ABSTRACT
Objective: To study the chemical constituents from the roots and rhizomes of Glycyrrhiza uralensis. Methods: The roots and rhizome of G. uralensis were extracted with 95% EtOH. Then the extract was partitioned with petroleum ether, ethyl acetate, and n-butanol successively. The n-butanol fraction was isolated by silica gel and ODS column chromatography and preparative HPLC. The structures of compounds were identified by spectroscopic methods (UV, MS, 1D-NMR, and 2D-NMR). Results: Fourteen compounds were obtained from the n-butanol fraction. They were macedonoside E (1), 22β-acetyl-uralsaponin C (2), glycyrrhizic acid (3), uralsaponin F (4), licorice-saponin G2 (5), 22β-acetoxyl-glycyrrhaldehyde (6), 3β-O-[β-D-(6-methyl)-glucuronopyranosyl-(1→2)-β-D-glucuronopyranosyl]-glycyrrhizic acid (7), liquiritigenin (8), naringenin (9), isoliquiritigenin (10), ononin (11), liquiritin (12), isoviolanthin (13), and liquiritin apioside (14). Conclusion: Macedonoside E (1) and 22β-acetyl-uralsaponin C (2) are new compounds.