ABSTRACT
Objective: To study the chemical constituents of Zhuang medicine Sauropus rostratu. Methods: The compounds were isolated and purified by silica gel, polyamide, MCI, and Sephadex LH-20 chromatography. The structures of the compounds were identified on the basis of chemical and spectral methods. Results: Nineteen compounds were isolated from 75% ethanol extrct of S. rostratu and identified as n-triacontanol (1), 2,4-di-tert-butylphenol (2), β-sitosterol (3), daucosterol (4), 3,6-anhydro-2-deoxy- D-arabino-hexono-1,4-lactone (5), niacinamide (6), scopoletin (7), 3β-hydroxy-26α,27β-arbor-13(18)-ene-24-carboxylic acid (8), 2R*,3R*,5S*-trihydroxy-6R*-nonadecyltetrahydropyran-4-one (9), emodin (10), protocatechuic acid (11), 2,3-dideoxy-D-erythro- hex-2-enono-1,4-lactone (12), caffeic acid (13), 3-acetrlc-affeic acid (14), quercetin (15), D-galactose (16), mannitol (17), aurantiamide acetate (18), and kaempferol (19). Conclusion: Compound 8 is a new compound named sauropurostratic acid, and compounds 2,5-14,and 16-18 are isolated from this plant for the first time.
ABSTRACT
Streptomyces sp. KB1(TISTR 2304), identified by 16S rDNA gene, was collected from the air sample at Ao-nang, Krabi province, Thailand and deposited in the GeneBank database (accession number KF939581.1). It could produce bioactive compounds and excrete into liquid culture medium within 4 days of incubation period at 30 °C, 200 rounds per minute in shaking incubator. Bioactive compounds in culture broth supernatant were extracted with 3-times of ethyl acetate (EA), evaporated by rotary evaporator at 45 °C under reduced pressure, purified by silica gel column chromatography and eluted by gradient solvent system of ethyl acetate: hexane at ratio of 1:9, 3:7, 4:6, 6:4 and 10:0, v/v. Eight pooled fractions were obtained, name PF1 - PF8, from basis of their TLC profile. Only PF4 displayed as purified active compound which showed anti-methicillin resistant Staphylococcus aureus (MRSA) activity. The PF4 was predicted as 2, 4-Di-tert-butylphenol (C14H22O) with molecular weight 206.2.