ABSTRACT
Objective To compare the flow cytometry versus test tube method in detecting antibody titer in ABO blood type,and try to establish a standard method using flow cytometry to provide insight in ABO incompatible kidney transplantation and therapeutics.Methods The ABO blood type titers of anti-IgM-A/B and anti-IgG-A/B in 30 serum samples from renal allograft recipients were measured by flow cytometry.The results were compared with those determined by test tube method.Results The titers by cytometry significantly higher than those by test tube method (P＜0.05).Conclusion The sensitivity of flow cytometry is significantly higher than test tube method,and flow cytometry can precisely monitor the ABO blood titers in renal allograft recipients,which can provide better medical advice in clinical treatment and therapeutics.