Research progress in hematopoietic stem cell transplantation for bone marrow acute radiation syndrome / 中国辐射卫生

As is known, the nuclear accident resulting from the explosion of a nuclear weapon or the release of nuclear material could cause acute radiation syndrome within a short time. The study had found that the dose of > 0.7 Gy radiation on human body can result in persistent myelosuppression, a kind of acute radiation syndrome, leading to pancytopenia, bleeding, infection and other injuries. Several evidences also have shown that hematopoietic stem cell is conducive to repair hematopoietic injury in bone marrow, improve hematopoietic microenvironment and promote hematopoietic reconstruction. Therefore, hematopoietic stem cell transplantation is widely considered as the main treatment of the bone marrow acute radiation syndrome. However, before a surgery of hematopoietic stem cell transplantation, it still needs more research on donor selection, formulation of preconditioning and prevention of complications such as graft-versus-host disease. This paper mainly summarizes the application and research progress of hematopoietic stem cell transplantation in treating radiation injury.

Instrumentos e integração de políticas públicas: a rede do Cadastro Único / Instrumentos e integración de políticas públicas: la red del Registro Único / Instruments and public policy integration: the network of the Single Registry for Social Programs

Resumo Este artigo analisa a capacidade de integração de políticas públicas suportadas por um mesmo instrumento de implementação. Partindo da abordagem dos estudos de implementação de políticas públicas que compreendem os instrumentos como "o estado em ação", isto é, ferramentas de ação pública por meio das quais a ação governamental se materializa e é operacionalizada (Lascoumes & Le Galès, 2007; Linder & Peters, 1990), examina-se o caso da Rede do Cadastro Único para Programas Sociais, formada pelo instrumento do Cadastro Único - composto por sua base de dados, normas, processos e estrutura municipal de cadastramento e coleta de dados - e pelos 20 programas sociais que se utilizam de suas informações cadastrais sobre 27 milhões de famílias, quase 80 milhões de pessoas de baixa renda, para seleção de beneficiários e gerenciamento de políticas voltadas ao combate das diferentes formas de vulnerabilidade social. Adotou-se a metodologia da análise de redes sociais para responder a duas perguntas principais: qual é a posição ocupada pelo Cadastro Único (CadÚnico) na rede de relações formada com as políticas sociais que o utilizam e quais são as condições e o nível de integração que o CadÚnico promove entre essas políticas usuárias? As métricas e os sociogramas de dois contextos temporais distintos, 2016 e 2018, demonstraram que, embora haja potencial para que promova maior integração entre as políticas, o CadÚnico ainda não se apresenta como instrumento potente de integração de políticas públicas.

Resumen Este artículo analiza la capacidad de integración de políticas públicas sustentadas por un mismo instrumento de implementación. Partiendo del enfoque de los estudios de implementación de políticas públicas que comprenden instrumentos como "el estado en acción", es decir, herramientas de acción pública por medio de las cuales la acción gubernamental se materializa y se pone en marcha (Lascoumes & Le Galès, 2007; Linder & Peters, 1990), se examina el caso de la Red del Registro Único para Programas Sociales, formada por su base de datos, normas, procesos y estructura municipal de registro y recolección de datos y también por los 20 programas sociales que utilizan la información de registro de 27 millones de familias, casi 80 millones de personas de bajos ingresos, para selección de beneficiarios y gestión de políticas dirigidas al combate de las diferentes formas de vulnerabilidad social. Se adoptó la metodología de Análisis de Redes Sociales para responder a dos preguntas principales: 1) ¿Cuál es la posición que ocupa el Registro Único en la red de relaciones formada con las políticas sociales que lo utilizan? 2) ¿Cuáles son las posibilidades y el nivel de integración que el Registro Único promueve entre esas políticas? Las métricas y sociogramas de dos contextos temporales distintos ‒2016 y 2018‒ demostraron que, aunque haya potencial para que promueva una mayor integración entre las políticas, el Registro Único todavía no se presenta como instrumento potente de integración de políticas públicas.

Abstract This article analyzes the capacity of policy integration through implementation tools. The research builds on studies on policy implementation, which understand such tools as "the state in action," i.e., instruments through which governments operate and deliver public services (Lascoumes & Le Galès, 2007; Linder & Peters, 1990). The research examines the case of the Brazilian Single Registry for Social Programs (Cadastro Único or CadUnico). The Single Registry is understood as part of a network providing social protection to the Brazilian population in social vulnerability. This work examines CadUnico as a complex system formed of a database and a set of norms and processes that work within the structure of local governments. The system gathers data used to coordinate 20 social programs, helping to select beneficiaries and manage policies that serve 27 million low-income families, reaching almost 80 million people. We adopted the social network analysis as a research method, seeking to answer two main questions: what is the position occupied by the Single Registry in the network of relationships formed by the federal social policies which use it, and what are the conditions and the level of integration which it promotes among these federal user policies? The metrics and sociograms of two distinct timeframes, 2016 and 2018, have demonstrated that, although the Single Register for Social Programs offers elements to promote some integration among public policies, there is no evidence of its effectiveness to this end.

El 31 de diciembre de 2019 las autoridades de la República Popular China comunicaron a la OMS varios casos de neumonía de etiología desconocida en Wuhan, una ciudad situada en la provincia China de Hubei. [Editorial] / On December 31, 2019, the authorities of the People's Republic of China reported to the WHO several cases of pneumonia of unknown etiology in Wuhan, a city located in the Chinese province of Hubei. [Editorial]

Resumen El 31 de diciembre de 2019 las autoridades de la República Popular China comunicaron a la OMS varios casos de neumonía de etiología desconocida en Wuhan, una ciudad situada en la provincia China de Hubei. Una semana más tarde confirmaron que se trataba de un nuevo coronavirus que ha sido denominado SARS-CoV-2 (coronavirus de tipo 2 causante del síndrome respiratorio agudo severo, del inglés, Severe acute respiratory syndrome coronavirus. Este nuevo virus causa diversos cuadros clínicos englobados bajo el término COVID-19 (enfermedad por coronavirus 2019, del inglés, coronavirus disease 2019), e incluyen desde infección asintomática, a cuadros respiratorios leves con tos, malestar general y fiebre hasta cuadros de neumonía grave con síndrome de distrés respiratorio agudo, shock séptico y fallo multi-orgánico y muerte. La mayoría de los casos de COVID-19 notificados hasta el momento cursan con cuadros leves; no obstante, tanto el pronóstico de la enfermedad como el conocimiento actual, basado en los datos más ecientes publicados y los pacientes ya tratados, indican que un porcentaje significativo de pacientes cursaran con insuficiencia respiratoria aguda, neumonía intersticial e hipoxemia grave que requieren ventilación mecánica prolongada y presentan una mortalidad elevada. La expansión del virus a todo el planeta, su capacidad de difusión y su infectividad, el elevado número de afectados en crecimiento exponencial, una mortalidad creciente con la edad y la comorbilidad de los afectados, así como su impacto sobre la vida de las personas y el sistema de asistencia sanitaria han provocado una emergencia sanitaria mundial de grandes proporciones. La Coordinación General de Docencia, conjuntamente con los diferentes servicios de esta casa de salud, participó de una manera activa y actualizada con información referente al COVID-19, destacamos los siguientes temas: Imagenología en tiempos de pandemia; embarazo y covid-19; miocardiopatía y COVID-19; planificación de una unidad de reanimación ante una pandemia y experiencia clínica; utilidad del ultrasonido en unidades críticas COVID-19; recomendaciones y experiencias para ecocardiografía y eco pulmonar en tiempos de COVID-19; cirugía plástica y quemaduras durante la pandemia; Kawasaki-like y COVID-19; VIH y COVID-19 historia natural; hemodiálisis en tiempos de COVID-19; plasma convaleciente e inmunidad en pacientes COVID-19; prevención y vacuna en COVID-19; abordaje del recién nacido con mal formación cardiaca: tratamiento sin cirugía por cateterismo; soporte nutricional en el paciente COVID-19 crítico; eventos trombo-embolicos en pacientes COVID-19, rescate vascular cerebral en tiempos COVID-19; desafíos actuales en el manejo de infarto agudo de miocardio en tiempos de pandemia; salud mental en tiempos de COVID-19; desde la fisiopatología y Medicina Basada en Evidencias (MBE) a la práctica clínica; mensajes clave para el manejo integral del paciente grave con Insuficiencia Renal Aguda (IRA) por COVID-19; experiencias y manejo del malestar emocional durante la pandemia COVID-19; Hipertensión Arterial: análisis de las últimas Felicitamos a todos los participantes y expresamos nuestro profundo reconocimiento por su vocación de servicio y entrega para enfrentar la crisis sanitaria, asumir su rol social y constituirse en sujeto social y político, que interpreta adecuadamente la realidad y plantea soluciones que contribuyen significativamente a la resolución de los problemas sociales; cada detalle cuenta para buscar una forma de contribuir como empresa cuya misión es la Salud. La Pandemia por COVID-19 ha ocasionado grandes impactos en nuestra sociedad y nos ha entristecido en lo más profundo la partida de innumerables colegas que nos dejan huella de bondad, lealtad, solidaridad y amistad, nuestro sentido pésame a sus distinguidas familias. (au)

Mizoribine treatment in an elderly diabetic patient with antisynthetase-associated interstitial lung disease / Journal of Rural Medicine

Objective: Immunosuppressive therapy for interstitial lung disease (ILD) is often necessary, but the standard regimen for antisynthetase-associated ILD has not been established.Patient: An 80-year-old man was hospitalized for severely progressive dyspnea. Bilateral interstitial shadows occurred 1 month before the event. Serological findings showed that he had antisynthetase-associated ILD, as identified by strong positivity for anti-aminoacyl-transfer RNA synthetase (ARS) antibody, despite no evidence of myositis. He was treated transiently with noninvasive positive pressure ventilation and steroid-pulse therapy followed by 60 mg/day of oral prednisolone. However, his diabetes mellitus was aggravated by corticosteroid therapy; thus, a combination of low-dose steroid and mizoribine (MZB), which has a low risk of aggravating glucose intolerance, was used.Results: The patient’s clinical symptoms and daily life activities have been well persevered as an outpatient and well maintained with 200 mg of MZB and 10 mg of prednisolone for several months without obvious clinical recurrence and without any remarkable steroid- and MZB-related side effects.Conclusion: The use of MZB appeared to suppress the pathophysiology of anti-ARS antibody-associated ILD.

Synergistic antitumor activity of artesunate and HDAC inhibitors through elevating heme synthesis synergistic upregulation of ALAS1 expression

Artemisinin and its derivatives (ARTs) were reported to display heme-dependent antitumor activity. On the other hand, histone deacetylase inhibitors (HDACi) were known to be able to promote heme synthesis in erythroid cells. Nevertheless, the effect of HDACi on heme homeostasis in non-erythrocytes remains unknown. We envisioned that the combination of HDACi and artesunate (ARS) might have synergistic antitumor activity through modulating heme synthesis. studies revealed that combination of ARS and HDACi exerted synergistic tumor inhibition by inducing cell death. Moreover, this combination exhibited more effective antitumor activity than either ARS or HDACi monotherapy in xenograft models without apparent toxicity. Importantly, mechanistic studies revealed that HDACi coordinated with ARS to increase 5-aminolevulinate synthase (ALAS1) expression, and subsequent heme production, leading to enhanced cytotoxicity of ARS. Notably, knocking down significantly blunted the synergistic effect of ARS and HDACi on tumor inhibition, indicating a critical role of ALAS1 upregulation in mediating ARS cytotoxicity. Collectively, our study revealed the mechanism of synergistic antitumor action of ARS and HDACi. This finding indicates that modulation of heme synthesis pathway by the combination based on ARTs and other heme synthesis modulators represents a promising therapeutic approach to solid tumors.

Association between miR146a(rs2910164)G>C polymorphism and susceptibility to acute lymphoblastic leuke-mia in children / 中华实用儿科临床杂志

Objective To investigate the association between miR146a(rs2910164)G>C polymorphism and susceptibility to acute lymphoblastic leukemia(ALL)in children.Methods Two hundred blood specimens were ob-tained from children with ALL as patient group and 100 blood specimens were obtained from healthy children as healthy control group,who were all from Baoding First Central Hospital between March 2010 and October 2016.There were no significant differences in sex and age between patient group and healthy control group(χ2=0.430,P=0.512;χ2=2.839,P=0.092).The distribution of gene frequency of patient group and healthy control group conformed to Hardy-Weinberg equilibrium.miR146a(rs2910164)G>C polymorphism was identified by adopting restriction fragment length polymorphism(RFLP).The relation of genotype and ALL was demonstrated by odds ratio(OR)and 95% credibility interval(CI). Results Gene frequency of miR146a(rs2910164)GG,GC and CC genotypes in patient group and healthy control group was 16.0%,44.5%,39.5% and 29.0%,41.0%,30.0%,respectively.The GC/CC genotypes were significantly higher in patient group than those in healthy control group(GG genotype as reference,GC genotype:OR=1.967,95%CI:1.054-3.672,P=0.037;CC genotype:OR=2.386,95%CI:1.239-4.595,P =0.012). Conclusion miR146a(rs2910164)G>C polymorphism is significantly associated with susceptibility to ALL in chil-dren.

Effects of 5-HT1A receptor in hippocampal dentate gyrus on anxiety-related behavior of adult rats with Parkinson's disease / 西安交通大学学报(医学版)

Objective To study the effects of 5-HT1A receptor in hippocampal dentate gyrus on anxiety behavior of adult rats with Parkinson's disease (PD).Methods PD model in rats was constructed by using 6-hydroxydopamine (6-OHDA) to lesion the medial forebrain bundle (MFB).In lesioned rats and sham-operated rats,we examined the changes of the number of TFH-positive neurons in the SNc and VTA.We also examined how 5-HT1A receptor of hippocampal dentate gyrus (DG) modulates the anxiety-related behavior using open field test and elevated plus-maze test,and examined monoamine neurotransmitter changes in the striatum,medial prefrontal cortex (mPFC),amygdale and ventral hippocampus using reverse-phase high performance liquid chromatography with electrochemical detection.Results In 6-OHDA-lesioned rats,the SNc of the injected side showed a total loss of TH-ir neurons compared with the unlesioned side,and the number of TH-ir neurons in the VTA on the lesioned side decreased significantly to (34± 2)％.The locomotor activity in 6-OHDA-lesioned rats decreased compared with that in sham-operated rats.Unilaterally lesioning the MFB decreased the percentage of time spent in the center in the open-field test and percentages of open arm entries and open arm time of elevated plus maze test when compared with sham-operated rats.In the sham-operated rats,intra-DG injection of 8-OH-DPAT did not affect anxiety-like behavior.In 6-OHDA-lesioned rats,intra-DG injection of 8-OH-DPAT significantly increased the percentage of time spent in the center at a dose of 100 ng and 500 ng when compared with saline injection into the DG.Injection of WAY-100635 did not affect rats in the two groups.Unilateral 6-OHDA lesions of the MFB in rats significantly decreased DA levels in the ipsilateral striatum,mPFC,amygdale and ventral hippocampus compared with those in sham-operated rats,but had no effect on 5-HT or NA levels in those structures.Conclusion Unilateral 6-OHDA lesion of the MFB not only totally damaged DA neurons in SNc and partly damaged DA neurons in VTA,but also decreased DA level in the ipsilateral striatum,mPFC,amygdale and ventral hippocampus.Unilateral 6-OHDA lesion of the MFB induces anxiety-related behavior,and activation of 5-HT1A receptor in the hippocampal dentate gyrus has anxiolytic effect in the rat model of Parkinson's disease.

Chromosomal context and replication properties of ARS plasmids in Schizosaccharomyces pombe.

Short, specific DNA sequences called as Autonomously Replicating Sequence (ARS) elements function as plasmid as well as chromosomal replication origins in yeasts. As compared to ARSs, different chromosomal origins vary greatly in their efficiency and timing of replication probably due to their wider chromosomal context. The two Schizosaccharomyces pombe ARS elements, ars727 and ars2004, represent two extremities in their chromosomal origin activity – ars727 is inactive and late replicating, while ars2004 is a highly active, early-firing origin. To determine the effect of chromosomal context on the activity of these ARS elements, we have cloned them with their extended chromosomal context as well as in the context of each other in both orientations and analysed their replication efficiency by ARS and plasmid stability assays. We found that these ARS elements retain their origin activity in their extended/altered context. However, deletion of a 133-bp region of the previously reported ars727- associated late replication enforcing element (LRE) caused advancement in replication timing of the resulting plasmid. These results confirm the role of LRE in directing plasmid replication timing and suggest that the plasmid origin efficiency of ars2004 or ars727 remains unaltered by the extended chromosomal context.

Bioluminescent bioreporter for assessment of arsenic contamination in water samples of India.

In the present study the most efficient R-factor controlling the ars operon was selected after screening of 39 Escherichia coli isolates by minimum inhibitory concentration test (MIC) studies from water samples of different geographical locations of India. Among all, strain isolated from Hooghly River (West Bengal) was found to have maximum tolerance towards arsenic and was further used for the development of bioreporter bacteria. Cloning of the ars regulatory element along with operator-promotor and luxCDABE from Photobacteria into expression vector has been accomplished by following recombinant DNA protocols. The bioreporter sensor system developed in this study can measure the estimated range of 0.74–60 μg of As/L and is both specific and selective for sensing bioavailable As. The constructed bacterial biosensor was further used for the determination of arsenic ion concentration in different environmental samples of India.

Spectrophotometric studies using ion-pair formations of Ranitidine hydrochloride in pure and in Pharmaceutical forms with some dyestuff reagents.

Simple, rapid and sensitive spectrophotometric procedure is suggested for the determination of ranitidine hydrochloride (RNH) drug in pure form and in pharmaceutical formulations. The method was based on the ionpair formations of RNH with different dyestuff reagents such as methyl orange (MO), bromocrysol purple (BCP), eriochrome cyanine R (ECR) and alizaraine red S (ARS). The obtained ion-pairs were measured spectrophotometrically at 408, 420, 330 and 326 nm by using BCP, MO, ECR and ARS reagents, respectively. Beer’s plots were linear in the concentration range of 5-200, 20-350, 10-150 and 10-180 μg mL−1 RNH, with correlation coefficients not less than 0.9991, 0.9996, 0.9993 and 0.999 using BCP, MO, ECR and ARS reagents, respectively. The Sandell sensitivity was found to be 0.813, 0.462, 0.541and 0.630 μg cm−2 for BCP, MO, ECR and ARS, respectively. Standard deviation (SD = 0.024-0.028, 0.018-0.023, 0.016-0.021 and 0.023–0.029) and relative standard deviation (RSD% = 0.123-0.943, 0.0102-0.82, 0.118-0.145 and 0.132-0.178%) (n = 4) values using BCP, MO, ECR and ARS reagents, respectively, were obtained. These results were also confirmed with percent recovery of 99.78–100.52%, 99.86-101.12%, 99.82–100.31% and 100.18-101.25 % for BCP, MO, ECR and ARS reagents, respectively. This method was successfully applied for determination of RNH in aciloc tablet. The calculated t- and F- values (95% confidence limit) indicate no significant differences between the proposed and official methods.

Quantitative Electroencephalography Findings in Attention-Deficit Hyperactivity Disorder According to Korean DISC-IV / 신경정신의학

OBJECTIVES: This study was designed to investigate the clinical availability of quantitative electroencephalography (QEEG) as an auxiliary diagnostic tool in attention-deficit hyperactivity disorder (ADHD). METHODS: A total 95 participants completed examinations, which included Korean ADHD Rating Scale (K-ARS), Diagnostic Interview Schedule for Children Version IV-Korean Version (DISC-IV), and QEEG. From the result of the DISC-IV, we divided them into three groups, ADHD, ADHD NOS (not otherwise specified), and Normal control. The QEEG was analyzed by the ranges of Hz : delta (1-4 Hz), theta (4-8 Hz), alpha (8-12 Hz), beta (12-25 Hz), and high beta (25-30 Hz). RESULTS: ADHD NOS group showed significantly decreased delta activity (NOSnormal, p=0.044) compared with normal control. ADHD group showed increased high beta activity (ADHD>NOS, p=0.043) compared with NOS group. The z-scores of relative power of theta were negatively correlated with the K-ARS at O1, O2 electrodes of ADHD group. On the other hand, the z-scores of relative power of high beta were negatively correlated with the K-ARS at F7, F8 electrodes of ADHD NOS group. CONCLUSION: We confirmed QEEG abnormalities in ADHD patients, especially the difference between ADHD NOS and Normal control. Therefore, we expect to use QEEG as the valuable tool to diagnose ADHD accurately.

Anti-aminoacyl-tRNA synthetase antibody as an important predictor for interstitial lung disease in patients with idiopathic inflammatory myopathies / 中华风湿病学杂志

ObjectiveTo determine the serum level of anti-aminoacyl-tRNA synthetase (ARS)antibody in patients with polymyositis (PM) and dermatomyositis (DM) and to investigate the value of anti-ARS antibody for diagnosing interstitial lung diseases(ILD) in patients with PM/DM compared with anti-Jo-1 antibody.MethodsSerum anti-ARS antibody concentrations were measured by ELISA in 109 adult PM/DM patients,20 patients with SLE,20 patients with RA and 50 healthy controls.T test,Mann-Whitney U test,chi-square test or Fisher exact test were used to compare the sensitivity and specificity for diagnosing ILD in PM/DM patients between anti-ARS antibody and anti-Jo-1 antibody.Moreover,McNemar test was employed to analyze the correlation between the clinical features and anti-MDA5 antibody in PM/DM patients.Results The serum positive rate of anti-ARS antibody was 37.9％,7.8％,10％,0 and 0 in PM/DM patients with ILD and without ILD,patients with SLE and RA and healthy controls,respectively.Serum anti-ARS antibody levels and positive rate in the PM/DM patients with ILD were significantly higher when compared with PM/DM patients without ILD,patients with SLE and RA and healthy controls (X2=-13.5,5.45,10.57,15.17; P＜0.01 ).Anti-ARS antibody presented a significantly higher sensitivity for diagnosing ILD in patients with PM/DM compared to anti-Jo-1 antibody (P＜0.01).The rate of fever and ILD were significantly higher in anti-ARS positive group than anti-ARS negative group(X2=12.55,13.53; P＜0.05),while heliotrope rash and shawl sign occurred more often in anti-ARS negative group(X2=5.7,5.8; P＜0.05).Additionally,follow-up study showed that the serum anti-ARS antibody were all negative in nine patients who died of PM/DM with ILD (P＜0.05).ConclusionSerum anti-ARS antibody is a stronger predictor for early diagnosis of PM/DM with ILD compared to anti-Jo-1 antibody.The detection of anti-ARS antibody can be widely applied to clinical practice.

A colaboração na produção de artigos dos professores do Programa de Pós-Graduação em Ciência do Movimento Humano da ESEF, da UFRGS, entre 2007 e 2009: análise de rede social / Collaboration in production of articles of teachers of the Graduate Studies Program in Human Movement Science of the ESEF of the UFRGS between 2007 and 2009: social network analysis / La colaboración en la producción de artículos de los profesores del Programa de Posgraduación en Ciencia del Movimiento Humano de la ESEF de la UFRGS entre 2007 a 2009: análisis de red social

O pesquisador solitário é uma figura distante da contemporaneidade. O trabalho colaborativo na comunidade científica se expandiu enormemente no século XX apoiado pela tecnologia da informação. Para tal quadro contribuíram a utilização dos recursos eletrônicos e o acirramento da competitividade no meio acadêmico e em outras organizações. A colaboração em rede fortalece laços entre pesquisadores e instituições que, dessa forma, gerem os recursos e as atividades interdisciplinares de pesquisas e contribuem para o sustento mútuo na construção do saber científico. Este artigo visa contribuir para a identificação das redes de colaboração dos professores do Programa de Pós-Graduação em Ciência do Movimento Humano (PPGCMH) da UFRGS, no período de 2007 a 2009, referente ao último triênio de avaliação da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Foram identificados 204 artigos e 280 autores nos artigos publicados. Os resultados apontam no sentido de uma rede colaborativa em construção, com poucas ligações entre os professores e mais entre os grupos de pesquisa. Este artigo pretende contribuir para estudos no campo da produção científica da educação física, e servir como um instrumento às atividades de investigação e de possibilidades de intercâmbio institucional

The lone researcher is a figure distant from contemporaneity. Collaborative work in the scientific community expanded enormously in the twentieth century supported by information technology. The use of electronic resources and intense competition in the academic means and other organizations contributed to this outcome. Network collaboration strengthens ties between researchers and institutions, which thus generate resources and interdisciplinary research activities and contribute to mutual support in the construction of scientific knowledge. This article aims to contribute to the identification of collaborative networks of teachers in the Graduate Studies Program in Human Movement Science (PPGCMH) of the UFRGS, in the period 2007-2009, covering the last triennial valuation of the Coordination of Personnel Training (CAPES). 204 articles and 280 authors in published articles were identified. The results point towards a collaborative network under construction, with few links between teachers and more among research groups. This article contributes to studies in the scientific production field of physical education, and as a tool to research activities and opportunities for institutional exchange

El investigador solitario es una figura distante de la contemporaneidad. El trabajo colaborativo en la comunidad científica se expandió enormemente en el siglo XX apoyado por la tecnología de la información. Para este cuadro contribuyeron la utilización de los recursos electrónicos y la agudización de la competitividad en el medio académico y en otras organizaciones. La colaboración en red fortalece lazos entre investigadores e instituciones, que, de esta forma, generan los recursos y las actividades interdisciplinarias de investigación y contribuyen al sustento mutuo en la construcción del saber científico. Este artículo busca contribuir a la identificación de las redes de colaboración de los profesores del Programa de Posgraduación en Ciencia del Movimiento Humano (PPGCMH) de la UFRGS, en el periodo de 2007 a 2009, referente al último trienio de evaluación de la Coordinación del Perfeccionamiento del Personal de Nivel Superior (CAPES, en su sigla en portugués). Fueron identificados 204 artículos y 280 autores en los artículos publicados. Los resultados apuntan en el sentido de una red colaborativa en construcción, con pocas conexiones entre los profesores y más entre los grupos de investigación. Este artículo pretende contribuir para estudios en el campo de la producción científica de la educación física, y como un instrumento a las actividades de investigación y de posibilidades de intercambio institucional

Diagnostic value of (1,3)-β-D-glucan assay and galactomannan test for invasive fungal infection in patients of acute radiation sickness / 中华放射医学与防护杂志

Objective To investigate the diagnostic values of (1,3)-β-D-glucan (G) and galactomannan (GM) for invasive fungal infection (IFI) in patients of acute radiation sickness (ARS).Methods Samples of periogeral blood,pharyngeal secretion,urine,and feces were collected from 316patients with ARS and suspected to suffer from IFI,192 males and 124 females,aged 60.50(1-96),with the underlying diseases of blood or respiration systems.Platelia Aspergillus EIA kit was used to detect the plasma BG (G test),and ELISA was used to detect the serum GM (GM test).Fungal culture and bacterial culture were performed.Results The positive rates of G test,GM test,and fungal culture were 36.33%,35.84% ,and 34.18% respectively,but the positive rate of fungal culture of blood sample was 1/316 only.Pearson correlation analysis showed that G test,GM test and fungal culture test were positively correlated with IFI clinical diagnosis respectively (x2 = 0.564,0.357,0.727,P ＜ 0.05).Conclusions Easy to operate,rapid,and highly sencitive,G test and GM test can be used as adjunctive methods for early IFI diagnosis in ARS patients.

Case-Control Association Study of the Norepinephrine Transporter Gene Polymorphism in Children with ADHD

OBJECTIVES: This study aimed to examine the association between norepinephrine transporter gene (SLC6A2) polymorphisms and attention-deficit hyperactivity disorder (ADHD) and to examine the relationship between the genotypes and allele variants of SLC6A2 and results of the Korean version of the parent ADHD rating scale (K-ARS). METHODS: We examined the association between ADHD and norepinephrine transporter gene polymorphism using DNA from 137 Korean children with ADHD and 120 normal controls. We compared the genotype distributions and allele frequencies of SLC6A2 polymorphism between the control group and the ADHD group. Then, we correlated the children's K-ARS mean totals, inattention scores, and hyperactivity/impulsivity scores with the genotypes and alleles for each SLC6A2 polymorphism. RESULTS: There were no significant differences in genotype and allele distribution for each SLC6A2 polymorphism, as shown by the Chi-square test (p>.01). There was a trend toward a difference in allele frequency in rs 5568, but it was not statistically significant after adjusting for multiple comparisons (p=.048). Also, there were no significant differences in K-ARS scores according to the genotypes and alleles for the SLC6A2 polymorphisms. CONCLUSION: Our study found no significant evidence of an association between SLC6A2 polymorphisms and ADHD.

Impacto de dos arrecifes artificiales en la pesca artesanal diurna del Golfo de Morrosquillo, Caribe de Colombia

Impact of two artificial reefs on diurnal artisanal fishing at gulf of Morrosquillo, Colombian Caribbean. Fishing activity in two artificial reefs (ARs) was monitored between August 2001 and August 2002 in the gulf of Morrosquillo, to investigate their possible impact on the fishery dynamics. We determined catch per unit effort (CPUE) and catch composition, and found 19 species in Punta de Piedra and 36 in Tolú (16 species common to both). In Punta de Piedra, CPUE was 0.40 kg/hour/fisherman and estimated annual catch was 1 830 kg (Tolú values: 0.42 kg/hour/fisherman and 4 820 kg). No significant differences in CPUE were found between ARs and no seasonal pattern was evident. The differences in total composition and extracted biomass between ARs were mainly a consequence of greater effort exerted in Tolú, whereas the absence of trends and correlation with environmental factors of CPUE and species caught suggests that the ARs are near their carrying capacity. As expected, the ARs supported and improved CPUE and fishing success in comparison with reported values of natural habitats, traditional fishing grounds and some artificial habitats in the zone, owing to their greater volume, structural complexity and vertical profile. However, this increase could also be the result of biomass aggregation, not production. The potential negative impacts of ARs on the environment require experimental research. The deployment of non-extractive ARs and the controlled usage for fishery purposes could be a useful tool for resource enhancement and management in the area. Rev. Biol. Trop. 57 (4): 993-1007. Epub 2009 December 01.

Entre los meses de agosto de 2001 y agosto de 2002 se hizo el seguimiento de la actividad pesquera en dos arrecifes artificiales (AAs) instalados en el Golfo de Morrosquillo, con el fin de evaluar sus posibles impactos en la dinámica pesquera del área. Se determinó la captura por unidad de esfuerzo (CPUE) y su composición, registrando un total de 19 especies en Punta de Piedra y 36 en Tolú con 16 especies en común. La CPUE fue de 0.40kg/hora/ pescador en Punta de Piedra y de 0.42kg/hora/pescador en Tolú, y la biomasa extraída total estimada fue de 1 830kg y 4 820kg en Punta de Piedra y Tolú respectivamente, para pescadores que trabajaron con línea de mano en horas diurnas. No se encontraron diferencias significativas de CPUE entre los AAs y no fue evidente ningún patrón estacional. Las diferencias en composición y biomasa extraída total entre los AAs fueron principalmente consecuencia del mayor esfuerzo ejercido en Tolú, mientras que la ausencia de tendencias y correlaciones de los factores abióticos con la CPUE y las especies capturadas, sugiere que los AAs están cerca de su capacidad de carga. Como era de esperarse, los AAs mantuvieron y mejoraron la CPUE en comparación con valores reportados en hábitats naturales, caladeros tradicionales de pesca y algunos hábitats artificiales del sector, debido a su mayor volumen, complejidad estructural y perfil vertical. Sin embargo, este incremento puede ser el resultado de la agregación de biomasa más que la producción en los AAs, lo cual tiene impactos negativos potenciales sobre el ambiente que necesitan ser evaluados con el desarrollo de investigaciones experimentales. La disposición de AAs no-extractivos y el uso controlado para propuestas pesqueras, puede ser una herramienta útil para el enriquecimiento y manejo de los recursos en el área.

Impact of Adenotonsillar Hypertrophy on Sleep and Attention Deficit-Hyperactivity Symptoms in Children / 신경정신의학

OBJECTIVES : Adenotonsillar hypertrophy is one of the principal causes associated with snoring, sleep apnea, and restless sleep. These sleep-disordered breathing (SDB) is associated with hyperactivity, rebellious behavior, aggressiveness, enuresis, inattention, social withdrawal, and learning difficulties. The purpose of this study was to assess the impact of adenotonsillar hypertrophy on attention deficit hyperactivity symptoms and sleep-related symptoms in children by standard tests. METHODS : Children aged 5 through 15 years old, a total of 65 who were scheduled for Tonsillectomy and Adenoidectomy, were studied. The control group consisted of 30 healthy children of the same age. In both groups, attention-deficit hyperactivity dis-order (ADHD) was evaluated by Kiddie-Schedule for Affective Disorders and Schizophrenia-Present and Lifetime Version (K-SADS-PL). The degree of inattention, hyperactivity and sleep-related symptoms was evaluated by Korean ADHD Rating Scales (K-ARS), ADHD diagnostic system (ADS) and pediatric sleep questionnaire. The K-ARS and sleep questionnaire were completed by the parents of the children. RESULTS : Acording to K-SADS-PL, 23 subjects out of 65 subjects in the patient group and 2 subjects out of 30 subjects in the control group were diagnosed with ADHD. According to sleep questionnaires, the patient group reported significantly more problems than the control group. The patients had significantly higher cumulative questionnaire scores than the control group. In linear regression analysis, the cumulative score of sleep questionnaire was associated with inattention subscale scores, hyperactivity/impulsivity subscale scores and the total score of K-ARS, and also with the response time and the standard deviation of response time of ADS. In relation to ADHD, the patient group had significantly higher ADHD scores in K-ARS inattention subscale and significantly slower response time in ADS compared to the control group. Comparing SDB children without ADHD and the control group without ADHD by excluding ADHD children in both groups, SDB children without ADHD did not show significantly higher scores in K-ARS but did show significantly slower ADS response time. CONCLUSION : Children with adenotonsillar hypertrophy are associated with increased SDB and ADHD symptoms. Therefore they may need intensive treatment such as surgical intervention.

Impact of Adenotonsillar Hypertrophy on Sleep and Attention Deficit-Hyperactivity Symptoms in Children / 신경정신의학

OBJECTIVES : Adenotonsillar hypertrophy is one of the principal causes associated with snoring, sleep apnea, and restless sleep. These sleep-disordered breathing (SDB) is associated with hyperactivity, rebellious behavior, aggressiveness, enuresis, inattention, social withdrawal, and learning difficulties. The purpose of this study was to assess the impact of adenotonsillar hypertrophy on attention deficit hyperactivity symptoms and sleep-related symptoms in children by standard tests. METHODS : Children aged 5 through 15 years old, a total of 65 who were scheduled for Tonsillectomy and Adenoidectomy, were studied. The control group consisted of 30 healthy children of the same age. In both groups, attention-deficit hyperactivity dis-order (ADHD) was evaluated by Kiddie-Schedule for Affective Disorders and Schizophrenia-Present and Lifetime Version (K-SADS-PL). The degree of inattention, hyperactivity and sleep-related symptoms was evaluated by Korean ADHD Rating Scales (K-ARS), ADHD diagnostic system (ADS) and pediatric sleep questionnaire. The K-ARS and sleep questionnaire were completed by the parents of the children. RESULTS : Acording to K-SADS-PL, 23 subjects out of 65 subjects in the patient group and 2 subjects out of 30 subjects in the control group were diagnosed with ADHD. According to sleep questionnaires, the patient group reported significantly more problems than the control group. The patients had significantly higher cumulative questionnaire scores than the control group. In linear regression analysis, the cumulative score of sleep questionnaire was associated with inattention subscale scores, hyperactivity/impulsivity subscale scores and the total score of K-ARS, and also with the response time and the standard deviation of response time of ADS. In relation to ADHD, the patient group had significantly higher ADHD scores in K-ARS inattention subscale and significantly slower response time in ADS compared to the control group. Comparing SDB children without ADHD and the control group without ADHD by excluding ADHD children in both groups, SDB children without ADHD did not show significantly higher scores in K-ARS but did show significantly slower ADS response time. CONCLUSION : Children with adenotonsillar hypertrophy are associated with increased SDB and ADHD symptoms. Therefore they may need intensive treatment such as surgical intervention.

Screening for Attention Deficit/Hyperactivity Disorder for Children in Seoul / 신경정신의학

OBJECTIVES: The purpose of this study is to investigate a cut-off value for screening children with ADHD in community based sample using the Korean Child Behavior Checklist (K-CBCL) and the Korean ADHD Rating Scale (K-ARS). METHODS: Children aged 6 to 8 (n=661) participated in this study. The K-CBCL and the K-ARS were used as the screening instruments. Diagnoses were confirmed through the Diagnostic Interview Schedule for Children Version IV (DISC-IV). RESULTS: Of the 189 subjects who underwent the DISC-IV, 26 subjects were diagnosed as ADHD. In the K-CBCL, scores in the attention problems, externalizing problems and total behavioral problems were positively correlated with the total score of the K-ARS. The K-ARS-Parent version (K-ARS-P) and above three scales in the K-CBCL showed significant screening validity in the ROC curve analysis. The reasonable level of sensitivity, specificity, and negative predictive value were obtained in the total scores > or =90th percentile in the K-ARS-Parent and > or =60T scores in the K-CBCL attention problems. The optimal level of specificity, positive predictive value and negative predictive value were obtained when combined the K-CBCL (> or =60T scores in the attention problems and > or =63T scores in the total problems) and the total scores > or =90th percentile in the K-ARS-P. CONCLUSION: The combined use of the K-CBCL and the K-ARS-P may serve as useful screening methods for ADHD children.

Normative Study of the K-ARS(Korean ADHD Rating Scale) for Parents

OBJECTIVES: The K-ARS(Korean ADHD Rating Scale) is one of the most important assessment tool of attentiondeficit hyperactivity disorder(ADHD) in Korea. In this study, we presented detailed normative data on the K-ARS for school-aged children in Seoul metropolitan area to put it to practical use. METHODS: The subjects were 2,397 students(1,223 boys and 1,174 girls, aged 6-12) from 4 elementary schools in Seoul, and one caretaker of each child completed the K-ARS for parents. Children who showed high scores of the KARS for parents were screened, and 2 child psychiatrists interviewed them to make a clinical diagnosis. We compared the mean scores of the K-ARS for parents between ADHD and normal group, and examined the percentage of correct classification. RESULTS: There were some differences in score of the K-ARS for parents according to sex and age, so we presented continuous normative data with T score and subdivided cut-off points for ADHD screening. Interviews with child psychiatrists using DSM-IV criteria were performed to test diagnostic validity, and the difference in every the K-ARS for parents index between ADHD and normal group was significant(p<.001). Using 3 different cut-off points(80th, 90th, 93rd percentage), the accuracies of ADHD correct classification were 67.9, 72.2, 71.1% and all 3 canonical discriminants were significant(p<.05) between ADHD and normal group. CONCLUSION: The normative data and cut-off points on the K-ARS for parents are useful in screening ADHD children in Seoul metropolitan area.