ABSTRACT
Roots of plants have immense reducing potential. Ions of noble metals namely Au3+ and Ag+ get reduced easily to form Au and Ag nanoparticles (NPs), respectively. Therefore, we hypothesize that plant roots could have potential to form Au-NPs and Ag-NPs. For present investigations, plants of Pennisetum glaucum L. were used to evaluate if their roots possess capacity to generate metal NPs. The generation of Au-NPs and Ag-NPs was initially presumed based on colour change, and confirmed by UV-vis spectra, TEM and EDX investigations. Pale yellow Au3+ and colourless Ag+ solutions turned purple and brown, respectively, by roots of Pennisetum sp. within 8 h. Absorption spectra of respective solutions showed plasmon resonance band at 560 nm and 420 nm confirming the presence of Au-NPs and Ag-NPs. TEM coupled with SAED revealed the presence of crystalline spherical NPs in the size range of 5-50 nm in these solutions. EDX further confirmed the presence of Au and Ag as NPs of respective solutions. These results confirmed that the roots of P. glaucum possess ideal reducing strength to generate Au-NPs and Ag-NPs exogenously in the aqueous phase.
ABSTRACT
Aim of our study is to evaluate the antibacterial activity of silver Nano particle(Ag-NPs) against MRSA strains recovered from patients with oral infections Material and Methods:- Samples were collected from Patients attending department of oral and maxillofacial surgery, Jaipur dental college who were diagnosed with oral infections. The samples were subjected to culture and sensitivity and MRSA (Methicillin resistant Staphylococcus aureus) screening. MRSAstrains were treated with Ag-Nanoparticles to know the antibacterial affect Results:- Out of 146 samples, 26 specimens showed Methicillin-resistant S aureus (MRSA) The MIC and MBC values of Ag-NPs against MRSA strains were observed to be very low (i.e. in the range of 12.5-100 μg/ml), indicating very well bacteriostatic (represented by the MIC) and bactericidal activity (represented by MBC). Conclusion:- In recent years, there is increase in MRSA in oral and perioral samples with emergence of new resistant strains of MRSA. In our study we have seen Ag-NPs size 5-10nm has inhibited growth of Methicillin Resistant Staphylococcus aureus in vitro at nontoxic concentration, which makes it to use as anti-bacterial agent in oral infections
ABSTRACT
This article reports an ecofriendly approach for the synthesis of Ag nanoparticles (Ag NPs) using aqueous leaf extract of Curcuma aromatica. The synthesized Ag NPs were characterized using UV-visible spectroscopy, FTIR, XRD and TEM analyses. UV–Visible spectra of the aqueous medium containing Ag NPs showed a gradual decrease of the absorbance peak observed at 442 nm. The biomolecules responsible for the reduction of Ag NPs were analyzed by FTIR. XRD result confirmed the presence of AgNPs with FCC crystal structure. The calculated crystalline size using Scherrer formula is in the range from 20 to 80 nm. TEM analysis ascertained spherical nature of the Ag NPs. The synthesized Ag NPs exhibited good antibacterial performance against gram-positive and gramnegative bacteria strains, which was studied using standard disc diffusion method. The synthesis of Ag NPs by this method is rapid, cost effective and can be used for various applications.
ABSTRACT
Objective To establish a paper-based surface-enhanced Raman scattering (SERS) method for the detection of dyed K adsura longipedunculata Finet et Gagnep .Methods Supported silver nanoparticles on filter paper were synthesized simply by soaking method .In addition ,factors including enrichment ratio of silver nanoparticles ,the enhancement effect and sta-bility of the SERS device were investigated .Results The K adsura longipedunculata Finet et Gagnep ,dyed by Erythrosine or Acid Red at low concentration had been detected successfully .Conclusion Combined with the paper device and SERS ,the ap-proach was rapid and non-destructive which could be used to identification of dyed K adsura longipedunculata Finet et Gagnep .