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Objective To perform microscopic identification for the roots of Actinidia macrosperma C.F. Liang, Actinidia valvata Dunn, Actinidia arguta (Sieb. & Zucc) Planch. ex Miq., Actinidia chinensis Planch., and provide the basis for judging medicinal materials exactly. Methods The powder microscopic characteristics of 4 medicinal plants of Actinidia genus were observed by microscopic identification method. Results Taking the morphological characteristics of calcium oxalate clusters, starch granules and ducts as the main differences, a key table was compiled to identify the roots of these four medicinal plants. Conclusion The microscopic identification method could effectively distinguish 4 Chinese herbs of Actinidia genus, and which is worth further studying.
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Objective: Compare and analyze the results of the domestic Lanyi AH600 glycated hemoglobin analyzer and other different detection systems to understand the comparability of the detection results of different detectors, and establish the best cut point of Lanyi AH600 determination of haemoglobin A1c (HbA1c) in the diagnosis of diabetes. Methods: Multi center cohort study was adopted. The clinical laboratory departments of 18 medical institutions independently collected test samples from their respective hospitals from March to April 2022, and independently completed comparative analysis of the evaluated instrument (Lanyi AH600) and the reference instrument HbA1c. The reference instruments include four different brands of glycosylated hemoglobin meters, including Arkray, Bio-Rad, DOSOH, and Huizhong. Scatter plot was used to calculate the correlation between the results of different detection systems, and the regression equation was calculated. The consistency analysis between the results of different detection systems was evaluated by Bland Altman method. Consistency judgment principles: (1) When the 95% limits of agreement (95% LoA) of the measurement difference was within 0.4% HbA1c and the measurement score was≥80 points, the comparison consistency was good; (2) When the measurement difference of 95% LoA exceeded 0.4% HbA1c, and the measurement score was≥80 points, the comparison consistency was relatively good; (3) The measurement score was less than 80 points, the comparison consistency was poor. The difference between the results of different detection systems was tested by paired sample T test or Wilcoxon paired sign rank sum test; The best cut-off point of diabetes was analyzed by receiver operating characteristic curve (ROC). Results: The correlation coefficient R2 of results between Lanyi AH600 and the reference instrument in 16 hospitals is≥0.99; The Bland Altman consistency analysis showed that the difference of 95% LoA in Nanjing Maternity and Child Health Care Hospital in Jiangsu Province (reference instrument: Arkray HA8180) was -0.486%-0.325%, and the measurement score was 94.6 points (473/500); The difference of 95% LoA in the Tibetan Traditional Medical Hospital of TAR (reference instrument: Bio-Rad Variant II) was -0.727%-0.612%, and the measurement score was 89.8 points; The difference of 95% LoA in the People's Hospital of Chongqing Liang Jiang New Area (reference instrument: Huizhong MQ-2000PT) was -0.231%-0.461%, and the measurement score was 96.6 points; The difference of 95% LoA in the Taihe Hospital of traditional Chinese Medicine in Anhui Province (reference instrument: Huizhong MQ-2000PT) was -0.469%-0.479%, and the measurement score was 91.9 points. The other 14 hospitals, Lanyi AH600, were compared with 4 reference instrument brands, the difference of 95% LoA was less than 0.4% HbA1c, and the scores were all greater than 95 points. The results of paired sample T test or Wilcoxon paired sign rank sum test showed that there was no statistically significant difference between Lanyi AH600 and the reference instrument Arkray HA8180 (Z=1.665,P=0.096), with no statistical difference. The mean difference between the measured values of the two instruments was 0.004%. The comparison data of Lanyi AH600 and the reference instrument of all other institutions had significant differences (all P<0.001), however, it was necessary to consider whether it was within the clinical acceptable range in combination with the results of the Bland-Altman consistency analysis. The ROC curve of HbA1c detected by Lanyi AH600 in 985 patients with diabetes and 3 423 patients with non-diabetes was analyzed, the area under curve (AUC) was 0.877, the standard error was 0.007, and the 95% confidence interval 95%CI was (0.864, 0.891), which was statistically significant (P<0.001). The maximum value of Youden index was 0.634, and the corresponding HbA1c cut point was 6.235%. The sensitivity and specificity of diabetes diagnosis were 76.2% and 87.2%, respectively. Conclusion: Among the hospitals and instruments currently included in this study, among these four hospitals included Nanjing Maternity and Child Health Care Hospital in Jiangsu Province (reference instrument: Arkray HA8180), Tibetan Traditional Medical Hospital of TAR (reference instrument: Bio-Rad Variant Ⅱ), the People's Hospital of Chongqing Liang Jiang New Area (reference instrument: Huizhong MQ-2000PT), and the Taihe Hospital of traditional Chinese Medicine in Anhui Province (reference instrument: Huizhong MQ-2000PT), the comparison between Lanyi AH600 and the reference instruments showed relatively good consistency, while the other 14 hospitals involved four different brands of reference instruments: Arkray, Bio-Rad, DOSOH, and Huizhong, Lanyi AH600 had good consistency with its comparison. The best cut point of the domestic Lanyi AH600 for detecting HbA1c in the diagnosis of diabetes is 6.235%.
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Pregnancy , Child , Humans , Female , Glycated Hemoglobin , Cohort Studies , Diabetes Mellitus/diagnosis , Sensitivity and Specificity , ROC CurveABSTRACT
Based on the ancient Chinese medical literature and modern clinical application literature， Mahuangtang was systematically analyzed， including its origin， composition， processing requirements and others， in order to provide support for the development of this famous classical formula. It was found that there were some doubts about Mahuangtang， such as the original variety， the processing of decoction pieces， and the dose conversion ratio. On the basis of fully considering the actual needs of the development of famous classical formula preparations and the usage habits of modern clinical practice， the development suggestions of the author were as follows：Ephedrae Herba should be selected Ephedra sinica without removing knots， Cinnamomi Ramulus should be selected from Cinnamomum cassia， Glycyrrhizae Radix et Rhizoma should select Glycyrrhiza uralensis and be roasted with honey， and Armeniacae Semen Amarum should be selected as Dan-processed products. The total dose of Mahuangtang was 24 g after conversion according to 1 Liang equal to 3 g as following the transition phenomenon and law. The preparation and usage method was as follows：took 1.8 L of water， first added Ephedrae Herba in water for decocting， evaporated 400 mL of water， removed the upper foam， then added the other three drugs， cooked over gentle heat to 500 mL， filtered the residue， took three times a day， about 160 mL warm decoction once. The major function recorded of Mahuangtang in ancient books was exterior sthenia syndrome of exogenous wind cold， it often used in the clinical treatment of cold， acute bronchitis， bronchial asthma and other diseases through the flexible application of the later doctors.
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Liangmianzhen(Zanthoxyli Radix) has long been used as medicine. The current medicinal parts are different from those in the ancient. As recorded in the Chinese Pharmacopeia, the medicinal part is root. However, in ancient works, the medicinal parts include root, stem, leaf, and fruit. In an attempt to find the historical basis that stem is a reasonable medicinal part, the herbalogical study was carried out on this medicinal based on the formal names, synonyms, original plant, medicinal parts, habitat of the medicinal plant, producing area, processing and preparation methods, efficacy, and indications recorded in ancient Chinese materia medica and local gazetteers. The results showed that Liangmianzhen was firstly recorded as a medicinal in Shennong's Classic of Materia Medica with the formal name of "Manjiao". "Manjiao" was adopted from the Han Dynasty to the Qing Dynasty when it was changed to "Rudijinniu", the name originating from the folk in the south of the Five Ridges. Now, the formal name is "Liangmianzhen", which was firstly recorded in Wuxuan County Gazetteer in 1914 and then as a synonym in the Updated Records of Picking Herbs in the South of the Five Ridges. According to the formal names, synonyms, and the descriptions of the original plant, the medicinal plants of Liang-mianzhen have the characteristics of shrub-like young seedlings, vine adult seedlings, corymbiform thyrsus, stems with thorns, amphitropous golden-yellow roots with horn-like branches, and thorns on both sides of the leaves. Thus, "Manjiao", "Rudijinniu", and "Liangmianzhen" were from the same species of Zanthoxylum nitidum(Rutaceae), which was also verified based on the growth environment, habitat, processing and preparation methods, efficacy, and indications. In ancient times, the stem and root were the main medicinal parts and leaves and fruits were also used. However, in the Chinese Pharmacopeia, root is recorded as the only medicinal part, which is obviously inconsistent with the records in the ancient classics. In light of the limited medicinal resources for Liang-mianzhen, other medicinal parts of Z. nitidum is recommended. This study clarified the medicinal parts of Z. nitidum in history. It is recommended that the stem be added to the medicinal parts of Z. nitidum in the next edition of Chinese Pharmacopeia.
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China , Drugs, Chinese Herbal , Fruit , Materia Medica , Medicine, Chinese Traditional , Plants, MedicinalABSTRACT
Objective: To study the chemical constituents in the stems of Trigonostemonlutescens. Methods:Silica gel column chromatography and semi-preparative high performance liquid chromatography were used for separation and purification. The structures were identified by NMR, IR, MS and other spectral techniques. Toxicity of compounds 1-4 on human lung cancer A549 cells, cervical cancer Hela cells and breast cancer MCF-7 cells was determined by the MTT method. Results: Four lignans were isolated from the stems of T. lutescens. The structures were identified as 5,5'-dimethoxylclemaphenol A (1), syringaresinol (2), lirioresinol B dimethyl ether (3) and clemaphenol A (4). Conclusion:Compound1 is a new compound, and compounds 2-4 are isolated from this genus for the first time. Compounds 1-4 have certain cytotoxic activity on A549, HeLa and MCF-7 human tumor cells.
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The prescription research and clinical application of Wenjingtang were summarized in order to provide reference for the formulation of material standard and the development of compound preparation. By systematically sorting out the relevant ancient medical books and modern literature reports， combined with the relevant policy requirements of the development of compound preparations of famous classical formulas， this paper expounded the existing problems and put forward some suggestions. Wenjingtang is composed of nine herbs， which is derived from Complete Effective Prescriptions for Women's Diseases written by CHEN Zi-ming in Song dynasty. The original medicinal plants and medicinal parts of the formula were basically the same as those recorded in the 2020 edition of Chinese Pharmacopoeia， for example， Paeonia lactiflora was the choice of Paeoniae Radix Alba， Cinnamomi Cortex was selected as Guixin， the rhizoma of Curcuma phaeocaulis was selected as Curcumae Rhizoma. It was suggested that raw products should be selected for decoction pieces and processed according to the methods recorded in the 2020 edition of Chinese Pharmacopoeia. According to the dosage of 1 Liang=40 g， the dosages of Angelicae Sinensis Radix， Chuanxiong Rhizoma， Paeoniae Radix Alba， Cinnamomi Cortex， Moutan Cortex and Curcumae Rhizoma were 20 g， the dosages of Ginseng Radix et Rhizoma， Glycyrrhizae Radix et Rhizoma and Achyranthis Bidentatae Radix were 40 g， the total amount of this formula was 240 g. The 9 kinds of decoction pieces were crushed （particle size<6 mm） separately and mixed， each dose was 20 g， 525 mL of water was added， and then decocted to 280 mL. After filtration， warm medicine was taken for once a day. According to ancient books， Wenjingtang has the functions of activating blood circulation， regulating menstruation，warming meridians and dispersing cold. It is mainly used to treat dysmenorrhea， irregular menstruation and other diseases in modern clinical practice. In addition， it has certain curative effect on endometriosis， pelvic inflammatory disease， acne， eczema and other skin diseases. Through the research， the historical evolution and clinical application of Wenjingtang are fully clarified， which can provide research for the later development and application of this famous classical formula.
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OBJECTIVE@#To observe the effects of acupotomy intervention on the behavior, morphology and tensile mechanics of knee osteoarthritis (KOA) rabbits, and to explore the biomechanical effects of acupotomy on KOA.@*METHODS@#Twenty-four New Zealand male rabbits were randomly divided into four groups: normal group, model group, electroacupuncture group and needle-knife group, with 6 rabbits in each group. In each model group, rabbit KOA model was established by fixing Videman's left hind limb in straight position for 6 weeks. In the electroacupuncture group, rats were treated left on Liang Qiu, Xue Hai, Nei Xi Yan and Wai Xi Yan 3 times a week for 3 weeks. In the acupotomology group, the left quadriceps femoris tendon was released with acupotomology, and the treatment was once a week for 3 weeks. Behavioral tests were performed using Lequesne MG knee joint evaluation method one week after the end of modeling and one week after the end of treatment, and HE staining and mechanical tests were performed one week after the end of treatment.@*RESULTS@#Behavioral observation before treatment showed that there were significant differences in local pain, gait response, joint activity and joint swelling between the normal group and the model group(0.05). After treatment, the results showed that there were significant differences in local pain, gait response, joint activity and joint swelling among model group, electro-acupuncture group and needle-knife group compared with normal group(0.05); there was significant difference between needle knife group and model group(0.05). In gait change, there was significant difference between model group and electro-acupuncture group(0.05). In joint activity, there was significant difference between electro-acupuncture group and model group(0.05). Mechanics: Compared with the blank group, the ultimate load of the model group decreased significantly(0.05), and the ultimate load of the needle-knife group increased(>0.05). Compared with the model group, the ultimate load of the electro-acupuncture group increased significantly(0.05). Compared with the blank group, the maximum displacement of the model group decreased significantly(0.05). Compared with the model group, the maximum displacement of the electro-acupuncture group increased(>0.05), and the maximum displacement of the needle-knife group increased significantly(0.05). There was no significant difference in stiffness among groups(>0.05).@*CONCLUSIONS@#Acupotomy intervention can significantly change the behavior and morphology, significantly improve the mechanical properties of quadriceps femoris tendon stretch, and exert its biomechanical effects to achieve the purpose of treating KOA.
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Animals , Humans , Male , Rabbits , Rats , Electroacupuncture , Knee Joint , Osteoarthritis, Knee , Quadriceps Muscle , TendonsABSTRACT
This research is launched to look for the medication rules and characteristics of Tibetan medicine in the treatment of gZav-Grib( apoplexy sequelae). HIS records of gZav-Grib patients were selected from the Tibetan Hospital of Tibet Autonomous Region and Tibetan Hospital of the city of Naqu. SPSS Modeler,Gephi and other data mining and visualization software were used to study the actual law of drug use in the treatment of gZav-Grib in Tibetan medicine. Finally,479 cases of gZav-Grib patients in Tibetan medicine were included. Their average age is 63 and average hospital stay was 32 days. In total,82 Tibetan medicine prescriptions were used for treating gZav-Grib. The frequency in the front is Twenty-flavor Chenxiang Pills( 338 times),Ruyi Zhenbao Pills( 322 times),and Seventy-flavor Zhenzhu Pills( 315 times). According to the regularity of Tebitan medicine,they were applied in different time periods including the early morning,morning,noon,and evening,for example,in the early morning: Seventy-flavor Zhenzhu Pills,morning: Ruyi Zhenbao Pills,noon: Eighteen-flavor Dujuan Pills,evening: Twenty-flavor Chenxiang Pills. In the clinical joint,18 groups were found in the 10% support and 70% confidence. There are two prescriptions confidence more than 80% which nature focus on Gan,Ruan,Xi,Liang,Dun,Han,Wen. gZav-Grib of Tibetan medicine can be divided into two types: r Lung-Grib type and k Hrag-Grib type,in which the medicine of r Lung-Grib type takes Seventy-flavor Zhenzhu Pills as the core prescription,while the medicine of k Hrag-Grib type takes Ruyi Zhenbao Pills as the core prescription. It is found that the treatment of gZav-Grib by Tibetan medicine is mainly dominated by the treatment idea about " Therapeutic r Lung and blood,Consideration of venous diseases". Treatment functions is promoting the circulation of Qi,clearing blood heat and getting rid of bad blood,achieving the purpose of treating both principal secondary aspect of gZav-Grib. The research methods based on the HIS can't only optimize the Tibetan treating gZav-Grib sequela treatment plan and rule of medication,but also provide the scientific basis for Tibetan medicine treat gZav-Grib.
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Humans , Data Mining , Medical Records , Medicine, Tibetan Traditional , Software , Stroke , Drug Therapy , TibetABSTRACT
OBJECTIVE:To provide reference for the further research and development of bamboo juice. METHODS:Comb-ing the ancient and modern literatures,the processing historical evolution,origins,technology,chemical ingredients,quality stan-dards and efficacy were analyzed,the problems were found and solutions were put forward. RESULTS:Bamboo juice's processing can be traced back to the Liang Dynasty,still being used today with light bamboo juice. And now,varieties of bamboos(Phyllo-stachys glauca,Phyllostachys viridiglaucescens,Pleioblastus amarus,and Neosino calamus,etc.) were processed into medicine. The research for the chemical ingredients was mainly for the changes of bamboo juice with different varieties and amino acids,phe-nols,organic acids and inorganic elements in bamboo juice with different technologies,and there was no comprehensive study on the material basis of bamboo juice. Its quality standards must be improved. Although there was comparative study for the traditional processing technology and modern technology in chemical ingredients,the study did not compare the efficacy of its material founda-tions. The research for the efficacy of bamboo juice only involved relieving cough and resolving phlegm,and its relieving convul-sion,resuscitation,calming diabetes and other pharmacological effects haven't been reported. CONCLUSIONS:In the future, in-dept research can be carried out from the aspects of standardizing the name,base source and processing technology parameters, improving quality standards,strengthening basic research of effective substances,and improving traditional technology and equip-ment level.
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Objective:To study the effects of insulin and berberine on osseointegration of titanium implant in type Ⅱ diabetic rats.Methods:10 out of 53 SD rats were randomly selected and selected into H group(healthy group)(n =10).The remaining rats were treated with high glucose diet plus low dose of streptozotocin(STZ) and 40 rats were induced into the models of type Ⅱ diabeties.The 40 dibetic rats were randomly divided into group B(berberine),group Ⅰ(insulin),group IB(insulin + berberine) and group N(without treatment).2 pure titanium implants were implanted into the bilateral femoral metaphysis of each rat.Mter 7,32,38,66 and 101 d,the body weight(BW) and fasting blood glucose(GLU) were measured.After 10 week,the rats were sacrifice,femoral metaphysis samples were subjected to Micro-CT scan,VG staining and implant pull-out test.Hard tissue slices were observed;bone volume fraction(BV/TV),Liang Houdu (Tb.Th),trabecular bone trabecular number(Tb.N),Liang Jianxi (Tb.Sp),trabecular bone trabecular bone volume (TBV),bone contact ratio(BIC) and the maximum pull-out strength (MPF) were measured.Results:There was no significant difference of BW and GLU between IB group and H group(P ＞ 0.05),but the BW and GLU of IB group was better than those of Ⅰ group and B group,Ⅰ group and B group were better than those of group N (P ＜ 0.05).BV/TV,Tb.Th,Tb.N,Tb.Sp,TBV,BIC and MPF of IB group were not statistically different from those of H group(P ＞0.05);but the indexes of IB group were higher than those of Ⅰ group and B group,those of Ⅰ group and B group were higher than those of N group (P ＜ 0.05).Conclusion:The combination of insulin and berberine can effectively reduce blood glucose and can promote osseointegration of titanium implants in type Ⅱ diabetic rats.
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OBJECTIVE:To provide reference for the further research and development of bamboo juice. METHODS:Comb-ing the ancient and modern literatures,the processing historical evolution,origins,technology,chemical ingredients,quality stan-dards and efficacy were analyzed,the problems were found and solutions were put forward. RESULTS:Bamboo juice's processing can be traced back to the Liang Dynasty,still being used today with light bamboo juice. And now,varieties of bamboos(Phyllo-stachys glauca,Phyllostachys viridiglaucescens,Pleioblastus amarus,and Neosino calamus,etc.) were processed into medicine. The research for the chemical ingredients was mainly for the changes of bamboo juice with different varieties and amino acids,phe-nols,organic acids and inorganic elements in bamboo juice with different technologies,and there was no comprehensive study on the material basis of bamboo juice. Its quality standards must be improved. Although there was comparative study for the traditional processing technology and modern technology in chemical ingredients,the study did not compare the efficacy of its material founda-tions. The research for the efficacy of bamboo juice only involved relieving cough and resolving phlegm,and its relieving convul-sion,resuscitation,calming diabetes and other pharmacological effects haven't been reported. CONCLUSIONS:In the future, in-dept research can be carried out from the aspects of standardizing the name,base source and processing technology parameters, improving quality standards,strengthening basic research of effective substances,and improving traditional technology and equip-ment level.
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Objective To learn the burnout and turnover intention of physicians from township hospitals in Xi County,Henan province,for reference of health administrators and healthcare institution managers.Methods Cluster sampling method and qualitative interviews were used and all physicians from such hospitals were sampled for questionnaire survey in 2014,with 1 70 scales distributed.The questionnaire was the Markov Burnout Inventory generic version revised by Li Chaoping,and turnover intention questionnaire was that revised by Liang Kaiguang.Results 140 physicians from such hospitals were found with zero or low burnout (94.6%);Collaborative care in the county poses great positive effect on promoting professionalism and interpersonal relations, yet has limited positive impact on reducing workload and performance appraisal stress and increasing their income.Conclusion It is imperative to enhance training of managers of such hospitals for them to learn the appraisal method with organizational scale,and build a humanistic concern mechanism for physicians,for purposes of improving the organizational environment and minimize the burnout and turnover intention of the physicians.
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Objective:To observe the effect of complex reinforcing [Shao Shan Huo (Mountain-burning Fire)] and reducing [Tou Tian Liang (Heaven-penetrating Cooling)] manipulations on motion and balance of the lower limbs in patients with subacute combined degeneration of the spinal cord (SCD). Methods:A total of 100 SCD cases were randomly allocated into an observation group and a control group by their visit sequence, 50 cases in each group. On the basis of Western medical treatment, cases in the observation group were also treated with acupuncture therapy plus complex reinforcing-reducing manipulation; whereas cases in the control group were only treated with the same Western medical treatment as those in the observation group. Before and 2 months after treatment, kinematic parameters including muscle force, muscle tone and range of motion of the hip and knee joints were measured. In addition, balancing parameters including the path length of center of pressure (COP), peripheral area, COP path length per unit area and rectangular area were also measured before and after treatment. Results:After treatment, there were statistical intra-group differences in COP path length, peripheral area, COP path length per unit area, left-right offset, rectangular area, coefficient of stability and coefficient of weight distribution (P<0.01). There were statistical inter-group differences in muscle force, muscle tone and range of motion of hip and knee joints (P<0.01). The total effective rate was 83.3% in the observation group, versus 60.0% in the control group, showing a statistical difference (P<0.05). Conclusion:Complex reinforcing-reducing manipulations can improve the lower limb motion and balance in SCD patients.
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This study was aimed to prove the scientific method theory through revealing the effect of synchronous treatment of lung and intestine in the treatment of active ulcerative colitis (AUC) patients. A total of 90 cases with traditional Chinese medicine (TCM) diagnosis of heat-toxin syndrome and western medical diagnosis of AUC were randomly divided into the control group, treatment of intestine group, and synchronous treatment of lung and intestine group, with 30 cases in each group. SASP enema (control group), SASP with Bai-Tou-Weng (BTW) decoction and Tiao-Wei Cheng-Qi (TWCQ) decoction enema (treatment of intestine group), SASP with BTW decoction and Liang-Ge-San (LGS) enema (synchronous treatment of lung and intestine group) were given, respectively. The treatment was given once a day and continued for 60 days. Then, clinical therapeutic evaluation was given before and after the treatment with enteroscopy, Th1/Th2 of ulcerative colon tissues was detected. The results showed that the sequence of clinical effect from top to bottom was synchronous treatment of lung and intestine group, treatment of intestine group, control group, with statistical significance (P< 0.05 or P< 0.01). Comparison of Th1/Th2 before and after treatment among each group showed that Th1 (INF-γ, IL-2) was decreased, Th2 (IL-4, IL-5) was increased (P< 0.05), Th1/Th2 was decreased, with statistical significance (P< 0.01). Comparison among each group after treatment showed that the sequence of Th1 from top to bottom was control group, treatment of intestine group, synchronous treatment of lung and intestine group; that of Th2 was synchronous treatment of lung and intestine group, treatment of intestine group, control group; that of Th1/Th2 was control group, treatment of intestine group, synchronous treatment of lung and in-testine group, with statistical significance (P< 0.01). It was concluded that synchronous treatment of lung and intes-tine had guiding significance on anti-inflammatory effect of enema therapy to AUC.
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Objective To study the effects of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis.Methods Sixty male SD rats were divided into three groups randomly:normal rats as control,abdominal radiation control group given distilled water after 10 Gy radiation,and abdominal radiation plus drug group given Liang-Xue-Yu-Chang decoction after 10 Gy radiation.Drugs or distilled water were chronically administered to animals for 7 days.After that,5-bromodexyridine (BrdU) was injected into abdominal cavity,SP immunohistochemistry was used to evaluate intestinal epithelium proliferating cell nuclear antigen (PCNA) and the positive cells labeled with Ki67 and BrdU.Results The number of intestinal epithelium cells with PCNA expression,positive Ki67 and BrdU were 22.0 + 2.7,71.2 + 5.7,and 26.2 + 5.9 respectively in the drug treatment group,significantly higher than those in the abdominal radiation control group (11.2 + 1.9,61.6 ± 5.5,and 11.3 ± 2.2) (t =14.629,5.420,11.582,P ＜ 0.05),but lower than those in the normal control group (30.4±5.7,86.6 ±5.1,and 32.3 ±3.2)(t =14.291,9.004,4.731,P＜0.05).Conclusion Liang-Xue-Yu-Chang decoction could improve the proliferation of intestinal epithelium in the rats with radiation enteritis.
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<p><b>OBJECTIVE</b>To study the effects of "XUE BI JING plus LIANQIAO" injection on gene expression levels of rats with sepsis model.</p><p><b>METHODS</b>One hundred and twenty rats were randomly divided into sham operation group, sepsis model group, Te-neng group and "XUE BI JING plus forsythia suspension" group. The sepsis model of rats was prepared by "CLP" method. Tai neng group was treated by peritoneal injection Imipenem/ Cilastatin (0.18 g/kg); "XUE BI JING plus LIANQIAO" group was treated by peritoneal injection Imipenem/ Cilastatin (0.18 g/kg) plus "xue-bi-jing" (10 ml/kg) and "liang ge san" (18 g/200 g) by intragastric administration 2 times a day; the sham operation group and model group were treated by peritoneal injection of normal saline (10 ml/kg). The survival rates at 48h and 72h were observed for all groups. The gene expression levels of livers in all groups were detected by BiostarR-40s chip. The NCBI database was used to inquest Gene function and class.</p><p><b>RESULTS</b>The survival rates at 48h and 72h in "XUE BI JING+ forsythia suspension" group were 83.3% and 76.7% which were significantly higher than those (30.0% and 16.7%) in sepsis model group and those (60.0% and 33.3%) in Te-neng group (P < 0.01). Model group/control group have 305 differential expression genes with 159 up-regulation genes and 146 down-regulation genes. Tai-neng group/model group have 386 differential expression genes with 206 up-regulation genes and 180 down-regulation genes. "XUE BI JING plus forsythia suspension" group/model group have 342 differential expression genes with 102 up-regulation genes and 240 downregulation genes. The genes with up-regulation in model group/ control group and with down-regulation in"XUE BI JING plus forsythia suspension" group/model group were 24. The genes with down-regulation in the model group/ sham operation group and with up-regulation in "XUE BI JING plus forsythia suspension"group/model group were 16.</p><p><b>CONCLUSION</b>"XUE BI JING plus forsythia suspension" can reduce the mortality of rats with sepsis, which could be due to the expression of relative regulation genes.</p>
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Animals , Male , Rats , Disease Models, Animal , Drugs, Chinese Herbal , Pharmacology , Forsythia , Gene Expression , Gene Expression Regulation , Liver , Metabolism , Oligonucleotide Array Sequence Analysis , Phytotherapy , Rats, Wistar , Sepsis , Drug Therapy , Genetics , MetabolismABSTRACT
A dietary survey was conducted with the 3-day dietary intake record in 167 type 2 diabetic patients in Nandan Community of Xujiahui Subdistric of Shanghai, the results were transferred to healthy eating index (HEI). The average score of HEI was 74. 0. According to the criteria of HEI, diet quality of 7. 8% patients was "bad", that of 10. 8% patients was "good" and that of 81.4% patients was "need improvement". In all components, the fruits consumption was the worst, especially for male patients.Patients with higher educational levels had better dietary quality and patients who were on working had better HEI score than who retired. The obese and overweight patients consume more grains, meat and total fat than those with normal body weight.
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BACKGROUND: At present, it is affirmed that coping style is the important mediator between stress and health both on mental and body. The coping theory is descripted as three orientations: trait view, contextual view and interaction person-environment view. The conception and classification of the coping styles still remains to be explored. OBJECTIVE: To probe into the dimensions of coping styles, the characteristics of different coping styles and the correlation between coping styles and psychological adaptation among college students.DESTGN: Questionnaire investigation.SETTING: School of Psychology, Beijing Normal University.PARTICIPANTS: Altogether 343 college students were randomly chosen from Tianjin University, Tianjin University of Commerce, Tianjin Normal University and Tianjin University of Finance and Economics. The college students, including 161 males and 182 females, were aged (19±2) years, ranging from 19 to 22 years. According to body health examniation records of them, all the college students had no serious chronic or acute illness. Written informed consents were obtained from all the college students.METHODS: The college students were measured collectively in their own classroom respectively from September to December in 1999. There were totally 16 class units. Altogether 422 copies of questionnaire files were sent out. Each copy includes Coping Styles Scales, Adolescent Psychological Adaptation Scales, and SCL-90.The questionnaires were collected once the college students completed. Copying styles were assessed with Coping Style Scale revised by Liang Baoyong, which includes 20 items. Each item gives one kind of coping strategies in daily life. For example, "To tell myself,my trouble is nothing matter When comparing with others, it is no worthy to be annoyant". The college students should be asked to answer on the 5-point Likert scale. These 20 items covered majority of coping styles which were used by adolescents. Two indexes were used as the results of psychological adaptation: one is Adolescent Psychological Adaptation Scale developed by Chen Huichang, including 20 items. Scores were collected on the 5-point Likert scale in which high scores mean better adaptation. Another result variable is the amount of SCL-90. High scores mean more symptoms with mental or physical state which stands for lower adaptation level. All the assessment data were input into Foxbase data-base, then were analyzed using factor analysis and cluster analysis in order to attain different kinds of college students coping styles. Correlation analysis was used to examine the correlation between coping style and adaptation. MAIN OUTCOME MEASURES: Coping styles of college students and correlation between different coping styles and adaptation results.RESULTS: A total of 422 copies of questionnaires were sent out, and 362 copies were drawn back, 19 copies were invalid questionnaires due to either there were missing answers or the answer had reflective tendency. At last, 343 sets of data entered the analysis procedure. ① According to factor analysis results, the main coping styles used by college students were F1 (seeking support), F2(positive thinking), F3(cognitive adjustment), F4(passive blame oneself), F5(facing reality), F6(avoidance), F7(emotional catharsis).There were three main coping styles from cluster analysis, that was, A (positive confrontation), B (passive avoidance) and C(passive catharsis).② Those who were more adaptive used A coping style more frequently, and those who had lower adaptation level used B or C coping style more frequently. But few students used C coping style. A coping style was significantly negatively correlated with mental or physical symptoms (r= -0.258,P＜ 0.01 ),but significantly positively correlated with adaptation level (r =0.467, P ＜ 0.01);But both B and C coping styles were significantly positively correlated with mental or physical symptoms (r=0.338, 0.364, P ＜ 0.01 ), B coping style was significantly negatively correlated with psychological adaptation(r =-0.140, P ＜ 0.05). It seemed that C coping style had no correlation with long-term adaptation. A and C coping styles were two dependent styles respectively. In addition, different levels of stress were significantly correlated with three styles. Few A coping styles were used under high level of stress, but more B and C coping styles did.CONCLUSION: Coping style is complex. Use of different copying styles is significantly correlated with psychological adaptation levels. But the direction of correlation is effected by the factors, such as individuals, index of results and degree of stress.
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Objective To explore the changes on autonomic nervous function of the patients with different Traditional Chinese medicine syndrome types.Methods The cases of anxiety or depressive diseases were divided into different types according to traditional Chinese medicine syndrome types,and then heart rate variability(HRV) were tested and compared with normal controls.Results Indicators of time or frequency of heart rate variability(HRV) in anxiety or depressive disease patients with Gan-Yu-Tan-Zu Zheng or Xin-Pi-Liang-Xu Zheng or Gan-Yu-Qi-Zhi Zheng were lower than those of controls group,especially in parasympathetic nervous.The abever disorders of the patients with Gan-Yu-Tan-Zu Zheng or Gan-Yu-Qi-Zhi Zheng was more significant than those in patients with Xin-Pi-Liang-Xu Zheng.Conclusions The autonomic nervous function of anxiety or depressive diseases with Gan-Yu-Tan-Zu Zheng or Xin-Pi-Liang-Xu Zheng or Gan-Yu-Qi-Zhi Zheng is disordered and their HRV of was lower than that of control(P
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Objective To compare the differences and consistency of three criteria of metabolic syndrome(MS)including the third Report of the Adult Treatment Panel Ⅲ of National Cholesterol Education Program(NCEP-ATPⅢ),Chinese Diabetes Society(CDS)and International Diabetes Federation(IDF)used for evaluating in children and adolescents.Methods Four hundred and thirty-two overweight or obesity children and adolescents from Chuansha district of Pudong New Area of Shanghai were selected.Data of investigation of the risk factors of cardiovascular disease were reviewed.Anthropometric measurements and fast plasma glucose(FPG),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C)and dynamic blood pressure monitoring were performed.MS were identified according to NCEP-ATPⅢ criteria modified by Cook,CDS criteria modified by Liang,and IDF criteria,respectively.The consistency of the 3 criteria were studied.Results The number of subjects who met the criteria of MS were 31.0%,9.5% and 25.7% using Cook,Liang and IDF criteria,respectively.The conformity rate between Cook and Liang,Cook and IDF,Liang and IDF were 78.5%,89.1% and 83.8%,and the Kappa value were 0.378 20,0.719 18 and 0.465 37,respectively.Conclusions There are differences in consistency among the 3 criteria of MS.The agreement in the diagnosis of MS using Cook and IDF definition is higher than using Liang and other 2 definitions.The number of subjects who met the Cook criteria of MS is the most.The Cook definition may be more suitable for MS diagnosis in children and adolescents.A high conformity rate between Cook and and IDF criterion is proved.It is necessary to work out a universally accepted criterion adapted to children and adolescents.