ABSTRACT
Objective: To study the chemical constituents of aerial part of Gendarussa vulgaris. Methods: The compounds were separated and purified by silica gel column chromatography, ODS and Sephadex LH-20 chromatography and semi-preparative HPLC. Base on HR-ESI-MS, NMR, and other spectral data, their structures were identified. Results: A total of 17 compounds were isolated from the EtOAc fraction of 95% ethanol extract and identified as 24-norchol-5-en-3β-ol (1), dihydrobetulic acid (2), betulinic acid (3), 3-hydroxy-30-nor-20-oxo-28-lupanoic acid (4), 6-hydroxy-7,8-dimethoxycoumarin (5), 6,7-dimethoxycoumarin (6), 5,6,7- trimethoxycoumarin (7), 6,7,8-trimethoxycoumarin (8), syringaresinol-4-O-β-D-glucopyranoside (9), 4-O-caffeoylquinic acid methyl ester (10), N-trans-feruloyl tyramine (11), N-(2-hydroxy-3-phenylpropyl) acetamide (12), 3-O-caffeoylquinic acid methyl ester (13), 3,5-O-dicaffeoylquinic acid methyl ester (14), p-E-coumarin quinic acid methyl ester (15), 3,4,5-O-tricaffeoyl quinic acid methyl ester (16) and 1'S*,4'R*-8-(4'-hydroxy-2',6',6'-trimethylcyclohex-2-enyl)-6-methyloct-3E,5E,7E-trien-2-one (17). Conclusion: Compounds 1 and 2 are new natural products. All Compounds are isolated from this plant for the first time except compound 6. Besides, all compounds are screened for anti-inflammatory activity and compounds 2, 3, 11, 13, and 17 have NO release inhibiting activities on LPS-induced RAW 264.7 macrophage cells with IC50 values of (30.91 ± 0.50), (44.66 ± 0.56), (17.67 ± 0.57), (28.45 ± 0.67) and (20.79 ± 0.24) μmol/L, respectively.
ABSTRACT
Objective: To study the chemical constituents of Periploca Radix. Methods: The chemical constituents of Periploca forrestii were separated using various chromatographic techniques. Their structures were elucidated by spectral analysis. Results: Nineteen compounds were isolated from the 70% ethanol extract of P. forrestii, and identified as 3-O-caffeoylquinic acid methyl ester (1), 4-O-caffeoylquinic acid methyl ester (2), 5-O-caffeoylquinic acid methyl ester (3), 3-O-caffeoylquinic acid (4), 4-O-caffeoylquinic acid (5), 5-O-caffeoylquinic acid (6), 1, 3-di-O-caffeoylquinic acid (7), 3, 4-di-O-caffeoylquinic acid (8), 3, 5-di-O- caffeoylquinic acid (9), 4, 5-di-O-caffeoylquinic acid (10), protocatechuic aldehyde (11), p-hydroxybenzoic acid (12), o-hydroxybenzoic acid (13), syringic acid (14), vanillic acid (15), periforgenin A (16), Δ5-pregnene-3β, 17α, 20α-triol (17), periforgenin C (18), and periplogenin (19). Conclusion: Compounds 1-12 are isolated from the plants of genus Periploca Linn. for the first time, and compound 13 is isolated from P. forrestii for the first time.
ABSTRACT
Objective: To study the chemical constituents from the 75% ethanol extract of Euodia rutaecarpa. Methods: Chromatographic methods, such as silica gel, ODS, and Sephadex LH-20 column chromatography, were used for the isolation and purification. The structures were identified on the basis of spectroscopic analysis. Results: Eight compounds were isolated from the 75% ethanol extract of E. rutaecarpa, and were identified as sinapyl alcohol 9-O-feruloyl-4-O-β-D-glucopyanoside (1), 3-O- caffeoylquinic acid methyl ester (2), caffeic acid (3), ferulic acid (4), p-hydroxycinnamic acid (5), 4-methoxybenzylalcohol (6), 3, 4-dihydroxy-benzoic acid (7), and 7-hydroxy coumarin (8). Conclusion: Compound 1 is a new phenylpropanoid glycoside named neoeuodiside, and compounds 2, 3, 6, and 7 are isolated from the plants of Euodia J. R. et G. Forst. for the first time.