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The present study investigated the material basis of Urtica fissa for the inhibition of benign prostatic hyperplasia(BPH). The active fractions were screened, and the extracts of dichloromethane and ethyl acetate exhibited significantly inhibitory activities against 5α-reductase in vitro and BPH in model rats. The chemical constituents in the active fractions were systematically investigated, and 28 compounds were obtained, which were identified as lobechine methyl ester(1), dibutyl-O-phthalate(2), 1-monolinolein(3), epipinoresinol(4), 5-hydroxy-3,4-dimethyl-5-pentanyl-2(5H)-furanone(5), E-7,9-diene-11-methenyl palmitic acid(6), evofolin B(7), ficusal(8), threo-2,3-bis-(4-hydroxy-3-methoxyphenyl)-3-ethoxypropan-1-ol(9), α-viniferin(10),(9R,7E)-9-hydroxy-5,7-mengatigmadien-4-one-9-O-β-D-glucopyranoside(11), indole-3-carboxaldehyde(12), p-hydroxy ethyl cinnamate(13), benzyl alcohol-O-β-D-glucoside(14), L-methionine(15), 4-methoxyaniline(16), 6-aminopurine(17), 8'-acetyl oilvil(18), 4-methoxyl-8'-acetyl oilvil(19), vanillic acid(20), β-hydroxypropiovanillone(21), 7-hydroxy-6-methoxycoumarin(22), p-hydroxybenzaldehyde(23), pinoresinol(24), erythro-1,2-bis-(4-hydroxy-3-methoxyphenyl)-1,3-propanediol(25), urticol(26), urticol-7-O-β-D-glucopyranoside(27), and lobechine(28). Compounds 1-17 were isolated from U. fissa for the first time. Meanwhile, compound 1 was a new natural product. Compounds 10, 11, 19, 21, and 27 exhibited significant inhibitory effects on 5α-reductase.
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Animals , Rats , Plant Extracts/pharmacology , Prostatic Hyperplasia/drug therapy , Urticaceae/chemistryABSTRACT
Sophora alopecuroides, a plant of the family Leguminosae, is one of the Daodi herbs in Ningxia. The active constituents of Sophora alopecuroides are abundant and complex, including alkaloids, flavonoids, volatile oils, steroids, polysaccharides, fatty acids and so on. In recent decades, a great number of domestic and overseas studies have been carried out on Sophora alopecuroides alkaloids, which have anti-hepatitis, anti-liver fibrosis, anti-cirrhosis, anti-liver failure and anti-liver cancer and other pharmacological effects. Clinically, Matrine-related drugs are used to treat hepatitis B virus infection and other diseases. This review aims to summarize the main active ingredients of Sophora alopecuroides, mainly focusing on the research progress in their treatment activities for liver diseases.
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Liver cancer is a worldwide malignant tumor with an increasing incidence by years. At present， it is facing the predicament of poor prognosis and lack of effective therapeutic drugs. Epimedii Folium is a well-known traditional Chinese medicine with a long history， and exiting clinical and pharmacological studies show that it can be used in clinical treatment of liver cancer. According to reports， Epimedii Folium polysaccharides （EPS）， C-8-isopentenyl substituted flavonoids and their glycosides （icaritin， icariin， baohuoside Ⅰ， epimedin C） have good anti-liver cancer activity. They are the main active ingredients of Epimedii Folium against liver cancer. The data which comes from in vitro and in vivo studies suggests flavonoids in Epimedii Folium demonstrate anti-liver cancer activity through various mechanisms， including inhibiting hepatoma cells proliferation， promoting hepatoma cells apoptosis， improving tumor immunosuppression microenvironment， inhibiting hepatoma cells immune escape， invasion and migration， reversing hepatoma cells resistance， suppressing hepatocellular carcinoma initiation cells and regulating the immunity of the body. While EPS play an anti-hepatocellular carcinoma role mainly through the regulation of immunity. Epimedii Folium exerts good anti-liver cancer effects with multiple components， multiple targets， and multiple pathways， which makes it a valuable anti-liver cancer drug. However， the comprehensive analysis of related aspects is still lacking. Therefore， this study briefly reviewed the anti-hepatocellular carcinoma active ingredients of Epimedii Folium and their mechanisms. In addition， in the process of literature review， it was found that the anti-liver cancer studies of Epimedii Folium mainly focused on a few components and the studies elucidating the active constituents and mechanism of Epimedii Folium against liver cancer on the whole level were insufficient. Based on these questions， the study also proposed corresponding suggestion to provide reference for the further study of substance basis， clinical application and rational development of Epimedii Folium against liver cancer.
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AIM: To screen the active constituents in Taohong Siwu Decoction(THSWD) by Systems Pharmacology and to study its protective effect on myocardial injury through cell experiments. METHODS: The TCMSP database and Pharmmaper database were used to retrieve the constituents and active constituent targets of THSWD. The Genecards database was used to retrieve the myocardial injury related targets gene. Then construct and analyze the constituents-disease intersection target network and the contribution value of constituents, and screen out the main active constituents of THSWD acting on the myocardium. Furthermore, CoCl
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Moringa oleifera is a typical tropical multi-functional fast-growing tree species， which is native to India and now widely planted in various tropical regions. After some research and textual research， the records of M. oleifera in China can be traced back to the The Bower Manuscript（volume Ⅱ）（about the 4th—6th century A.D.） unearthed in Kuqa， Xinjiang. M. oleifera contains a variety of active ingredients such as flavonoids， alkaloids， glycosides， organic esters and rich nutrients， which has outstanding medicinal and economic value. As a result， M. oleifera is widely used in food additives， water purification agents， medicine and health care and other fields. In 2012， the Ministry of Health approved M. oleifera as a new resource food. In the current trend of medicine diet and health preservation of traditional Chinese medicine， M. oleifera stands out， which not only expands the direction for the development of health products， but also enriches the development demand of traditional Chinese medicine in our country. However， in our country， the research on M. oleifera is mainly focused on the extraction of chemical components， analysis of nutritional components， consumption and content determination， and the research on its composition and pharmacological action is one-sided. For this purpose， this paper briefly reviewed the active components， hypoglycemic and treatment of diabetic complications， hypolipidemic， antioxidant， anti-inflammatory and applied research status of M. oleifera， and looked forward to the future development and utilization of M. oleifera leaves in China.
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The discovery of active constituents of traditional Chinese medicine(TCM) faces multiple challenges, such as limited approaches to evaluate poly-pharmacological effects, and the lack of systematic methods to identify active constituents. Aimed at these bottleneck problems in the field, the present study intensively discussed the key scientific problems in the identification of active constituents of TCM, based on scientific methodologies including systematology, information theory, and synergetics. A comprehensive strategy is herein proposed to investigate the correlations between the chemical composition and biological activities of TCM, from macro-, meso-, and micro-scales. Moreover, in this study, we systematically proposed the methodology of the multimodal identification of TCM active constituents, and thoroughly constructed its core technologies. Its technical framework is suggested to be assessed by multimodal information acquisition, centered on multisource information fusion, and focused on interaction evaluation. Furthermore, the core technologies for the multimodal identification of active constituents of TCM were developed in this study, which is according to the characteristics of the exchanges of between TCM and biological organisms, in the aspects of material, energy and information. Finally, two examples of the application of the proposed method were briefly introduced. The proposed methodology provides a novel way to solve the bottlenecks in the study of active constituents of TCM, and lays the foundation for the multimodal study of TCM.
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Chemistry, Pharmaceutical/methods , Drugs, Chinese Herbal/chemistry , Medicine, Chinese Traditional , Research DesignABSTRACT
Schisandrae Chinensis Fructus is dry,mature fruits of magnolia plant Schisandra chinensis. In recent years,domestic and foreign scholars have made a lot of studies on active constituents of S. chinensis and pharmacological activities. This article systematically organizes the active constituents and pharmacological activities of S. chinensis. It was found that fruit,seeds,roots,stems and leaves of S. chinensis and other medicinal parts mainly contained lignans,volatile oils,triterpenoids,polysaccharides and flavonoids. And lignans were the main characteristic active ingredients of S. chinensis,triterpenoids,polysaccharides and volatile oils were the secondary active ingredients,and the activity of flavonoids had rarely been reported. According to the pharmacological study of S. chinensis,S. chinensis lignans had certain effects on the central nervous system,cardiovascular system,liver,kidney and reproductive system,with anti-oxidation,anti-tumor,anti-bacterial and anti-HIV effects. Other chemical components also had good pharmacological activities,but were less studied than lignans. With dual functions in medicine and food, it was widely used as a health product and medicine. This article systematically summarized the active constituents and pharmacological activities of S. chinensis,and provided an important reference and basis for the further development of the health product and medicine of S. chinensis in the future.
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Aim To explore the anticomplementary ac-tivity and active constituents of Phyllanthus urinaria. Methods Being guided by bioactive screening,the anticomplementary constituents of P.urinaria were iso-lated and purified by solution partition and chromato-graphic techniques,and their structures were identified by 13C-NMR spectrum and the comparison of reported data. The anticomplementary activity and possible mechanism were assayed.Results The EtOAc fraction of the methanol extract of P.urinaria was shown to be the active fraction,and two compounds were purified from the fraction and were identified as corilagin and ellagic acid.The EtOAc and the two compounds signifi-cantly inhibited the hemolysis of the complement classi-cal pathway,with IC50values of 53.77 mg·L-1, 176.54 mg·L-1and 102.23 mg·L-1,respectively. While they just showed slight effect on inhibiting the hemolysis of the complement alternative pathway. All of them affected the formation of C3 convertase of the classical pathway. Conclusions The polyphenols are main anticomplentary constituents of P. urinaria,and its mechanism is related to inhibiting formation of C3 convertase of the classical pathway.
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OBJECTIVE:To establish quantitative analysis of HPLC-quantitative analysis of multi-components by single marker (QAMS) for simultaneous determination of 6 active constituents in Compound huangqin tablets. METHODS:Using baicalin as internal content,HPLC method was used to calculate relative correction factor (RCF) of baicalin to polydatin,wogonoside, baicalein,andrographolide and dehydroandrographolide. The contents of above-mentioned 5 components in Compound huangqin tablets were calculated by using RCF(calculation value). The external standard method(ESM)was used to determine the contents of 6 constituents (measured value). The differences between calculation value and measured value were compared. The determination was performed on ZORBAX Eclipse XDB C18 column with mobile phase consisted of acetonitrile-0.05％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min. The detection wavelength was set at 306 nm for polydatin,225 nm for andrographolide,275 nm for baicalin,wogonoside and baicalein,254 nm for dehydroandrographolide. The column temperature was set at 25 ℃ and the injection volume was 10 μL. RESULTS:The linear range was 4.555-81.99 μg/mL for polydatin, 47.03-846.5 μg/mL for baicalin,12.73-229.1 μg/mL for wogonoside,12.88-231.8 μg/mL for baicalein,2.632-47.38 μg/mL for andrographolide,3.508-63.14 μg/mL for dehydroandrographolide (r=0.9992-0.9997),respectively. Limits of detection were 2.25,6.92,1.76,4.69,1.37,1.51 ng;limits of quantitation were 6.89,19.98,5.28,13.81,4.15,4.71 ng. Average recoveries were 98.2％,98.8％,97.9％,98.9％,98.8％,98.7％(RSD=0.91％-1.32％,n=9). RSDs of precision,reproducibility and stability tests (48 h) were lower than 2.0％ (n=6 or n=7). RCFs of baicalin to polydatin, wogonoside, baicalein, andrographolide and dehydroandrographolide were 0.529,0.506,1.004,0.831 and 1.087,respectively. There was no statistical significance between calculated values and measured ones(P>0.05). CONCLUSIONS:The method is simple,effective,accurate and cost saving. It can be used for simultaneous determination of 6 active constituents in Compound huangqin tablets.
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As a most effective monomer composition from bark of Pacific Yew,paclitaxel and its derivatives are used in clinical practice as broad spectrum anticancer drugs.Since its discovery in the 1970 s,many researches had been carried out,mainly focusing on the modification,structure-activity relationship and pharmacological activity.The great successes pressed ahead the development of a series of taxol-like drugs,including taxol,docetaxel,cabazitaxel,larotaxel.Nowadays,studies of taxol are still the hotpots,which concentrated on the new source such as cultivation of tissue,fungus culture and new dosage forms.As the representative of drugs research from natural source,taxol is worth to be summarized of its history and ongoing development for looking forward to bring new innovation mentality in new drugs.
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AIM To study the correlations between colors and contents of nine active constituents of Salvia miltiorrhiza Bge.with four growth years (one,two,three and five years).METHODS The chromatic values (L*,a* and b*) were determined by precise color difference meter.HPLC was applied to determining the contents of water-soluble constituents (lithospermic acid,salvianolic acid B,rosmarinic acid,protocatechuic aldehyde acid and salvianic acid A sodium) and liposoluble-soluble constituents (dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ A).Then the correlations between colors and contents of various constituents were investigated by Pearson correlation analysis.RESULTS L* and b * values of two growth-year S.miltiorrhiza,together with a* value of three growth-year S.miltiorrhiza,were the highest,but various chromatic values of five growth-year S.miltiorrhiza were all the lowest.The contents of various constituents in three growth-year S.miltiorrhiza reached the highest.Lithospermic acid and salvianic acid A sodium showed no significant correlations with various chromatic values,salvianolic B,rosmarinic acid and tanshinone Ⅱ A exhibited significantly positive correlations with them,and protocatechuic aldehyde had significantly negative correlations with them.CONCLUSION The quality of three growth-year S.miltiorrhiza is relatively high.
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In this study, the chemical constituentsfrom Valeriana amurensis AD-effective fraction were investigated based on the effect of Valeriana amurensis on Alzheimer's disease (AD) in previous study. Valeriana amurensis was extracted with 75% ethanol and the obtained extract were extracted and subjected to AB-8 macroporous resin column to obtain the AD-effective fraction of Valeriana amurensis. 9 compounds (1-9) were isolated with silica gel, ODS column chromatography and preparative HPLC. The structures of these compounds were determined as 6-hydroxy-7-(hydroxymethyl)-4-methylenehexahydrocyclopenta[c]-pyran-1(3H)-one (1), suspensolide F (2), loganin(3), α-morroniside(4), β-morronisid (5), partinovalerosidate (6), zansiumloside A (7), (-)-angelicoidenol-2-O-β-D-glucopyranoside (8), citroside A (9). Compounds 6-9 were isolated from the valerian genus for the first time and further investigated the anti-AD effect of compounds 1-9 in vitro found that compound 2 and 6 protected the PC12 cells from injury significantly.
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The scientific interpretation of the effectiveness of traditional Chinese medicine (TCM) is a communication channel between Chinese medicine and modern medicine. The evaluation of TCM efficacy is an important prerequisite for discovering active constituents. Therefore, there is an urgent need to establish a biological language for scientific explanation of the effectiveness of TCM, and modern scientists can further accept the significant value of TCM theory and its clinical practice. The syndrome and corresponding formulation are two important parts in TCM, and they are directly related to the effectiveness of TCM. Our team has taken syndrome and formulation as the research objects, integrated serum pharm-chemistry of TCM with metabolomics technology, developed a new platform termed Chinmedomics, which is capable of evaluating effectiveness of TCM and discovering the syndrome biomarkers as well as the effective substances. The correlation between the endogenous biomarkers of syndrome and exogenous constituents of formulation is analyzed to find the highly associated compounds as the effective substances, and further clarifying their activities, and may discover lead compounds. This effective strategy could speed the drug discovery from natural resources. This article systematically introduced the establishment and application of Chinmedomics theory, in order to provide references for studies on TCM, and some characteristic examples are presented to highlight the application of this new strategy for discovering the potential active constituents of TCM.
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Aqueous two-phase system (ATPS) is a new liquid-liquid fractionation technique that has been extensively applied in the separation and purification of natural products including proteins, genetic material, bionanoparticles, cells, and secondary metabolites. Advantages of the technique include scale-up potential, continuous operation, ease of process integration, low toxicity, etc. This paper reviewed the applications in separation and purification of the active constituents from Chinese materia medica (CMM) in recent five years, so as to promote the development of separation of the active constituents in CMM.
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Objectives: To investigate the anti-inflammatory components from the leaves of Liriodendron chinensis. Methods: The 95% alcohol extract from the leaves of L. chinensis was subjected to column chromatography, and the structures of purified compounds were determined by spectral methods. The bioassay was performed through the inhibitory effects on the inflammatory cells activated by lipopolysaccharide (LPS). Results: Nine compounds were isolated, including octacosanoic acid (1), stearic acid (2), (2 R)-2-hydroxy-N-[(2 S,3 S,4 R,8 E)-1,3,4-tri-hydroxyicos-8-en-2-yl]tetracosanamide (3), (2 R)-2-hydroxy-N-[(2 S,3 S,4 R,8 E)-1-O-ß-D-glucopyranosyloxy-3,4-dihydroxyoctadec-8-en-2-yl]eicosanamide (4), (2 R)-2-hydroxy-N-[(2 S,3 S,4 R,8 E)-1-O-ß-Dglucopyranosyloxy-3,4-dihydroxyoctadec-8-en-2-yl]hexadecanamide (5), dicentrinone (6), liriodenine (7), daucosterol (8), and liriodendritol (9) and among which five compounds could significantly lower the content of nitric oxide (NO) from peritoneal macrophages of rats induced by LPS and reduce the splenic lymphocyte proliferation in mice. This is the first report on the occurrence of ceramides and dicentrinone in the plants of Liriodendron Linn. Conclusion: The five compounds are likely to be anti-inflammatory compounds concerning to their pronounced inhibitory action on the activated inflammatory cells. This assessment might provide a basis for searching the potent active compounds used for the treatment of inflammation.
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Changium smyrnioides (C . smyrnioides) Wolff is one species of monotypic genus in the A piaceae family w hich is a Chinese endemic species medicinal plant .It has the effect of nourishing the body ,improving health ,moiste‐ning the lung ,eliminating sputum and suppressing cough ,regulating stomach and relieving vomiting ,neutralizing toxins and subsiding swelling .C .smyrnioides as one kind of medicinal food and tonic agents ,is one of the important export medicinal ma‐terials .The tonic effect may be related with immuno‐regulatory ,anti‐fatigue and anti‐stress activities ,and polysaccharides are the main active constituents .The progress of the active constituents and pharmacological actions of C .smyrnioides are re‐viewed to provide basis for future rational development and utilization .
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Aim: To evaluate the antibacterial activity of selected medicinal plants from South- Eastern Nigeria against ESBL producing Pseudomonas species. Study Design: Agar well diffusion assay for determination of sensitivity and Agar dilution method for determination of MIC were used. Place and Duration of Study: Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Anambra State, an Igbo speaking South-Eastern State in Nigeria, between February 2010 and october 2010. Methods: The antibiograms of ten (10) ESBL producing Pseudomonas species to selected antibiotics as well as the antibiograms of these isolates against methanol leaf extracts of Anthocleista djalonensis A Chev. (Loganiaceae) (MLEA) (Igbo name- Uvuru or Ayuu), Zapoteca portoricensis H. M. Hem. (Fabeceae/mimosidea) (MLEZ) (Igbo name- Ayuu), Gongrenema latifolium Benth. (Asclepiadaceae) (MLEG) (Igbo name- Utazi) and Psidium guajava Linn. (Myrtaceae) (MLEP) (Igbo name- Gova), using doses of 3000.00 to 21.87 mg/ml using agar disk diffusion and agar well diffusion assays respectively were determined. The MIC of the plant extracts in comparison with that of gentamicin were also evaluated using the agar dilution method. Results: All the ESBL producing Pseudomonas spp. were multi-drug resistant (IZD = 0) but, were all sensitive to imipenem. Only two strains of Pseudomonas monteilli were sensitive to MLEG with sensitivity decreasing with increasing concentrations of the MLEG. All the ESBL producing Pseudomonas spp were sensitive to MLEZ and MLEP. Also, sensitivity decreased with increasing concentration of the MLEZ and MLEP. MLEA showed no antimicrobial activity against the tested ESBL producing Pseudomonas spp. Gentamicin, with an MIC of 0.00015 μg/ml, was more active than the plant extracts. The MLEP was the more active with an MIC of 1 - 4.37 mg/ml, than MLEZ and MLEG which had MICs of 150 and 75 mg/ml respectively. Active constituents of these plant extracts especially that of Psidium guajava, may thwart the emerging resistance to carbapenems. Conclusion: Development of a complex mixture of the active constituents or single active constituent(s) of Psidium guajava as antimicrobial agent(s) that will be effective against ESBL producing Pseudomonas species.
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Objective: To explore the suitable packaging methods for Rhei Radix et Rhizoma (RRR) with the contents of active ingredients, quality loss before and after storage, shelf life, and economic benefit as indexes. Methods: Rheum palmatum was used in the experiment and the color of RRR was studied according to Chinese Pharmacopeia 2010. The extract was obtained by hot-dip method, and the contents of anthraquinone, gallic acid, and catechin were determined using HPLC. Results: The moisture capacity of RRR package had the significant effect on the active constituents, the highest content of extracting substances (31.36%) was obtained with the moisture content of 24%, and the lowest content was obtained with the moisture content of 14%. The highest anthraquinone content (1.893%) was obtained with the moisture content of 14%, and the highest in catechin and gallic acid contents were 1.492% and 0.400% with the moisture content of 20%. The contents of RRR extract and anthraquinone were the highest (33.47% and 1.891%) with vacuum packing, while those with common package were the lowest (28.30% and 1.539%). The highest contents of gallic acid and catechin (0.323% and 2.022%) were obtained with deoxidizer packaging. The mildew and infestation observation and the quality determination of one-year stored RRR showed that the vacuum packing had no mildew with the moisture content of less than 20%. Conclusion: The vacuum packing is an optimal atmosphere packaging method, which could increase the revenue of farmers and the moisture content of RRR during storage.
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Sodium alginate, irradiated by Co-60 gamma rays in solid state, elicits plant growth promoting responses in various plants. Irradiated sodium alginate (ISA) was applied as a foliar spray on mint (Mentha arvensis L.) to investigate its effect on plant growth, physiological attributes and herbage yield as well as on content and yield of essential oil and its components (menthol, L-menthone, isomenthone and methyl acetate). A simple pot experiment was conducted applying five concentrations of ISA, viz. 25, 50, 75, 100 and 125 mg L-1, as foliar sprays. GPC study revealed formation of lower molecular weight oligomer fractions in irradiated samples which could be responsible for plant growth promotion in the present work. Of the five ISA concentrations, 100 mg L-1 proved the best. As compared to the control, the ISA applied at 100 mg L-1 resulted in the highest values of all physiological parameters at 100 and 120 days after planting.
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A técnica de análise derivativa de dados espectrais foi usada para estudar a variação dos constituintes opticamente ativos (COAs) na água, por meio de dados de campo e de imagens do sensor orbital Hyperion/EO-1. A imagem Hyperion usada neste estudo foi adquirida no dia 23 de junho de 2005, no final do período de cheia. Uma campanha de campo foi realizada entre 23 e 29 de junho de 2005, para coletar dados espectrais e limnológicos in situ. A imagem foi pré-processada visando eliminar faixas de pixels anômalos e convertida de valores de radiância para reflectância de superfície, portanto, corrigidos dos efeitos de absorção e espalhamento atmosféricos. Uma análise da correlação foi realizada para examinar a associação da reflectância e de sua primeira derivada espectral com as concentrações dos COAs. Melhores resultados foram obtidos após a diferenciação dos espectros, o que ajudou a reduzir a influência de efeitos indesejáveis, provindos de diferentes fontes de radiância, sobre as medidas de reflectância da superfície da água realizadas em ambos os níveis de aquisição de dados. Por meio de ajustes de regressões empíricas, considerando o conjunto de dados Hyperion, a primeira derivada espectral em 711 nm explicou 86 por cento da variação da concentração de sedimentos inorgânicos em suspensão (µg.l-1) e a primeira derivada espectral em 691 nm explicou 73 por cento da variação na concentração da clorofila-alfa (µg.l-1). As relações de regressão foram não-lineares, pois, em geral, as águas que se misturam na planície de inundação Amazônica se tornam opticamente complexas. A técnica de análise derivativa hiperespectral demonstrou potenciais para mapear a composição dessas águas.
Derivative analysis of spectral data was used as a technique to study the variation of optically active constituents (OACs) of water, using field data and hyperspectral imagery of EO-1 Hyperion orbital sensor. The Hyperion image used in this study was acquired on June 23, 2005, at the end of the high water period for the Amazon River. A field campaign was carried out between June 23 and 29, 2005 to collect spectral and limnological in situ data. The image was pre-processed to remove stripes of abnormal pixels and converted from radiance to surface reflectance values, thus, correcting the effects of atmospheric absorption and scattering. A correlation analysis was carried out to examine the association of the spectral reflectance and its first derivative to the concentrations of OACs. Better results were obtained after spectra differentiation, which helped to reduce the influence of undesirable effects, coming from different sources of radiance, on the measurements of water surface reflectance taken at both data acquisition levels. Through empirical regression fits, considering the Hyperion dataset, the first spectral derivative at 711 nm explained 86 percent of the variation of suspended inorganic sediment concentration (µg.l-1), and the first derivative at 691 nm explained 73 percent of the variation of chlorophyll-a concentration (µg.l-1). The regression relations were nonlinear because, generally, the water masses that mix in the Amazon floodplain become optically complex. The hyperspectral derivative analysis demonstrated potential for mapping the composition of these waters.