ABSTRACT
10-Deacetylbaccatin III (10-DAB) C10 acetylation is an indispensable procedure for Taxol semi-synthesis, which often requires harsh conditions. 10-Deacetylbaccatin III-10-
ABSTRACT
Objective: Two screening methods for drug active constituents were established based on the integrity of traditional Chinese medicine to screen the anti-breast cancer active components, which were hollow fiber cell fishing-high performance liquid chromatography (HFCF-HPLC) and cell fishing-dispersive liquid-liquid microextraction-high performance liquid chromatography (CF-DLLME-HPLC). Methods: According to HFCF-HPLC, MCF-7 cells were planted into the cavity of polypropylene hollow fiber, and then the hollow fiber was put into the extract of Taxus chinensis to screen and capture the bioactive component groups combined with cells in a simulated human body environment. According to CF-DLLME-HPLC, on the basis of normal cell metabolism, MCF-7 cells were co-incubated with T. chinensis extract, supernatant of different incubation time was taken for liquid phase microextraction, and then chromatographic analysis was performed to find potential active component groups according to chromatographic peak changes. Results: Anti-breast cancer active components of T. chinensis were obtained by these two methods. 10-Deacetylbaccatin III, baccatin III, taxinine M, 10-deacetyltaxol, cephalomannine, taxol, taxuyunnanine C were obtained by HFCF-HPLC, and 10-deacetyltaxol, baccatin III, taxinine M, 10-deacetyltaxol, sciadopitysin were obtained by CF-DLLME-HPLC. Conclusion: The two methods can screen the effective antitumor active ingredients, and provide a convenient, universal and efficient new method for the screening of active ingredients and the comprehensive evaluation of the quality of traditional Chinese medicine.
ABSTRACT
Objective: To increase the contents of Taxol and 10-deacetylbaccatin III in the callus of Taxus cuspidata through the application and combination of various plant growth regulators. Methods: Young stems of T. cuspidata were taken as expiants and then their effects of NAA, 2,4-D, and 6-BA on the accumulation of Taxol and 10-deacetylbaccatin III were examined with the method of orthogonal design. Results: NAA exhibited the largest effect on the yields of both total Taxol and 10-deacetylbaccatin III, and followed by 6-BA, 2,4-D. Conclusion: The optimal medium for production of total Taxol and total 10-deacetylbaccatin III is: B5+NAA 2.0 mg/L+2,4-D 0.5 mg/L+6-BA 0.2 mg/L.