ABSTRACT
Objective: To investigate the phenanthrenes from chloroform fraction of Dendrobium chrysanthum. Methods: Several chromatographic methods such as Sephadex LH-20 and semi-preparative HPLC were applied to isolating and purifying compounds from the antihyperglycemic chloroform fraction of D. chrysanthum. Results: The structures were identified on the basis of physicochemical properties and spectroscopic data. Four compounds were isolated and identified as 2, 5-dihydroxy-4-methoxy- 9, 10-dihydrophenanthrene (1), 2, 4-dihydroxy-5-methoxy-9, 10-dihydrophenanthrene (2), 2, 4, 5-trihydroxy-9, 10-dihydrophenanthrene (3), and loddigesiinol A (4). Conclusion: Compound 2 is a new phenanthrene, named as chrysanthin A. Compounds 1, 3, and 4 are isolated from D. chrysanthum for the first time.
ABSTRACT
The present study was designed to identify bioactive compounds similar to those isolated from Dendrobium genus from its relative specie Eria bambusifolia. Compounds 1-10 were isolated and purified using silica gel, MCI CHP-20 gel, Sephadex LH-20, and Lichroprep RP-18 chromatography methods. Their structures were elucidated by means of extensive spectroscopic analyses. The cytotoxicity of these compounds against five human cancer cell lines was tested. Erathrins A and B (1 and 2) were new compounds, and compound 1 represented a novel carbon framework having a phenanthrene-phenylpropane unit with a dioxane moiety. Moreover, compound 1 showed selective cytotoxic activity against HL-60 cells (IC50 = 14.50 μmol·L(-1)). These results provided a basis for future development of these agents as anticancer lead compounds.
Subject(s)
Humans , Antineoplastic Agents, Phytogenic , Chemistry , Pharmacology , Cell Survival , Dendrobium , Chemistry , HL-60 Cells , Molecular Structure , Phenanthrenes , Chemistry , Pharmacology , Plant Extracts , Chemistry , PharmacologyABSTRACT
A detailed chemical analysis of the non-volatile secondary metabolites of Encyclia longifolia Schltr. syn. Epidendrum longifolium Barb. Rodr. was carried out in an attempt to lend a chemotaxonomic support for the recent separation of the genus Encyclia into Encyclia Hook and Prosthechea Knowles & Westc.
Uma análise química detalhada dos metabólitos secundários não voláteis de Encyclia longifolia Schltr. Sin. Epidendrum longifolium Barb. Rodr. foi realizada na tentativa de prestar suporte quimiotaxonômico para a recente separação do gênero Encyclia em Encyclia Hook e Prosthechea Knowles & Westc.