ABSTRACT
Objective To investigate the effects of radix salviae miltiorrhiaze(RSM) on the expression of Fas, perforin and bcl 2 mRNA in liver ischemia reperfusion(I R). Methods 45 patients were divided into three groups: Control group(group 1, n=5); I R group(group 2, n=20), and RSM pretreatment group(group 3, n=20). The patients in group 2 and group 3 subjected to hepatic resection with “Pringle procedure” for 15?min. Expression of Fas, perforin and bcl 2 mRNA were examined with reverse transcription polymerase chain reaction(RT PCR) after liver reperfusion for 40?min. Results Expression of Fas, perforin were very weak in control group(0.144?0.037; 0.106?0.032), but increased in group 2(0.928?0.135; 1.099?0.364); and in group 3 was significantly lower(0.347?0.081; 0.454?0.116)than that in group 2(P
ABSTRACT
Objective To study the effect of hepatocyte growth factor (HGF) on hepatocytes after partial hepatectomy in chronic liver injury rats.Methods 30% hepatectomy was performed in rats with cirrhosis induced by CCL 4 and alcohol,and subcutaneously injection of cow HGF into intraabdomen was done with different dosages.Results Small dose (0.16mg?100g -1d -1)of HGF did not stimulate liver regeneration or enhance liver function after partial hepatectomy in cirrhotic rats.Large dose (1.3mg?100g -1d -1) of HGF stimulated liver regeneration and enhanced liver function after partial hepatectomy in cirrhotic rats(P