Study on the islet ? cell function and insulin resistance of prediabetic population / 中国糖尿病杂志

Objective To analyze the islet ? cell function and the insulin resistance of different components of impaired glucose regulation(IGR).Methods A total of 463 adults diagnosed as IGR and 200 adults diagnosed as NGT by 75g OGTT,were included for the analysis of the islet beta cell function and insulin resistance.Results In the IFG group,HOMA-IR was significantly higher than in NGT and IGT group(P

Effect of Various Kind of Dyslipidaemia on Insulin Resistance and Pancreatic Islet Function in Newly Diagnosed Type 2 Diabetic Patients / 中国医师杂志

Objective To evaluate the effect of various kinds of dyslipidaemia on insulin resistance and pancreatic islet ? cell function in newly diagnosed type 2 diabetic patients. Methods 263 patients with newly diagnosed type 2 diabetes were divided into normal lipidemia group and dyslipidaemia group according to ATPⅢ standard. The dyslipidaemia group included 3 subgroups as the following: high TG group, high TC group, and mix group with high TG and TC. Homeostasis model assessment (HOMA) was applied to estimate insulin resistance (IR) and pancreatic islet ? cell function. Early insulin secretion index (EISI) and area under curve of insulin (AUCI) at all OGTT time points were calculated. Results Incidence of dyslipidaemia in the type 2 diabetic patients was 63%. BMI and WHR were significantly higher in both high TG group and mix group than in normal lipidemia group(P

Changes of islet ?-cell function in three groups of normal glucose tolerance (NGT), impaired glucose tolerance(IGT) and type 2 diabetes(DM) / 中国糖尿病杂志

0.05). Other indexes value are as follows: IR 1.1?0.3(NGT),1.3?0.6 (IGT) and 1.6?0.5 (DM), P

Study of the relationship between fasting blood glucose and islet ? cell function / 中国糖尿病杂志

411 subjects were divided into six groups according to their FPG levels.And OGTT was carried out.Result showed that along with the FPG increasing, HOMA ?-cell index and ?I30 /?G30 were decreased progressively, and the fasting serum proinsulin level and ratio of proinsulin /insulin were increased markedly.The area under insulin curve decreased gradually when FPG was above 8 mmol/L. In conclusion, FPG is a reflection of islet ? cell function.

Effect of high glucose toxicity on JNK pathway,cell viability and apoptosis in pancreatic ?-cell line INS-1 / 中国病理生理杂志

AIM:To investigate the effect of high glucose toxicity on JNK pathway and cell function of INS-1 cells.METHODS: Cultured INS-1 cells with or without IGF-1 exposure,were treated with glucose at 3 concentrations (5.6 mmol/L,11.2 mmol/L and 33.3mmol/L),respectively. MTT was used to measure the cell viability. Apoptosis was determined by immuno-fluorescence and flow-cytometry analysis. The serine 270 phosphorylation of IRS and phosphorylation of JNK in INS-1 cells were detected in the presence or absence of SP600125 treatment.RESULTS: The cell viability decreased and apoptosis increased with elevated glucose concentrations. The percentage of apoptosis cells was 11.3% in 5.6 G group,12.7% in 11.2 G group and 28.2% in 33.3 G group. There was remarkable increase in apoptosis in 33.3 G group with a 2.49-fold increase to the cells in the basal 5.6 mmol/L glucose. High glucose activated the serine 270 phosphorylation of IRS correlates with JNK phosphorylation in INS-1 cells. Using Western blotting analysis,the levels of JNK phosphorylation were 3.33 fold increased and serine 270 phosphorylation of IRS was 1.17 fold increased in 33.3 G group compared to 11.2 G group (P