ABSTRACT
OBJECTIVE: To explore the response intensity of reactive oxygen species(ROS) components including H2O2, O-2 and OH- in the course of wound-induced sesquiterpenes formation in Aquilaria sinensis callus. METHODS: The synthesis of sesquiterpenes in wounded callus was analyzed by using GG-MS technique. Total ROS distribution in cells was observed by fluorescence microscope after H2DCF fluorescent probe staining. The H2O2, O-2 and OH- contents were measured according to biochemical reaction principle. RESULTS: Two sesquitterpenes were synthesized and accumulated in the wounded cells. As a signal molecule, ROS burst and accumulated in the process of wound-induced sesquiterpenes formation, DCF fluorescence was observed to increase gradually. DCF fluorescence reached peak at 3 d and then began to decrease. The response of H2O2 and O-2 to injury took place earlier than those of OH-, H2O2 and O-2 reached the maximum value at 3 d and then decreased, while OH- reached the maximum value at 5 d. CONCLUSION: All ROS components respond to injury stimulation as signaling molecules, and the difference in response intensity of each component can synergistically regulate defense response to promote the synthesis of sesquiterpenes in A.sinensis callus.