ABSTRACT
OBJECTIVE: To investigate the hygroscopicity of vitamin B12 and the related problems of its chemical reference substance (CRS) that should be paid attention to. METHODS: UV absorption coefficient was determined at 361 nm, using water as solvent. Dynamic vapor absorption analysis (DVS) was applied to evaluate the moisture sorption trend and capacities of vitamin B12 under different humilities. RESULTS: Owing to the strong hygroscopicity of vitamin B12, deviation in the process of weighing might result in smaller UV absorption coefficient compared with the actual value, especially for dried and low moisture content raw materials in high-humidity environment. CONCLUSION: In the study of drug quality control for vitamin B12, the strong hygroscopicity of the raw material should be paid attention to, particularly in the research and application of vitamin B12 CRS.
ABSTRACT
Objective: To confirm the hepatotoxic Q-marker of Euodiae Fructus decoction. Methods: The hepatotoxicity of target compounds was studied by hepatotoxicity index and flow cytometry. The targets and target organs were predicted by network pharmacology. The qualitative absorption analysis of 3-O-trans-caffeoylgluconic acid (1), 4-O-trans-caffeoylgluconic acid (2), 2-O-trans-caffeoylgluconic acid (3) was performed by LC-MS. Through the systematic study of Q-marker properties, the confirmation study of hepatotoxicity Q-marker in Euodiae Fructus decoction was performed. Results: Compounds 1, 2, and 3 inhibited the growth of L02 cells in G0/G1 phase and had direct toxicity to L02 cells, which led to hepatotoxicity. The target organ may be the liver by the network pharmacology analysis. After drug administration, compounds 1, 2, and 3 could be absorbed into the blood by prototype. Compounds 1, 2, and 3 had properties of structural confirmation, toxicity confirmation, measurability, and transmissibility. Conclusion: Compounds 1, 2, and 3 could be confirmed as hepatotoxic Q-marker of Euodiae Fructus decoction.