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1.
Article in Chinese | WPRIM | ID: wpr-315795

ABSTRACT

<p><b>OBJECTIVE</b>To explore the relationship among the expression of GSK3β, PI3K/Akt signaling transduction pathway,and cytokines IL6 in Chronic Rhinosinusitis.</p><p><b>METHODS</b>We assayed mRNA and protein for GSK3β, PI3K, Akt by using reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry (IHC), and measured the cytokines IL6 mRNA by using reverse transcription polymerase chain reaction (RT-PCR) in the nasal tissue from the patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP), Chronic Rhinosinusitis without Nasal Polyps (CRSsNP) and control subjects.</p><p><b>RESULTS</b>The relative expression levels of GSK3β, PI3K, Akt and IL-6 in CRSwNP were 0.6254 ± 0.0584, 0.8239 ± 0.7186, 0.9369 ± 0.0823 and 0.8973 ± 0.0680. But the relative expression levels of GSK3β, PI3K, Akt and IL-6 in control subjects were 0.2684 ± 0.0726, 0.3578 ± 0.0994, 0.6721 ± 0.0590, 0.5898 ± 0.0891. There were significant differences between the groups of CRSwNP and control subjects (t values were 2.358, 3.071, 2.764, and 2.239, respectively, all P < 0.05). There was no significant difference of GSK3β, PI3K, Akt and GSK3β between the groups of CRSwNP and CRSsNP (t values were 1.597, 1.842, 1.468 and 0.926, respectively, all P < 0.05). GSK3β, PI3K, Akt were not only expressed in the cytoplasm of the epithelium and gland cells, but also in the cytoplasm of the inflammatory cell. GSK3β, PI3K, Akt protein in CRS were detected at a higher rate than the normal nasal tissue (values were 16.42, 16.25 and 15.57,respectively, all P < 0.01). However there was no significant difference of GSK3β, PI3K, Akt protein between the groups of CRSwNP and CRSsNP (values were 3.27, 2.85 and 2.46, respectively, all P > 0.05). There was a positive correlation trend among the expression of GSK3β, PI3K and Akt, and IL-6 in CRS (r values were 0.645, 0.617 and 0.583,respectively, all P < 0.01).</p><p><b>CONCLUSIONS</b>The abnormal expression of IL-6, PI3K, Akt and GSK3β in the nasal mucosa of CRS may play a pro-inflammatory role in the occurrence and development of CRS.</p>


Subject(s)
Adolescent , Adult , Case-Control Studies , Chronic Disease , Female , Glycogen Synthase Kinase 3 , Metabolism , Glycogen Synthase Kinase 3 beta , Humans , Interleukin-6 , Metabolism , Male , Middle Aged , Nasal Mucosa , Metabolism , Phosphatidylinositol 3-Kinases , Metabolism , Proto-Oncogene Proteins c-akt , Metabolism , Signal Transduction , Sinusitis , Metabolism , Young Adult
2.
Article in Chinese | WPRIM | ID: wpr-262492

ABSTRACT

<p><b>OBJECTIVE</b>To observe the microstructural changes of olfactory mucosa in rat model with acute rhinosinusitis leading to olfactory dysfunction, and to provide foundation for further exploration of corresponding mechanism.</p><p><b>METHODS</b>On the basis of prior successfully established rat model of acute rhinosinusitis through inoculation with Streptococcus pneumoniae and with the help of merocel strips, one hundred healthy SD rats were randomly divided into experimental group (80) and control group (20). After inoculation, every 20 rats in the experimental groups were sacrificed in first week, second week, third week and fourth week respectively; and all rats in the control group were sacrificed in first week after the inoculation. Before the rats were sacrificed, the method called "buffed food pellet test, BFPT" was adopted, which was advanced by professor Nathan, to measure the rats' olfaction,and the time of every rat spending in finding out the food pellet was recorded and analyzed. BFPT showed that the rats in experimental group spent (402.9 ± 9.3), (453.7 ± 7.3), (351.9 ± 8.9), (278.7 ± 8.1) s respectively in searching the food pellet, which were more than the rats in the control group [(178.3 ± 6.6) s]. Then the olfactory mucosa was collected under anatomic microscope from all the rats to make frozen section and detect the changes of mature olfactory receptor neurons (ORN) and olfactory ensheathing cells (OEC) by immunofluorescence technique.</p><p><b>RESULTS</b>The reduction of ORN in various degrees could be detected in the tissue samples of olfactory mucosa among all the rats in experimental group, with a tendency to become thinner in the thickness of epithelial lamina during the inflammation developing course. This kind of pathology was most marked in the second week and it gradually developed into the stage showing the lesion being the feeblest in the forth week following the beginning of modeling. Although the number of olfactory ensheathing cells appeared reduction in the first week following the beginning of modeling as well,it came to increase from the second week before olfactory receptor neurons and almost completely recovered to normal in the fourth week. In addition, some olfactory ensheathing cells could be detected in the tissue samples of olfactory mucosa among all the rats in experimental group.</p><p><b>CONCLUSIONS</b>Both mature olfactory sensory neurons and olfactory ensheathing cells appeared to reduction when sinonasal mucosa taken place acute rhinosinusitis. But the number of olfactory ensheathing cells increased faster than olfactory sensory neurons. In addition, some olfactory ensheathing cells could be detected in the olfactory epithelium.</p>


Subject(s)
Animals , Disease Models, Animal , Male , Olfaction Disorders , Pathology , Olfactory Mucosa , Olfactory Receptor Neurons , Rats , Rats, Sprague-Dawley , Rhinitis , Pathology , Sinusitis , Pathology , Smell
3.
Article in Chinese | WPRIM | ID: wpr-318878

ABSTRACT

<p><b>OBJECTIVE</b>To explore the role of monocyte chemoattractant protein-1 (MCP-1) in lupus nephritis (LN).</p><p><b>METHODS</b>Sera MCP-1 levels were measured by enzyme linked immunosorbent assay in 112 patients with systemic lupus erythematosus (SLE), 30 patients with rheumatoid arthritis, 11 non-SLE patients with renal impairment, and 40 healthy volunteers. MCP-1 mRNA expression in peripheral blood mononuclear cells (PBMCs) was also investigated with reverse trancription-polymerase chain reaction semi-quantitative method.</p><p><b>RESULTS</b>The expression of MCP-1 was significantly higher in active LN groups than in all other groups (P < 0.001), and there was a close correlation between MCP-1 expression and the overall SLE disease activity index score (r=0.6245, P < 0.001) and the SLE disease activity index renal score (r=0.6808, P < 0.001). Low expression of MCP-1 was observed in diseased controls and healthy controls. The sera levels of MCP-1 were significantly higher in patients with active diseases than in patients with inactive SLE and controls, but no significant difference were found between the active LN groups and non-renal involvement group (P >0.05).</p><p><b>CONCLUSION</b>The expression of PBMCs MCP-1 mRNA is upregulated in active SLE. Meanwhile, its expression levels are correlated with the activity of LN.</p>


Subject(s)
Adolescent , Adult , Aged , Arthritis, Rheumatoid , Metabolism , Chemokine CCL2 , Genetics , Female , Humans , Lupus Erythematosus, Systemic , Metabolism , Lupus Nephritis , Metabolism , Male , Middle Aged , RNA, Messenger , Genetics
4.
Article in Chinese | WPRIM | ID: wpr-231941

ABSTRACT

<p><b>OBJECTIVE</b>To explore the role of eotaxin in the pathogenesis of bronchial asthma and the clinical value in the diagnosis of asthma.</p><p><b>METHODS</b>Serum eotaxin were measured by ELISA in 38 patients with asthma, 28 patients with non-asthma allergy, and 30 healthy controls.</p><p><b>RESULTS</b>The levels of serum eotaxin in the asthma group were higher than those in the non-asthma allergic and control group (P<0.01). Furthermore, eotaxin levels in patients with acute asthma were significantly higher than those in patients with stable asthma (P<0.001). It was also found that the eotaxin levels of the acute asthma group were positively correlated to the amounts of eosinophils in peripheral blood (r=0.4196, P<0.001), and inversely correlated to the forced expiratory volume in one second (FEV1) (r=-0.3746, P<0.001).</p><p><b>CONCLUSION</b>It suggests that eotaxin may play a crucial pathogenic role in the asthmatic process possibly by activating the allergic inflammatory cells and controlling the recruitment of eosinophils from blood to bronchial epithelium of the airway. The concentration of eotaxin is significantly associated with the attack of acute asthma and its severity. Eotaxin may be a potential therapeutic target in patients with asthma.</p>


Subject(s)
Adult , Asthma , Diagnosis , Cell Count , Chemokine CCL11 , Chemokines, CC , Blood , Physiology , Eosinophilia , Pathology , Female , Forced Expiratory Volume , Humans , Male , Middle Aged
5.
Article in Chinese | WPRIM | ID: wpr-685149

ABSTRACT

Objective To explore the relation between the expression of PBMCs IP-10 mRNA and systemic lupus erythematosus.Methods The expression of PBMCs IP-10 mRNA was investigated by RT-PCR semi quantitative method and samples from 46 patients with SLE,20 patients with RA,11 non-SLE patients with renal impairment and 20 healthy volunteers.Results The expression of PBMCs IP-10 mRNA in active SLE group was significantly higher than that in inactive group(P0.05).Serum levels of IP-10 were highly correlated with the expression levels of PBMCs IP-10 mRNA(r=0.897 1,P

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