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Article in Chinese | WPRIM | ID: wpr-274358


<p><b>OBJECTIVE</b>To explore the once sampling quantitation of Houttuynia cordata through its DNA polymorphic bands that carried information entropy, from other form that the expression of traditional Chinese medicine polymorphism, genetic polymorphism, of traditional Chinese medicine.</p><p><b>METHOD</b>The technique of inter simple sequence repeat (ISSR) was applied to analyze genetic polymorphism of H. cordata samples from the same GAP producing area, the DNA genetic bands were transformed its into the information entropy, and the minimum once sampling quantitation with the mathematical mode was measured.</p><p><b>RESULT</b>One hundred and thirty-four DNA bands were obtained by using 9 screened ISSR primers to amplify from 46 strains DNA samples of H. cordata from the same GAP, the information entropy was H=0.365 6-0.978 6, and RSD was 14.75%. The once sampling quantitation was W=11.22 kg (863 strains).</p><p><b>CONCLUSION</b>The "once minimum sampling quantitation" were calculated from the angle of the genetic polymorphism of H. cordata, and a great differences between this volume and the amount from the angle of fingerprint were found.</p>

Amplified Fragment Length Polymorphism Analysis , DNA, Plant , Genetic Variation , Houttuynia , Genetics , Microsatellite Repeats , Genetics
Article in Chinese | WPRIM | ID: wpr-251251


<p><b>OBJECTIVE</b>To establish and experimental verification the mathematical model of the balance groups that is the steady-state of traditional Chinese medicine in extraction.</p><p><b>METHOD</b>Using the entropy and genetic principles of statistics, and taking the coefficient of variation of GC fingerprint which is the naphtha of the Houttuynia cordata between strains in the same GAP place as a pivot to establish and verify the mathematical model was established of the balance groups that is the steady-state of traditional Chinese medicine in extraction.</p><p><b>RESULT</b>A mathematical model that is suitable for the balance groups of the steady-state of traditional Chinese medicine and preparation in extraction, and the balance groups which is 29 683 strains (approximately 118.7 kg) were gained with the same origin of H. cordata as the model drug.</p><p><b>CONCLUSION</b>Under the GAP of quality control model, controlling the stability of the quality through further using the Hardy-Weinberg balance groups of the H. cordata between strains, the new theory and experiment foundation is established for the steady-state of traditional Chinese medicine in extraction and quality control.</p>

Chemical Fractionation , Methods , Chromatography, Gas , Drugs, Chinese Herbal , Reference Standards , Houttuynia , Chemistry , Genetics , Models, Statistical , Quality Control