ABSTRACT
OBJECTIVE: To develop an high-performance liquid chromatography (HPLC) method with diode array detector (DAD) for determination of acarbose in acarbose tablets. METHODS: An Alltima C18 column (4.6 mm×250 mm, 5 μm) was used for the separation, with acetonitrile-10 mmol·L-1 ammonium dihydrogen phosphate (containing 0.04% sodium 1-octanesulfonate, adjusted pH to 3.3 with H3PO4) (15∶85) as the mobile phase at the flow rate of 0.8 mL·min-1. The detection wavelength was set at 200 nm and the column temperature was maintained at 30℃. RESULTS: The method showed good linearity with a correlation coefficients (r) of 1.000 0. The specificity study demonstrated satisfactory resolutions between acarbose, other ingredients in the drug and forced degradation products. The precision and stability were satisfactory with the relative standard deviations (RSDs) of 0.088% and 0.22%, respectively. The average spiked recovery was 98.49% with RSD of 0.24% (n=9). The content of acarbose in four batches of acarbose tablets was 51.69, 51.75, 51.72 and 51.62 mg·tablet-1, respectively. CONCLUSION: The established method is accurate, simple and rapid, and can be utilized for determination of acarbose in acarbose tablets.
ABSTRACT
Objective To discuss the efficacy and safety of insulin aspart 30 injection combined with Akabo Tang (Acarbose tablets)about treatment of patients with type 2 diabetes.Methods 36 cases of type 2 diabetes were selected,by means of insulin aspart 30 combined acarbose tablets treatment,the levels of fasting plasma glucose (FPG)and glycosylated hemoglobin(HbA1c)before and after treatment were observed.Results The levels of fasting plasma glucose were (10.67 ±2.00)mmol/L and (6.76 ±0.47)mmol/L before and after treatment;The levels of HbA1c were (9.54 ±1.90)% and (7.70 ±0.82)% before and after treatment.After treatment,blood glucose and glycated hemoglobin levels in patients were significantly decreased (t =31.920,P <0.05;t =29.624,P <0.05). Conclusion The treatment that insulin aspart 30 injection combines acarbose tablets for type 2 diabetes patients is a safe,effective and convenient option.