ABSTRACT
Objective: To study the chemical constituents from Fritillaria pallidiflora. Methods: The constituents were isolated from F. pallidiflora and purified by column chromatography, and the structures were identified by spectra analysis and chemical methods. Results: Sixteen compounds were isolated from F. pallidiflora, including phenolic acids, esters, alkaloids, and the structures were identified as 1,4-diphenylbutane (1), cis-cinnamic acid (2), 4-hydroxy-3-methoxybenzaldehyde (3), acetovanillone (4), 9-octadecenoicacidmethylester (5), methyl ferulate (6), 1-O-feruloylglycerol (7), trans-isoferulic acid (8), syringaresinol (9), pinoresinol (10), 2,3-O-diferuloylglycerol (11), 1,3-O-diferuloyl-glycerol (12), cyclo (L-Pro-L-Ala) (13), cyclo (L-Leu-L-Val) (14), bis (diethylene glycol)phthalate (15), and cyclo-(Phe-Val) (16). Conclusion: All compounds are isolated from F. pallidiflora for the first time, and componds 1, 3-8, 10-13, 15, and 16 are isolated from the genus of Fritillaria for the first time.
ABSTRACT
Objective: To study the chemical constituents of Anoectochilus roxburghii. Methods: The chemical constituents of A. roxburghii were separated and purified by silica gel column chromatography, recrystallization, macroreticular resin, polyamide resin, ODS C18, and HPLC. Compound structures were identified by the spectral data and the physical and chemical properties. Results: From this plant, nineteen compounds were obtained, such as (22E, 24ξ)-24-isopropenyl-22-dehydrocholesterol (1), anoectosterol (2), stigmasterol (3), β-sitosterol (4), hircinol (5), moscatin (6), 5, 3'-dyhydroxy-3, 7, 4'-trimethoxyflavone (7), linoleic acid (8), 8, 9-epoxyheptadeca-1, 11, 14-triene (9), acetovanillone (10), parahydroxybenzaldehyde (11), parahydroxyacetophenone (12), 5-hydroxy-3', 4', 7-trimethoxyflavonol-3-O-β-D-rutinoside (13), isorhamnetin-3-O-robinobioside (14), β-adenosine (15), isorhamnetin-3-O-β-gentiobioside (16), loroglossin (17), corchoinoside C (18), and parahydroxybenzylalcohol (19). Conclusion: Compounds 1, 16, and 19 are isolated from Orchidaceae for the first time; Compounds 5, 6, 7, 10, and 12 are isolated from Anoectochilus Bl. for the first time; Compounds 9, 15, and 18 are isolated from this plant for the first time.
ABSTRACT
Objective: To study the antipyretic and anti-inflammatory constituents from the active fraction of Reduning Injection (RI). Methods: The active fraction of RI was screened by the LPS-induced mouse endotoxin shock model. The chemical constituents were isolated by chromatography on silica gel, ODS, Sephadex LH-20, Toyopearl HW-40 columns, reverse phase MPLC, and HPLC repeatedly, and their structures were identified by spectral data and physicochemical property. Results: The 95% ethanol eluate of RI on the macroporous adsorption resin column was proved to be the antipyretic and anti-inflammatory active fraction of RI. Fourteen compounds were isolated and identified as dibutyl phthalate (1), isovanillic acid (2), acetovanillone (3), phenylpropionic acid (4), geniposide (5), jasmigeniposide B (6), geniposidic acid (7), genipin-1-β-D-gentiobioside (8), 6″-O-trans-p-coumaroylgenipin gentiobioside (9), 6″-O-trans-feruloylgenipin gentiobioside (10), 6″-O-trans-sinapoylgenipin gentiobioside (11), jasmigeniposide A (12), 6″-O-trans-cinnamoylgenipin gentiobioside (13), and 2'-O-trans-caffeoylgardoside (14). Conclusion: Compounds 1-4 and 13 are reported from RI for the first time; And UPLC analyses and literature data showe that compounds 5 and 7-13 are originated from Gardenia jasminoides.