ABSTRACT
The present study was designed to characterize the polyphenols isolated from Acacia mearnsii bark crude extract (B) and fractions (B1-B7) obtained by high-speed counter-current chromatography (HSCCC) and evaluate their anti-inflammatory and carbolytic enzymes (α-glucosidase and α-amylase) inhibitory activities. Fractions B4, B5, B6, B7 (total phenolics 850.3, 983.0, 843.9, and 572.5 mg·g, respectively; proanthocyanidins 75.7, 90.5, 95.0, and 44.8 mg·g, respectively) showed significant activities against reactive oxygen species (ROS), nitric oxide (NO) production, and expression of pro-inflammatory genes interleukin-1β (IL-1β) and inducible nitric oxide synthase (iNOS) in a lipopolysaccharide (LPS)-stimulated mouse macrophage cell line RAW 264.7. All the extracts suppressed α-glucosidase and α-amylase activities, two primary enzymes responsible for carbohydrate digestion. A. mearnsii bark samples possessed significantly stronger inhibitory effects against α-glucosidase enzyme (IC of 0.4-1.4 μg·mL) than the pharmaceutical acarbose (IC 141.8 μg·mL). B6 and B7 (IC 17.6 and 11.7 μg·mL, respectively) exhibited α-amylase inhibitory activity as efficacious as acarbose (IC 15.4 μg·mL). Moreover, B extract, at 25 µg·mL, significantly decreased the non-mitochondrial oxidative burst that is often associated with inflammatory response in human monocytic macrophages.
Subject(s)
Animals , Mice , Acacia , Chemistry , Anti-Inflammatory Agents , Pharmacology , Carbohydrate Metabolism , Glycoside Hydrolase Inhibitors , Pharmacology , Inflammation , Metabolism , Interleukin-1beta , Metabolism , Lipopolysaccharides , Macrophages , Nitric Oxide , Metabolism , Nitric Oxide Synthase Type II , Metabolism , Plant Bark , Chemistry , Plant Extracts , Chemistry , Pharmacology , Polyphenols , Pharmacology , Proanthocyanidins , Pharmacology , alpha-Amylases , alpha-Glucosidases , MetabolismABSTRACT
ABSTRACT: In the present study, tannin extracts (rinsed or not) were compared to the use of sodium hypochlorite and tap water on fresh cut crisp leaf lettuce stored under modified atmosphere packaging. Effects of these sanitizers on total color difference and microbial levels of the product after sanitization and storage for 9 days at 3°C were evaluated. Performance of rinsed SM(r) tannin extract was comparable to the results of chlorine solution for all the analyzed parameters and; furthermore, that extract presented a high reduction in the initial bacterial count of minimally processed lettuce. However, storage of tannin extracts, did not impart better outcomes than the use of tap water. Therefore, the tannin extract storage SM(r) could be used in washing water to reduce the initial microbiological load, avoiding cross contamination in vegetables minimally processed.
RESUMO: Extratos de taninos (com e sem enxague) foram comparados com o uso de hipoclorito de sódio e água em alface crespa minimamente processada, sob atmosfera modificada. Foram avaliadas diferença total de cor e análises microbiológicas após a sanitização e durante o armazenamento dos produtos (9 dias à 3°C). O extrato de tanino SM(r) com enxague apresentou resultados semelhantes aos da solução de cloro para todos os parâmetros analisados e, além disso, apresentou uma elevada redução da contagem inicial microbiológica de alface minimamente processada. No entanto, durante o armazenamento, SM(r) não apresentou melhores resultados do que o uso de água. Portanto, SM(r) poderia ser utilizado na desinfecção da água de lavagem das alfaces, reduzindo a microbiota inicial e, assim, impedir a contaminação cruzada durante o processamento mínimo de vegetais.
ABSTRACT
Fine root length density (FRLD) was evaluated in mixed and monospecific stands of Eucalyptus grandis x E. urophylla and Acacia mearnsii in Southern Brazil. FRLD (≤2,0mm) at 8 and 18 months after planting in the treatments: 100E (100% of eucalyptus); 100A (100% of Acacia mearnsii); 50E:50A (50% of eucalyptus + 50% of Acacia mearnsii). The findings demonstrated that the FRLD at 8 months of age have the same distribution, in the two different species, in the distribution of the different soil layers, reaching the maximum projection of 125cm from the tree trunk. For the age of 18 months after planting, it was verified that the FRLD in the monospecific stand of Acacia mearnsii was higher than in the monoculture and mixed stand of Eucalyptus grandis x E. urophylla. Therefore, no interaction, neither positive nor negative, between the root systems of Eucalyptus grandis x E. urophylla and Acacia mearnsii during the 18 months after planting was found. The higher FRLD is found at the soil layers surface, next to the tree trunk and in the planting line, followed by the diagonal and planting rows. The initial growth in length of the root system of Acacia mearnsii is more dynamic with higher density than the eucalyptus, but without interfering directly in the global growth of fine roots in mixed stands.
Avaliou-se a densidade do comprimento de raízes finas (DCRF) de plantios monoespecíficos e misto de Eucalyptus grandis x E. urophylla e de Acacia mearnsii na região sul do Brasil. A DCRF (≤2,0mm) foi determinada aos 8 e 18 meses após o plantio nos tratamentos: 100E (100% de eucalipto); 100A (100% de Acacia mearnsii); 50E:50A (50% de eucalipto + 50% de Acacia mearnsii). A DCRF aos oito meses de idade possui o mesmo comportamento para a ocupação das diferentes camadas do solo, atingindo uma projeção máxima de 125cm de distância em relação ao tronco da árvore. Já, aos 18 meses após o plantio, verificou-se que, no cultivo monoespecífico de Acacia mearnsii, a DCRF foi superior em relação ao monocultivo e plantio misto de Eucalyptus grandis x E. urophylla. Não ocorreram interações positivas ou negativas entre os sistemas radiculares do eucalipto e da acácia-negra durante os primeiros 18 meses após o plantio. A maior DCRF encontra-se nas camadas superficiais do solo, nas proximidades do tronco da árvore e na linha de plantio, seguida pela diagonal e entrelinha de plantio. O crescimento inicial em comprimento do sistema radicular da Acacia mearnsii é mais dinâmico e maior do que a do eucalipto, mas sem interferir diretamente no crescimento global das raízes finas no cultivo misto.