blocking,the anesthesia area involved distribution of medii clunium nerve,glacens nerve,puden-dus nerve,posterior femoris cutaneus nerve,ischiadicus nerve.The skin temperatures of both legs increased significantly (P<0.01).Blood pressure,pulse rate,resperatory rate,and heart rate didn't vary markedly.The analgisia effect of anteriora sacralia foramina block was superior to sacral block.Conclusion The anteriora sacralia foramina block is simple,safe,effective,practical and can become a new approach for analgisia in induced abortion.

12.
Article in Chinese | WPRIM | ID: wpr-578146

ABSTRACT

Objective:To investigate the changes of neuron-specific enolase(NSE)concentration in serum and cerebrospinal fluid from patients with acute and recovery viral encephalitis and to find out its correlation with anomaly electroencephalogram and low density focus volume in imageology. Methods:42 cases of patients with viral encephalitis were enrolled and 25 cases of health examinater or patients with operation of appendix or cholecyst were enrolled in control group.We measured the NSE concentration in cerebrospinal fluid and serum using enzyme-linked immunosorbent assay. Comparison analysis was performed by using a SPSS13.0 statistical package. Results:NSE concentration in serum and cerebrospinal fluid of patients with acute viral encephalitis was significantly higher than that of recoverys and control group(P

13.
Article in Chinese | WPRIM | ID: wpr-546872

ABSTRACT

[Objective]To investigate the effect of Zhenghai biological membrane in the prevention of adhesion of tendon. [Method]Eighteen chicken were divided into 2 groups.Zhenghai biological membrane was used in the experimental group,while simple Kessler suture was applied in the control group.The tendon adhesion degrees were compared between 2 groups with the methods of gross observation,adhesion criterion,histological observation and electron microscopy,et al.[Result]The adhesion degree was lower significantly in experimental group than that in control group.[Conclusion]Zhenghai biological membrane could prevent tendon adhesion effectively,without any obvious rejection reaction.It would not affect the healing of the tendon.

14.
Article in Chinese | WPRIM | ID: wpr-684480

ABSTRACT

Objective To discuss the differential diagnosis and treatment of bursitis around the knee. Methods 32 patients with bursitis around the knee were treated from March 2000 to March 2002 in our hospital. Their diagnosis and treatment were reviewed. The arthroscopy of knee confirmed all the initial diagnosis of the patients made by the physical examination and complaints. We gave them conservative treatment and analyzed the clinical follow ups. Results All the patients were followed up for 8 to 40 weeks. 9 of them who had no internal knee lesions but only one kind of bursitis healed within 3 weeks. 23 of them who had more than two kinds of bursitis (including four who had internal knee lesions) healed within 3 to 5 weeks. Conclusions The differential diagnosis of the bursitis around the knee can be made on the basis of complaints and physical examination. These cases can be managed by conservative treatment.

15.
Article in Chinese | WPRIM | ID: wpr-542577

ABSTRACT

Objective In order to investigate the relationship between Borna disease virus(BDV) and the viral encephalitis,and whether BDV infection exists in Ningxia or not.Methods The p24 fragment of BDV in peripheral blood mononuclear cells(PBMC) and cerebrospinal fluid mononuclear cells(CSFMC) in 52 patients with viral encephalitis and 32 healthy donors were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and fluorescence quantitative(FQ) PCR to analysis gene sequence,homogenicity and amino acid sequence of positive products.Results The positive rate of BDV p24 in PBMC and CSFMC in viral encephalitis(9.6%,11.5%) was significantly higher than that in healthy donors(0,P

16.
Article in Chinese | WPRIM | ID: wpr-411348

ABSTRACT

This paper was to observe the revaccination effect of domestic yeast-derived recombinant hepatitis B vaccine (YRHBV) to children who were born to HBsAg and HBeAg negative mothers and had been immunized with full course of blood-derived hepatitis B vaccine 4 years ago at their birth. The treated group was revaccinated with 5μg YRHBV while the control group with 10μg blood-derived HB vaccine, their anti-HBs response was determined 1,12,24 months after revaccination. The results showed that a nice anti-HBs response was discovered in group A childeren, no matter how about the anti-HBs level before revaccination was. The kinetics of anti-HBs after YDHBV revaccination was similar to that of the blood-derived HB vaccine, no statistical difference was found. So, 5μg of domestic yeast-derived recombinant HB vaccine gave same immune effect as well as 10μg blood-derived HB vaccine.

17.
Article in Chinese | WPRIM | ID: wpr-570088

ABSTRACT

0 05) Conclusions Mifepristone with misoprostol for terminating early pnegnaney might be affect the expression of lymphocyte and cytokines and induce the disorder of decidual micro environment which might be the reason of medical abortion

18.
Article in Chinese | WPRIM | ID: wpr-572525

ABSTRACT

Objective:To evaluate the clinical value of determinations of C-reactive protien concentration in serum and cerebrospinal fluid from patients with acute and recovery cerebral infarction and to find out the correlation of it with neurologic deficits,infarct sige and duration.Methods:46 cases of patients with cerebral infarction were recruited as observe group and 25 cases of healthy examinees or patients undergoing operation of fracture,appendix or cholecyst ectomy were recruited as control group.We measured C-reactive protien in cerebrospinal fluid and serum.Comparison analysis was performed using a SPSS10.0 statistical package.Results:Concentration of C-reactive protien in cerebrospinal fluid and serum was significantly higher in patients with cerebral infarction than that in patients at recovery stage and control group(P

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