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1.
Asian Journal of Andrology ; (6): 396-399, 2021.
Article in English | WPRIM | ID: wpr-888439

ABSTRACT

Optimal vision and ergonomics are essential factors contributing to the achievement of good results during microsurgery. The three-dimensional (3D) digital image microscope system with a better 3D depth of field can release strain on the surgeon's neck and back, which can improve outcomes in microsurgery. We report a randomized prospective study of vasoepididymostomy and vasovasostomy using a 3D digital image microscope system (3D-DIM) in rats. A total of 16 adult male rats were randomly divided into two groups of 8 each: the standard operating microscope (SOM) group and the 3D-DIM group. The outcomes measured included the operative time, real-time postoperative mechanical patency, and anastomosis leakage. Furthermore, a user-friendly microscope score was designed to evaluate the ergonomic design and equipment characteristics of the microscope. There were no differences in operative time between the two groups. The real-time postoperative mechanical patency rates were 100.0% for both groups. The percentage of vasoepididymostomy anastomosis leakage was 16.7% in the SOM group and 25.0% in the 3D-DIM group; however, no vasovasostomy anastomosis leakage was found in either group. In terms of the ergonomic design, the 3D-DIM group obtained better scores based on the surgeon's feelings; in terms of the equipment characteristics, the 3D-DIM group had lower scores for clarity and higher scores for flexibility and adaptivity. Based on our randomized prospective study in a rat model, we believe that the 3D-DIM can improve surgeon comfort without compromising outcomes in male infertility reconstructive microsurgery, so the 3D-DIM might be widely used in the future.

2.
Chinese Circulation Journal ; (12): 1071-1074, 2017.
Article in Chinese | WPRIM | ID: wpr-667941

ABSTRACT

Objective: To explore the relationship between the incidences of percutaneous coronary intervention (PCI) complicated depression and serum levels of brain-derived neurotrophic factor (BDNF), ghrelin in coronary artery disease (CAD) patients. Methods: A total of 90 CAD patients after PCI were enrolled. According to Hamilton depression (HAMD) scale, the patients were divided into 2 groups: Depression group, n=40 and Non-depression group, n=50. Serum levels of BDNF and Ghrelin were examined by ELISA and compared between 2 groups. Results: Compared with Non-depression group, Depression group had reduced serum levels of BDNF and Ghrelin, both P<0.05. As increased severity of depression, BDNF and Ghrelin were decreased accordingly; serum levels of BDNF and Ghrelin were negatively related to HAMD score (r=-0.711, P<0.05 and r=-0.711, P<0.05). Conclusion: Serum levels of BDNF and Ghrelin have an early warning effect on depression in CAD patients after PCI, it may reflect the severity of depression at certain degree in relevant patients.

3.
National Journal of Andrology ; (12): 536-538, 2014.
Article in Chinese | WPRIM | ID: wpr-309676

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the clinical effectiveness of transurethral seminal vesiculoscopy (TUSV) combined with finasteride in the treatment of recurrent hemospermia.</p><p><b>METHODS</b>This study included 32 patients with recurrent hematospermia, with the disease course of 3 months to 4 years. After administration of finasteride at 5 mg/d for 2 weeks, the patients underwent TUSV for both exploration of the causes and treatment, followed by medication with finasteride at the same dose for another 2 weeks. Postoperative follow-up was conducted for observation of the outcomes and complications.</p><p><b>RESULTS</b>TUSV was successfully accomplished in all the 32 cases, which revealed 16 cases of seminal vesiculitis, 10 seminal calculi, 1 seminal vesicle cyst, 2 seminal vesicle polyps, and 3 seminal vesicle abscess. The operative time was 20 to 51 (31.0 +/- 5.2) minutes. Postoperative complications included 1 case of acute epididymitis and 3 cases of breast discomfort within the first 4 weeks. No incontinence, urethral stricture, rectal injury, retrograde ejaculation, and sexual dysfunction occurred postoperatively. All the patients but 1 were followed up for 6 months to 2 years. Twenty-nine of the cases were cured, and 2 experienced recurrence.</p><p><b>CONCLUSION</b>Transurethral seminal vesiculoscopy combined with finasteride is safe and effective for the treatment of recurrent hemospermia.</p>


Subject(s)
Adult , Endoscopy , Methods , Finasteride , Therapeutic Uses , Follow-Up Studies , Hemospermia , Therapeutics , Humans , Male , Middle Aged , Retrospective Studies , Treatment Outcome
4.
Chinese Medical Journal ; (24): 476-481, 2013.
Article in English | WPRIM | ID: wpr-342559

ABSTRACT

<p><b>BACKGROUND</b>The purpose of this study was to investigate the feasibility of avoiding axillary lymph node dissection (ALND) for patients with only one sentinel lymph node (SLN) metastasis. The characteristics and predictive factors for non-sentinel lymph node (NSLN) metastasis of patients with single positive SLN were also analyzed.</p><p><b>METHODS</b>Patients with no and only one SLN metastasis (0/n and 1/n group, n ≥ 2) were selected from 1228 cases of invasive breast carcinoma, who underwent axillary dissection in Shandong Cancer Hospital between November 1999 and December 2011, to compare the characteristics of NSLN metastasis between them. For the 1/n group, the factors that influenced the NSLN metastasis were analyzed by univariate and multivariate analysis.</p><p><b>RESULTS</b>Differences of the NSLN metastasis between the 0/n and the 1/n groups were significant (P < 0.001). There was no significant difference between the axillary lymph node metastasis on level III in 1/n group and 0/n group (P = 0.570). When the total SLN number was ≥ 4 and with one positive case, the NSLN metastasis was not significantly different from that in the 0/n group (P = 0.118). In the 1/n group, clinical tumor size (P = 0.012), over-expression of Her-2 (P = 0.003), tumor grade (P = 0.018) and the total number of SLN (P = 0.047) significantly correlated with non-SLN metastasis. Clinical tumor size (P = 0.015) and the expression of Her-2 (P = 0.01) were independent predictive factors for non-SLN metastasis by the Logistic regression model.</p><p><b>CONCLUSION</b>Under certain conditions, breast cancer patients with single SLN metastasis could avoid ALND.</p>


Subject(s)
Adult , Aged , Breast Neoplasms , Pathology , Female , Humans , Lymph Nodes , Pathology , Lymphatic Metastasis , Pathology , Middle Aged , Sentinel Lymph Node Biopsy
5.
Chinese Journal of Oncology ; (12): 389-393, 2013.
Article in Chinese | WPRIM | ID: wpr-267533

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of this study was to investigate the clinicopathologic factors associated with false-negative rate of sentinel lymph node biopsy (SLNB) in breast cancer, and to explore how to reduce the false-negative rate of SLNB.</p><p><b>METHODS</b>The clinicopathological data of 2265 patients with invasive breast carcinoma who underwent sentinel lymph nodes biopsy (SLNB) in Shandong Cancer Hospital between November 1999 and December 2011 were retrospectively analyzed. We screened 1228 patients who received axillary lymph node dissection after SLNB, and studied the clinicopathological factors that could be associated with false-negative rate of SLNB.</p><p><b>RESULTS</b>The false negative rate of this group was 10.7% (73/683), accuracy rate was 94.1% (1155/1228), and negative predictive value was 88.2% (545/618). Clinical tumor size (all P < 0.05), calendar year of surgery (all P < 0.05) and numbers of detected SLNs (all P < 0.05) were significantly related with false negative rate and accuracy rate of SLNB, determined by single factor analysis. Logistic regression model analysis showed that calendar year of surgery (P = 0.034) and numbers of detected SLNs (P = 0.012) were independent predictive factors for the false negative rate of SLNB.</p><p><b>CONCLUSIONS</b>False negative rate and accuracy rate of SLNB are significantly related to the calendar year of surgery and number of detected SLNs. Strict case selection, standard operation procedure, increaseing numbers of detected SLNs, and improvement of the skill of operators are effective measures to reduce the false negative rate of SLNB.</p>


Subject(s)
Adult , Aged , Axilla , Breast Neoplasms , Pathology , General Surgery , Carcinoma, Ductal, Breast , Pathology , General Surgery , Carcinoma, Lobular , Pathology , General Surgery , Carcinoma, Medullary , Pathology , General Surgery , False Negative Reactions , Female , Humans , Lymph Node Excision , Lymph Nodes , Pathology , General Surgery , Lymphatic Metastasis , Middle Aged , Retrospective Studies , Sentinel Lymph Node Biopsy , Young Adult
6.
Chinese Medical Journal ; (24): 973-977, 2011.
Article in English | WPRIM | ID: wpr-239910

ABSTRACT

<p><b>BACKGROUND</b>Sentinel lymph node (SLN) biopsy has become a common procedure for early breast cancer patients. The GeneSearch(TM) Breast Lymph Node (BLN) Assay is a real-time RT-PCR assay for the detecting nodal metastases larger than 0.2 mm. China Breast Cancer Clinical Study Group (CBCSG)-001a is a prospective multi-center clinical trial that was conducted to validate the GeneSearch(TM) BLN Assay in China.</p><p><b>METHODS</b>The SLNs from 90 consecutive patients were identified and dissected, and then sectioned along the short axis into multiple blocks. Intra-operatively, the odd blocks were tested by BLN assay and the even ones were used for frozen section, while all the blocks were evaluated by touch imprint cytology. Post-operatively, the remaining tissues were assessed by histological evaluation.</p><p><b>RESULTS</b>A total of 189 SLNs was tested by BLN assay. The sensitivity, specificity, positive predictive value, and negative predictive value were 88.9%, 97.4%, 88.9% and 97.4%, respectively, for BLN assay, 75.0%, 100%, 100% and 94.4%, respectively, for frozen section, and 63.9%, 100%, 100% and 92.2%, respectively, for touch imprint cytology. The sensitivity of BLN assay was higher than that of touch imprint cytology (P = 0.01) and frozen section (P = 0.13). When assessing the nodes with micro-metastases, BLN assay had a significant higher sensitivity than frozen section (P = 0.023) and touch imprint cytology (P = 0.005).</p><p><b>CONCLUSION</b>The GeneSearch(TM) BLN Assay is an accurate and rapid intra-operative assay for breast SLNs and it is suitable for application in general medical practice.</p>


Subject(s)
Adult , Aged , Breast Neoplasms , Female , Humans , Lymph Nodes , Pathology , Lymphatic Metastasis , Diagnosis , Middle Aged , Sentinel Lymph Node Biopsy , Methods
7.
National Journal of Andrology ; (12): 150-153, 2010.
Article in Chinese | WPRIM | ID: wpr-252804

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the restoration of erectile function by reconstructing cavernous nerves (CN) with small intestinal submucosa (SIS) grafts.</p><p><b>METHODS</b>We prepared SIS grafts, established rat models and divided the models into a CN ablation, a sham-operation and an SIS graft group. The CNs at both sides were severed with 1 cm ablated in the first group, and 0.5 cm removed in the third, followed by reconstruction with the SIS grafts. Three months after surgery, the apomorphine test was performed to evaluate the erectile function, and then all the rats were sacrificed to detect the expression of nNOS in the penis.</p><p><b>RESULTS</b>Penile erection was observed in 72.73% (8/11) of the rats for (1.07 +/- 0.89) times within 30 min in the SIS graft group, as compared with 0% (0/11) of the rats for (0.00 +/- 0.00) times in the CN ablation group (P < 0.01), and 90.91% (10/11) of the rats for (2.19 +/- 1.17) times in the sham-operation group (P < 0.01). The number of nNOS nerve fibers was significantly larger in the SIS graft than in the CN ablation group (70.36 +/- 10.09 versus 22.09 +/- 4.76, P < 0.01), but both were significantly smaller than that of the sham-operation group (90.81 +/- 5.69, P < 0.01).</p><p><b>CONCLUSION</b>The SIS grafting technique contributes to the recanalization of the severed CN and restoration of erectile function in rats after surgical injury.</p>


Subject(s)
Animals , Erectile Dysfunction , General Surgery , Intestinal Mucosa , Transplantation , Intestine, Small , Male , Nerve Regeneration , Nerve Tissue , Wounds and Injuries , General Surgery , Penile Erection , Penis , Rats , Rats, Sprague-Dawley
8.
National Journal of Andrology ; (12): 834-839, 2010.
Article in Chinese | WPRIM | ID: wpr-294989

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the restoration of rat penile erection by reconstructing injured cavernous nerves (CN) with a compound graft prepared from porcine small intestinal submucosa (SIS) and Schwann cells (SC).</p><p><b>METHODS</b>SCs were cultured in vitro and a compound graft was prepared from the SCs and SIS. Thirty-three healthy SD rats were randomly divided into three groups of equal number, sham-operation, CN ablation, and SIS + SC graft. Three months after the operation, all the rats underwent the apomorphine test, followed by immunohistochemical staining of the tissues from the middle part of the corpus cavernosum penis.</p><p><b>RESULTS</b>Combined use of mechanical stripping, mixed-enzyme digestion, different-speed adhesion, short-term Ara-C and some other methods yielded SCs of a purity high enough for nerve tissue engineering. The SIS prepared by mechanical and chemical methods exhibited a good biocompatibility with SCs, which could adhere, grow, propagate and differentiate on its surface. The apomorphine test showed that both the rate and frequency of penile erection were significantly higher in the SIS + SC graft than in the CN ablation group (P < 0.01), but lower than in the sham operation group (P < 0.01). The number of nNOS positive nerve fibers in the SIS + SC graft group was significantly different from that of the CN ablation (P < 0.01), but both were smaller than that of the sham-operation group.</p><p><b>CONCLUSION</b>The compound of SIS with SCs, as a nerve graft, can be used to reconstruct injured cavernous nerves, and to some extent, restore penile erectile function.</p>


Subject(s)
Animals , Intestinal Mucosa , Transplantation , Intestine, Small , Male , Nerve Regeneration , Penile Erection , Penis , General Surgery , Rats , Rats, Sprague-Dawley , Schwann Cells , Transplantation , Swine , Transplantation, Heterologous
9.
Article in Chinese | WPRIM | ID: wpr-307065

ABSTRACT

<p><b>OBJECTIVE</b>Ultrasound scanning can provide the information of acoustic impedance through the ultrasonic reflection theory. This study tries to introduce the application of the ultrasonic technique in predicting biomechanical properties of cancellous bone.</p><p><b>METHODS</b>The rat femoral head embedded in plexiglass was used as the bone specimens for ultrasound scanning and the gray scale images scanned by ultrasound microscope were collected and analyzed.</p><p><b>RESULTS</b>The value of acoustic impedance was calculated by analyzing the gray scale image.</p><p><b>CONCLUSION</b>The application of ultrasonic microscopy can show more value about the microstructure of biomechanical properties of bone tissue.</p>


Subject(s)
Animals , Biomechanical Phenomena , Bone Density , Femur Head , Diagnostic Imaging , Physiology , Microscopy, Acoustic , Methods , Osteoporosis , Rats
10.
National Journal of Andrology ; (12): 530-532, 2008.
Article in Chinese | WPRIM | ID: wpr-319199

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the seminal parameters, zinc concentration and antibacterial activity in patients with non- inflammatory chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS).</p><p><b>METHODS</b>Seminal parameters, zinc concentration and antibacterial activity of seminal plasma were detected in 60 CP/CPPS patients and 20 normal men.</p><p><b>RESULTS</b>Statistically significant differences were found in the duration of semen liquefaction, sperm vitality, sperm motility, zinc concentration and antibacterial activity of the seminal plasma between the CP/CPPS and the control males ( P < 0.01). Zinc concentration was significantly correlated with the duration of sperm motility (r = 0. 272, P = 0.015) and antibacterial activity of the seminal plasma (r = 0.449, P < 0.01) in the CP/CPPS patients.</p><p><b>CONCLUSION</b>CP/CPPS has a significant negative impact on semen liquefaction, sperm motility and vitality, zinc concentration and antibacterial activity of seminal plasma. The antibacterial activity of seminal plasma is positively correlated with zinc concentration and sperm motility.</p>


Subject(s)
Chronic Disease , Humans , Male , Microbial Sensitivity Tests , Pelvic Pain , Pathology , Prostatitis , Semen , Chemistry , Cell Biology , Sperm Count , Sperm Motility , Syndrome , Zinc
11.
Acta Pharmaceutica Sinica ; (12): 535-541, 2008.
Article in Chinese | WPRIM | ID: wpr-277817

ABSTRACT

The aim of this study is to develop a convenient and effective method for the identification of heparin from pigs (include Sus scrofa domestica Brisson and Sus scrofa riukiuanus). Based on sequences of D-loop region of pigs and the other animals, two pairs of highly specific primers were designed for distinguishing heparin of pigs from other animals. The primers were employed to amplify D-loop region of DNA templates extracted from pig and seven other animal species that amounted to 49 samples. AS-PCR (allele-specific PCR) and ARMS (amplification refractory mutation system) were all suitable for fast identification of heparin from pig with anneal temperature at 54-56 degrees C in AS-PCR and with wider anneal temperature in ARMS,at 52-58 degrees C. An about 170 bp DNA fragments were amplified from separately pigs and whereas no DNA fragment was amplified from other samples under the same reaction condition.


Subject(s)
Alleles , Animals , Base Sequence , DNA Mutational Analysis , Methods , DNA Primers , DNA, Mitochondrial , Genetics , Drug Contamination , Heparin , Genetics , Horses , Genetics , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Methods , Quality Control , Ruminants , Genetics , Sequence Alignment , Sequence Analysis, DNA , Sus scrofa , Genetics
12.
Article in Chinese | WPRIM | ID: wpr-305501

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the necessity of detecting on the expressive intensity and pattern of HBsAg and HBcAg in the livers of chronic hepatitis B.</p><p><b>METHODS</b>HBsAg and HBcAg were detected in paraffin-embedded liver tissue by EnVision immunohistochemistry. Serum hepatitis B virus DNA (HBV DNA) was tested by real-time quantitative polymerase chain reaction. The degrees of hepatic inflammatory activity (grade) and fibrosis (stage) of liver biopsies were determined according to the standard of the Chinese program of prevention and treatment of viral hepatitis.</p><p><b>RESULTS</b>The expression of HBsAg was not correlated with the grade, the stage and the levels of serum HBV DNA (P > 0.05). Liver HBcAg expressive intensity was not correlated with the grade (r=0.02, P > 0.05), while negatively correlated with the stage (r=0.28, P < 0.01) and positively correlated with the serum HBV DNA levels (r=0.53, P < 0.01). Liver HBcAg expressive pattern was negatively correlated with the grade (r=-0.27, P < 0.01). The grade in cytoplasmic pattern group was higher than in nuclear pattern group and in mixed pattern group (P < 0.01), and that in mixed pattern group was higher in nuclear pattern group (P < 0.01). Liver HBcAg expressive pattern was negatively correlated with the stage (r=-0.23, P < 0.01). The stage in cytoplasmic pattern group was higher than in nuclear pattern group and in mixed pattern group (P < 0.05). Liver HBcAg expressive pattern was positively correlated with the levels of serum HBV DNA (r=0.22, P < 0.01).</p><p><b>CONCLUSION</b>Distinguishing the expressive intensity and pattern of HBsAg and HBcAg in the liver of chronic hepatitis B may not help understand the degree of hepatic lesion. The detection of HBcAg in liver tissue of CHB may be beneficial for the antiviral therapy.</p>


Subject(s)
Adult , DNA, Viral , Blood , Genetics , Female , Hepatitis B Antigens , Hepatitis B Core Antigens , Hepatitis B Surface Antigens , Hepatitis B virus , Genetics , Allergy and Immunology , Physiology , Hepatitis B, Chronic , Pathology , Virology , Host-Pathogen Interactions , Humans , Immunohistochemistry , Liver , Pathology , Virology , Male , Middle Aged , Reverse Transcriptase Polymerase Chain Reaction , Virus Replication , Young Adult
13.
Chinese Journal of Oncology ; (12): 515-517, 2006.
Article in Chinese | WPRIM | ID: wpr-236945

ABSTRACT

<p><b>OBJECTIVE</b>The purpose of the present study was to explore the expression and clinical significance of multiple tumor suppressor gene 1 (MTS1) and cyclooxygenase-2 (COX-2) gene in invasive breast cancers.</p><p><b>METHODS</b>Flow cytometry was used to analyze the expression level of MTS1 and COX-2 in cancer tissues and corresponding para-cancer tissues from 66 cases of primary invasive breast cancers.</p><p><b>RESULTS</b>In breast cancer tissues, the expression of MTS1 and COX-2 assessed by relative fluorescence intensity were 0.84 and 10.54, respectively, and were 1.61 and 4.00 in corresponding para-cancer tissues, respectively. There was a significant difference between MTS1 and COX-2 expressions in cancer and corresponding para-cancer tissues (P <0.05). The differences of MTS1 and COX-2 expression of different ages, pathological types, tumor sizes or clinical stages of the breast cancer patients were not significant (P > 0.05). The MTS1 and COX-2 expressions were 1.12 and 5.94, respectively, in lymph node metastasis positive patients, and 0.79 and 13.05, respectively, in lymph node metastasis negative patients. The differences were significant (P <0.05).</p><p><b>CONCLUSION</b>The preliminary research results suggest that MTS1 and COX-2 gene expressions play fairly important role in tumorigenesis and progression of breast cancers. MTS1 and COX-2 protein expressions have correlation with lymph node metastasis. This study provides theoretical basis for use of COX-2 selective inhibitors in prevention and treatment for breast cancer patients.</p>


Subject(s)
Adult , Aged , Biomarkers, Tumor , Metabolism , Breast , Metabolism , Pathology , Breast Neoplasms , Metabolism , Pathology , Carcinoma, Ductal, Breast , Metabolism , Pathology , Cyclin-Dependent Kinase Inhibitor p16 , Metabolism , Cyclooxygenase 2 , Metabolism , Female , Flow Cytometry , Humans , Lymphatic Metastasis , Middle Aged , Neoplasm Staging
14.
Chinese Journal of Hematology ; (12): 522-524, 2006.
Article in Chinese | WPRIM | ID: wpr-243920

ABSTRACT

<p><b>OBJECTIVE</b>To analyse the outcome of allogeneic peripheral blood stem cell transplantation (allo-PBSCT) for myelodysplastic syndromes (MDS).</p><p><b>METHODS</b>Twenty-three patients with MDS received G-CSF mobilized HLA-identical sibling allo-PBSCT. The numbers of mononuclear cells (MNC) and CD34+ cells were 8. 25 (4.50 -22.36) x 10(8)/kg and 5.59 (1.57 - 12.22) x 10(6)/kg respectively. CsA and shorten course MTX were used for graft-versus-host disease (GVHD) prophylaxis and MMF was given on + 1 d - +28 d posttransplantation.</p><p><b>RESULTS</b>Among 23 patients, 22 achieved hematopoietic recovery. The median time of ANC > 1.0 x 10(9)/L and BPC > 50 x 10(9)/L were + 13 (+ 11 - +17) days and + 30 (+13 + 102) days respectively. Two patients died of transplant related complications and three died of disease relapse, while 18 patients survived. Kaplan-Meier analysis showed disease free survival and relapse rate were (77.8 +/- 8.7)% and (14.4 +/- 7.5)% respectively.</p><p><b>CONCLUSION</b>Allo-PBSCT is an effective treatment for MDS patients.</p>


Subject(s)
Adolescent , Adult , Antigens, CD34 , Erythrocyte Transfusion , Female , Graft Survival , Graft vs Host Disease , Humans , Male , Middle Aged , Myelodysplastic Syndromes , General Surgery , Peripheral Blood Stem Cell Transplantation , Methods , Transplantation, Homologous , Treatment Outcome
15.
Article in Chinese | WPRIM | ID: wpr-356734

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the triterpenoids from root of Achyranthes bidentata in Henan.</p><p><b>METHOD</b>Sephadex, normal-and reversed-phase column chromatographies were applied for the isolation and purification. The structure determinations were performed by means of physiochemical properties, MS and NMR data analyses.</p><p><b>RESULT</b>Seven compounds were isolated from the water soluble fraction in root of A. bidentata, and determined as achyranthoside A (1), achyranthoside E (2), momordin Ib (3), chikusetsusaponin IVa (4), chikusetsusaponin IVa methyl ester (5), chikusetsusaponin V (6), chikusetsusaponin V methyl ester (7).</p><p><b>CONCLUSION</b>Compounds 1 and 2 were isolated from the natural resources for the first time.</p>


Subject(s)
Achyranthes , Chemistry , Molecular Conformation , Molecular Structure , Oleanolic Acid , Chemistry , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Saponins , Chemistry
16.
Chinese Journal of Oncology ; (12): 420-422, 2005.
Article in Chinese | WPRIM | ID: wpr-358615

ABSTRACT

<p><b>OBJECTIVE</b>To study the relation between histopathologic grading and some of the cytogenetic and molecular biology characteristics of breast cancer.</p><p><b>METHODS</b>On the basis of estrogen receptor (ER) expression, DNA content, S-phase fraction (SPF), bcl-2 and mutant p53 protein (mtp53) expression were examined by FCM in 121 breast cancer patients. In 66 patients with invasive ductal breast cancer, histopathologic grading was also examined.</p><p><b>RESULTS</b>The aneuploidy rate and DNA index (DI) were significantly different in grade I, II and III breast cancer. SPF and mtp53 expression significantly increased with increase in histopathologic grading (P < 0.05), but bcl-2 did not show this trend. SPF and mtp53 expression were significantly more in breast cancer with negative ER than in those with positive ER (P < 0.05). Again, no such differences in bcl-2 regardless of ER expression. Correlations existed between DI vs SPF, DI vs mtp53, and SPF vs mtp53 expressions (P < 0.01) but bcl-2 did not correlate with any one of them.</p><p><b>CONCLUSION</b>Cytogenetic and molecular biology studies on the basis of histopathologic grading may provide more information in prognostic prediction of breast cancer.</p>


Subject(s)
Adolescent , Adult , Aneuploidy , Breast Neoplasms , Metabolism , Pathology , Carcinoma, Ductal, Breast , Metabolism , Pathology , DNA, Neoplasm , Female , Flow Cytometry , Humans , Middle Aged , Mutation , Prognosis , Proto-Oncogene Proteins c-bcl-2 , Genetics , Receptors, Estrogen , Genetics , S Phase , Tumor Suppressor Protein p53 , Genetics
17.
Article in Chinese | WPRIM | ID: wpr-356547

ABSTRACT

Immunotherapy of tumor is extensively attentioned as an important part of combined therapy of tumor in recent years. Dendritic cell (DC) is the most powerful antigen presenting cell (APC) by now which not only activates auto-immunity to attack tumor cells, but also does help to enhance antitumor effect for allogenic bodies. To explore the feasibility and safety of clinical therapy application of peripheral blood derived DC cultured ex vivo, and analyze the influence of DC-inducing-immunotherapy upon long-term survival of ANLL patients accepted autologous bone marrow transplantation, peripheral blood mononuclear cells (PBMNC) of 13 ANLL patients after autologous bone marrow transplantation were collected by using CS3000Plus. DC immunotherapy was administered after cultivation of PBMNC ex vivo for 2 weeks, desease-free survival time was observed after therapy for long time follow-up. The results showed that no any severe adverse event associated with DC therapy was observed, the survival analysis of Kaplan-Meier suggested that five year survival rate was 75.52% in DC group while 45.71% in non-DC group. DC group surpassed non-DC group in accumulative survival rate. It is concluded that the ex vivo cultivation and clinical therapy application of DC derived from peripheral blood are feasible and safe, DC immunotherapy in patients with acute non-lymphocytic leukemia after autologous bone marrow transplantation prolongs desease-free survival time and enhances long-term survival rate.


Subject(s)
Adolescent , Adult , Bone Marrow Transplantation , Cells, Cultured , Combined Modality Therapy , Dendritic Cells , Cell Biology , Allergy and Immunology , Transplantation , Female , Flow Cytometry , Humans , Immunotherapy, Adoptive , Kaplan-Meier Estimate , Leukemia, Monocytic, Acute , Allergy and Immunology , Pathology , Therapeutics , Leukemia, Myeloid, Acute , Allergy and Immunology , Pathology , Therapeutics , Leukemia, Myelomonocytic, Acute , Allergy and Immunology , Pathology , Therapeutics , Male , Middle Aged
18.
Article in Chinese | WPRIM | ID: wpr-355226

ABSTRACT

<p><b>OBJECTIVE</b>To establish a mouse model of heterotopic heart transplantation.</p><p><b>METHODS</b>In isotransplantation,BALB/c mice were used as both donors and recipients. In allotransplantation, C57 mice were used as donors and BALB/c mice as recipients. The hearts of donor mice were transplanted into the abdominal cavity of recipient mice, connecting aortic ascent artery of donor mice and abdominal aortic artery of recipient mice, main pulmonary artery of donor mice and inferior vena cava of recipient mice.</p><p><b>RESULTS</b>The mouse model of heterotopic heart transplantation was established successfully with a success rate of 90 %. The mean time of hot ischemia and cold ischemia were (0.9 +/-0.05) min and (34.8 +/-0.7) min, respectively. The survival time of isograft was more than 100 days and that of allograft was (7.7 +/- 0.3) days.</p><p><b>CONCLUSION</b>The operational procedure of donor heart and the quality of blood vessel anastomosis are two key points for successful heterotopic heart transplantation.</p>


Subject(s)
Animals , Heart Transplantation , Male , Mice , Mice, Inbred BALB C , Mice, Inbred C57BL , Models, Animal , Peritoneal Cavity , Transplantation, Heterotopic
19.
Article in Chinese | WPRIM | ID: wpr-352055

ABSTRACT

Dendritic cell (DC), the most powerful antigen presenting cell, has been paid close attention to tumor immunotherapy. The tumor antigens were extracted by different ways to pulse DC, and then the mature DC were infused to patients to accelerate anti-tumor immuno responses. Its clinical use would provide a new therapeutic pathway other than surgical operation, irradiation, chemotherapy. In this paper, biological characteristics of DC, tumor-vaccine preparation and its clinical use were reviewed.


Subject(s)
Cancer Vaccines , Allergy and Immunology , Cell Culture Techniques , Dendritic Cells , Allergy and Immunology , Humans , Neoplasms , Therapeutics
20.
Chinese Journal of Oncology ; (12): 183-185, 2003.
Article in Chinese | WPRIM | ID: wpr-347464

ABSTRACT

<p><b>OBJECTIVE</b>To determine the clinical results of selected CD34(+) cell autologous transplantation in advanced malignant tumors.</p><p><b>METHODS</b>After pretreatment, fifteen patients aged 12 - 70 (49.5) years with various Stage III or IV malignant tumors were given the sorted CD34(+) cells collected by magnetic-activated cell sorting (Clini MACS, Milteny Biotech, Germany).</p><p><b>RESULTS</b>Peripheral blood progenitor cells (PBPC) from the patients were mobilized by chemotherapy and G-CSF 5 micro g/kg per day. CD34(+) cells gave 2.0 - 5 log depletion after cell sorting, with a median yield of CD34(+) selected cells of 2.4 (0.15 - 12.03) x 10(6)/kg. It gave a median recovery of 64 (52 - 81.4)% and median purity of 98.2 (83.2 - 99.7)%. The median time of neutrophil recovery > 1.0 x 10(9)/L and platelet recovery > 20 x 10(9)/L post-transplantation were 14 (8 - 26) days and 13 (11 - 35) days, respectively. On follow-up of 2 - 33 (11) months, the event-free survival rate was 53.3% (8/15) and the overall survival rate was 66.7% (10/15).</p><p><b>CONCLUSION</b>Transplantation of autologous selected PBPC CD34(+) cells gives prompt and stable engraftment. Selected CD34(+) cell transplantation, being a safe approach, may improve the clinical outcome even in patients with advanced malignant tumors.</p>


Subject(s)
Adolescent , Adult , Aged , Antigens, CD34 , Child , Female , Hematopoietic Stem Cell Transplantation , Humans , Male , Middle Aged , Neoplasms , Mortality , Therapeutics , Survival Rate , Transplantation, Autologous
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