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Objective: To isolate and identify the chemical constituents from the rhizomes of Polygonatum sibiricum. Methods: The compounds were isolated and purified by various modern chromatographies, and their structures were identified by physiochemical properties and spectroscopic data. Results: Five compounds were isolated from the EtOH extract of the rhizomes of P. sibiricum, which were elucidated as polygonneolignanoside A (1), (+)-isolariciresinol-9'-O-β-D-glucopyranoside (2), trans-N-p- coumaroyltramine (3), 3-(4-hydroxyphenyl)-N-[2-(4-hydroxyphenyl)-2-methoxyethyl]acrylamide (4), 3-(4-hydroxy-3-methoxyphenyl)- N-[2-(4-hydroxyphenyl)-2-methoxyethyl]acrylamide (5). Conclusion: Among them, compound 1 was a new benzofuran lignan, and compounds 2-5 were isolated from this plant for the first time.
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Objective To investigate the chemical constituents of Cynanchum auriculatum. Methods Compounds were isolated and purified by using a combination of various chromatographic techniques including D-101 macroporous adsorptive resins, silica gel, Sephadex LH-20, and other methods. Their structures were elucidated by MS, NMR, and other modern spectroscopies. Results Fifteen compounds were isolated and identified as isocopoletin (1), isofraxidin (2), deacetylmetaplexigenin (3), vomifoliol (4), 4,4-dimethyl heptanedioic acid (5), (+)-isolariciresinol (6), 3-hydroxypyridine (7), 3-hydroxy-2-methylpyridine (8), 5-hydroxyl-2-hydroxymethylpyridine (9), 2-methyl-6-(2’,3’,4’-trihydroxybutyl)-pyrazine (10), kiwiionol (11), picein (12), adenosine (13), cynanoneside B (14), and cynanoneside A (15). Conclusion Compounds 1-2, 4-13 are isolated from the plants of genus Cynanchum Linn for the first time, while compound 15 is firstly obtained from C. auriculatum.
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Objective To study the chemical constituents from the seeds of Lepidium apetalum. Methods Compounds were isolated from the water extract from the seeds of L. apetalum by using Diaion HP-20, Toyopearl HW-40, MCI Gel CHP-20, ODS, Silica gel chromatography and semi-preparative-HPLC. Results Nine compounds were isolated and identified as lepidiumlignan A (1), lepidiumlignan B (2), erythro-1-(4-O-β-D-glucopyranosyl-3-methoxyphenyl)-2-[4-(3-hydroxypropyl)-2,6-dimethoxyphenoxy]-1,3- propanediol (3), (7R,7’E,8S)-4,9-dihydroxy-3,3’,5-trimethoxy-4’,7-epoxy-8,5’-neolign-7’-en-9’-oic acid (4), spicatolignan B (5), (-)-pinoresinol-4-O-β-D-glucopyranoside (6), (-)-isolariciresinol (7), aegineoside (8), and (+)-syringaresinol-O-β-D-glucopyra- noside (9). Conclusion Compounds 1 and 2 are new compounds, named as lepidiumlignan A and lepidiumlignan B. Compounds 3-9 are isolated from the plants for the first time.
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Objective To investigate the hydrophilic constituents from the anti-colorectal cancer extract of Oplopanax elatus. Methods The compounds were isolated and purified using macroporous resin, silica gel, ODS gel and pre-HPLC, and their chemical structures were identified by spectral data and physicochemical properties. The extracts and compounds from O. elatus were screened for anti-proliferation on HCT-116 and HT-29 cancer cell lines. Results Eleven phenolic compounds had been purified and identified from the n-butanol fraction including six phenylpropanoid glycosides: (E)-sinapic acid-4-O-β-D-glucopyranoside (1), 3- hydroxyphenethyl alcohol-4-O-β-D-glucopyranoside (2), 3-methoxycinnamyl alcohol-4-O-β-D-glucopyranoside (3), homovanillyl alcohol-4-O-β-D-glucopyranoside (4), dihydrosyringin (5), and syringin (6); And five lignan glycosides: 3,3'-dimethoxy-4,9,9'- trihydroxy-4',7-epoxy-5',8-lignan-4,9-bis-O-β-D-glucopyranoside (7), (+)-5,5'-dimethoxylariciresinol 4'-O-β-D-glucopyranoside (8), (+)-isolariciresinol-9'-O-β-D-glucopyranoside (9), (+)-isolariciresinol-4-O-β-D-glucopyranoside (10), and (+)-5,5'-dimethoxylariciresinol- 9'-O-β-D-glucopyranoside (11). All the phenolic glycosides showed no significant effects on the proliferation of HCT-116 and HT-29 cancer cell lines with IC50 > 100 μmol/L. Conclusion Compounds 4, 6, 9, and 11 are isolated and purified from this herb for the first time, while compounds 1, 2, and 10 are firstly obtained from the genus Oplopanax.
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OBJECTIVE: To investigate the cytotoxic activities of chemical constituents in alcohol extract of the stem bark of Murraya exotica L. METHODS: The cytotoxicity against five cancer cell lines, U937, HL-60, K562, Bel7402 and Hela, were assayed by MTT and SRB METHODS. The constituents were isolated from Murraya exotica L. by routine chromatographic METHODS and the structures of the isolates were elucidated by NMR techniques. The antitumor effect was evaluated on cancer cells in vitro. RESULTS: When the cancer cells were exposed to the extract for more than 48 h, the survival rate decreased with the increase of the drug concentration. Four compounds were isolated and identified as isolariciresinol (I), dimethoxy isolariciresinol (II), 3-methoxyisolariciresinol (III), and 5'-methoxyisolariciresinol (IV). CONCLUSION: The active ingredients in Murraya exotica L. have antitumor activities, which may be compounds I and II.
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Objective: To study the chemical constituents in the whole plants of Saururus chinensis. Methods: The chemical constituents were isolated by various column chromatographic methods. The structures of the compounds were elucidated on the basis of physiochemical properties and spectrascopic analysis. Results: Seven compounds were obtained and identified as trans-7, 8-dihydro-7-(3,4-methylenedioxyl)-phenyl-1'-(2-oxopropyl)-3'-methoxy-8-methylbenzofuran (1), 4-(3-methoxy-4-hydroxy) phenyl-3- methyl-3-buten-2-one (2), (+)-guaiacin (3), (+)-trans-1,2-dihydrodehydroguaiaretic acid (4), (-)-isolariciresinol-4-O-β-D-glucoside (5), isonectandrin B (6), and perseal F (7), respectively. Conclusion: Compound 1 is a new compound named as saurusine B, and compounds 2-7 are obtained from this plant for the first time.
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Objective: To study the chemical constituents from the roots of Kadsura longipedunculata. Methods: The constituents were isolated and purified by various chromatographic methods, and the structures were elucidated by spectroscopic analysis. Results: Sixteen compounds were isolated from the roots of K. longipedunculata and the structures were identified as pinobatol (1), leptolepisol B (2), 7S,8R-erythro-4,7,9,9'-tetrahydroxy-3,3'-dimethoxy-8-O-4'-neolignan (3), 2,3-bis-(α-hydroxy-4-hydroxy-3-methoxybenzyl)-butane-1,4-diol (4), (7'S,8R,8'S)-4,4',9-trihydroxy-3,3',5-trimethoxy-9'-O-β-D-xylopyranosyl-2,7'-cyclo-lignan (5), aviculin (6), ent-isolariciresinol (7), lawsorosemarinol (8), (+)-anwulignan (9), isolariciresinol-2α-O-β-D-xyloside (10), procyanidin B3 (11), prodelphinidin B3 (12), (-)-gallocatechin (13), (+)-catechin (14), abscisic acid-β-D-glucopyranosyl ester (15), and (-)-oleuropeic acid 8-O-β-D-glucopyranoside (16). Conclusion: Compounds 1-8, 11-13, 15, and 16 are isolated from the plants of Kadsura Kaempf. ex Juss. for the first time.
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Objective: To study the chemical constituents from the whole plant of Goldfussia pentstemonoides. Methods: The compounds were separated and purified by chromatographic methods. The structures were identified by spectroscopic analyses. Results: Ten compounds were isolated from the n-butanol fraction of 95% ethanol extract in the whole plants of G. pentstemonoides and identified as salidroside (1), 1-(α-L-rhamnosyl-(1→6)-O-β-D-glucopyranosyloxy)-3,4,5-trimethoxybenzene (2), isoacteoside (3), isonuomioside A (4), benzyl-O-β-D-glucopyranoside (5), lcariside F2 (6), (-)-lyoniresinol 3α-O-β-D-glucopyranoside (7), (+)-isolariciresinol-9-O-β-D-glucopyranoside (8), decaffeoylverbascoside (9), and dehydrodiconiferyl alcohol 4-O-β-D-glucopyranoside (10). Conclusion: All compounds are isolated from G. pentstemonoides for the first time.
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OBJECTIVE: To study the chemical constituents of the roots of Rhodiola crenulata. METHODS: The roots of R. crenulata were extracted with 80% ethanol, and the extract was partitioned with ethyl acetate. The chemical components were isolated by various chromatographic methods. Their structures were elucidated by spectral analysis. RESULTS: Eleven compounds were isolated and identified as isolariciresinol-9, 9′-acetonide (1), p-coumaric acid (2), p-coumaric acid-4-O-β-D-glucopyranoside (3), tachio-side (4), dihydroconiferin (5), 2-phenylethyl β-glucopyranoside (6), 2-phenylethyl β-vicianoside (7), 2-phenylethyl-6-0-α -L-arabi-nofuranosyl-β-D-glucopyranoside(8), 2-phenylethyl β-primeveroside (9), benzyl β-D-glucopyranoside (10), and rhodiocyanoside D (11). CONCLUSION: All of the compounds were isolated from Rhodiola crenulata for the first time, and compounds 1, 3, 4, 5, 7, 9 were obtained from the genus of Rhodiola for the first time.
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Objective: To investigate the chemical constituents from the twigs of Myricaria bracteata. Methods: The chemical constituents were isolated and purified by column chromatography. Their structures were identified on the basis of spectroscopic data, such as NMR, MS, and physicochemical properties. Results: Fourteen compounds were isolated from M. bracteata and identified as syringaresinol (1), (-)-lyoniresinol (2), (-)-isolariciresinol (3), N-trans-feruloyltyramine (4), N-trans-feruloyl-3-methoxytyramine (5), N-trans-feruloyl-2'-methoxytyramine (6), kaempferol-3-O-β-D-glucuronic acid methylester (7), quercetin-3-O-β-D-glucuronic acid methylester (8), quercitrin (9), rhamnocitrin (10), gallic acid (11), gallicin (12), E-caffeic acid (13), and E-ferulic acid (14). Conclusion: Compounds 1-6 and 14 are isolated from the plants of this genus for the first time. Compounds 7, 8, 12, and 13 are isolated from the twigs of M. bracteata for the first time.