ABSTRACT
Aims: There is little evidence concerning the effects of organophosphates in the liver of healthy individuals, and the existing researches come to contradictive results. In this study, we evaluated the influence of organophosphates (Dimethoate, Chlorpyrifos) in liver and renal function of healthy exposed workers, not experiencing symptoms of serious intoxication Study Design: Measure serum activity of the liver function monitoring enzymes SGPT, SGOT, γ-GT and ALP and serum concentration of the renal function indicative biomarkers urea and creatinine. Place and Duration of Study: Sample were collected in Health Care Greece of Iraklia Serres and analyzed in Department of Medical Laboratory Studies Alexander Technological Educational Institute of Thessaloniki. Methodology: Blood samples were collected from 112 individuals, randomly selected from villagers of N. Greece. 42 of them were organophosphates (OP) applicators aged less than 50 years old (mean age 37 years old) and 42 were OP applicators older than 50 years old (mean age 58 years old); while 28 individuals (13 of them were less than 50 years old and 15 older than 50 years) were not OP applicators and used as control groups. Results: A remarkable and statistically significant increase (P < 0.05) in the main liverfunction monitoring enzymes (SGOT, SGPT, γ-GT) was observed in exposed people compared to the control group. Increase in ALP values compared to not exposed individuals was not observed. Concerning the kidneys, data analysis shows that there is not any significant effect on their operation by the use of OP. Conclusion: The age of OP applicators and the time past between the application and the measure of blood serum seems to play an important role in the values of hepatic enzymes. While the renal indicators seemed not so much affected, as organophosphates are rapidly metabolized in human organism.
ABSTRACT
This study was carried out to evaluate the structure activity relationship and biochemical effects of some novel of anti-inflammatory triazoloquinazolines and triazinoquinazolines containing sulfacetamide moiety. Some of the newly synthesized compounds 4-17 showed good anti-inflammatory activities. Also, the median lethal doses (LD50s) of compounds 8, 10 and 17 in mice were 370, 338 and 295 mg/100g b.w., respectively. Oral administration of compounds 8, 10 and 17 to the rats at dose of 300 mg/kg.b.w. for 10 days showed non-significant changes in liver enzymes SGOT, SGPT, ALP, LDH, γ –GT, SOD and GPx and blood GSH and serum TBARs as compared with the control group. But, administration of indomethacin orally to the rats at a concentration of 600 mg/kg b.w daily for 10 days to rats showed significant increase in serum SGOT, SGPT, ALP, LDH, γ –GT and TBARs and significant decrease in blood GSH, SOD and GPx. These findings suggest that compounds 8, 10 and 17 exhibited good anti-inflammatory activity and more safe on rat liver enzymes.
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Objective: To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods: We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP), crag algae glycosidase (AFU), armor embryo protein heteroplasmon (AFPL3) and γ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. Results: Finally among the 30 patients in the early liver cancer group, 23 were positive with AFP, 20 with AFU, 15 with AFPL3 and 21 with γ-GT. Five were found positive with blood serum AFP, AFPL3, AFU and γ-GT at the same time; 5 with AFP, AFPL3 and γ-GT, 5 with AFP, AFU and AFPL3; 7 with AFP, AFU and γ-GT. By contrast, in the control group, among the 30 hepatitis liver cirrhosis patients and those chronic hepatitis B patients with liver cancer family history, 11 were found positive with AFP, 3 with AFPL3, 12 with AFU and 14 with γ-GT. None of the patients were found positive with union examination of AFP, AFPL3, AFU and γ-GT in the blood serum at the same time. Conclusion: The union examination of AFP, AFU, AFPL3 and γ-GT is significant to the early diagnosis of primary liver cancer.
ABSTRACT
Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP), crag algae glycosidase (AFU), armor embryo protein heteroplasmon (AFPL3) andγ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. Results Finally among the 30 patients in the early liver cancer group, 23 were positive with AFP, 20 with AFU, 15 with AFPL3 and 21 with γ-GT. Five were found positive with blood serum AFP, AFPL3, AFU and γ-GT at the same time; 5 with AFP, AFPL3 and γ-GT; 5 with AFP, AFU and AFPL3; 7 with AFP, AFU andγ-GT. By contrast, in the control group, among the 30 hepatitis liver cirrhosis patients and those chronic hepatitis B patients with liver cancer family history, 11 were found positive with AFP, 3 with AFPL3, 12 with AFU and 14 with γ-GT. None of the patients were found positive with union examination of AFP, AFPL3, AFU and γ-GT in the blood serum at the same time. Conclusion The union examination of AFP, AFU, AFPL3 and γ-GT is significant to the early diagnosis of primary liver cancer.
ABSTRACT
AimTo study the effect of the total extract of astragalosides(TEA) on the growth, AFP secretion and γ-GT activity of human hepatoma cells invitro.Methods The human hepatoma HepG2 and Bel-7404 cells were cultured with TEA 5～160 mg · L-1 for 6 days. Anti-hepatoma activity was measured by MTT method. AFP was assaysed by ELISA and γ-GT was determined by enzyme-substrate method. Results The growth of the human hepatoma HepG2 and Bel-7404 cells and the secretion of AFP of HepG2 cells were reduced significantly by TEA 5～160 mg· L-1. The γ-GT activity of HepG2 cells was inhibited and its ALB content was increased by TEA 20, 40 and 80 mg · L-1. The γ-GT activity of Bel-7404 cell was inhibited and its ALB content was increased byTEA 40 and 80 mg · L-1 Conclusion These results suggest that TEA has inhibitory effects on the growth, AFP secretion and γ-GT activity of human hepatoma cells.