ABSTRACT
The objective was to investigate the hypoglycemic action of catalpol in spontaneous diabetes db/db mice. 40 db/db mice were randomly divided into fi ve groups: model control gourp; db/db plus catalpol 40, 80, 120 mg/kg body wt. groups and db/db plus metformin 250 mg/kg group. Age-matched db/m mice were selected as normal control group. The mice were administered with corresponding drugs or solvent by gavage for 4 weeks. The oral glucose tolerance test was carried out at the end of 3rd week. After 4 weeks of treatment, the concentrations of fasting blood glucose (FBG), glycated serum protein (GSP), insulin (INS), triglyceride (TG), total cholesterol (TC) and adiponection (APN) in serum were detected. The protein expressions of phosphorylation-AMPKalpha1/2 in liver, phosphorylation-AMPKalpha1/2 and glucose transporter-4 (GLUT-4) in skeletal muscle and adipose tissues were detected by western blot. Real time RT-PCR was used to detect the mRNA expressions of acetyl-CoA carboxylase (ACC) and Hydroxymethyl glutaric acid acyl CoA reductase (HMGCR) in liver. Our results showed that catalpol could significantly improve the insulin resistance, decrease the serum concentrations of INS, GSP, TG, and TC. The concentrations of APN in serum, the protein expression of phosphorylation-AMPKalpha1/2 in liver, phosphorylation-AMPKalpha1/2 and GLUT-4 in peripheral tissue were increased. Catalpol could also down regulate the mRNA expressions of ACC and HMGCR in liver. In conclusion, catalpol ameliorates diabetes in db/db mice. It has benefi t eff ects against lipid/glucose metabolism disorder and insulin resistance. The mechanism may be related to up-regulating the expression of phosphorylation-AMPKalpha1/2.
Subject(s)
Animals , Mice , Acetyl-CoA Carboxylase , Acyl Coenzyme A , AMP-Activated Protein Kinases , Blood Glucose , Blotting, Western , Cholesterol , Fasting , Glucose , Glucose Tolerance Test , Insulin , Insulin Resistance , Liver , Metabolism , Metformin , Muscle, Skeletal , Oxidoreductases , RNA, Messenger , TriglyceridesABSTRACT
Objective:To investigate the hpyerglycemic action of arctiin in db/db mice with spontaneous diabetes and the underly-ing mechanism. Methods:Totally 40 db/db mice were randomly divided into five groups: the model control group, arctiin group re-spectively with the dose of 75, 150, 300 mg·kg-1 , 300 mg·kg-1 metformin group. The age-matched db/m mice were selected as the normal control group. The mice were administered with corresponding drugs or solvent by gavage for 4 weeks. The oral glucose tol-erance test was carried out at the end of the 3rd week. After the 4-week treatment, all the mice were fasted overnight (12h), and then the body weight and fasting blood glucose ( FBG ) were determined. The concentration of insulin ( INS ) , glycated serum protein ( GSP) , triglyceride ( TG) , total cholesterol ( TC) and adiponection ( APN) were detected. Results:Arctiin could significantly lower the body weight and FBG, improve the glucose tolerance, decrease the serum concentration of INS, GSP, TG, TC and APN(P<0. 05 or 0. 01). Conclusion:Arctiin has benefit effects against glucose/lipid metabolism disorder and insulin resistance in db/db diabetic mice. The mechanism may be related to up-regulating the expression of adiponection.
ABSTRACT
@#Objective To observe the effect of lycium barbarum polysaccharide (LBP) on insulin resistance and mRNA expression of adiponection gene in perirenal adipose tissue of type 2 diabetes mellitus (2-DM) rats.Methods The 2-DM model of rat was established by high-fat diet and streptozocin. The 2-DM model rats were randomly divided into the model group and high, middle and low doses of LBP groups with 10 animals in each group, another 10 rats were selected as the control group. The mRNA expression of adiponection gene in perirenal adipose tissue was determined by RT-PCR. The levels of fasting blood glucose (FBG), fasting insulin (FINS) and blood lipid total cholesterol (TC), triglycerides (TG), high density lipoprotein (HDL) and low density lipoprotein (LDL) were detected and insulin resistance index (IRI) was calculated after rats fed eight weeks.Results The mRNA expression of adiponection gene increased in the middle and high doses of LBP groups, while the levels of FBG, FINS, IRI, TC, TG and LDL decreased in different doses of LBP groups, significantly different from those in 2-DM model group.Conclusion LBP can improve the metabolism disorder of blood lipid and decrease insulin resistance by increasing mRNA expression of adiponection gene in 2-DM rats.
ABSTRACT
0.05), but insulin sensitivity was actually improved (P