Evaluation of CELL-DYN Sapphire Hematology Analyzer / 임상검사와정도관리

BACKGROUND: CELL-DYN Sapphire (Abbott Diagnostics, Santa Clara, CA, USA) hematology analyzer is based on laser multi angle polarized scatter separation, focused flow impedance and fluorescent flow cytometry. In order to assess the usefulness of this newly introduced analyzer, we evaluated the precision and correlation of complete blood count including white blood cell differential count and reticulocyte count of CELL-DYN Sapphire instrument. METHODS: Patient samples ordered for complete blood count and white blood cell differential count and CELL-DYN 29 Plus control samples were used. We evaluated the precision of complete blood count and WBC differential count and analyzed correlation of these parameters for comparison with ADVIA 120 (Bayer corporation, Tarrytown, NY, USA) and manual count. We additionally evaluated reticulocyte parameters for precision and comparison with ADVIA 120. RESULTS: Short term precisions showed low coefficient of variation (CV) values for all CBC parameters including lower than 3% CV values for neutrophil and lymphocyte count. Long term precision showed lower than 4% CV values for all CBC and WBC differential count except for basophils. Comparison with ADVIA 120 showed good correlation for CBC except for MCHC. Good correlation was confirmed in all differential counts except for basophils. Comparison with manual WBC differential count showed good correlation except for monocyte and eosinophil. CONCLUSIONS: CELL-DYN Sapphire has high precision and good correlation with ADVIA 120 and manual count. CELL-DYN Sapphire is an exceptional instrument for CBC with WBC differentials and reticulocyte counting.

Evaluation of CELL-DYN Sapphire Hematology Analyzer / 임상검사와정도관리

BACKGROUND: CELL-DYN Sapphire (Abbott Diagnostics, Santa Clara, CA, USA) hematology analyzer is based on laser multi angle polarized scatter separation, focused flow impedance and fluorescent flow cytometry. In order to assess the usefulness of this newly introduced analyzer, we evaluated the precision and correlation of complete blood count including white blood cell differential count and reticulocyte count of CELL-DYN Sapphire instrument. METHODS: Patient samples ordered for complete blood count and white blood cell differential count and CELL-DYN 29 Plus control samples were used. We evaluated the precision of complete blood count and WBC differential count and analyzed correlation of these parameters for comparison with ADVIA 120 (Bayer corporation, Tarrytown, NY, USA) and manual count. We additionally evaluated reticulocyte parameters for precision and comparison with ADVIA 120. RESULTS: Short term precisions showed low coefficient of variation (CV) values for all CBC parameters including lower than 3% CV values for neutrophil and lymphocyte count. Long term precision showed lower than 4% CV values for all CBC and WBC differential count except for basophils. Comparison with ADVIA 120 showed good correlation for CBC except for MCHC. Good correlation was confirmed in all differential counts except for basophils. Comparison with manual WBC differential count showed good correlation except for monocyte and eosinophil. CONCLUSIONS: CELL-DYN Sapphire has high precision and good correlation with ADVIA 120 and manual count. CELL-DYN Sapphire is an exceptional instrument for CBC with WBC differentials and reticulocyte counting.

Comparison of conventional manual methods with the ADVIA 120 automated method for counting of red and white blood cells in cerebrospinal fluid.

Objective: To evaluate the correlation and agreement of erythrocyte and leukocytes count in cerebrospinal fluid (CSF) among two different manual methods and automated method. Methods: We evaluated the correlations and agreements of the CSF RBC counts, WBC counts and WBC differential counts between two manual methods and automated method by using the ADVIA 120 CSF assay. Results: We studied 83 CSF specimens in all methods. Absolute cell counts showed a high correlation and agreement between methods, with correlation coefficient (rs) for all absolute counts of more than 0.89 and intraclass correlation (ICC) more than 0.9. The correlation and agreement of WBC differential counts from CSF specimens which had more than 20 WBCs/µL were also evaluated, which revealed good results only for polymorphonuclear cells, neutrophils and lymphocytes (rs = 0.796, 0.835 and 0.779, respectively and ICC = 0.954, 0.899 and 0.907, respectively). When WBC counts more than 5 cells/µL in automated method were used as a cut-off point, the sensitivity is 100% but specificity is very low (60.87%). The cut-off point of 5 WBCs/µL for manual method and 11 WBCs/µL for automated method gave the highest agreement (Kappa 0.874, sensitivity 91.43% and specificity 95.65%). Conclusion: The ADVIA 120 CSF assay provide a useful and efficient method for excluding the normal CSF specimens at cut-off 5 WBCs/µL.