A comparative study of radionuclide imaging technique diagnosis and surgical results for Meckel's diverticulum in children / 临床儿科杂志

Objective To evaluate the accuracy of 99mTcO4- gastrointestinal imaging in the diagnosis of Meckel’s diverticulum in children. Methods The clinical data of 99mTcO4-imaging, surgery and pathological results of 95 children with gastrointestinal bleeding were retrospectively analyzed. Results Forty-four cases of 95 patients had positive ifnding of 99mTcO4-ectopic gastric mucous membrane imaging, and positive rate was 46.3%. In the positve cases 52.3%cases (23/44) were diagnosed of intestinal heterotopic gastric mucosa, 47.7%cases (21/44) were suspected of intestinal heterotopic gastric mucosa. The sex ratio (males to females) was 7.8:1. Thirty-seven positive cases underwent operation;among them, 35 cases were found to have Meckel’s diverticulum and 1 case had duplication of the digestive tract;Diverticulum lesion was not found in 1 case. The samples of 35 cases diagnosed surgically of Meckel’s diverticulum were examined by pathologic histology. All 35 cases were conifrmed as Meckel’s diverticulum and ectopic gastric mucosa epithelium, including 2 cases with ulcer formation, 1 case with small patches of pancreatic tissue, 1 case with ileocecal junction of suppurative enteritis and 1 case with chronic appendicitis. Conclusions 99mTcO4-radionuclide imaging technique is of high value for the non-invasive diagnosis of children’s Meckel’s diverticulum.

Study on hNIS mediated transferring gene99mTcO-4 imaging and mI treatment in xenografted ovariancancer / 中华内分泌代谢杂志

Objective To establish radioiodine therapy in nonthyroid tumor and to investigate 131Ⅰ treatment effect on xenografted ovarian cancer. Methods Based on previous test, xenografted ovarian cancer nude model were established in nude mice. The effects of radioactive isotope 99m TcO-4 imaging and radioiodine 131Ⅰ treatment on xenografted ovarian cancer in vivo were investigated. Results After transferring human sodium/iodide symporter (hNIS) gene, the xenografted ovarian cancer in nude mice was imaged by isotope 99m TcO-4 Moreover,131Ⅰ exerted inhibitory effect on the proliferative activity. Conclusion After the transfection of hNIS gene, 131Ⅰ has inhibitory effect on proliferative activity of xenografted ovarian cancer.

Effect of paclitaxel (Taxol®) on the biodistribution of sodium pertechnetate (Na99mTcO4) in female Wistar rats

The evidence that natural or synthetic drugs can affect the biodistribution of radiopharmaceuticals (radiobiocomplexes) in setting of nuclear medicine clinic is already known. We studied the effect of Paclitaxel, an anti-neoplastic agent for the treatment of solid tumors, on the biodistribution of Na99mTcO4 in female rats. Paclitaxel (1mg/mL/week) was administered into animals in single dose during 3 weeks, with interval of 1 weekamong them. The control group received NaCl 0.9% solutions by the same via. One hour after the last dose, it wasinjected Na99mTcO4 in the animals. The percentage of activity per gram (% ATI/g) and biochemical andhematological determinations were performed. A significant increase were found in alanine aminotransferase,aspartate aminotransferase, glucose and in the %ATI/g of some organs (ovaries, uterus, vagina, breasts, large intestine and liver).These results can be associated, probably, to the capacity of paclitaxel to alter the biodistribution of Na99mTcO4 and the metabolism of glucose and hepatic enzymes.

Já está bem estabelecido na literatura científica que produtos naturais ou sintéticos podem alterar a biodistribuição de radiofármacos. O objetivo desse estudo foi avaliar a influência do paclitaxel, um agente antineoplásico para tratamento de tumores sólidos na biodistribuição do pertecnetato de sódio em ratas Wistar e na determinação de componentes bioquímicos e hematológicos. Paclitaxel, comercialmente conhecido por Taxol®(1mg/mL/semana), foi administrado,intraperitoneamente, nos animais do grupo tratado, em dose única, por 3 semanas, mas com intervalo de uma semana entre elas. O grupo controlerecebeu solução de NaCl 0,9%. Uma hora após a última dose de paclitaxel, os animais receberam 0,1 mL de Na99mTcO4 (3,7MBq) via plexo orbital.O percentual de radioatividade por grama (%ATI/g) e parâmetros laboratoriais foram determinados. Ocorreu um aumento significativo (p<0,05) do %ATI/g nos ovários, útero, vagina, mamas, intestino grosso e fígado. Os níveis de glicose sangüínea e das enzimas hepáticas (ALT e AST) também aumentaram significantemente(p<0,01). Esses resultados podem estar associados, provavelmente, à capacidade do paclitaxel em alterar a biodistribuição do Na99mTcO4 e ometabolismo da glicose e de enzimas hepáticas.