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Article in Chinese | WPRIM | ID: wpr-283781


<p><b>OBJECTIVE</b>To extract and analysis the active components of Se-protein polysaccharide from Se-rich Cordyceps militaris, and to discuss the anti-tumor effect of Se-protein polysaccharide.</p><p><b>METHOD</b>Protein, polysaccharides and selenium content were determined by the methods of Folin-phenol reagent (lowry), phenol-sulfate and DAN fluorescence, respectively. Tumor-bearing mouse model was established and divided into the model group, cyclophosphamide group, cordyceps high and low dosage group (200, 100 mg x kg(-1)). Then the Se-protein polysaccharide's anti-tumor activity and immune function in vivo were observed by compare with model group in the weight of mice, inhibitory rate, conversion rate of peripheral blood lymphocytes, dissection index K, swallowed factor alpha, liver and spleen factor coefficient, GSH-Px and SOD activity and the content of MDA.</p><p><b>RESULT</b>Se-protein polysaccharides from Se-rich Cordyceps militaris had a significant anti-tumor action with the inhibitory rate 46.92% and could avoid toxic effect of chemotherapy drug like cyclophosphamide. It also could enhance immune function and body antioxidant capacity by inhibiting the decline of tumor-bearing mouse liver coefficient and spleen coefficient in tumor-bearing mice caused by cyclophosphamide.</p><p><b>CONCLUSION</b>Se-protein polysaccharide, the extraction of Se-rich Cordyceps militaris's can inhibit tumor grouth of tumor-bearing mouse.</p>

Animals , Antineoplastic Agents , Chemistry , Pharmacology , Antioxidants , Metabolism , Body Weight , Cordyceps , Chemistry , Enzyme Activation , Female , Fungal Proteins , Chemistry , Liver , Metabolism , Lymphocytes , Allergy and Immunology , Male , Mice , Polysaccharides , Chemistry , Pharmacology , Selenium , Chemistry , Spleen , Metabolism , Superoxide Dismutase , Metabolism
Chinese Journal of Biotechnology ; (12): 375-379, 2005.
Article in Chinese | WPRIM | ID: wpr-305266


Using SDS-PAGE analysis, the High-molecular-weight (HMW) glutenin subunits of two Crithopsis delileana accessions were detected. It was found that the two accessions had the same HMW glutenin subunits. Only one HMW glutenin subunit with the similar electrophoresis mobility to the y-type HMW glutenin subunit of hexaploid wheat was observed in C. delileana. However, It was indicated that this glutenin subunit was an x-type glutenin subunit Kx by gene sequence analysis. The full coding region of Kx gene is 2052bp and could encodes a mature protein with 661 amino acid residues. The Kx gene could be expressed in the bacterial expression system, and the expressed protein had the same electrophoresis mobility as that in the seed of C. delileana. The primary structure of Kx subunit was very similar to the x-type HMW glutenin subunits encoded by the A, B and D genomes of wheat, the C and U genomes of Aegilops, and the R genome of Secale cereale. In the phylogenetic analysis, Kx subunit was clustered together with Ax1 subunit by an interior parallelled branch. In conclsion, Kx gene is a novel x type glutenin subunit gene from C. delileana.

Amino Acid Sequence , Base Sequence , Genes, Plant , Glutens , Genetics , Metabolism , Molecular Sequence Data , Plant Proteins , Genetics , Metabolism , Poaceae , Genetics , Metabolism , Triticum , Genetics , Metabolism
Article in Chinese | WPRIM | ID: wpr-680388


Objective:To investigate the effect of carvedilol on expression of cardiac matrix metalloproteinases(MMPs)and tissue inhibitors of metalloproteinases(TIMPs)after myocardial infarction in rats.Methods:An animal model of acute myocar- dial infarction(AMI)was established by descending left coronary artery ligation in 24 rats and they were divided into carvedilol (10 mg?kg~(-1)?d~(-1))group(n=12)and normal saline group(n=12).Sham operated group(n=9)received the same proce- dure but with no ligation.All animals were treated for 6 weeks via a gastric lavage.Heart function and hemodynamic parame- ters were determined after 6 weeks.The protein expression of cardiac MMP-2,MMP-9 and TIMP-2 was detected by immuno- histoehemical analysis in AMI groups,and the MMPs activities were assessed by zymography.Gene expression of myocardial MMPs/TIMPs(MMP-2,9 and TIMP-1,2)and cytokines(TNF-?,IL-1?)were measured by real-time quantitative PCR.Re- suits:Compared with Sham-operated group,earvedilol group had significantly higher left ventricular end-diastolic pressure(LV- EDP)and lower LV upstroke velocity(+dp/dt_(max))and LV descent velocity(-dp/dt_(max))(P