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Article in Chinese | WPRIM | ID: wpr-665512


Objective To understand the epidemiological trend of imported malaria and its monitoring and control effect in Yancheng City from 2011 to 2015,so as to provide the evidence for adjusting the prevention and control strategy. Methods The data of malaria surveillance,epidemic,prevention and control were collected and analyzed in the districts and counties of Yancheng City from 2011 to 2015. Results From 2011 to 2015,there were 104 imported malaria cases reported,and the most cases(52 cases,50%)were reported from Jianhu County. Most of the cases were males(only 2 females),the average age was 38.8 years,and the occupation was mainly labor service worker abroad(94 cases,97%). The infection source of these cases mainly came from Africa. From 2011 to 2015,151980 fever patients received malaria blood tests,and the positive rate was 0.07%. Falciparum malaria cases were the most(90 cases,86.5%). The confirmed diagnostic rate of malaria within 24 hours in-creased year by year. All the 104 patients received the standardized treatment. Conclusion There are no local malaria cases in Yancheng City from 2011 to2015,and in the next stage,the monitoring and prevention should be focused on imported malaria, including shortening the confirmed diagnostic time and improving the diagnostic efficiency.

Article in Chinese | WPRIM | ID: wpr-250526


<p><b>OBJECTIVE</b>To compare the molecular characteristics of hantaviruses isolated from Shandong province by using PCR typing and nucleotide sequencing.</p><p><b>METHODS</b>Total cellular RNA was extracted from hantaviruses infected Vero E6 cells, viral cDNA was amplified by polymerase chain reaction. PCR products of partial M Segments of 4 strains of hantaviruses were sequenced. Cross neutralization tests were performed.</p><p><b>RESULTS</b>Four strains of hantavirus isolated from Rottus in Shandong province were SEO like viruses. The homology between Shandong isolates and other SEO like viruses was high at both amino acid and nucleotide levels. The homology among those 4 strains of hantaviruses was 98%.</p><p><b>CONCLUSIONS</b>The SEO like hantaviruses were more conserved than other hantaviruses. The homology of SEO like hantaviruses isolated from Shandong province was as high as 98% at nucleotides level, though they were isolated at more than 10-year intervals.</p>

Animals , Base Sequence , China , DNA, Viral , Disease Reservoirs , Genetic Variation , Hantaan virus , Classification , Genetics , Hemorrhagic Fever with Renal Syndrome , Virology , Humans , Molecular Sequence Data , Phylogeny , Polymerase Chain Reaction , Sequence Homology, Nucleic Acid