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1.
Article in English | WPRIM | ID: wpr-927652

ABSTRACT

Objectives@#It is unclear whether G protein-coupled receptor 61 (GPR61) affecting body weight, plays a role in the association between birth weight and weather. This study aimed to assess the effects of prenatal weather and GPR61 on birth weight.@*Methods@#A total of 567 mother-newborn pairs were recruited in Houzhai Center Hospital during 2011-2012. We detected the maternal and neonatal GPR61 promoter methylation levels, and obtained meteorological and air pollution data.@*Results@#A positive association was observed between maternal and neonatal GPR61 methylation levels, and both of them were affected by precipitation, relative humidity (RH) and daily temperature range (DTR). Birth weight was associated negatively with RH and positively with DTR ( P < 0.05). A significant association was observed between birth weight and neonatal GPR61 methylation. We observed that maternal GPR61 methylation seemed to modify associations between weather and birth weight ( P interaction < 0.10), while neonatal GPR61 methylation mediated the effects of RH and DTR on birth weight ( P < 0.05).@*Conclusions@#Our findings revealed the significant associations among prenatal weather, GPR61 methylation and birth weight. Maternal GPR61 methylation may modify the susceptibility of birth weight to prenatal weather conditions, while neonatal GPR61 methylation may be a bridge of the effects of prenatal RH and DTR on birth weight.


Subject(s)
Air Pollution/analysis , Birth Weight , Female , Humans , Infant, Newborn , Nerve Tissue Proteins , Pregnancy , Receptors, G-Protein-Coupled/metabolism , Temperature , Weather
2.
Article in Chinese | WPRIM | ID: wpr-879014

ABSTRACT

To establish the method for determining non-volatile ingredients of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid A, rosmarinic acid, ferulic acid, rutin, luteoloside, isoquercitrin, hesperidin, diosmin, diosmetin, luteolin, acacetin and linarin in Menthae Haplocalycis Herba formula granules and traditional herbal pieces by UPLC-MS/MS, and analyze the correlation of non-volatile ingredients in Menthae Haplocalycis Herba formula granules and traditional herbal pieces. Shim-pack GIST C_(18) column(2.1 mm×100 mm, 2 μm) was adopted with acetonitrile-0.1% formic acid aqueous solution as the mobile phase for gradient elution at the flow rate of 0.4 mL·min~(-1). The column temperature was set at 35 ℃. The quantitative analysis was performed using the electrospray ionization source and the multiple reaction monitoring. The linear relationship, resolution, repeatability and recovery of the 16 chemical components all met the requirements. The 16 non-volatile ingredients in traditional herbal pieces of Menthae Haplocalycis Herba could be tracked in formula granules. There were certain differences of the 16 chemical components among Menthae Haplocalycis Herba formula granules of different manufacturers and traditional herbal pieces of different producing areas. The UPLC-MS/MS method was simple, rapid and accurate, and could be used for the quality control of non-volatile ingredients in Menthae Haplocalycis Herba formula granules and traditional herbal pieces.


Subject(s)
Chromatography, High Pressure Liquid , Chromatography, Liquid , Correlation of Data , Drugs, Chinese Herbal , Tandem Mass Spectrometry
3.
Article in Chinese | WPRIM | ID: wpr-876232

ABSTRACT

Objective To investigate the prevalence of diabetes and impaired glucose regulation in high risks community residents in Songjiang District of Shanghai, and to explore the risk factors affecting their blood glucose metabolism, providing effective suggestions for improving community diabetes prevention and treatment. Methods Questionnaire, physical examination and laboratory testing were used to collect information on the basic characteristics and blood glucose levels of 21 035 residents in Songjiang District who were assessed to be at high risk of diabetes. Results A total of 3 008 people with impaired regulation and 2 241 patients with diabetes were detected.The detection rates were 14.3% and 10.6%, respectively.It was found that as the residents′ age was higher, their education level was lower, high-risk factors for them were numerous, and their detection rates of diabetes and impaired sugar regulation were higher.It was found that age, gender, history of impaired glucose regulation, relatives with type 2 diabetes, hypertension, dyslipidemia and overweight/obesity were all factors influencing blood glucose metabolism (P < 0.05). Conclusion Blood glucose screening in high-risk population of diabetes is helpful for the early detection of diabetic patients and those with impaired glucose regulation.It is necessary to strengthen the attention of population at risk of diabetes and take appropriate interventions.

4.
Article in Chinese | WPRIM | ID: wpr-743446

ABSTRACT

Objective To explore the action mechanism of moxibustion in treating Crohn's disease (CD) by observing the effect of herbal cake-partitioned moxibustion on the expression of NLRP3 inflammasome (NLRP3, ASC and Caspase-1) and IL-1β. Method Male Sprague Dawley (SD) rats of clean conventional grade were randomized into a normal control group (NG), a model control group (MG), a herbal cake-partitioned moxibustion control group (MoxG), and a sham moxibustion control group (SMG). The CD rat models were developed by using the mixture of 2, 4, 6-trinitrobenzene sulfonic acid (TNBS) and alcohol via enema. When the models were successfully developed, the MoxG received moxibustion intervention at bilateral Tianshu (ST25) and Qihai (CV6), and the SMG were administered by unlighted moxa cones. At the end of treatment, the length of each rats' colon and the colon macroscopic damage index (CMDI) score were recorded, the histopathological variations of rats' colons were observed by adopting HE staining and light microscope, and the expressions of NLRP3, ASC, Caspase-1 and IL-1β in rats' colons were determined by using immunohistochemical technique. Result Compared to the NG, the MG had its rats' colons present with severe damages, fissured ulcers and inflammatory cell infiltration with edema, and granulomas in submucosa of some colons, and its expressions of NLRP3, ASC, Caspase-1 and IL-1β increased significantly (P<0.05);compared to the MG, the MoxG had rats' colons present with improved structures and reduced intestinal inflammation, and its expressions of NLRP3, ASC, Caspase-1 and IL-1β dropped significantly (P<0.05); the SMG had its rats' colon inflammation present similarly to the MG, and its expressions of NLRP3, ASC, Caspase-1 and IL-1β had no significant difference (P>0.05). Conclusion Herbal Cake-partitioned moxibustion can down-regulate the expressions of NLRP3, ASC, caspase-1 and IL-1β in CD rats' colons to promote the repair of colon damage.

5.
Article in Chinese | WPRIM | ID: wpr-779420

ABSTRACT

Objective To describe the results of screening for high-risk population of diabetes mellitus among community residents in Shanghai from 2016 to 2017 and analyze the characteristics of subjects with impaired glucose regulation or diabetes patients. Methods After the diabetes risk assessment for the residents aged 35 and older in Changning and Songjiang district of Shanghai, physical examination and blood glucose test were conducted for high-risk groups. Results A total number of 33 469 people in the two communities were screened, 4 555 patients with impaired glucose regulation and 3 412 patients with diabetes were detected. The detection rates were 13.6% and 10.2%, respectively. In high-risk population, aging was significantly correlated with the risk of diabetes(males:P<0.001;females:P<0.001) and impaired glucose regulation(males:P<0.001;females:P<0.001). The risk of diabetes in rural males was lower than that in urban males (OR=0.873,95% CI:0.771-0.988), while rural females had a higher risk of impaired glucose regulation than urban females (OR=1.249, 95% CI: 1.131-1.379). Self-reported history of impaired glucose regulation(males:P<0.001;females:P<0.001), family history of type 2 diabetes(males:P<0.001;females:P<0.001), hypertension(males:P<0.001;females:P<0.001), dyslipidemia(males:P<0.001;females:P=0.015), overweight/obesity(males:P<0.001;females:P<0.001), long-term sedentary lifestyle in males (P=0.002) and a history of polycystic ovary syndrome in females (P=0.011) were related to the risk of dysglycemia. The more of the number of high-risk factors for residents, the higher the risk of impaired glucose regulation and diabetes (P<0.001). Conclusions The situation of community diabetes detection and prevention in Shanghai is very serious. Therefore, it is necessary to strengthen the monitoring of high-risk groups of diabetes and take targeted intervention measures to reduce the occurrence of diabetes in high-risk population.

6.
Article in Chinese | WPRIM | ID: wpr-905104

ABSTRACT

Objective:To observe the efficacy of laminoplasty with reconstructing of cervical extensor attachment on cervical spondylotic myelopathy (CSM) involving C2 segment. Methods:From March, 2014 to January, 2017, 46 cases with CSM involving C2 accepted surgery in our hospital. They were divided into two groups according to the surgical methods. Control group (n = 21) accepted traditional laminoplasty, while observation group (n = 25) accepted laminoplasty with extensor muscle attachment point reconstruction. They were assessed with Japanese Orthopaedic Association (JOA) spinal scores, cervical range of motion (ROM), cervical curvature, areas of posterior cervical muscles and axial symptoms. Results:There was no significant difference at operative time and intraoperative blood loss (t < 0.863, P > 0.05) between groups. After surgery, the JOA score increased in both groups (F > 24.961, P < 0.001), but there was no significant difference between two groups (t < 0.282, P > 0.05). ROM varied little in both groups (F < 0.931, P > 0.05). The cervical neutral position curvature decreased in the control group (F = 8.241, P < 0.01), but not in the observation group (F = 2.705, P > 0.05). The areas of posterior muscle decreased in control group (t = 2.678, P < 0.05), but not in the observation group (t = 0.854, P > 0.05). The incidence of axial symptoms was less in the observation group than in the control group (Z = -2.192, P < 0.05). Conclusion:Laminoplasty could relieve the spinal compression at C2 segment and promote the recovery of neurological function, and it can do better in cervical curvature and posterior cervical muscle atrophy as combination with reconstruction of extensor muscle attachment, to reduce the axial symptoms.

7.
Article in Chinese | WPRIM | ID: wpr-712676

ABSTRACT

Objective:To observe the effect of moxibustion on the protein and mRNA expressions of corticotropin-releasing factor (CRF)and corticotropin-releasing factor receptor 1 (CRFR1) in hypothalamus of trinitrobenzene sulfonic acid (TNBS)-induced experimental colitis rats,and to explore the central mechanisms of moxibustion in improving visceral pain and the pain-related emotions in experimental colitis rats.Methods:Thirty-six Sprague-Dawley (SD) rats were randomly divided into a normal group (NG),a model group (MG),a herb-partitioned moxibustion group (HPMG) and a sham herb-partitioned moxibustion group (SHPMG).Except the NG,rats in the remaining three groups all received TNBS enema to establish experimental colitis models.The HPMG received herb-partitioned moxibustion (HPM) at bilateral Tianshu (ST 25) and Qihai (CV 6) for intervention;for the SHPMG,the herbal cakes and moxa cones were only placed on the acupoints but not ignited;rats in the MG and NG were only fixed in the same way as those in the HPMG but did not receive any treatment.At the end of the intervention,the abdominal withdrawal reflex (AWR) score,the open field test (OFT) score and the elevated plus maze (EPM) score were observed to measure the changes in visceral pain and pain-related emotions of the rats.The enzyme-linked immunosorbent assay (ELISA) was used to examine the expressions of CRF and CRFR1 proteins in hypothalamus;the fluorescence-based quantitative polymerase chain reaction (PCR) was used to detect the expressions of CRF and CRFR1 mRNAs in hypothalamus.Results:Compared with the NG,the AWR score increased significantly and the OFT and EPM scores dropped significantly in the MG (all P<0.05),and the expressions of hypothalamic CRF and CRFR1 proteins and mRNAs increased significantly (all P<0.01).Compared with the MG and SHPMG,the AWR score dropped significantly and the OFT and EPM scores increased significantly in the HPMG (all P<0.01),and the expressions of hypothalamic CRF and CRFR1 proteins and mRNAs decreased significantly (all P<0.05).There were no significant differences between the MG and the SHPMG (all P>0.05).Conclusion:HPM can down-regulate the abnormally increased expressions of CRF and CRFR1 proteins and mRNAs in hypothalamus of the TNBS-induced experimental colitis rats,which is plausibly one of its action mechanisms in mitigating visceral pain and the pain-related emotions in the experimental colitis rats.

8.
Article in Chinese | WPRIM | ID: wpr-702514

ABSTRACT

Objective To observe the effect of limited laminectomy combined with foraminal stenosis decompression on preventing C5nerve root palsy and improving neurological function. Methods From March,2014 to May,2016,69 patients with multi-segment cervical spondylotic myelopathy underwent surgical treatment in our hospital were included.Thirty-eight patients(group A)were treated with limited lami-nectomy combined with foraminal stenosis decompression and internal fixation,and 31 patients(group B)under-went routine laminectomy and internal fixation.The postoperative neurological recovery rate,cervical curvature index(CCI)and C5palsy rate were recorded and analyzed. Results No spinal cord and nerve injury occurred during the operation.The width of laminectomy was(16.8±2.1)mm in group A,and was significantly less than(21.7±2.5)mm in group B(t=8.849,P<0.001).There was no significant difference in operation time and intraoperative blood loss between two groups(t<0.439,P>0.05).The Japanese Orthopaedic Association (JOA) score increased continuously after surgery in both groups (F>42.996, P<0.05), and no significant difference was found between them at each time point(t<1.021,P>0.05).The cervical curva-ture index improved after surgery(F>86.379,P<0.05),and no significant difference was found between them at each time point(t<0.943,P>0.05).The spinal cord drift distance was(3.6±0.7)mm in group A,and ws signifi-cantly shorter than(2.5±0.5)mm in group B(t=7.602,P<0.001).There was no significantly difference in the neu-rological recovery rate between two groups(t=0.724,P=0.471).The C5palsy rate was lower in group A(5.2%,2/38)than in group B(22.5%,7/31)(χ2=4.514,P=0.034). Conclusion Limited laminectomy combined with foraminal stenosis decompression could relieve the spinal cord com-pression and restrict the excessive back drift, promote the neurological function recovery and reduce the inci-dence of C5palsy.

9.
Tianjin Medical Journal ; (12): 7-11, 2018.
Article in Chinese | WPRIM | ID: wpr-697962

ABSTRACT

Objective To study the effects and mechanisms of BuShenYangGuTang on proliferation and apoptosis of multiple myeloma cell line KM3. Methods The inhibitory effect of BuShenYangGuTang on cell proliferation was assessed by CCK-8. BuShenYangGuTang induced KM3 cell cycle arrest was analyzed by flow cytometry after propidium iodide staining. Flow cytometry was also used to analyze the cell apoptosis after Annexin V-FITC staining. The expressions of Bcl-2, Bax and NF-κB were determined by RT-qPCR and Western blot assay. Results BuShenYangGuTang inhibited the KM3 cell proliferation in a dose-dependent manner. The expression levels of Bcl-2 and NF-κB were decreased, the expression level of Bax was increased, and the cell cycle was arrested in G0 / G1 phase after treatment with BuShenYangGuTang. Conclusion BuShenYangGuTang could inhibit the proliferation, arrest cell cycle and induce the apoptosis in KM3 cells, which may be related to the abnormal expressions of Bcl-2, Bax and NF-κB.

10.
Military Medical Sciences ; (12): 983-986, 2017.
Article in Chinese | WPRIM | ID: wpr-694293

ABSTRACT

Objective To investigate the effect of Astragalus injection on iodine-131(131 I)induced thyroid radiation injury.Methods Two-stage SD male rats were randomly divided into three groups: control group, 131I irradiation group and Astragalus intervention 131I irradiation group.131I irradiation group and Astragalus intervention 131I irradiation group were treated with intragastric administration of 11.1MBq 131I, respectively.At the same time, the Astragalus intervention 131 I irradiation group was injected intraperitoneally 400 mg/(kg· d)Astragalus injection liquid.The levels of thyroid hormone were measured by solid-phase radioimmunoassay in the 2nd and 8th weeks of the experiment.The thyroid tissues from rats were HE stained into paraffin sections after 8 weeks.Administration of 0,25,50,100,200 MBq/ml into 131I irradiation of thyroid follicular carcinoma cells(WRO)lasted 24 hours, the proliferation and apoptosis of WRO in Astragalus membranaceus 0.5 g/L intervention and non-Astragalus intervention were detected by MTT assay and flow cytometry.Results Compared with the normal control group, FT3and FT4were significantly decreased in the 131 I irradiation group(P=0.021,0.017).The morphological changes of the follicular epithelial cells in the thyroid tissue were irregular and the hyaline degeneration was observed.However, compared with 131I irradiation group, FT3and FT4were significantly improved by Astragalus injection(P=0.033,0.045),and the degree of vitreous degeneration of thyroid tissue was alleviated.Cell experiments in vitro showed that the proliferation of thyroid cells was increased, but apoptosis was reduced.Conclusion Astragalus injection can improve the thyroid function and thyroid injury induced by 131 I in rats.

11.
Journal of Experimental Hematology ; (6): 1690-1695, 2017.
Article in Chinese | WPRIM | ID: wpr-278760

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the reversing effect of icaritin on multidrug resistance of multiple myeloma cell lines KM3/BTZ and its underlying mechanism.</p><p><b>METHODS</b>KM3/BTZ cells were established by a gradually ascending gradient induction of bortezomib (BTZ). The sensitivities of KM3 and KM3/BTZ cells to 7 chemotherapeutic drugs, the inhibition and reversal effects of icaritin on proliferation and drug-resistance of KM3/BTZ cells were analyzed by MTT. The apoptosis was analyzed by flow cytometry, and the expression of Par-4, HSP27 and P-gp were detected by Western blot.</p><p><b>RESULTS</b>KM3/BTZ cells were not only resistant to BTZ, but also to other 6 chemotherapeutic drugs. The resistance index (RI) to BTZ was 17.84, and higher than that of other chemotherapeutic drugs. Icaritin inhibited the proliferation and induced the apoptosis of KM3/BTZ cells. The ICvalue of BTZ decreased from 0.345 µg/ml to 0.149 µg/ml, and the reversal index was 2.38 (P<0.05). The expression of Par-4 protein increased in a concentration-dependent manner, while the expression of HSP27 and P-gp were down-regulated.</p><p><b>CONCLUSION</b>Icaritin can inhibit cell proliferation and induce apoptosis of KM3/BTZ cells, moreover, can effectively reverse the multidrug resistance of KM3/BTZ cells. The mechanism may be related with down-regulation of HSP27 and P-gp expression, and up-regulation of Par-4 expression.</p>

12.
Acta Pharmaceutica Sinica ; (12): 1637-1644, 2015.
Article in Chinese | WPRIM | ID: wpr-320030

ABSTRACT

This study was designed to use iTRAQ technology coupled with 2D LC-MS/MS to study the comparative proteomics of different processing technology for pilose antler. 1015 proteins were identified with 2D LC combined with MOLDI TOF/TOF mass spectrometry. Comparative analysis with Protein Pilot (Version 4.5) revealed that 87 proteins were changed (P ≤ 0.05, the ratio of > 1.50 or < 0.60 as the threshold selection of difference proteins), of which 24 were up regulated and 33 were down regulated in the traditional frying process (TFP) compared with the fresh pilose antler (P ≤ 0.05). 7 significant different proteins (P ≤ 0.001), most of these significantly changed proteins were found to be involved in calcium ion binding and ATP binding associated with human healthy. Freeze drying with protective agent (FDP) (Trehalose) can improve the content of significantly different proteins (P ≤ 0.001) including Collagen alpha-1 (XII) chain (COL12A1) and Collagen alpha-1 (II) chain (COL2A1). The significant function involves in platelets activating, maintenance of spermatogonium, and disorder expression in tumor cells. The functional annotation by Hierarchical clustering and GO (gene ontology) showed that the main molecule functions of the proteins significantly changed in these processes were involved in binding (52.7%), catalytic (25.3%), structural molecule and transporter (6.6%).


Subject(s)
Animals , Antlers , Chemistry , Chromatography, Liquid , Collagen , Chemistry , Down-Regulation , Freeze Drying , Gene Expression Regulation , Proteomics , Tandem Mass Spectrometry , Technology, Pharmaceutical , Methods , Up-Regulation
13.
Article in Chinese | WPRIM | ID: wpr-314961

ABSTRACT

There are about 100 species in the genus Coreopsis which distributed in the America, south of Africa and Hawaiian Islands, and 7 species are distributed in China. The inflorescences of Coreopsis tinctoria Nutt. is the Uigur herb 'Snow chrysanthemum' which is named 'Shemuju' with the effects of heat-cleaning, detoxicating, dampness-dissipating and dysentery-curing in the Xinhua Herbal Scheme. The chemical constituents from Coreopsis plants mainly contain flavonoids, phenylpropanoids, sesquiterpenes, and sterols, which show anti-inflammatory activities in modern pharmaceutical research. This article presents an overview of the chemical constituents and pharmaceutical activities, prospects of development and exploitation of Coreopsis plants, hopefully to provide a basis for further research and development of Coreopsis plants.


Subject(s)
Animals , Coreopsis , Chemistry , Drug Discovery , Methods , Drugs, Chinese Herbal , Chemistry , Pharmacology , Humans , Plants, Medicinal , Chemistry
14.
Article in English | WPRIM | ID: wpr-820511

ABSTRACT

OBJECTIVE@#To investigate the effect and the relevant potential mechanism of nonpeptide neurokinin 1 (NK1) receptor antagonist L-703,606 in the edema formation after burn injury.@*METHOD@#L-703,606 treatment was performed in Sprague-Dawley (SD) rats at early stage after deep partial-thickness skin scalding. One hundred and fifty two adult male SD rats were used in the study and randomly divided into sham scald (SS, n=8), scald control (SC, n=48), and L-703,606 treatment (LT, n=48) groups. The rats in SC and LT groups were subjected to 20% total body surface area (TBSA) deep partial-thickness skin scalding. Modified Evans blue extravasation, tracing electron microscopy by lanthanum nitrate and mean water content assay were employed to observe and detect the changes of vascular permeability, ultrastructure and edema formation in adjacent tissue to the wounds and in the jejuna of rats at early stage (72 h) after scald.@*RESULTS@#The pathological increase of vascular permeability in the periwound tissue and jejunum of rats in LT group were significantly lower than that in SC group (P<0.01), and recuperated earlier. Meanwhile, the changes of water contents of corresponding tissues in LT group were lighter than those in SC group (P<0.01). The ultrastructural changes of the microvessels in the peri-wound tissue of LT group showed that the junctions between microvascular endothelium cells were more narrow than those of SC group, moreover, and the number of opening and the engorgement and cavitation of the vascular endothelium cells decreased, the areosis and edema in perivascular tissue lightened, and the precipitation of the high eletron density lanthanum tracing agent in the interspace of the tissue decreased significantly in LT group.@*CONCLUSIONS@#It is concluded that nonpeptide NK1-receptor antagonist L-703,606 could lighten the vascular permeability and edema formation in the periwound tissue and jejunum, and accelerate the normalization process of pathological changes in the tissues of rats after scald.


Subject(s)
Animals , Body Water , Burns , Pathology , Capillary Permeability , Edema , Pathology , Jejunum , Pathology , Male , Microscopy, Electron, Transmission , Neurokinin-1 Receptor Antagonists , Pharmacology , Quinuclidines , Pharmacology , Random Allocation , Rats , Rats, Sprague-Dawley , Receptors, Neurokinin-1 , Metabolism , Skin , Cell Biology , Wounds and Injuries , Pathology
15.
Article in Chinese | WPRIM | ID: wpr-313465

ABSTRACT

<p><b>OBJECTIVE</b>To discuss the origin of occupational stress among petrochemical industry workers and to access the main occupational stressors that impact job satisfaction and mental health of petrochemical industry workers.</p><p><b>METHODS</b>A survey on occupational stressor was carried out by Occupational Stress Indicator (OSI) in 532 petrochemical industry workers (345 chemical and 187 logistic workers).</p><p><b>RESULTS</b>The environment in workplace of chemical group was worse than that of contrast. The chemical workers had less control over job and they experienced more hazards, monotonous as well as role stressors than the logistic group. The scores of job satisfaction and mental health of chemical group (36.867 +/- 0.656, 43.734 +/- 0.542, respectively) were higher than that of contrast (40.321 +/- 0.901, 46.714 +/- 0.745, respectively) (P < 0.05).</p><p><b>CONCLUSION</b>The occupational stressors exist in chemical workers which affect chemical workers' job satisfaction and mental health with different levels.</p>


Subject(s)
Adult , Analysis of Variance , Burnout, Professional , Chemical Industry , Female , Humans , Job Satisfaction , Male , Middle Aged , Petroleum , Regression Analysis , Young Adult
16.
Article in Chinese | WPRIM | ID: wpr-304033

ABSTRACT

<p><b>OBJECTIVE</b>To explore the adverse effects of coking oven emissions (COE) on the serum oxidation-reduction and relevant genes in the exposed workers.</p><p><b>METHODS</b>Fifty-six coke oven workers and forty controls were investigated. Serum Malondialdehyde (MDA) levels and the activities of total superoxide dismutases (T-SOD) were measured by spectrophotometrical method. Immunohistochemical method was used to assess the P21 and P53 levels in peripheral white blood cells.</p><p><b>RESULTS</b>Compared with controls, the individuals exposed to COE had significantly increased levels of serum MDA [(5.30 +/- 2.29) nmol/mL, P < 0.01] and markedly decreased levels of T-SOD [(100.04 +/- 10.75) NU/mL]. Additionally, the median levels of P53 and P21 were markedly increased in the exposed individuals compared with the controls (21.4% and 23.2%, respectively, all P < 0.05).</p><p><b>CONCLUSION</b>The findings indicate that occupational exposure to COE causes the rise of serum oxidation-reduction MDA and the fall of T-SOD, and increasing expression levels of P21 and P53 proteins before the occurrence of apparent clinical symptoms.</p>


Subject(s)
Adult , Air Pollutants, Occupational , Case-Control Studies , Coke , Female , Humans , Lymphocytes , Metabolism , Male , Malondialdehyde , Blood , Middle Aged , Occupational Exposure , Oxidation-Reduction , Proto-Oncogene Proteins p21(ras) , Metabolism , Superoxide Dismutase , Blood , Tumor Suppressor Protein p53 , Metabolism , Young Adult
17.
Neuroscience Bulletin ; (6): 41-46, 2006.
Article in English | WPRIM | ID: wpr-300971

ABSTRACT

Objective To study the protective effect and mechanism of Shuxuetong on gerbil brain tissue from the area of ischemia-reperfusion. Methods Cerebral ischemia-reperfusion animal model has made by transient clipping bilateral common carotid arteries in gerbils. Pathological changes in the hippocampal tissue were observed at different reperfusion time (12h, 3 d, 7 d). The expression levels of GABA and TNF-alpha in the hippocampal CA1 subfield were observed using immunohistochemitry at 12 h, 3 d after reperfusion. The difference of above indices among false operation group, ischemia-reperfusion group and treatment group were compared. Results The injuries of pathology to hippocampal area in ischemia reperfusion group were more serious than treatment group. The expression levels of GABA in treatment group were significantly increased compared with ischemia-reperfusion group, but the expression levels of TNF-alpha between the two groups have no difference. Conclusion Shuxuetong has protective effect on brain tissue of ischemia-reperfusion by enhancing the expression of GABA in the hippocampal tissue.

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