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1.
Article in Chinese | WPRIM | ID: wpr-862704

ABSTRACT

<b>Objective::To study the chemical constituents of pericarps of <italic>Zanthoxylum bungeanum</italic>. <b>Method::The dried pericarps of <italic>Z</italic>. <italic>bungeanum</italic> were smashed, and then extracted and concentrated in 95%ethanol to obtain the total extract. The total extract was loaded on a silica gel CC, eluted with different polar solvents in sequence, and then concentrated to obtain corresponding parts. The methylene chloride fraction and the <italic>n</italic>-butanol fraction were separated and purified by silica gel column chromatography, Sephadex LH-20 gel column chromatography, ODS column chromatography, semi-preparative HPLC, etc. And their structures were identified based on physicochemical properties and various spectroscopic methods. <b>Result::Fourteen compounds were isolated from the dichloromethane fraction and the n-butanol fraction of the <italic>Z</italic>. <italic>bungeanum</italic> and identified as(1<italic>S</italic>, 3<italic>S</italic>)-1-methyl-1, 2, 3, 4-tetrahydro-<italic>β</italic>-carboline-3-carboxylic acid(<bold>1</bold>), (3<italic>S</italic>)-1, 2, 3, 4-tetrahydro-<italic>β</italic>-carboline-3-carboxylic acid(<bold>2</bold>), apigenin-7, 4′-dimethyl ether(<bold>3</bold>), genkwanin(<bold>4</bold>), lcariside F<sub>2</sub>(<bold>5</bold>), breyniaionoside A(<bold>6</bold>), 3-methoxyphenethyl alcohol-4-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopynanoside(<bold>7</bold>), 1-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopyranosyloxy-3-methoxy-5-hydroxybenzene(<bold>8</bold>), orcinol-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>9</bold>), syringin(<bold>10</bold>), 4-[(3<italic>S</italic>)-3-hydroxybutyl]-2-methoxyphenyl-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>11</bold>), (+ )-lyoniresinol-3a-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>12</bold>), 2-methylpropanyl-6-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-apiofuranosyl-<italic>β</italic>-<italic>D</italic>-glucopyranoside(<bold>13</bold>)and(<italic>E</italic>)-6-hydroxy-2, 6-dimethylocta-2, 7-dienoic acid(<bold>14</bold>). <b>Conclusion::All compounds were isolated from <italic>Z</italic>. <italic>bungeanum</italic> for the first time, and compounds <bold>1-4, 12</bold> and <bold>14</bold> were isolated from this genus for the first time.

2.
Article in Chinese | WPRIM | ID: wpr-773738

ABSTRACT

In order to study the interaction between Pterocephalus hookeri and bitter taste receptors,three-dimensional structural models of bitter taste receptors TAS2 R16,TAS2 R14 and TAS2 R13 were established by homology modeling in this paper. Maestro software was used for docking the chemical constituents of P. hookeri with bitter taste receptors. The results showed that 25 chemical components of P. hookeri can regulate three bitter taste receptors. And these components were mainly iridoid glycosides and phenolic acids.This research focused on the comprehensive application of homology modeling and molecular docking technology to explore the interaction between bitter chemical constituents of P. hookeri and bitter taste receptors. This study provided assistance in revealing pharmacodynamic basis of bitter Tibetan medicine at molecular level. It also provided new ideas and methods for the study of Tibetan medicine.


Subject(s)
Caprifoliaceae , Chemistry , Correlation of Data , Humans , Medicine, Tibetan Traditional , Molecular Docking Simulation , Receptors, G-Protein-Coupled , Metabolism , Taste
3.
Article in Chinese | WPRIM | ID: wpr-773736

ABSTRACT

The intestinal absorption characteristics of ten iridoid glycosides and phenolic acids in the Pterocephali Herba were evaluated via rat intestinal valgus model. The intestinal sac fluids at different time after administration of high,medium and low concentrations of Pterocephali Herba extract were collected and ten chemical components in fluid samples were detected by UPLC-PDA. Accumulative absorbed doses( Q) and absorption rate constants( Ka) of ten chemical constituents were calculated,while proportions between Pterocephali Herba extract and intestinal absorption liquid were compared. The results showed that the intestinal absorption of 10 chemical components was linear absorption( R2>0. 9) at different concentrations,which accorded with the zero-order absorption rate. The absorption rate constant was related to the concentration of the drug and the intestinal site,which indicated that intestinal adsorption mechanism of the components were passive diffusion and active transport. Proportions of chemical constituents in intestinal sac fluid were different from those in Pterocephali Herba extract. Therefore,those ten chemical components in Pterocephali Herba extract can be absorbed in whole intestine. Everted intestinal sac model can be used to evaluate intestinal absorption characteristics of ingredients in Pterocephali Herba extract effectively.


Subject(s)
Animals , Caprifoliaceae , Chemistry , Drugs, Chinese Herbal , Pharmacokinetics , Intestinal Absorption , Intestines , Plant Extracts , Pharmacokinetics , Rats , Rats, Sprague-Dawley
4.
Article in Chinese | WPRIM | ID: wpr-802256

ABSTRACT

Objective: To study the potential anti-inflammatory active ingredient of Chinese herbal Polygoni Cuspidati Rhizoma et Radix. Method: The inflammatory model of peritoneal macrophages (RAW264.7 cells) induced by lipopolysaccharide (LPS) in mice was used to screen out the anti-inflammatory activity of 95% ethanol extract of Polygoni Cuspidati Rhizoma et Radix and its macroporous resin elution site (30%, 60%, 95% ethanol eluting site). The characteristic fingerprints were established by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) technology, and then partial least squares method (partial least squares, PLS) was used to study the spectrum-effect relationship between the peak area of the characteristic components and the inhibition rate of nitric oxide (NO), and potential anti-inflammatory active ingredients were identified according to variable important in projection (VIP). Result: The ethanol extract macroporous resin 60% ethanol elution site of Polygoni Cuspidati Rhizoma et Radix had the strongest inhibition ability of nitric oxide formation, with a certain dose-dependent relationship. The study of spectrum-effect relationship showed that 3 components had potential anti-inflammatory activity, namely Emodin-8-O-β-D-glucoside (E-8-G), Emodin-1-O-β-D-giucoside, and Emodin-8-O-(6'-O-malonyl)-glucoside. In addition, the anti-inflammatory activity of E-8-G was further validated at the cell level through molecular docking analysis. Conclusion: Three potential anti-inflammatory active ingredients were found base on the spectrum-effect relationship. This study strategy is helpful to find the active ingredients (group) of traditional Chinese medicine, and provides new research ideas and methods for studying the material basis of Chinese herbal medicine.

5.
Article in Chinese | WPRIM | ID: wpr-798510

ABSTRACT

Objective:To study the chemical constituents of 95% ethanol extract of the whole plant of Elephantopus scaber and investigate its antioxidant activity. Method:The chemical constituents were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography,ODS column chromatography,semi-preparative high performance liquid chromatography (HPLC) and recrystallization methods,while their structures were identified on the basis of nuclear magnetic resonance (NMR), mass spectrometry and comparison of their spectral data with those reported in the literature. Result:Fourteen compounds were isolated and their structures were identified as:(E)-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)-2(3H)-furanone (1),esculetin (2),dihydrosyringenin (3),(+)-isololiolide (4),caffic acid (5),luteolin-7-O-β-D-glucuronide (6),luteolin-7-O-β-D-glucuronide methyl ester (7),chlorogenic acid methyl ester (8),blumenol A (9),(6R,9R)-3-oxo-α-ionol-β-D-glucopyranoside (10),byzantionoside B (11),3,5-O-dicaffeoyl quinic acid methyl ester (12),3,4-O-dicaffeoyl quinic acid methyl ester (13) and 4,5-O-dicaffeoyl quinic acid methyl ester (14). Conclusion:Compounds 1-4 and 8-10 were isolated from the genus Elephantopus for the first time. Compound 11 was isolated from E. scaber for the first time. The DPPH radical and ABTS+ radical scavenging experiments on twelve of these compounds showed that compounds 1,2,5,6,12 and 13 had significant antioxidant activity.

6.
Article in Chinese | WPRIM | ID: wpr-693374

ABSTRACT

Obiective To investigate the chemical constituents of Pothos chinensis.Methods Compounds were isolated by the silica gel and Sephadex LH-20,semi-PHPLC,preparative thin layer chromatography and recrystallization.Structures of the com-pounds were identified by the physicochemical properties as well as the 1H NMR,13C NMR and MS spectroscopic data.Results Six-teen compounds were isolated from the whole plant of P.chinensis and their structures were identified as liquiritigenin(1),p-hydroxy-benzoic acid(2),syringic acid(3),E-4-hydroxycinnamic acid(4),N-trans-p-coumaroyltyramine(5),N-trans-cinnamoyltyramine (6),N-trans-feruloyltyramine(7),(+)-epiloliolide(8),neoechinulin A(9),vitexin(10),berchemolide(11),(±)-syringaresinol (12),prunasin(13),4-hydroxy-3,5-dimethoxybenzaldehyde(14),β-sitosterol(15)and β-daucosterol(16),respectively.Conclu-sion Compounds 1 and 9-14 are isolated from the genus Pohos for the first time.

7.
Article in Chinese | WPRIM | ID: wpr-687373

ABSTRACT

Toad venom (Chansu) is prepared from the dried secretion of parotid gland and skin gland from Bufo bufo gargarizans or B. melanostictus. Up to now, much attention shall be paid to the poor quality of commercial toad venom because of the adulteration. So, it is urgent to establish a scientific and perfect quality control method to improve the quality of toad venom and guarantee its safety and effectiveness in clinical application. The different batches of toad venom samples were assayed by high performance liquid chromatography (HPLC) and the quantitative analysis of multi-components by single marker (QAMS) was used to detect the contents of five bufagenins. As a result, the reference characteristic chromatogram was established, displaying serotonin, gamabufotalin, arenobufagin, hellebrigenin, telocinobufagin, bufotalin, cinobufotalin, bufalin, cinobufagin and resibufogenin as characteristic peaks. Taking cinobufagin as an internal reference substance, QAMS was verified for the determination of five bufagenins (gamabufotalin, bufotalin, bufalin, cinobufagin, resibufogenin) in toad venom samples. The durability and applicability of the relative correction factor (RCF) were also studied systematically. RCFs of cinobufagin to gamabufotalin, bufotalin, bufalin and resibufogenin were determined as 1.05, 0.895, 1.09 and 0.913, respectively. The characteristic chromatogram and QAMS established in this study could effectively control the quality of toad venom and provide scientific evidence for the improvement of the quality standard of the toad venom to be described in Chinese Pharmacopoeia (2020 edition).

8.
Article in English | WPRIM | ID: wpr-812113

ABSTRACT

The present study was designed to investigate the chemical constituents of the whole herb of Dichrocephala benthamii. A new megastigmane glucoside (compound 1), together with its four known analogues (compounds 2-5), was obtained. Their structures were elucidated on the basis of spectroscopic analyses (UV, IR, MS, and 1D and 2D NMR). The absolute configuration of compound 1 was assigned on the basis of CD method and chemical evidence. In addition, their cytotoxicity against human hepatoma cells (HepG-2) was evaluated by the MTT method. Compound 5 showed weak activity against HepG-2, while the other compounds did not show remarkable inhibitory effects.


Subject(s)
Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Asteraceae , Chemistry , China , Cyclohexanones , Chemistry , Pharmacology , Drug Screening Assays, Antitumor , Drugs, Chinese Herbal , Chemistry , Glucosides , Chemistry , Pharmacology , Hep G2 Cells , Humans , Molecular Structure , Norisoprenoids , Chemistry , Pharmacology , Plants, Medicinal
9.
Article in Chinese | WPRIM | ID: wpr-256047

ABSTRACT

Fourteen compounds were isolated from the 80% ethanol extract of Caragana stenophylla root, by using a combination of various chromatographic approaches, including silica gel sephadex LH-20 column chromatography, and preparative HPLC. On the basis of their physical and chemical properties and spectroscopic data, their structures were elucidated as 2-(4-hydroxy-3-methoxy lphenyl)-3-methoxyl benzofuran-6-ol (1), mucodianin C (2), isopterofuran (3), formononetin (4), afromosin (5), calycosin (6), acacetin (7), 3-O-methylkaempferol (8), liquiritigenin (9), isoliquiritigenin (10), variabilin (11), resveratrol (12), zhebeiresinol (13), and 2, 3-dicarboxy-6, 7-dihydroxy-1-(3', 4'-dihydroxy)-phenyl-1, 2-dihydronaphthalen (14). Compound 1 is a new benzofuran derivative, named as mucodianin S; compounds 2, 3, 11, 13, 14 were isolated from the genus Caragana for the first time, and compounds 4-10 were firstly isolated from Caragana stenophylla. MTT assay was used to determine their cytotoxicity of the isolated compounds against human tumor cell lines, and 2 showed cytotoxicity against human hepato cellular cancer (HepG2) and human cervical (HeLa) lines, with IC₅₀ values of (16.18±0.95), (3.75±0.08) μmol•L ⁻¹, respectively.

10.
Article in Chinese | WPRIM | ID: wpr-350197

ABSTRACT

This study is to develop an UPLC-PDA method for determination of 10 major components in Pterocephalus. The UPLC-PDA assay was performed on a Waters Acquity UPLCR BEH C₁₈(2.1 mm ×100 mm,1.7 μm), and the column temperature was at 30 ℃. The mobile phase consists of water containing 0.2% phosphoric acid (A) and acetonitrile (B) in gradient elution at a flow rate of 0.4 mL•min⁻¹. The detection wave length was set at 237 and 325 nm, and the injection volume was 1 μL in the UPLC system. The linear range of 10 detected compounds were good (r≥0.999 7), and the overall recoveries ranged from 96.30% to 103.0%, with the RSD ranging from 0.72% to 2.9%. The method was simple, accurate and reproducible, which can be used for the simultaneous determination of the content of ten major components in P. hookeri.

11.
Article in Chinese | WPRIM | ID: wpr-337969

ABSTRACT

The triterpenoids of Dichrocephala benthamii were investigated by means of silica gel, Sephadex LH-20 and semi-preparative HPLC. Nine triterpenoids were isolated from D. benthamii. By analysis of the EI-MS, NMR spectra and comparison to the data reported in literatures, the structures of these compounds were determined as β-amyrin formiate (1), β-amyrin acetate (2), β-amyrenol (3), β-amyrone (4), 3β-hydroxy-olean-11, 13 (18)-diene (5) , Δ12-oleanene (6) , friedelin (7), dammaradienyl acetate (8), epi-friedeband (9), respectively. Compounds 1-8 were isolated for the first time form this genus, compound 9 was isolated for the first time from this plant, whereas β-amyrin formiate (1) was a new natural product.


Subject(s)
Asteraceae , Chemistry , Triterpenes , Chemistry
12.
Article in Chinese | WPRIM | ID: wpr-310973

ABSTRACT

Asteraceae Echionopos (Echinops genus) is one of the world's plants commonly used in traditional Chinese medicine herbs. In this paper, a lot of literature on the basis of domestic and foreign, and modern research on the application of medicinal plants in different ethnic groups in our country Echionopos made a brief presentation and evaluation, and its in Chinese Han, Mongolian, Uygur, Kazak, Korean and other ethnic groups in the application of traditional medicine and related research conducted profiles. To further comprehensive and accurate use of the abundant plant resources and research, in order to find biologically active natural products, provide reference basis for the clinical application of new drugs developed and expanded drug source.


Subject(s)
Animals , Asteraceae , Chemistry , Drug Therapy , Drugs, Chinese Herbal , Chemistry , Pharmacology , Humans , Medicine, Chinese Traditional
13.
Article in Chinese | WPRIM | ID: wpr-283854

ABSTRACT

<p><b>OBJECTIVE</b>To study the chemical constituents of the rhizomes of Smilax china.</p><p><b>METHOD</b>The constituents of the rhizomes of S. china were isolated and purified by repeated silica gel and Sephadex LH-20 chromatography, and their structures were elucidated on the basis of spectral analysis.</p><p><b>RESULT</b>Thirteen compounds were obtained and identified as kaemperol-7-O-beta-D-glucopyranoside (1), engeletin (2), isoengeletin (3), kaempferol (4), dihydrokaempferol (5), dihydrokaempferol-5-O-P-D-glucopyranoside (6), rutin (7), kaempferol- 5-O-beta-D-glucopyranoside (8), 3, 5, 4'-trihydroxystibene (9), vanillic acid (10), 3, 5-dimethoxy4-O-beta-D-glu-copyranosylcinnamic acid (11), beta-sitosterol (12), and beta-daucosterol (13) , respectively.</p><p><b>CONCLUSION</b>Compounds 1, 3, 7, 8, and 11 were isolated from this plant for the first time, and compounds 8 and 11 were isolated from the genus Smilax for the first time.</p>


Subject(s)
Chromatography , Flavonoids , Chemistry , Flavonols , Chemistry , Glycosides , Chemistry , Kaempferols , Chemistry , Magnetic Resonance Spectroscopy , Plants, Medicinal , Chemistry , Rhizome , Chemistry , Sitosterols , Chemistry , Smilax , Chemistry , Vanillic Acid , Chemistry
14.
Article in Chinese | WPRIM | ID: wpr-252213

ABSTRACT

<p><b>OBJECTIVE</b>To study the chemical constituents of the stems of Clematis parviloba.</p><p><b>METHOD</b>The compounds were isolated and purified by repeated column chromatography with silica gel, Sephadex LH-20 and HPLC. Their structures were identified by spectroscopic data together with physical and chemical property.</p><p><b>RESULT</b>Ten compounds have been isolated from the stems of C. parviloba, and identified as: (+) pinoresionol (1), (+) pinoresionol-4'-O-p-D-glucopyranoside (2), ( +) pinoresionol4, 4'-O-bis-beta-D-glucopyranoside (3), (-) syringaresinol (4), (+) syringaresinol-4'-O-beta-D-glucopyranoside (5), (-)episyringaresinol (6), (+) medioresinol-4'-O-beta-D-glucopyranoside (7), (+) lariciresinol-4-O-beta-D-glucopyranoside (8), (+) lariciresinol-4'-O-beta-D-glucopyranoside (9), (+) lariciresinol-4, 4'-O-bis-beta-D-glucopyranoside (10), respectively.</p><p><b>CONCLUSION</b>Compounds 6, 7 were isolated from this genus for the first time, and the other ones were isolated from this plant for the first time.</p>


Subject(s)
Chromatography, Gel , Chromatography, High Pressure Liquid , Clematis , Chemistry , Drugs, Chinese Herbal , Chemistry , Furans , Chemistry , Glucosides , Chemistry , Lignans , Chemistry , Magnetic Resonance Spectroscopy , Plant Stems , Chemistry , Spectrometry, Mass, Electrospray Ionization
15.
Acta Pharmaceutica Sinica ; (12): 233-235, 2006.
Article in English | WPRIM | ID: wpr-271469

ABSTRACT

<p><b>AIM</b>To investigate the glycosidic constituents in the rhizomes of Alpinia officinarum Hance.</p><p><b>METHODS</b>The isolation and purification of glycosides were done with column chromatography on macro porous resin, polyamides and Sephadex LH-20, whilst the structure elucidation was done by HRCI-MS and NMR (1D and 2D) methods.</p><p><b>RESULTS</b>A glycosidic ester identified as 4'-hydroxy-2'-methoxyphenol-beta-D-{6-0-[4"-hydroxy-3", 5"-dimethoxy (benzoate)]}-glucopyranoside (I), along with a known compound n-butyl-beta-D-fructopyranoside (II), were isolated and characterized.</p><p><b>CONCLUSION</b>I was found to be a new compound, named as alpinoside A, whilst II was isolated from the genus Alpinia for the first time.</p>


Subject(s)
Alpinia , Chemistry , Fructose , Chemistry , Glucosides , Chemistry , Molecular Conformation , Molecular Structure , Plants, Medicinal , Chemistry , Rhizome , Chemistry
16.
Article in Chinese | WPRIM | ID: wpr-279131

ABSTRACT

<p><b>OBJECTIVE</b>To determine the best processing technology parameters.</p><p><b>METHOD</b>The changes of the weights, volatile constituents, total extract quantities by MeOH or CHCl3 and curcumenol contents of the roots of Curcuma wenyujin and C. kwangsiensis roots before and after water soaking were determined, so as to determine the quality control index for the process of preparing Yujin slices. The shortest soaking and moistening time and the appropriate drying temperature were studied.</p><p><b>RESULT AND CONCLUSION</b>(1) The weight was decreased and the quantities of volatile constituents were changed during water soaking process. But the total extracts quantities and the curcumenol contents didn't change obviously. So the quality control index was decided as soaking as short as possible. (2) The soaking time could be decreased by using high pressure. The soaking time was related with the short diameter of the cross section of the root. (3) The soaking under pressure technology is that soaking on -0.095 MPa for 0.5 h, then on 0.14 MPa for 10-16 h, and moistening for 36-48 h, cutting drying in room temperature or less than 40 degrees C.</p>


Subject(s)
Curcuma , Chemistry , Classification , Oils, Volatile , Plant Extracts , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Pressure , Quality Control , Technology, Pharmaceutical , Methods , Time Factors , Water
17.
Acta Pharmaceutica Sinica ; (12): 536-538, 2005.
Article in Chinese | WPRIM | ID: wpr-353478

ABSTRACT

<p><b>AIM</b>To study the benzophenones in the roots of Securidaca inappendiculata Hassk.</p><p><b>METHODS</b>Column chromatography (including silica gel and Sephadex LH-20) was used to isolate benzophenones whose structures were elucidated by HREI-MS, NMR (1D and 2D) methods.</p><p><b>RESULTS</b>A new benzophenone was isolated and identified as 2-methoxy-3,4-methylenedioxy-benzophenone (I), along with a known compound 4-hydroxy-2,6-dimethoxy-benzophenone (II).</p><p><b>CONCLUSION</b>Compound I is a new one named as securiphenone B, compound II was isolated from the genus for the first time.</p>


Subject(s)
Benzophenones , Chemistry , Molecular Conformation , Molecular Structure , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Securidaca , Chemistry
18.
Acta Pharmaceutica Sinica ; (12): 722-725, 2004.
Article in Chinese | WPRIM | ID: wpr-302728

ABSTRACT

<p><b>AIM</b>To study the triterpenoid saponins in the whole plants of Lysimachia capillipes Hemsl..</p><p><b>METHODS</b>Column chromatography (including AB-8 macroporous resin, silica gel and ODS) was used to separate triterpenoid saponins whose structures were elucidated by ESI-MS, NMR (1D and 2D) and hydrolysis methods.</p><p><b>RESULTS</b>Two new triterpenoid saponins were isolated and established as 3beta, 22alpha-dihydroxy-16alpha-angeloyloxy-28-->13-lactone-oleanane-3-O-[beta-D-glucopyranosyl-(1-->2)-alpha-L-arabinpyranosyl]-22-O-(6-acetyl)-beta-D-glucopyranoside (I), 3beta, 13beta, 22alpha-trihydroxy-16alpha-acetyloxyoleanane-28-oic acid Na-3-O-[beta-D-glucopyranosyl-(1-->2)-alpha-L-arabinpyranosyl]-22-O-beta-D-glucopyranoside (II).</p><p><b>CONCLUSION</b>Compounds I and II are new compounds and named as capilliposide I and capilliposide J.</p>


Subject(s)
Flavonoids , Chemistry , Molecular Conformation , Molecular Structure , Plants, Medicinal , Chemistry , Primulaceae , Chemistry , Triterpenes , Chemistry
19.
Acta Pharmaceutica Sinica ; (12): 726-729, 2004.
Article in Chinese | WPRIM | ID: wpr-302727

ABSTRACT

<p><b>AIM</b>To develop a capillary gas chromatographic method for the determination and pharmacokinetic study of patchouli alcohol in rat plasma after iv administration.</p><p><b>METHODS</b>The drug was extracted with ethyl acetate. Eugenol was used as internal standard. The separation was carried out on a HP-5MS quartz capillary column, with high-purity nitrogen as carrier gas and flame ionization detector (FID) as detector. The column temperature was maintained at 80 degrees C for 1 min and then programmed to 200 degrees C at a rate of 15 degrees C x min(-1); it was held at 200 degrees C for 1 min, and then programmed to 290 degrees C at a rate of 60 degrees C x min(-1); the final temperature was held for 1 min. The temperature of both injector and detector was set at 290 degrees C.</p><p><b>RESULTS</b>The standard curve was linear from 25 to 5 000 microg x L(-1) in rat plasma. The recovery of this method was from 90.0% to 110.0% with satisfactory relative standard deviation (RSD) less than 10.0%. The pharmacokinetic parameters demonstrated patchouli alcohol were consistent with the two-compartment open model and showed linear pharmacokinetics. The T1/2beta, AUC and MRT of patchouli alcohol in patchouli oil were all higher than that of patchouli alcohol.</p><p><b>CONCLUSION</b>This method is quick, precise and reliable. The pharmacokinetics of patchouli alcohol is different from that of patchouli alcohol in patchouli oil.</p>


Subject(s)
Animals , Area Under Curve , Injections, Intravenous , Lamiaceae , Chemistry , Male , Oils, Volatile , Chemistry , Plants, Medicinal , Chemistry , Rats , Rats, Sprague-Dawley , Sesquiterpenes , Blood , Pharmacokinetics
20.
Acta Pharmaceutica Sinica ; (12): 194-197, 2004.
Article in Chinese | WPRIM | ID: wpr-301116

ABSTRACT

<p><b>AIM</b>To study the triterpenoid saponins in the whole plant of Lysimachia davurica Ledeb.</p><p><b>METHODS</b>Column chromatography (including AB-8 macroporous resin, silica gel and ODS) was used to separate triterpenoid saponins whose structures were elucidated by ESI-MS, NMR (1D and 2D) and hydrolysis methods.</p><p><b>RESULTS</b>Two new triterpenoid saponins were isolated and established as 3beta, 16alpha, 28-trihydroxy-olean-12-en-3-0-(beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucuronopyranosyl)-28-0-beta-D-glucopyranoside) (I), 3beta, 16alpha, 28-trihydroxy-olean-12-en-3-O-(beta-D-glucopyranosyl-(1 --> 2)-beta-D-glucuronopyranosyl)-28-0-beta-D-glucopyranoside) (II).</p><p><b>CONCLUSION</b>Compounds I and II are new compounds and named as davuricosides D and J.</p>


Subject(s)
Molecular Conformation , Molecular Structure , Plants, Medicinal , Chemistry , Primulaceae , Chemistry , Saponins , Chemistry , Triterpenes , Chemistry
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