ABSTRACT
Saliva is a complex secretion consisting of 99% of water and remaining 1% of organic andinorganic molecules. Sucrose and starches are the predominant dietary carbohydrates in modernsocieties.Among all thefoods consumed by children, chocolates and biscuits are the mostcommon. Therefore this present invivo study was conducted to assess the acidogeniceffect ofcommercially available biscuits on salivary pH among 10 to 15 years old children. Study Designused in the study was In Vivo clinical study (Pilot Trail). The population collected in the survey waschildren between the age group of 10-15 yearsold children. 4 Groups were considered and 10 ineach group. Group 1: Hide and Seek, Group 2: Good Day, Group 3: Dream and Cream, Group 4:Oreo. Sampling method used in the study was conducted as simple random sampling. Ethicalapproval of the study was obtained from Saveetha Institutional Review Board. Informed consent ofthe children were obtained from the parents. Descriptive statistics were expressed by means ofmean and standard deviation. Shapiro Wilks test used to test the normality of the data set.KruskalWallis test was used to find the difference in mean Salivary pH between the groups and within the groups at Baseline, Immediate and after 15min, 30 mins. A statistically significant difference inmean Salivary pH was observed between the groups at Immediate and after 30 mins(p<0.05). Themean Salivary pH was significantly dropped in Oreo, Dream cream and Hide & Seek groups atvarious time-periods. Based on the results of the present study, it can be concluded that maximumdrop in mean salivary pHwas observed in Group IV followed by Group II and Group I. It wasobserved that in all the groups, the pH gradually got back to near normal levels due to the bufferingmechanism of saliva
ABSTRACT
A boca é um ecossistema complexo composto por centenas de espécies microbianas que apresentam características genotípicas e fenotípicas distintas que permitem sua adaptação e sobrevivência às adversidades desse ambiente. Streptococcus mutans é a espécie bacteriana mais relacionada à etiologia da cárie dentária, devido principalmente ao seu potencial acidogênico, acidúrico e na aderência e formação de biofilme dental. Outras espécies bacterianas, como as bifidobactérias, foram detectadas em diversos sítios bucais incluindo lesões cariosas iniciais ou cavitadas e estão sendo relacionadas à etiologia da cárie precoce da infância (CPI). Contrariamente, efeito benéfico na prevenção da doença periodontal tem sido observado para as bifidobactérias pelo fato dessas espécies competirem e interferirem na aderência e formação do biofilme de patógenos periodontais. Assim, ambiguidade em relação ao papel das bifidobactérias na etiologia ou prevenção de doenças bucais está sendo observada na literatura. Este trabalho foi dividido em três capítulos. Os objetivos foram: 1) avaliar a diversidade genotípica e as características fenotípicas de cepas de S. mutans, isoladas do biofilme dental de crianças CPI e CPI-severa (CPI-S) em comparação com crianças livres de cárie (LC); 2) avaliar a capacidade de produzir e tolerar ácidos, de formar biofilme e de induzir lesões iniciais de cárie in vitro por espécies de bifidobactérias comparadas à de espécies bacterianas já reconhecidas no contexto da cárie dentária e 3) investigar o efeito antagonista in vitro de algumas espécies de bifidobactérias sobre biofilmes de Fusobacterium nucleatum, Porphyromonas gingivalis e Streptococcus oralis. No capítulo 1, cepas de S. mutans de amostras de biofilme de crianças com CPI, CPI-S e LC foram isoladas em meio Agar Mitis Salivarius com bacitracina e avaliadas geneticamente pelo método de AP-PCR (Reação em Cadeia da Polimerase usando Iniciadores Arbitrários) e fenotipicamente pelos métodos de...
The mouth is a complex ecosystem composed of hundreds of microbial species with different genotypic and phenotypic characteristics that allow their adaptation and survival in the adversity of that environment. Streptococcus mutans is the bacterial species more closely related to the etiology of dental caries, mainly due to its acidogenic and aciduric potential and adherence and formation of dental biofilm. Other bacterial species, such as bifidobacteria, have been detected in oral sites including initial and cavitated carious lesions and have been related to the etiology of early childhood caries (ECC). In contrast, beneficial effect of bifidobacteria in the prevention of periodontal disease has been observed because they compete and interfere with the adherence and biofilm formation by periodontal pathogens. Thus, ambiguity regarding the role of bifidobacteria in the etiology or prevention of oral diseases has been observed in the literature. This work was divided into three chapters. The objectives were: 1) to evaluate the genotypic diversity and phenotypic characteristics of S. mutans strains isolated from dental biofilm of ECC and severe-ECC (S-ECC) children compared to caries-free children (CF); 2) to evaluate the ability to produce and tolerate acids, to form biofilm and inducing in vitro initial carious lesions by species of bifidobacteria compared to the bacterial species already recognized in the context of dental caries and 3) to investigate the in vitro antagonistic effect of some bifidobacteria species on biofilms of Fusobacterium nucleatum, Porphyromonas gingivalis and Streptococcus oralis. In Chapter 1, S. mutans strains from biofilm samples of children with ECC, S-ECC and CF were isolated in Agar Mitis Salivarius with bacitracin and genetically evaluated by AP-PCR method (Arbitrary Primers - Polymerase Chain Reaction) and phenotypically by acidogenicity methods, verifying the final pH of the cultures after exposure to high glucose concentration...
Subject(s)
Humans , Child, Preschool , Bifidobacterium , Dental Caries , Dental Plaque , Mouth Diseases , Periodontal Diseases , Streptococcus mutans , Actinomyces , Biofilms , Lactobacillus , Porphyromonas , ProbioticsABSTRACT
OBJECTIVES: This study was aimed at evaluating the clinical usability of a new caries activity test (Cariview(R)), which was based on the acidogenic potential of plaque by evaluating it's correlation with the DMFT index and comparing the results of Cariview(R) with those of previous caries activity tests in an adult group. METHODS: Teeth of 74 subjects were examined, and the DMFT index was calculated according to World Health Organization (WHO) criteria to determine the past caries experience. Caries activity tests were performed according to manufacturer's instructions. For Cariview(R) test, plaque samples were collected from the tooth surface by using a sterile cotton swab and incubated at 37degrees C for 48 h; an indicator was added to observe the color changes according to the plaque acidogenicity. The subjects were classified into three groups (Low, Moderate, High) according to the level of caries risk. The data were assessed using one-way ANOVA to compare the caries experiences of risk groups according to the caries activity test results, and the relationships between the caries activity tests and the DMFT index were evaluated. RESULTS: The overall mean DMFT index was 5.70+/-4.42. There was a significant difference in the DMFT index among the caries risk groups (Low, Moderate, High) according to the Cariview(R) test result (P=0.036). Further, there was a positive correlation between the Cariview(R) score and the DMFT index (r=0.23, P=0.047) and between the Cariview(R) score and the previous caries activity tests score (P<0.01). CONCLUSIONS: The new caries activity test (Cariview(R)) can be easily used at dental clinics and enables precise caries risk assessment.
Subject(s)
Adult , Humans , Dental Clinics , Dental Plaque , Risk Assessment , Tooth , World Health OrganizationABSTRACT
Objective: To investigate the acidogenicity and aciduric ability of Streptococcus mutans isolated from the children with different caries susceptibility.Methods: The acidogenic and aciduric ability of Streptococcus mutans strains isolated from children was investigated.Acidogenicity ability was compared among high caries-susceptible group,middle caries-susceptible group and caries-free group.Results: The acidogenicity and aciduric ability of Streptococcus mutans isolated from the high caries-susceptible children and middle caries-susceptible group were significantly higher than that isolated from caries-free group(P