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1.
Article in Chinese | WPRIM | ID: wpr-927390

ABSTRACT

OBJECTIVE@#To compare the efficacy on insomnia between Fang 's scalp acupuncture combined with conventional acupuncture and the simple conventional acupuncture.@*METHODS@#A total of 66 patients with insomnia were randomly divided into an observation group (33 cases, 1 case dropped off) and a control group (33 cases, 2 cases dropped off). In the control group, the routine acupuncture therapy was applied to Shenmen (HT 7), Baihui (GV 20), Zhaohai (KI 6) and Sanyinjiao (SP 6), etc. Based on the treatment as the control group, Fang's scalp acupuncture therapy was supplemented at fuxiang tou, fuzang shangjiao, fuzang zhongjiao, siwei, etc. At these scalp points, the needles were inserted perpendicularly with flying needling technique and manipulated with trembling one. In either group, the treatment was given once daily, continuously for 2 weeks. Before and after treatment, separately, the score of Pittsburgh sleep quality index (PSQI) and the score of Chinese perceived stress scale (CPSS) were observed, as well as the parameters monitored by polysomnography, i.g. total sleep time (TST), sleep onset latency (SOL), wakefulness after the sleep onset (WASO), sleep efficiency (SE), the percentages of the time of rapid eye movement sleep phase (REM) and non-rapid eye movement sleep phase 1, 2, 3 and 4 in TST (REM%, N1%, N2%, N3%). The efficacy was compared between two groups.@*RESULTS@#After treatment, the scores of each factor and the total scores of PSQI, as well as CPSS scores were all lower than those before treatment in the two groups (P<0.01, P<0.05); except the score for sleep quality, the score of each factor and the total score of PSQI, as well as CPSS score in the observation group were lower than those in the control group (P<0.01, P<0.05). After treatment, TST, SE%, REM% and N3% were increased and SOL, WASO, N1% were decreased as compared with before treatment in the two groups (P<0.01, P<0.05), and N2% in the observation group was decreased (P<0.01); SE%, REM% and N3% in the observation group were higher than the control group (P<0.05) and N1% and N2% were lower than the control group (P<0.05). The total effective rate was 93.8% (30/32) in the observation group, higher than 87.1% (27/31) in the control group (P<0.05).@*CONCLUSION@#Fang 's scalp acupuncture, on the base of routine acupuncture, obviously improves the sleep quality and perceived stress and adjusts the sleep structure in the patients with insomnia.


Subject(s)
Acupuncture Points , Acupuncture Therapy/methods , Humans , Scalp , Sleep , Sleep Initiation and Maintenance Disorders/therapy , Stress, Psychological/therapy , Treatment Outcome
2.
Chinese Journal of Immunology ; (12): 251-255, 2018.
Article in Chinese | WPRIM | ID: wpr-702711

ABSTRACT

Objective:To investigate the role and mechanism of IL-38 in inhibiting osteoporosis.Methods:A total of 138 cases of patients with osteoporosis in our hospital from June 2014 to December 2016 were recruited.Another 120 cases of fracture surgery patients without osteoporosis were selected as control.Serum levels of IL-38 in different groups were determined using a commercially available sandwich ELISA (Enzyme-Linked ImmunoSorbent Assay).Construction of IL-38-C57BL/6J transgenic mice,the wild type and IL-38 transgenic mice were set to sham operation group (Sham),operation group (ovariectomy,group OVX) respectively.After 8 weeks of the operation,the serum level of alkaline phosphatase(ALP),calcium and phosphorus were detected.The bilateral femur and spine of mice were collected after sacrifice,the morphology and structure of the femur were analyzed,and the bone density was measured by bone density meter.The bone marrow stromal cells(BMSCs) were isolated and the invitro proliferation ability of BMSCs were meas-ured.Western blot were used to detect the phosphorylation level of PI3K,Akt,GSK3β and NFATc1 in BMSCs.After transfection of IL-38 into mouse osteoblast MC3T3-E1 cell,the phosphorylation level of PI3K,Akt,GSK3β and NFATc1 were detected by Western blot.Apoptosis of MC3T3-E1 cells were detected by flow cytometry.Results:The serum level of IL-38 in patients with osteoporosis were significant lower than control group(P<0.05).The serum level of estrogen,calcium and phosphorus in OVX group of wild type and IL-38 transgenic mice were significant lower than the sham operation group(P<0.05),while the level of ALP was significant higher than sham operation group (P<0.05),but the serum level of calcium and phosphorus in OVX group of IL-38 transgenic mice were significant higher than wild type mice(P<0.05).The pathological section of femur and spine BMD showed that the bone tissue in wild type mice and IL-38 transgenic mice in OVX group were damaged and the bone density decreased significantly,but IL-38 transgenic mice was significant better than wild type mice (P<0.05).The proliferation ability of BMSCs in OVX group of IL-38 transgenic mice was significant higher than wild type mice (P<0.05).The phosphorylation level of PI3K,Akt and NFATc1 in OVX group of IL-38 transgenic mice were significant lower than wild type mice(P<0.05),while the phosphorylation level of GSK3β was significant higher than wild type mice (P<0.05).After transfection of IL-38 into MC3T3-E1 cell,the phosphorylation level of PI3K,Akt and NFATc1 were significant decreased (P<0.05),while the phosphorylation level of GSK3β was significant increased (P<0.05).Flow cytometry assay showed that IL-38 transfection significant decreased the apoptosis of MC3T3-E1 cells(P<0.05).Conclusion:The serum level of IL-38 in patients with osteoporosis is decreased significantly.IL-38 may inhibit the proliferation of BMSCs and inhibit the apoptosis of osteoblasts by regulating the PI3K/Akt/GSK3β/NFATc1 signaling pathway.

3.
Journal of Preventive Medicine ; (12): 869-873, 2017.
Article in Chinese | WPRIM | ID: wpr-792649

ABSTRACT

Objective To evaluate the safety of 60μg recombinant hepatitis B vaccine(Saccharomyces Cerecisiae)in healthy population over 16 years old and immunogenicity in non-responders.Methods A total of 4345 eligible subjects over 16 years old were selected and vaccinated with 60 μg recombinant hepatitis B vaccine, including 3415 participants who have never been vaccined before and 930 non-responders. All participants were monitored for any adverse events occurring within 30 min after each injection and instructed to record selected injection-site reactions and systemic reactions on the day of vaccination and the subsequent 28 days. Blood samples were collected from non-responders at pre-vaccination and one month after vaccination,in order to determine anti-HBs levels,positive rates of anti-HBs and the mean geometric titre(GMT)of anti-HBs.Results Among 4345 vaccinated participants,16.39 % of them reported at least one injection-site or systemic adverse reaction. The most common injection-site and systemic adverse reactions were Grade 1 adverse reactions with the incidence of 15.12 %(657/4345)and 4.05%(176/4345)respectively. No serious adverse events were observed. Among 930 non-responders,the positive rate of anti-HBs was 87.03 % with active responder of 76.74 %(551 / 718)and the GMT of anti-HBs was 479.28 mIU / ml. The positive rate of anti-HBs was not associated with gender or age (P>0.05). The GMT of anti-HBs demonstrated significant differences between female and male(560.66 mIU / mL VS. 404.91 mIU / mL,P<0.05),but there was no significant differences in different age groups (P>0.05).Conclusion 60μg recombinant hepatitis B vaccine was safe for healthy adults above 16 years and had good immunity efficacy among non-responders who had no or low response to standard immunization regimen of hepatitis B vaccine.

4.
Article in English | WPRIM | ID: wpr-287146

ABSTRACT

<p><b>OBJECTIVE</b>To explore the neuroprotective effects of electroacupuncture (EA) on hypoxic-ischemic encephalopathy (HIE) and to further investigate the role of glial cell line-derived neurotrophic factor (GDNF) family receptor member RET (rearranged during transfection) and its key downstream phosphatidylinositol 3 kinase (PI-3K)/protein kinase B (Akt) pathway in the process.</p><p><b>METHODS</b>A total of 220 seven-day-old SD rats (of either sex, from 22 broods) were randomly divided into two groups, one (30 rats) for sham-surgery group and the other (190 rats) for HIE model group. The HIE model was established using the left common carotid artery ligation method in combination with hypoxic treatment. The successfully established rats were randomly divided into five groups, including control model group, EA group, sham-EA group, antagonist group and antagonist plus electroacupuncture group, with 35 rats in each group. Baihui (GV 20), Dazhui (GV 14), Quchi (LI 11) and Yongquan (KI 1) acupoints were chosen for acupuncture. EA was performed at Baihui and Quchi for 10 min once a day for continuous 1, 3, 7 and 21 days, respectively. The rats were then killed after the operation and injured cerebral cortex was taken for the measurement of neurologic damage by hematoxylin-eosin (HE) staining and the degenerative changes of cortical ultrastructure by transmission electron microscopy. RET mRNA level and Akt protein level were detected by real-time reverse-transcription polymerase chain reaction (RT-PCR) and western blot analysis, respectively.</p><p><b>RESULTS</b>EA could ameliorate neurologic damage of the first somatic sensory area (S1Tr) and alleviate the degenerative changes of ultrastructure of cortical neurons in rats subjected to HIE. And the longer acupuncture treatment lasted, the better its therapeutic effect would be. This was accompanied by gradually increased expression of GDNF family receptor RET at the mRNA level and its downstream signaling Akt at the protein level in the ischemic cortex.</p><p><b>CONCLUSION</b>EA has neuroprotective effects on HIE and could be a potential therapeutic strategy for HIE in the neonate. Activation of RET/Akt signaling pathway might be involved in this process.</p>


Subject(s)
Animals , Blotting, Western , Cerebral Cortex , Pathology , Electroacupuncture , Female , Glial Cell Line-Derived Neurotrophic Factor , Genetics , Metabolism , Hypoxia-Ischemia, Brain , Genetics , Pathology , Therapeutics , Male , Nerve Degeneration , Pathology , Neurons , Pathology , Neuroprotective Agents , Therapeutic Uses , Proto-Oncogene Proteins c-akt , Genetics , Metabolism , Proto-Oncogene Proteins c-ret , Genetics , Metabolism , RNA, Messenger , Genetics , Metabolism , Rats, Sprague-Dawley , Real-Time Polymerase Chain Reaction
5.
Chinese Medical Journal ; (24): 2896-2901, 2015.
Article in English | WPRIM | ID: wpr-275598

ABSTRACT

<p><b>BACKGROUND</b>Criteria for determining brain death (BD) vary between China and the United States. We reported the results of an investigation designed to compare procedures to determine BD in two countries.</p><p><b>METHODS</b>The latest criteria in the United states were published in 2010. The latest criteria in China were published in 2009. We used these two types of BD criteria to evaluate patients who were considered to be BD. The time, cost, and accuracy of the diagnosis were compared.</p><p><b>RESULTS</b>From January 1, 2012 to October 8, 2013, there were 37 patients which were applied for BD evaluation in the Neurological Intensive Care Unit of Beijing Tiantan Hospital. The cause of coma were known as subarachnoid hemorrhage (18 patients, 48.6%), intracerebral hemorrhage (8 patients, 21.6%), cerebral ischemia (9 patients, 24.3%), brain stem tumor (1 patient, 2.7%), and intracranial infection (1 patient, 2.7%). The clinical examinations were done for all of the patients except 1 patient who had low blood pressure. Three patients had brainstem reflexes that were excluded from BD. Twenty-five patients had apnea tests, and 20 tests were completed that were all positive. Confirmatory tests were completed differently: Transcranial Doppler (30 patients, positive rate 86.7%), electroencephalogram (25 patients, positive rate 100%), and somatosensory evoked potential (16 patients, positive rate 100%). Thirty-three patients were diagnosed BD by criteria of the United States. Only 9 patients were diagnosed BD by Chinese criteria. The use of time and money in the USA criteria was obviously fewer than those in Chinese criteria (P = 0.000).</p><p><b>CONCLUSION</b>Compared with BD criteria of the United States, Chinese criteria were stricter, lower positive rate, more cost in money and time, and more reliable by families and doctors.</p>


Subject(s)
Aged , Brain Death , Diagnosis , China , Female , Humans , Male , Middle Aged , United States
6.
Chinese Medical Journal ; (24): 1236-1241, 2013.
Article in English | WPRIM | ID: wpr-342198

ABSTRACT

<p><b>BACKGROUND</b>The mortality of stroke patients is strongly affected by medical complications. However, there are limited data investigating the effect of in-hospital medical complications on the dependency of stroke patients worldwide. We prospectively and systematically investigated the effect of medical complications on dependency of patients at 3, 6 and 12 months after stroke using the China National Stroke Registry (CNSR).</p><p><b>METHODS</b>This prospective cohort study collected data of patients age > 18 years with acute ischemic stroke in 132 clinical centers distributed across 32 provinces and four municipalities (including Hong Kong region) of China, from September 2007 to August 2008. Data on medical complications, dependency and other information were obtained from paper-based registry forms. Medical complications associated with stroke outcomes were assessed using multivariable Logistic regression.</p><p><b>RESULTS</b>Of 11 560 patients with acute ischemic stroke, 1826 (15.80%) presented with in-hospital medical complications. In-hospital medical complications were independent risk factors for dependency of patients at 3 months (adjusted odds ratio (OR) 2.367, 95% confidence interval (CI) 2.021 - 2.771), 6 months (adjusted OR 2.257, 95%CI 1.922 - 2.650), and 12 months (adjusted OR 1.820, 95%CI 1.538 - 2.154) after acute ischemic stroke.</p><p><b>CONCLUSION</b>The results demonstrated that the short-term and long-term dependency of acute ischemic stroke patients is significantly associated with in-hospital medical complications in China.</p>


Subject(s)
Aged , Brain Ischemia , Epidemiology , China , Female , Humans , Male , Middle Aged , Prospective Studies , Registries , Stroke , Epidemiology
7.
Chinese Medical Journal ; (24): 2449-2454, 2012.
Article in English | WPRIM | ID: wpr-283742

ABSTRACT

<p><b>BACKGROUND</b>In-hospital medical complications are associated with poorer clinical outcomes for stroke patients after disease onset. However, few studies from China have reported the effect of these complications on the mortality of patients with acute ischemic stroke. In this prospective work, the China National Stroke Registry Study, we investigated the effect of medical complications on the case fatality of patients with acute ischemic stroke.</p><p><b>METHODS</b>From September 2007 to August 2008, we prospectively obtained the data of patients with acute stroke from 132 clinical centers in China. Medical complications, case fatality and other information recorded at baseline, during hospitalisation, and at 3, 6, and 12 months after stroke onset. Multivariable Logistic regression was performed to analyze the effect of medical complications on the case fatality of patients with acute ischemic stroke.</p><p><b>RESULTS</b>There were 39 741 patients screened, 14 526 patients with acute ischemic stroke recruited, and 11 560 ischemic stroke patients without missing data identified during the 12-month follow-up. Of the 11 560 ischemic patients, 15.8% (1826) had in-hospital medical complications. The most common complication was pneumonia (1373; 11.9% of patients), followed by urinary tract infection and gastrointestinal bleeding. In comparison with patients without complications, stroke patients with complications had a significantly higher risk of death during their hospitalization, and at 3, 6 and 12 months post-stroke. Having any one in-hospital medical complication was an independent risk factor for death in patients with acute ischemic stroke during hospital period (adjusted OR = 6.946; 95%CI 5.181 to 9.314), at 3 months (adjusted OR = 3.843; 95%CI 3.221 to 4.584), 6 months (adjusted OR = 3.492; 95%CI 2.970 to 4.106), and 12 months (adjusted OR = 3.511; 95%CI 3.021 to 4.080). Having multiple complications strongly increased the death risk of patients.</p><p><b>CONCLUSION</b>Short-term and long-term outcomes of acute stroke patients are affected by in-hospital medical complications.</p>


Subject(s)
Aged , China , Female , Gastrointestinal Hemorrhage , Hospital Mortality , Hospitalization , Humans , Male , Middle Aged , Pneumonia , Prospective Studies , Registries , Stroke , Mortality , Urinary Tract Infections
8.
Article in English | WPRIM | ID: wpr-328477

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effects of electroacupuncture (EA) on the structure parameters of synapse and reactive changes of astrocyte in the marginal zone of focal cerebral ischemia in rats at different time zones so as to further explore its underlying mechanisms in the treatment of cerebral ischemia.</p><p><b>METHODS</b>Ninety male Wistar rats were randomly assigned to sham-operation, model, and EA groups, with 30 animals in each group. Each group was subdivided into 1 h, as well as 1, 3, 7, and 21 days post-operation groups, with 6 animals assigned to each time point subgroup. Heat coagulation-induced occlusion of the middle cerebral artery was performed to establish a model of focal cerebral ischemia. EA was applied immediately following surgery to the EA group [4/20 Hz, 2.0-3.0 V, 1-3 mA, to Baihui (GV20) and Dazhui (GV14)] for 30 min. Treatment was performed once a day, and experimental animals were sacrificed at 1 h, as well as 1, 3, 7 and 21 days postoperation. The ultrastructure changes in synapse and astrocytes were observed by using transmission electron microscopy. Glial fibrillary acidic protein (GFAP) expression and Ca(2+) of astrocytes were measured by using laser confocal scanning microscope. Excitatory amino acid transporters-2 (EAAT2) and connexin 43 (CX43) expressions were assayed with immunohistochemical method. Canonical correlation analysis was conducted between structure parameters of synapse and parameters of astrocyte in the same time and group.</p><p><b>RESULTS</b>Broken synapses were observed following cerebral ischemia, and the numbers of synapses were significantly decreased. Compared with the model group, synaptic ultrastructure was significantly improved in the EA group. Compared with the sham-operation group, synaptic number density was significantly decreased, as were postsynaptic density thickness, synaptic cleft width and synaptic interface curvature in the EA and model groups. However, compared with the model group, postsynaptic density thickness was significantly increased in the EA group at the same time points post-operation (P <0.05, P<0.01). In addition, synaptic cleft width, synaptic number density and synaptic interface curvature were significantly increased with the passage of time (P <0.05, P<0.01). The expression of GFAP in the EA group were significantly lower than those in the model group at all the time points (P <0.05, P<0.01). OD values of EAAT2 in the EA group were significantly higher than those in the model group at the same time (P <0.05, P<0.01). Compared with that in the model group, the expressions of CX43 in the EA group increased significantly at 3 days and 7 days (P <0.05, P<0.01). Ca(2+) average fluorescence intensity of astrocytes in the EA group was significantly lower than those in the model group at 1 h, 1 day, 3 days and 7 days (P <0.05, P<0.01). The changes in structure parameters of synapse were closely related to the changes of CX43, EAAT2, GFAP, Ca(2+) of astrocytes by EA treatment at all the time points.</p><p><b>CONCLUSIONS</b>EA is helpful for synaptic reorganization, which may be related to its effect on intervening the activation state of astrocytes and promoting the beneficial interaction between astrocytes and synapses. Acupuncture could start the adjustment of neuron-glial network so as to promote the synaptic reorganization, which may be the key mechanism of treating cerebral ischemia.</p>


Subject(s)
Animals , Astrocytes , Metabolism , Pathology , Brain Ischemia , Pathology , Therapeutics , Calcium , Metabolism , Connexin 43 , Metabolism , Electroacupuncture , Methods , Excitatory Amino Acid Transporter 2 , Metabolism , Fluorescence , Glial Fibrillary Acidic Protein , Metabolism , Male , Post-Synaptic Density , Metabolism , Rats , Rats, Wistar , Statistics as Topic , Synapses , Metabolism , Pathology
9.
Chinese Medical Journal ; (24): 3640-3643, 2010.
Article in English | WPRIM | ID: wpr-336570

ABSTRACT

<p><b>BACKGROUND</b>The development of diabetic cardiomyopathy is multifactorial. Insulin resistance (IR) and excessive activity of the renin-angiotensin system are confirmed reasons for diabetic cardiomyopathy. Renin-angiotensin system (RAS) inhibitors can reduce tissue Ang II levels, with beneficial effects on cardiovascular function. Therefore, in type-2 diabetes mellitus (T2DM), blockade of the RAS may have the function of protecting against diabetic cardiomyopathy through increasing insulin sensitivity and inhibiting excessive activity of RAS. However, this has not been confirmed.</p><p><b>METHODS</b>The effect of valsartan, an angiotensin receptor blocker (ARB), on diabetic cardiomyopathy in the presence of T2DM was studied. Wistar rats with T2DM and T2DM treated with valsartan were studied. Glucose infusion rates (GIR), index of IR, heart weight, the heart weight-to-body weight ratio (HW/BW), myocardial apoptotic index, cardiac hydroxyprolin content, and cardiac tissue collagen type I and collagen type III content were measured.</p><p><b>RESULTS</b>GIR in T2DM rats and T2DM rats treated with valsartan decreased (P < 0.01). In T2DM rats treated with valsartan, heart weight, myocardial apoptotic index, cardiac hydroxyprolin content, and cardiac tissue collagen type I and collagen type III content were higher than in control rats, but lower than in T2DM rats. In rats with T2DM, GIR was negatively and significantly correlated with all the variables. However, in T2DM rats treated with valsartan or normal control rats, none of the correlations was significant.</p><p><b>CONCLUSIONS</b>In the presence of T2DM, diabetic cardiomyopathy is related with IR. Valsartan can not alleviate IR, but can protect against diabetic cardiomyopathy and remove the correlation between IR and diabetic cardiomyopathy.</p>


Subject(s)
Angiotensin II Type 1 Receptor Blockers , Therapeutic Uses , Animals , Apoptosis , Collagen Type I , Collagen Type III , Diabetes Mellitus, Type 2 , Drug Therapy , Metabolism , Diabetic Cardiomyopathies , Hydroxyproline , Insulin Resistance , Male , Myocardium , Chemistry , Pathology , Rats , Rats, Wistar , Tetrazoles , Therapeutic Uses , Valine , Therapeutic Uses , Valsartan
10.
Asian Journal of Andrology ; (6): 157-165, 2009.
Article in English | WPRIM | ID: wpr-284681

ABSTRACT

We investigated the effects of transient receptor potential M8 (TRPM8) channel on the proliferation and motility of androgen-independent prostate cancer PC-3 cells. After being permanently transfected with an empty vector and cDNA encoding the TRPM8 protein, cells were analysed for cell cycle distribution and motility using flow cytometry and scratch assay. Immunocytochemistry and Ca2+ imaging analysis revealed the overexpression of functional TRPM8 channel on both endoplasmic reticulum and plasma membrane of PC-3-TRPM8 cells. Cell cycle distribution and scratch assay analysis revealed that TRPM8 induced cell cycle arrest at the G0/G1 stage (P < 0.05) and facilitated the cell apoptosis induced by starvation (P < 0.05). Furthermore, TRPM8 inhibited the migration of PC-3-TRPM8 cells (P < 0.01) through the inactivation of focal-adhesion kinase. It appears that TRPM8 was not essential for the survival of PC-3 cells; however, the overexpression of TRPM8 had negative effects on the proliferation and migration of PC-3 cells. Thus, TRPM8 and its agonists may serve as important targets for the treatment of prostate cancer.


Subject(s)
Adenocarcinoma , Genetics , Metabolism , Pathology , Apoptosis , Calcium , Metabolism , Cell Cycle , Cell Line, Tumor , Cell Movement , Physiology , Cell Proliferation , Cell Transformation, Neoplastic , Cytosol , Metabolism , DNA-Binding Proteins , Genetics , Metabolism , Epithelial Cells , Metabolism , Pathology , Focal Adhesion Protein-Tyrosine Kinases , Gene Expression Regulation, Neoplastic , Humans , Male , Prostatic Neoplasms , Genetics , Metabolism , Pathology , Starvation , Pathology , TRPM Cation Channels , Genetics , Metabolism , Transcription Factors , Genetics , Metabolism , Transfection
11.
Article in Chinese | WPRIM | ID: wpr-321743

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the expression of transient receptor potential melastatin (TRPM) and transient receptor potential vanilloid (TRPV) channel family genes in rat spermatogenic cells.</p><p><b>METHODS</b>Rat spermatogenic cells were isolated by a mechanical procedure and the total RNA was extracted using TRIzol reagent. TRPM and TRPV channel family genes were amplified by RT-PCR and the presence of the target genes was detected by agarose gel electrophoresis. The relative gene expression levels were measured by real-time quantitative RT-PCR.</p><p><b>RESULTS</b>TRPV5, TRPM3, TRPM4 and TRPM7 mRNAs were expressed in rat spermatogenic cells, but TRPV1, TRPV2, TRPV3, TRPV4, TRPV6, TRPM1, TRPM2, TRPM5, TRPM6, TRPM7 and TRPM8 mRNAs were not detected. The relative expressions of TRPM and TRPV mRNA were determined by quantitative real-time RT-PCR. TRPM7 expression was the highest among all the TRPM subtypes in rat spermatogenic cells, at a level equivalent to (0.0430-/+0.0034)% of beta-actin expression. TRPM3 and TRPM4 were also highly expressed, but their expression levels were only approximately 56% and 63% of that of TRPM7, respectively. For the TRPV subfamily, only TRPV5 mRNA was abundantly expressed at the level of (0.0157-/+0.0029)% relative to that of beta-actin.</p><p><b>CONCLUSION</b>TRPV5, TRPM3, TRPM4 and TRPM7 mRNAs were coexpressed in spermatogenic cells in rats, among which TRPM4 and TRPM7 mRNA were expressed at high levels. TRPM4 and TRPM7 channels may be involved in the regulation of growth, differentiation and maturation of rat spermatogenic cells and are associated with the generation of the sperms.</p>


Subject(s)
Animals , Male , RNA, Messenger , Genetics , Metabolism , Rats , Rats, Sprague-Dawley , Spermatocytes , Cell Biology , Metabolism , Spermatogonia , Cell Biology , Metabolism , TRPM Cation Channels , Genetics , Metabolism , TRPV Cation Channels , Genetics , Metabolism
12.
National Journal of Andrology ; (12): 690-694, 2008.
Article in Chinese | WPRIM | ID: wpr-309813

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the best dose and the long-term effect of the human insulin-like growth factor-1 (hIGF-1) gene injection into the penis of aged rats.</p><p><b>METHODS</b>Included in this study were 10 young (4 months old) and 40 aged (24 months old) Sprague-Dawley male rats, the latter equally divided into a PBS control and a 10 microg, a 100 microg and a 1 000 microg hIGF-1 injection group. Electrical stimulation was conducted 4 and 8 weeks after hIGF-1 injection into the penile corpus cavernous of the rats to detect the intracavernous pressure (ICP) and mean arterial pressure (MAP). Dose - and time -associated therapeutic results were analyzed and the mRNA expression of hIGF-1 determined by RT - PCR.</p><p><b>RESULTS</b>ICP, MAP and total ICP were significant decreased by electrical stimulation in the aged rats as compared with the young ones (P < 0.05), statistically increased in the three hIGF-1 dose groups in comparison with the PBS controls (P < 0.05), and showed no obvious difference between the young rats and the latter two dose groups at 4 and 8 weeks. Although less obvious effect was achieved in the 10 microg group than in the young rats, the therapeutic result was still of significance. The mRNA expression of the hIGF-1 gene was confirmed in all the hIGF-1 treated rats.</p><p><b>CONCLUSION</b>The hIGF-1 therapy can improve erectile function in aged rats, 100 microg suffices for effective erection and the effect may last at least 8 weeks for a single dose.</p>


Subject(s)
Aging , Physiology , Animals , Dose-Response Relationship, Drug , Erectile Dysfunction , Therapeutics , Genetic Therapy , Methods , Insulin-Like Growth Factor I , Genetics , Physiology , Male , Penis , Metabolism , Plasmids , Genetics , RNA, Messenger , Genetics , Metabolism , Rats , Rats, Sprague-Dawley , Reverse Transcriptase Polymerase Chain Reaction , Time Factors
13.
Chinese Medical Journal ; (24): 852-855, 2004.
Article in English | WPRIM | ID: wpr-284893

ABSTRACT

<p><b>BACKGROUND</b>Stroke unit is the most effective treatment method to benefit stroke patients. Our study is to evaluate the early effectiveness of a hospital stroke unit (SU).</p><p><b>METHODS</b>Three hundred and ninety-two patients who had suffered from acute strokes and who were admitted to our hospital between December 2001 and January 2003 were recruited for this controlled study. All patients were sent at random to either the SU or the general ward (GW) for treatment. The following indices were measured by: Barthel Index (BI), National Institute of Health Stroke Scale (NIHSS), Oxford Handicap Scale (OHS).</p><p><b>RESULTS</b>The mean change in BI score between the day of admission and the day of discharge was 20.00 +/- 24.36 for the SU group and 10.63 +/- 23.59 for the GW group. A difference that is statistically significant (P = 0.000). The mean change in NIHSS score was -2.01 +/- 6.61 for the SU group and 0.55 +/- 7.44 for the GW group. A difference that is also statistically significant (P = 0.000). Finally, the mean change in OHS score was -0.74 +/- 1.04 for the SU group and -0.28 +/- 0.98 for the GW group, also a statistically significant difference (P = 0.000). Among SU patients, patient satisfaction was higher (P = 0.000), the rehabilitation success rate was higher (P = 0.000), and there were fewer complications (P = 0.000).</p><p><b>CONCLUSION</b>Compared to GW patients, stroke patients treated in a special SU were able to return to normal daily activities earlier, with better social abilities, and have reduced neurological defects, without increasing the overall economic burden.</p>


Subject(s)
Evaluation Studies as Topic , Hospital Units , Humans , Stroke , Mortality , Stroke Rehabilitation
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