ABSTRACT
Chemical investigation of the dichloromethane extracts of Hoya multiflora Blume led to the isolation of lupeol (1a), α-amyrin (1b), β-amyrin (1c), lupeol acetate (2a), α-amyrin acetate (2b), and β-amyrin acetate (2c) from the stems; and 1b, bauerenol (3), squalene (4), lutein (5), β-sitosterol (6a), and stigmasterol (6b)from the leaves. The structures of 1-6 were identified by comparison of their 1H and/or13C NMR data with those reported in the literature.
ABSTRACT
Objective: The aim of the study was to investigate the prosexual activity of two compounds isolated from the hexane extract of the dried roots of Mondia whitei in rats. Methods: β-sitosterol and the mixture of α and β-amyrin acetate were isolated and characterised using several reagents, chromatography, mass spectrometry and physical analysis (RMN, 1H and 13C). Sexually inexperienced adult male rats were orally treated with these compounds at doses of 0mg/kg (control), 10mg/kg or 50mg/kg. 1h after the treatment, mount and intromission latencies and frequencies, penile erection, ejaculation latency and post-ejaculatory interval were measured for 60 minutes. Results: β-sitosterol and the mixture of α and β-amyrin acetate significantly increased the mount frequency (p0.05) when compared to control. The two purified compounds were efficient at the lower dose (10 mg/kg b.w) with β-sitosterol being the most potent. Conclusion:Results of the present work give added value to the aphrodisiac property of Mondia whitei and further justify its popular use as a sex stimulant.