ABSTRACT
Objective? To?observe?the?effects?of?enteral?nutrition?(EN)?emulsion?with?different?components?on?nutritional?index,?blood?glucose,?inflammatory?reaction,?gastrointestinal?tolerance?and?prognosis?in?patients?with?severe?traumatic?brain?injury?(sTBI).? Methods? Patients?with?sTBI?[Glasgow?coma?scale?(GCS)?<?8]?admitted?to?intensive?care?unit?(ICU)?of?Affiliated?Hospital?of?Guizhou?Medical?University?from?January?2016?to?May?2018?were?retrospectively?analyzed.?These?patients?had?received?EN,?30?cases?of?them?were?fed?with?Baipuli,?35?cases?were?fed?with?Ruineng,?and?30?cases?were?fed?with?Ruisu.?The?EN?support?began?within?48?hours?after?the?patients?were?transferred?to?ICU,?and?the?energy?intake?of?83.68-104.60?kJ·kg-1·d-1?was?the?nutritional?support?target.?The?hemoglobin?(Hb),?lymphocyte?count?(LYM),?serum?albumin?(Alb),?pre-albumin?(PA),?procalcitonin?(PCT),?average?blood?glucose?(GLUave)?and?blood?glucose?variation?coefficient?(GLUcv)?were?observed?7?days?after?EN?support,?EN?compliance?time,?gastrointestinal?tolerance?situations?were?recorded,?and?the?duration?of?mechanical?ventilation?(MV),?the?length?of?ICU?stay?and?incidence?of?nosocomial?infection?were?compared?among?the?three?groups.? Results? There?were?no?obvious?changes?in?Hb,?LYM,?Alb,?PCT?among?three?groups?after?treatment?on?the?7th?day,?and?there?was?no?significant?difference?among?the?three?groups.?The?PA?of?Baipuli?group?was?significantly?higher?than?that?in?Ruineng?and?Ruisu?groups?at?7?days?after?treatment?(mg/L:?275.55±46.18?vs.?238.25±49.59,?240.70±55.59,?both?P?<?0.05),?but?the?GLUave?and?GLUcv?were?much?higher?than?those?in?Ruineng?and?Ruisu?groups?[GLUave?(mmol/L):?10.02±2.39?vs.?8.53±1.53,?8.98±1.75;?GLUcv:? (23.59±3.93)%?vs.?(14.79±3.65)%,?(17.88±2.90)%,?all?P?<?0.05].?The?indexes?of?gastrointestinal?tolerance?in?Baipuli?group?were?better?than?those?of?Ruineng?and?Ruisu?groups:?the?target?feeding?time?was?reached?ahead?(days:?4.40±1.20?vs.?5.50±1.07,?5.45±1.02,?both?P?<?0.05),?the?incidence?of?gastric?retention?and?digestive?tract?hemorrhage?were?both?decreased?[6.67%?(2/30)?vs.?31.43%?(11/35),?33.33%?(10/30);?3.33%?(1/30)?vs.?14.29%?(5/35),?16.67%?(5/30),?all?P?<?0.05].?The?duration?of?MV?and?the?length?of?ICU?stay?in?Ruisu?and?Baipuli?groups?were?longer?than?those?of?Ruineng??group?[duration?of?MV?(days):?6.94±1.64,?6.79±1.14?vs.?5.93±1.12;?the?length?of?ICU?stay?(days):?9.40±2.18,?10.40±2.35?vs.?7.45±1.83,?all?P?<?0.05].?There?was?no?significant?difference?in?the?incidences?of?nosocomial?infection?among?Ruineng,?Ruisu,?and??Baipuli?groups?[the?incidence?of?ventilator-associated?pneumonia?(VAP):?11.43%,?10.00%,?10.00%;?catheter?related?bloodstream?infection:?0,?0,?3.33%;?urinary?tract?infection:?5.71%,?6.67%,?6.67%;?intracranial?infection:?2.86%,?6.67%,?3.33%,?all?P?>?0.05].? Conclusion? For?patients?with?sTBI,?short?peptide?EN?preparations?(Baipuli)?is?much?better?in?gastrointestinal?tolerance?but?it?can?cause?fluctuations?of?blood?glucose;?the?whole?protein?EN?containing?ω-3?fatty?acid?(Ruineng,?Ruisu)?has?relatively?less?fluctuation?on?blood?glucose,?the?duration?of?MV?and?the?length?of?ICU?stay?can?be?shortened?by?Ruineng,?therefore?it?is?more?suitable?to?be?applied?for?such?patients.
ABSTRACT
Objective To construct a highly efficient expression plasmid of eukaryotic nuclear membrane protein Omega 3 fatty acid desaturase gene Fat‐1 in E .coli .Methods Using molecular cloning technology to construct the recombinant prokaryotic expression plasmid pET32a Fat‐1 and pET32a‐Mistic‐Fat‐1 fused with Membrane proteins expression chaperon mistic ;the two re‐combinant plasmids were transformed into E .coli strain BL21 (DE3) ,the expression of Fat‐1 protein and M110 Fat‐1 protein in‐duced by IPTG were identified by SDS‐PAGE and gray degree analysed the amount of expression ,further identified by Western blot .Results The results of enzyme digestion and sequencing demonstrated that we successfully constructed the prokaryotic ex‐pression vectors pET32a Fat‐1 and pET32a‐Mistic‐Fat‐1;SDS‐PAGE and Western blot showed that Fat‐1 fatty acid desaturase wasn′t significantly induced ,but the overexpression of M110 Fat‐1 fusion protein was obtained in E .coli ,accounting for 15% of the total amount of whole cell proteins .Conclusion The fusion with Mistic proteins to express the Fat‐1 gene has realized the overex‐pression of eukaryotic nuclear membrane integrated protein Omega 3 fatty acid desaturase in prokaryotic host .
ABSTRACT
Objective: To investigate whether ω-3 fatty acid supplementation affects immune function, infection and surgery ratio in severe acute pancreatitis patients. Methods: Fifty-six severe acute pancreatitis patients were prospectively enrolled and were randomly assigned to receive total parenteral nutrition (TPN) for 5 days in a double-blinded manner with either soybean oil (SO) or fish oil (FO) supplementation. The level of IL-10 and HLA-DR expression were measured before TPN and on the sixth day after starting TPN. The clinical outcomes such as infection and surgery ratio were recorded. Results % Patients treated with ω-3 FA had significantly higher IL-10 concentration and better HLA-DR expression during the observation period. Although there was no significant difference between two groups, the ratio of infection and surgery ratio had a trend to decrease in the study group. Conclusion: TPN supplemented with ω-3 FA improves anti-inflammatory cytokine and immune function in SAP patients. It also leads to the attenuated risk of infection and surgery ratio.
ABSTRACT
In order to investigate the effects of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on the proliferation, apoptosis of pancreatic cancer cell line SW1990 cells and the ex-pression of cyclin E mRNA, the SW1990 cells were treated with different concentrations of EPA or DHA (20, 40, 60 μg/mL) for 0, 12, 24, 36 and 48 h respectively. By using MTT method, the inhibi-tory effects of EPA or DHA on the cell growth were assayed. Real time PCR was used to detect the expression changes of cyclin E mRNA after the SW1990 cells were treated with 40 μg/mL EPA or DHA for different time. Flow eytometry was used to test the changes of apoptostic rate in the SW1990 cells treated with different concentrations of EPA or DHA for 24 h. The results showed that EPA and DHA could inhibit the growth of SW1990 cells in a time- and concentration-dependent manner (P<0.01). EPA or DHA could also significantly inhibit the expression of cyclin E mRNA in a time-dependent manner (P<0.05). EPA or DHA could induce the apoptosis of SW1990 cells in a concentration-dependent manner (P<0.01). It was concluded that ω-3 fatty acid could inhibit the pro- liferation of pancreatic cancer cell line SW1990 cells and promote their apoptosis. The down-regulation of the cyclin E expression by ω-3 fatty acid might be one of the mechanisms for its anti-tumor effect on pancreatic cancer.