IMMUNOHISTOCHEMICAL LOCALIZATION OF ENDOTHELIN-LIKE IMMUNOREACTIVE SUBSTANCE IN THE ENDOTHELIAL CELLS OF HUMAN UMBILICAL VEINS AND PORCINE AORTA / 解剖学报

Endothelin (ET), recently isolated from culture supernatant of porcine aortic endothelial cells, is a novel potent vasoconstrictive substance. In this paper a highly specific rabbit antisera against endothelin was used to study the localization of ET in the isolated endothelial cells, from human umbilical veins and porcine arota, and that of ET in the cryostat cross sections of porcine aorta by using ABC immunohistochemical technique. The results demonstrate that a varied amounts of specific ET-like immunoreactive substance exist in the endothelial cytoplasm of human umbilical veins and porcine aorta which provides the mor- phological evidence for elucidating the endocrine function of vascular endothelial cells.