ABSTRACT
OBJECTIVE@#To assess the effect of early abdominal puncture drainage (APD) on autophagy and Nrf-2/HO-1 pathway in rats with severe acute pancreatitis (SAP) and explore the possibile mechanism.@*METHODS@#Thirty-two male SD rats were randomly divided into sham-operated (SO) group, SAP group with retrograde injection of 4% sodium taurocholate, APD group with insertion of a drainage tube into the lower right abdomen after SAP induction, and APD + ZnPP group with intraperitoneal injection of 30 mg/kg ZnPP 12 h before APD modeling. Blood samples were collected from the rats 12 h after modeling for analysis of amylase and lipase levels and serum inflammatory factors. The pathological changes of the pancreatic tissue were observed with HE staining. Oxidative stress in the pancreatic tissue was detected with colorimetry, and sub-organelle structure and autophagy in pancreatic acinar cells were observed by transmission electron microscopy. The expressions of autophagy-related proteins and Nrf-2/HO-1 pathway were detected using RT-PCR and Western blotting.@*RESULTS@#Compared with those in SAP group, the rats with APD treatment showed significantly alleviated pathologies in the pancreas, reduced serum levels of lipase, amylase and inflammatory factors, lowered levels of oxidative stress, and activated expressions of Nrf-2/HO-1 pathway in the pancreas. The ameliorating effect of ADP was significantly inhibited by ZnPP treatment before modeling. APD obviously reversed mitochondrial and endoplasmic reticulum damages and p62 accumulation induced by SAP.@*CONCLUSION@#APD treatment can suppress oxidative stress and repair impaired autophagy in rats with SAP by activating the Nrf-2/HO-1 pathway, thereby reducing the severity of SAP.
Subject(s)
Animals , Male , Rats , Acute Disease , Amylases/blood , Autophagy , Drainage , Heme Oxygenase (Decyclizing) , Lipase/blood , NF-E2-Related Factor 2 , Oxidative Stress , Pancreas/pathology , Pancreatitis/surgery , Punctures , Rats, Sprague-DawleyABSTRACT
El conocimiento de la vascularización de la pared anterior del abdomen es un importante instrumento para la realización de incisiones y punciones abdominales, contribuyendo a evitar algunas complicaciones reurrentes de este procedimiento. Con el propósito de estudiar la emergencia y el trayecto de las arterias epigástricas superiores, fueron disecados 32 cadáveres adultos, del sexo masculino, no fijados, blancos y no blancos, con edades entre 18 y 65 años. Se observó el trayecto de las arterias epigástricas superiores, su emergencia y su forma de distribución. Las arterias epigástricas superiores emergieron por atrás del 7 cartílago costal en todos los casos. En el 75% de los casos las arterias epigástricas se presentaron como un tronco único y en 15 casos (23,44%) bifurcadas, siendo 11 a la derecha y 4 a la izquierda y en 1 caso (1,56%) a la izquierda. En este último, caso dio origen a tres ramos principales. Cuando era un tronco único, su trayecto fue descendente de medial para lateral en relación al margen lateral del músculo recto del abdomen. Cuando era bifurcada, sus ramos se separaron uno del otro luego de su emergencia, volviendo a convergir próximo a la cicatriz umbilical.
The knowledge of the vascularization of the anterior abdominal wall is very important on the incisions and abdominal punctures, contributing to avoid the injuries of this vessels during this procedures. With the goal to study the emergency and the trajectory of the superior epigastric arteries, 32 adult not preserved cadavers, of the masculine sex, white and not white, with age between 18 and 65 years were studied. The trajectory of the superior epigastric arteries, your emergency and distribution form were observed. The superior epigástric arteries emerged behind to 7th costal cartilage in all the cases. In 75% of the cases they appeared like an only trunk and in 15 cases (23,44%) they appeared forked, being 11 to the right and 04 to the left and in 01 case (1,56%) to the left, it'd origin three main branches. When in an only trunk, your trajectory went descending of medial for lateral in relationship to the lateral margin of the rectus abdominal muscle. When forked, your branches separated one of the other immediatelly after your emergency, back converging close to the umbilical scar.
Subject(s)
Humans , Male , Adolescent , Adult , Middle Aged , Aged , Punctures , Epigastric Arteries/anatomy & histology , Abdominal Wall/blood supply , CadaverABSTRACT
Objective To introduce a new method that abdominal puncture is used in ventriculoperitoneal shunt. Methods A retrospective review of the clinical materials of 49 patients who underwent improving ventriculoperitoneal shunt.Results After operations, all the patients had fellow-up survey that the rates of infection and obstruction are both 4.08%. They are lower than that of the lectures. Conclusion It has many advantages that abdominal puncture taken the place of incision of abdominal cavity. They are less wounded, less infection, less abdominal complication , not easy to be packed by the omentum and so on.