ABSTRACT
The Atlantic and Gulf Rapid Reef Assessment (AGRRA) Program, consisting of non-fixed, transect- based visual surveys, provides "snapshot" characterizations of a number of structurally or functionally important benthic and fish indicators on tropical northwestern Atlantic (TNWA) coral reefs and Brazil. To ameliorate the confounding factors inherent in multiyear temporal variation, a region-wide collaborative program for conducting rapid reef assessments at regular (five-year) intervals in sites that are representative of each sub-region of the TNWA is proposed. Member institutions of the Association of Marine Laboratories of the Caribbean (AMLC) and individual colleagues are invited to join in developing the vision. Rev. Biol. Trop. 54 (Suppl. 3): 23-29. Epub 2007 Jan. 15.
El Programa de Evaluación Rápida del Atlántico y el Golfo (AGRRA, por sus siglas en inglés) consiste en sondeos visuales no-fijos, basados en transectos, que permiten caracterizaciones puntuales de un número de peces y organismos bénticos que sean estructural o funcionalmente importantes como indicadores en arrecifes del Atlántico tropical noroeste (ATNO) y de Brasil. Para disminuir los factores de confusión inherentes a variaciones temporales multianuales, se propone un programa de colaboración a nivel regional, que sirva para conducir evaluaciones rápidas de los arrecifes en intervalos regulares (cinco años), en los sitios que sean representativos de cada sub-región del ATNO. Se invita a las instituciones miembro de la Asociación de Laboratorios Marinos y del Caribe (ALMC), así cómo a colegas individuales para participar en el desarrollo de esta visión.
Subject(s)
Audiovisual Aids , Environmental Monitoring , Evaluation Studies as Topic , Coral ReefsABSTRACT
Objective:To isolate and establish long term T cell clones derived from bone marrow(BM) of a patient with severe aplastic anemia(sAA).Methods:CD34 + cells and CD3 +T cells were enriched from BM aspirates,respectively by positive and negative immunomagnetic sorting protocol.BM of exicised rib of a non hematological patient was used as control. Irradiated CD34 + cells together with T cells were subject to one way autologous mixed lymphocyte culture(AMLC).Viable T cells surviving from AMLC were limit diluted and single cell was plated into microtitier wells along with feeder cells.Then all wells were screened for the growing cell wells and they were transferred into new wells to be expanded and maintained.Each clones obtained were primarily assessed CD4/CD8 phenotype and their homogeneity by immunocytochemical analysis.Results:The purity of sorted CD34 + and T cells was more than 90%. On AMLC,a total of 4?10 3 cells were viable with sAA,but 65 cells viable with control.Upon single cell seeding it 11 growing cell wells were found for sAA,and no one growing cells for control.The actual plating rate(2.1%) was within Poisson theoretic value range (