ABSTRACT
Objective: To research the chemical composition from the roots of Lonicera japonica and their anti-inflammatory activities. Methods: The compounds were isolated and purified by chromatography on silica gel column, Sephadex LH-20 column, preparative thin-layer, and semi-preparative HPLC, etc. Their structures were identified on the basis of spectroscopic data, and parts of the isolated compounds were tested for their anti-inflammatory activities against zebrafish. Results: Fourteen compounds were isolated and elucidated as 3,13-dihydroxystemodan-2-one (1), chrysophanol (2), palmarumycin CP2 (3), β-sitosterol (4), stigmasterol (5), stigmast-4,6,8(14), 22-tetraen-3-one (6), erythrinassinate D (7), lanosterin (8), 3-O-acetyloleanolic acid (9), protocatechuin aldehyde (10), daucosterol (11), (E)-3-(3,4-dihydroxybenzylidene)-5-(3,4-dihydroxyphenyl)-2(3H)-furanone (12), lomacarinoside B (13), and (2E,6S)-8-(α-L-arabinopyranosyl-(1″→6')-β-D-glucopyranosyloxy)-2,6-dimethyloct-2-eno-1,2″-lactone (14). Conclusion: Compound 1 is a new compound, and compounds 2, 3, 6, 7-9, and 13 are isolated from L. japonica for the first time. Compounds 9 and 10 show the significant anti-inflammatory activities at 100 μg/mL.
ABSTRACT
Objective: To study the low-polarity chemical constituents from the whole plants of Scrophularia dentata grown in Tibet. Methods: The compounds were isolated and purified by various column chromatography. Their structures were elucidated by means of spectral analyses (MS, 1H-NMR, and 13C-NMR). Results: Fifteen compounds were isolated and identified as oleanolic acid (1), 3-O-acetyloleanolic acid (2), stigmast-4-en-3-one (3), stigmast-4, 22-dien-3-one (4), 2, 6-dimethoxybenzoquinone (5), cerevisterol (6), cinnamic acid (7), spinasterol (8), β-sitosterol (9), 2-tritriacontanone (10), 2-(4-hydroxyphen-yl)-ethyl 1-dodecyloctadecanoate (11), apigenin (12), chrysoeriol (13), medioresinol (14), and syringaresinol (15). Conclusion: All the compounds are firstly isolated from this plant and compounds 2-6 and 10-15 are isolated from the plants of this genus for the first time.