ABSTRACT
Objective To study and optimize the extraction technology of polysaccharides from Acanthopanax senticosus (AS) waste residue by response surface methodology (RSM). Methods Phenol-sulfuric acid method was used to quantitatively analyze polysaccharides from AS waste residue. Based on single factor, the extraction process factors of polysaccharides from AS waste residue were optimized by RSM. Results The best conditions for the response surface optimization were as follows: extraction temperature was 80 ℃, extracting time was 1.5 h, and liquid-material ratio was 21. The extraction rate of polysaccharide was 10.14% under the optimal conditions. Conclusion The extraction conditions of the polysaccharide from AS are optimized by response surface method. This data can provide a theoretical basis for the further exploitation and utilization of the subsequent waste residue of AS.
ABSTRACT
Objective: To studies the antimicrobial activity and stability of fermentation extracts produced by endophytic actinomycete strain 13-85 from Acanthopanax senticosus; Secondary metabolite biosynthesis genes were detected. Meanwhile, the strain was identified. Methods: Paper disc diffusion method was used to test the antimicrobial activity and stability of strain 13-85, the biosynthesis genes (PKS I, PKS II, NRPS, Halo, and CYP) were obtained by PCR, and sequences were analyzed. The strain was identified by phenotypic characteristics and molecular characteristics. Results: The fermentation broth of the strain showed broad-spectrum, strong activity, and stability against pathogenic microbes. The strain contains PKS II gene and NRPS gene. The PKS II sequence had 89% homology compared with Streptomyces fradiae PKS gene (Accession Number: AFO70128.1). The NRPS gene sequence had 95% homology with S. virginiae NRPS gene (Accession Number: WP_033225509.1). The sequence of 16S rRNA of the strain had 99.85% homology with S. amritsarensis (Accession Number: GQ906975). Conclusion: Metabolites of the actinomycete strain 13-85 with broad-spectrum antibacterial activity and better stability, have a value for development and application. The strain 13-85 was identified as Streptomyces amritsarensis, a valid species of genus Streptomyces.
ABSTRACT
Objective: To study the serum pharmacochemistry of leaves of Acanthopanax senticosus. Methods: Rats were ig administered A. senticosus extracts and then the serum was colleted. Rapid identification of transitional constituents absorbed into blood was carried out by UPLC-Q-TOF-MS/MS. The transitional constituents were identified and speculated by Peakview and MetabolitePilot data processing software using retention time, exact relative molecular mass, and cleavage fragments of MS/MS as indexes. Results: After administration of the leaves of A. Senticosus, 19 transitional constituents absorbed into blood were detected in serum, nine of them were prototype constituents and the other 10 were metabolites. Conclusion: Transitional constituents of the leaves of A. senticosus absorbed into blood are preliminarily identified, which could clarify its pharmacodynamic material basis.
ABSTRACT
Objective: To study the chemical constituents of Acanthopanax senticosus. Methods: The compounds were separated and purified by column chromatography and their structures were identified by spectroscopic methods. Results: Twelve compounds were isolated from the chloroform and n-butanol extract of A. senticosus, and their structures were determined as ciwujiatone (1), eleutheroside B1 (2), tachioside (3), alimoxide (4), erythro-1-(4-hydroxy-3-methoxy-phenyl)-2-{4-[(E)-3-hydroxy-1-propenyl] -2-methoxy-phenoxy}-1,3-propanediol (5), erythro-1,2-bis(4-hydroxy-3- methoxyphenyl)-1,3-propanediol (6), tortoside A (7), episyringaresinol 4′-O-β-D-glucopyranoside (8), (+)-lyoniresinol (9), benzyl-O-α-L-rhamno-pyranosyl (1→6)-β-D-glucopyranoside (10), 2,6-dimethoxy-4-(3-hydroxy-propen-1-yl) phenyl-4-O-α-L-rhamno-pyranosyl- (1→6)-β-D-glucopyranoside (11), and nicotinamide (12). Conclusion: Compounds 3-5 and 10-12 are isolated from A. senticosus for the first time.